| Definition | Burkholderia pseudomallei K96243 chromosome 2, complete sequence. |
|---|---|
| Accession | NC_006351 |
| Length | 3,173,005 |
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The map label for this gene is rafA [H]
Identifier: 53723097
GI number: 53723097
Start: 2816328
End: 2818499
Strand: Reverse
Name: rafA [H]
Synonym: BPSS2081
Alternate gene names: 53723097
Gene position: 2818499-2816328 (Counterclockwise)
Preceding gene: 53723098
Following gene: 53723096
Centisome position: 88.83
GC content: 57.73
Gene sequence:
>2172_bases ATGTTCGACACAAAACTTATTCGATTGACTGGTTCAAATCTCAGCATCGCACTAGAGAAATCGGCCGATATGCCGCCTGT ATGGCGCTATTTTGGCGCAAAATTAGAACCGCACGCCATCGCCTGGAGCGCAGACGACGTGCGACCGCACCCTCCGACGT TACTCGACGGCGATCCGCCCTTCACGGCATTCTCGACACACGGATTCGGCTGGTTCCATCAACCGGCACTCGTCGGCAGC CGGCCTGGAGACGTTGGCGATATTGACTGGGCACAGAGCTTTCGCCTTGATGAGGTAAGCACAAACGATCACTCGGTAAC AATGAAGTTGAGCGACGAACAGGCCGAGTTGGGTGCAATCCTTAGTTACACAATCGATCCTGCGTCGGACGTCATAACGA TGTGGGCTGAATTAGAGAACCGCGGCGCGACTCCATTCCGTGTAGATTGGCTAGCCGCCGCCACTGTACCGCTGCCGCCC GACGCAAATCGGGCACTGAGCTTCTCGGGGCGCTGGACGCTGGAGTTCCAGGAAAATCGTGAAATTCTCGGTTCTGCGAC GTGGAGGCGCGACAACCGACGCGGGCGCACTTCTCACGACTCGTTTCCGGGAGTCATGGTCGGCTCATCGCTAGCGGACA ATGTGGGCACCGTATACGGCGCGCATTTGGGATGGAGTGGAAACCACACCATCATCATCGAACCGATGACCGATGGTCGC CGGCAACTGCAATTGGGGGAATGGTTGGCACCGGGCGAAGTCGTGCTTGCACCGGACCAACGCTATCGCACACCATGCGC CTTTCTTTCATACAGCGAGCAGGGCCTGAATGGCTTGAGCGCGCAGTTCCACCGCTTCGTTCGGGCGAAGGTACTGCGCT GGCCACGGCAACGCATGCAACCTCGGCCGGTGATCCTCAATACTTGGGAAGCCGTATATTGCGATCACGATCTCGAGGAT CTCAAGGAACTGGCGACTGCGGGGGCGCAGATCGGCATCGAACGTTTCGTTCTCGACGACGGCTGGTTCCATTGCCGCGA CAACGATCGCGCCGGACTAGGCGACTGGTGGCCCGATGCGAACAAGTACTCTCGCGGGCTCACACCACTCATCCATCACG TTCGAGATTTGGACATGGACTTTGGCCTATGGATCGAACCCGAAATGGTCAATCCGGACAGCGAATTGTACCGCGCCCGC CCGGAGTGGACGCTTCGGCTCGACGGTCGGCCACTCGTGACAGGTCGCAATCAGCTCGTACTGGACCTCGCGGAACCAGA TGTGCGCGAATATCTCTTCACGCAAATCAGTGCTTTATTGCGCGACAATGCGATTTCCTATCTCAAATGGGATATGAACC GAGATCTCGCCACGGCTGGCCATCGTGGCATGCCCGCTTACAGGCGTCAGACCCAGGCGTTTTATGCGTTGCTCGATCGG TTGCGCGCCGCGTTTCCTGCTGTGGAGATCGAGAGTTGCGCCTCCGGGGGCGGACGAGCCGATTACGGTGTGTTGGCTCG CATGCACCGTCTCTGGACGAGCGACTGCAATGACGCGCTCACGCGCATCGGAATTCAGCGCGGCTTTCTGCGTTTCTTTC CTCCCGAGCTCATGGGTGCACATATCGGGGCCGATCGTTCCCACACGACCGGACGCCGGCACACTCTTGCTTTCAGAGCC GCAGTCGCACTTTTCGGTCACCTTGGCGTTGAAGTCGACCCGAGAGCAATGCCCGCCGACGAGATCGAAGCCCTCATGCG