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The map label for this gene is gatB

Identifier: 54019989

GI number: 54019989

Start: 36215

End: 37615

Strand: Direct

Name: gatB

Synonym: mhp030

Alternate gene names: 54019989

Gene position: 36215-37615 (Clockwise)

Preceding gene: 54019988

Following gene: 54019990

Centisome position: 4.06

GC content: 26.2

Gene sequence:

>1401_bases
ATGAATAATAAATATTTAGTCACAATTGGGATTGAAATTCATTTGGAATTAAATACAAAAGCAAAAATGTTTTCAAATTC
ACCGAATTTACGCGGTGAAGCAAATCAATTTTTTAATCTTTTTGATCTAGCTTATCTCGGAACTTTACCAAAAATTAATA
AAGAAGCTGTTAAAAAAGCAATAATTTTGGCAAAAGCACTAAAAATGCAAATTTCACCAATTTTAGCTTTTGATCGAAAA
AATTATTTCTACCCTGATTTGCCAAAAGGTTTTCAAATTAGTCAACAATTCCATCCCATTGGAAAAAATGGAATTTTAAA
AGTTGGTGATTTTAATGTTGAAATTGAAAGAATTCATCTTGAAGAAGATACGGCAAAACAAATTCATAAAGATAATCAAA
CATTTTTTGATTATAATAGATGTGGGGTTCCATTGATTGAAATTGTTACAAGACCTGTAATAAGTTCATCAAAACTAGCA
GCAAAATATGTCGATGAGATTAAAAAATTAGCCTTATTTTTAGGTATTTCTGATGCAAAATTAGAAAATGGATCACTTCG
TGCTGATATAAATATTTCAGTTCGCCCTGAAAATCAAGTCGAATTTAATCCAAAAGTTGAAATAAAAAATATAAATTCAA
TTTCTAATATCCAAAAAGCCGCCGAACTTGAAATTCAAGAACAAATTTATGCCTATGAAAACGGTATAAAAGTCAAGCAA
GTTACAAAAAAATTCAATGATGTTATTGCAAGAAATCAAATTTTAAGACTTAAAACAGATGCAATTGACTATAAATATTT
TCCAGAACCTAATTTGCCATATATTGAACTAAAGCCAAATTTTGTTGATAAAATTCAGGTTCCACTTACCCCATCACAAA
TAGAAAAAATGCTTAAAAAAGAAGGAGTTTCCACCTTTTATATTAATCAGATTTTGAATAATTTTAACTACTGAACCTAC
TTAAACAACTCCAATCCTGATAATTGAAGTTTAAGTGTTAAATTATTTTTTTCTGAAATTGTACCAATTATTAATAAGCT
AGGGTTCGAAAATTTAGCAATAGAATTTGATTTTTTTGGTAAAATAATCCAAAAACATTTTAACAAAGAATTGTCAAATT
CCGAAATTCGCAAAATAATTGAAATAAAACAAACAAACAAACAATTTAATTTAAATGATATTTTATCTAAGATAAAGGAG
AAAAAACTATCTGAAGCAGCAATAAAAAACTTGTTAAAAGAAGTAGTTAGTGAAGAATTTGCTCAGATTGAAAAAAATAG
ACAGGATAAGTCTAAATTATTTAAATTTTTAATAGGAAAATTAATGCAAAAAACAAAAGGAAACGCCGAACCAATTAAAG
TCAATCAAGTTTTAAAAGAATGGTTAGATAGTTTTAACTAA

Upstream 100 bases:

>100_bases
AATTTGCCTTTTAATTTAGCGGTTGATTCAAAAATAAATTCTGATGCAGAATTGTTAGGTTTTTCCCTTTATCTTGAAGA
AAAAATAAGCGAAATTAATA

Downstream 100 bases:

>100_bases
AAAATAAGTAAAATATTACAGAGACAAAATTGTTGGTTATTTTTCTAGGTAAAACTCAGGATTTTCCAAAAAAATTAATT
TATTTTTTTGGAAAATTAAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNNKYLVTIGIEIHLELNTKAKMFSNSPNLRGEANQFFNLFDLAYLGTLPKINKEAVKKAIILAKALKMQISPILAFDRK
NYFYPDLPKGFQISQQFHPIGKNGILKVGDFNVEIERIHLEEDTAKQIHKDNQTFFDYNRCGVPLIEIVTRPVISSSKLA
AKYVDEIKKLALFLGISDAKLENGSLRADINISVRPENQVEFNPKVEIKNINSISNIQKAAELEIQEQIYAYENGIKVKQ
VTKKFNDVIARNQILRLKTDAIDYKYFPEPNLPYIELKPNFVDKIQVPLTPSQIEKMLKKEGVSTFYINQILNNFNYWTY
LNNSNPDNWSLSVKLFFSEIVPIINKLGFENLAIEFDFFGKIIQKHFNKELSNSEIRKIIEIKQTNKQFNLNDILSKIKE
KKLSEAAIKNLLKEVVSEEFAQIEKNRQDKSKLFKFLIGKLMQKTKGNAEPIKVNQVLKEWLDSFN

Sequences:

>Translated_466_residues
MNNKYLVTIGIEIHLELNTKAKMFSNSPNLRGEANQFFNLFDLAYLGTLPKINKEAVKKAIILAKALKMQISPILAFDRK
NYFYPDLPKGFQISQQFHPIGKNGILKVGDFNVEIERIHLEEDTAKQIHKDNQTFFDYNRCGVPLIEIVTRPVISSSKLA
AKYVDEIKKLALFLGISDAKLENGSLRADINISVRPENQVEFNPKVEIKNINSISNIQKAAELEIQEQIYAYENGIKVKQ
VTKKFNDVIARNQILRLKTDAIDYKYFPEPNLPYIELKPNFVDKIQVPLTPSQIEKMLKKEGVSTFYINQILNNFNY*TY
LNNSNPDN*SLSVKLFFSEIVPIINKLGFENLAIEFDFFGKIIQKHFNKELSNSEIRKIIEIKQTNKQFNLNDILSKIKE
KKLSEAAIKNLLKEVVSEEFAQIEKNRQDKSKLFKFLIGKLMQKTKGNAEPIKVNQVLKEWLDSFN
>Mature_466_residues
MNNKYLVTIGIEIHLELNTKAKMFSNSPNLRGEANQFFNLFDLAYLGTLPKINKEAVKKAIILAKALKMQISPILAFDRK
NYFYPDLPKGFQISQQFHPIGKNGILKVGDFNVEIERIHLEEDTAKQIHKDNQTFFDYNRCGVPLIEIVTRPVISSSKLA
AKYVDEIKKLALFLGISDAKLENGSLRADINISVRPENQVEFNPKVEIKNINSISNIQKAAELEIQEQIYAYENGIKVKQ
VTKKFNDVIARNQILRLKTDAIDYKYFPEPNLPYIELKPNFVDKIQVPLTPSQIEKMLKKEGVSTFYINQILNNFNY*TY
LNNSNPDN*SLSVKLFFSEIVPIINKLGFENLAIEFDFFGKIIQKHFNKELSNSEIRKIIEIKQTNKQFNLNDILSKIKE
KKLSEAAIKNLLKEVVSEEFAQIEKNRQDKSKLFKFLIGKLMQKTKGNAEPIKVNQVLKEWLDSFN

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=289, Percent_Identity=37.3702422145329, Blast_Score=191, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI115534094, Length=321, Percent_Identity=34.2679127725857, Blast_Score=191, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6319391, Length=318, Percent_Identity=34.2767295597484, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI45550808, Length=495, Percent_Identity=25.6565656565657, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53466; Mature: 53466

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKYLVTIGIEIHLELNTKAKMFSNSPNLRGEANQFFNLFDLAYLGTLPKINKEAVKKA
CCCCEEEEEEEEEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
IILAKALKMQISPILAFDRKNYFYPDLPKGFQISQQFHPIGKNGILKVGDFNVEIERIHL
HHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCEEEEEEEE
EEDTAKQIHKDNQTFFDYNRCGVPLIEIVTRPVISSSKLAAKYVDEIKKLALFLGISDAK
CCHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
LENGSLRADINISVRPENQVEFNPKVEIKNINSISNIQKAAELEIQEQIYAYENGIKVKQ
ECCCCEEEEEEEEECCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VTKKFNDVIARNQILRLKTDAIDYKYFPEPNLPYIELKPNFVDKIQVPLTPSQIEKMLKK
HHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCEEEECCCCHHEEECCCCHHHHHHHHHH
EGVSTFYINQILNNFNYTYLNNSNPDNSLSVKLFFSEIVPIINKLGFENLAIEFDFFGKI
CCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEHHHHHHH
IQKHFNKELSNSEIRKIIEIKQTNKQFNLNDILSKIKEKKLSEAAIKNLLKEVVSEEFAQ
HHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKNRQDKSKLFKFLIGKLMQKTKGNAEPIKVNQVLKEWLDSFN
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure
MNNKYLVTIGIEIHLELNTKAKMFSNSPNLRGEANQFFNLFDLAYLGTLPKINKEAVKKA
CCCCEEEEEEEEEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
IILAKALKMQISPILAFDRKNYFYPDLPKGFQISQQFHPIGKNGILKVGDFNVEIERIHL
HHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCEEEEEEEE
EEDTAKQIHKDNQTFFDYNRCGVPLIEIVTRPVISSSKLAAKYVDEIKKLALFLGISDAK
CCHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
LENGSLRADINISVRPENQVEFNPKVEIKNINSISNIQKAAELEIQEQIYAYENGIKVKQ
ECCCCEEEEEEEEECCCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VTKKFNDVIARNQILRLKTDAIDYKYFPEPNLPYIELKPNFVDKIQVPLTPSQIEKMLKK
HHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCEEEECCCCHHEEECCCCHHHHHHHHHH
EGVSTFYINQILNNFNYTYLNNSNPDNSLSVKLFFSEIVPIINKLGFENLAIEFDFFGKI
CCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEHHHHHHH
IQKHFNKELSNSEIRKIIEIKQTNKQFNLNDILSKIKEKKLSEAAIKNLLKEVVSEEFAQ
HHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEKNRQDKSKLFKFLIGKLMQKTKGNAEPIKVNQVLKEWLDSFN
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]