CTGGATTGCAATATACAAGGAATGGCGGCCCGTTCTACATGCGGGAACCCTTCACCAGGGGTGTATCAGCGAGGCGCTCT CTTGGACTCAAGCTGTCTCCGATGACGGAAGTGCGTCGCTTTTGGCGATCTATCGACTACGCGAGGACAACGCGCGATAC GCCACACCACTCAAGATCAAGGGACTGAGATGCGACATCGCCTATCGAGTACAAACGCTCTACGCACCTGTCGCACCACA TTTCGTGAAGCAAACGCAAATGTTCAAGATGATGCAGAACGAAGGCGTGGTGATTTCGGGCGCAAGTTTGCTGGAGTTAG GGATGCCAATTCCTCCCATGCCGCCCGAATCCGCCGTTGTGATTTCGCTAAAACCCGCCTCGGAGTTATCCAAACCGCTT GATCTGTATTAA
Upstream 100 bases:
>100_bases GGTTCGCTTGTCGCGGCTCTCCCGCCCGTGCTTATTTTTTTCCTCCGGCAACGATACTTCATTGCAGGCCTCACGATGGG GGCCACCAAGGGCTAGACGT
Downstream 100 bases:
>100_bases ATGAATGGCTGCATCACTCCAACACCGGGAGAAAGAAGAAACCATGTTCAAGTTTTCACGCGACAGGCTTGAGCCTTGGG CGTGCGAGTGCGTCACTTGA
Product: alpha-galactosidase
Products: NA
Alternate protein names: Melibiase [H]
Number of amino acids: Translated: 723; Mature: 723
Protein sequence:
>723_residues MFDTKLIRLTGSNLSIALEKSADMPPVWRYFGAKLEPHAIAWSADDVRPHPPTLLDGDPPFTAFSTHGFGWFHQPALVGS RPGDVGDIDWAQSFRLDEVSTNDHSVTMKLSDEQAELGAILSYTIDPASDVITMWAELENRGATPFRVDWLAAATVPLPP DANRALSFSGRWTLEFQENREILGSATWRRDNRRGRTSHDSFPGVMVGSSLADNVGTVYGAHLGWSGNHTIIIEPMTDGR RQLQLGEWLAPGEVVLAPDQRYRTPCAFLSYSEQGLNGLSAQFHRFVRAKVLRWPRQRMQPRPVILNTWEAVYCDHDLED LKELATAGAQIGIERFVLDDGWFHCRDNDRAGLGDWWPDANKYSRGLTPLIHHVRDLDMDFGLWIEPEMVNPDSELYRAR PEWTLRLDGRPLVTGRNQLVLDLAEPDVREYLFTQISALLRDNAISYLKWDMNRDLATAGHRGMPAYRRQTQAFYALLDR LRAAFPAVEIESCASGGGRADYGVLARMHRLWTSDCNDALTRIGIQRGFLRFFPPELMGAHIGADRSHTTGRRHTLAFRA AVALFGHLGVEVDPRAMPADEIEALMRWIAIYKEWRPVLHAGTLHQGCISEALSWTQAVSDDGSASLLAIYRLREDNARY ATPLKIKGLRCDIAYRVQTLYAPVAPHFVKQTQMFKMMQNEGVVISGASLLELGMPIPPMPPESAVVISLKPASELSKPL DLY
Sequences:
>Translated_723_residues MFDTKLIRLTGSNLSIALEKSADMPPVWRYFGAKLEPHAIAWSADDVRPHPPTLLDGDPPFTAFSTHGFGWFHQPALVGS RPGDVGDIDWAQSFRLDEVSTNDHSVTMKLSDEQAELGAILSYTIDPASDVITMWAELENRGATPFRVDWLAAATVPLPP DANRALSFSGRWTLEFQENREILGSATWRRDNRRGRTSHDSFPGVMVGSSLADNVGTVYGAHLGWSGNHTIIIEPMTDGR RQLQLGEWLAPGEVVLAPDQRYRTPCAFLSYSEQGLNGLSAQFHRFVRAKVLRWPRQRMQPRPVILNTWEAVYCDHDLED LKELATAGAQIGIERFVLDDGWFHCRDNDRAGLGDWWPDANKYSRGLTPLIHHVRDLDMDFGLWIEPEMVNPDSELYRAR PEWTLRLDGRPLVTGRNQLVLDLAEPDVREYLFTQISALLRDNAISYLKWDMNRDLATAGHRGMPAYRRQTQAFYALLDR LRAAFPAVEIESCASGGGRADYGVLARMHRLWTSDCNDALTRIGIQRGFLRFFPPELMGAHIGADRSHTTGRRHTLAFRA AVALFGHLGVEVDPRAMPADEIEALMRWIAIYKEWRPVLHAGTLHQGCISEALSWTQAVSDDGSASLLAIYRLREDNARY ATPLKIKGLRCDIAYRVQTLYAPVAPHFVKQTQMFKMMQNEGVVISGASLLELGMPIPPMPPESAVVISLKPASELSKPL DLY >Mature_723_residues MFDTKLIRLTGSNLSIALEKSADMPPVWRYFGAKLEPHAIAWSADDVRPHPPTLLDGDPPFTAFSTHGFGWFHQPALVGS RPGDVGDIDWAQSFRLDEVSTNDHSVTMKLSDEQAELGAILSYTIDPASDVITMWAELENRGATPFRVDWLAAATVPLPP DANRALSFSGRWTLEFQENREILGSATWRRDNRRGRTSHDSFPGVMVGSSLADNVGTVYGAHLGWSGNHTIIIEPMTDGR RQLQLGEWLAPGEVVLAPDQRYRTPCAFLSYSEQGLNGLSAQFHRFVRAKVLRWPRQRMQPRPVILNTWEAVYCDHDLED LKELATAGAQIGIERFVLDDGWFHCRDNDRAGLGDWWPDANKYSRGLTPLIHHVRDLDMDFGLWIEPEMVNPDSELYRAR PEWTLRLDGRPLVTGRNQLVLDLAEPDVREYLFTQISALLRDNAISYLKWDMNRDLATAGHRGMPAYRRQTQAFYALLDR LRAAFPAVEIESCASGGGRADYGVLARMHRLWTSDCNDALTRIGIQRGFLRFFPPELMGAHIGADRSHTTGRRHTLAFRA AVALFGHLGVEVDPRAMPADEIEALMRWIAIYKEWRPVLHAGTLHQGCISEALSWTQAVSDDGSASLLAIYRLREDNARY ATPLKIKGLRCDIAYRVQTLYAPVAPHFVKQTQMFKMMQNEGVVISGASLLELGMPIPPMPPESAVVISLKPASELSKPL DLY
Specific function: Unknown
COG id: COG3345
COG function: function code G; Alpha-galactosidase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 36 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013785 - InterPro: IPR002252 - InterPro: IPR017853 - InterPro: IPR000111 [H]
Pfam domain/function: PF02065 Melibiase [H]
EC number: =3.2.1.22 [H]
Molecular weight: Translated: 81196; Mature: 81196
Theoretical pI: Translated: 6.37; Mature: 6.37
Prosite motif: PS00512 ALPHA_GALACTOSIDASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.7 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFDTKLIRLTGSNLSIALEKSADMPPVWRYFGAKLEPHAIAWSADDVRPHPPTLLDGDPP CCCCEEEEEECCCEEEEEECCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC FTAFSTHGFGWFHQPALVGSRPGDVGDIDWAQSFRLDEVSTNDHSVTMKLSDEQAELGAI CCEEECCCCCEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCHHHHCEE LSYTIDPASDVITMWAELENRGATPFRVDWLAAATVPLPPDANRALSFSGRWTLEFQENR EEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCEEEECCEEEEEECCCC EILGSATWRRDNRRGRTSHDSFPGVMVGSSLADNVGTVYGAHLGWSGNHTIIIEPMTDGR HHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHHCCHHEEEEEECCCCCCEEEEEECCCCC RQLQLGEWLAPGEVVLAPDQRYRTPCAFLSYSEQGLNGLSAQFHRFVRAKVLRWPRQRMQ HHHHHCCCCCCCCEEECCCCCCCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHCHHHHCC PRPVILNTWEAVYCDHDLEDLKELATAGAQIGIERFVLDDGWFHCRDNDRAGLGDWWPDA CCCEEEECCEEEEECCCHHHHHHHHHCCHHHCEEEEEEECCEEEECCCCCCCCCCCCCCC NKYSRGLTPLIHHVRDLDMDFGLWIEPEMVNPDSELYRARPEWTLRLDGRPLVTGRNQLV HHHHCCCHHHHHHHHHCCCCCCCEECCCCCCCCHHHHCCCCCEEEEECCEEEEECCCEEE LDLAEPDVREYLFTQISALLRDNAISYLKWDMNRDLATAGHRGMPAYRRQTQAFYALLDR EECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHH LRAAFPAVEIESCASGGGRADYGVLARMHRLWTSDCNDALTRIGIQRGFLRFFPPELMGA HHHHCCCEEHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHH HIGADRSHTTGRRHTLAFRAAVALFGHLGVEVDPRAMPADEIEALMRWIAIYKEWRPVLH HCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH AGTLHQGCISEALSWTQAVSDDGSASLLAIYRLREDNARYATPLKIKGLRCDIAYRVQTL CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEECCEEEECEEEEHHHHHHHH YAPVAPHFVKQTQMFKMMQNEGVVISGASLLELGMPIPPMPPESAVVISLKPASELSKPL HHCCCHHHHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCCCCEEEEEECCHHHCCCCC DLY CCC >Mature Secondary Structure MFDTKLIRLTGSNLSIALEKSADMPPVWRYFGAKLEPHAIAWSADDVRPHPPTLLDGDPP CCCCEEEEEECCCEEEEEECCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC FTAFSTHGFGWFHQPALVGSRPGDVGDIDWAQSFRLDEVSTNDHSVTMKLSDEQAELGAI CCEEECCCCCEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCHHHHCEE LSYTIDPASDVITMWAELENRGATPFRVDWLAAATVPLPPDANRALSFSGRWTLEFQENR EEEECCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCEEEECCEEEEEECCCC EILGSATWRRDNRRGRTSHDSFPGVMVGSSLADNVGTVYGAHLGWSGNHTIIIEPMTDGR HHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHHCCHHEEEEEECCCCCCEEEEEECCCCC RQLQLGEWLAPGEVVLAPDQRYRTPCAFLSYSEQGLNGLSAQFHRFVRAKVLRWPRQRMQ HHHHHCCCCCCCCEEECCCCCCCCCCCEEECCHHCCCHHHHHHHHHHHHHHHHCHHHHCC PRPVILNTWEAVYCDHDLEDLKELATAGAQIGIERFVLDDGWFHCRDNDRAGLGDWWPDA CCCEEEECCEEEEECCCHHHHHHHHHCCHHHCEEEEEEECCEEEECCCCCCCCCCCCCCC NKYSRGLTPLIHHVRDLDMDFGLWIEPEMVNPDSELYRARPEWTLRLDGRPLVTGRNQLV HHHHCCCHHHHHHHHHCCCCCCCEECCCCCCCCHHHHCCCCCEEEEECCEEEEECCCEEE LDLAEPDVREYLFTQISALLRDNAISYLKWDMNRDLATAGHRGMPAYRRQTQAFYALLDR EECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHH LRAAFPAVEIESCASGGGRADYGVLARMHRLWTSDCNDALTRIGIQRGFLRFFPPELMGA HHHHCCCEEHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHH HIGADRSHTTGRRHTLAFRAAVALFGHLGVEVDPRAMPADEIEALMRWIAIYKEWRPVLH HCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH AGTLHQGCISEALSWTQAVSDDGSASLLAIYRLREDNARYATPLKIKGLRCDIAYRVQTL CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEECCEEEECEEEEHHHHHHHH YAPVAPHFVKQTQMFKMMQNEGVVISGASLLELGMPIPPMPPESAVVISLKPASELSKPL HHCCCHHHHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCCCCEEEEEECCHHHCCCCC DLY CCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2556373; 2180920; 786627 [H]