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The map label for this gene is gadA [C]

Identifier: 53723043

GI number: 53723043

Start: 2736052

End: 2737575

Strand: Reverse

Name: gadA [C]

Synonym: BPSS2025

Alternate gene names: 53723043

Gene position: 2737575-2736052 (Counterclockwise)

Preceding gene: 53723044

Following gene: 53723042

Centisome position: 86.28

GC content: 66.47

Gene sequence:

>1524_bases
ATGCGCGTCGCCTGCTTCGTCGATTTCATGCAACCGCACCCCGGCAACATTCGAACCCACATAGGCGTGCCGACCGACGT
CGGCACGGCGAAGGAGAACGTCATGGATCTGGAGGAAGGCATCAGGCGGCTGTATCCGTATGCGGAGGAATTCGGCGTGA
TGCGCGGCTTCCCGGAACAAGGAACGCCGCGCGACGAACTGCTCGCGCAGTTGCGCTCGATGGCCGAGCGCGAGGATCGC
AACTGGGAGAGCGGGCATAGCTCGGGCGCGATGTATTCCGGCGATCGCGACCATCATGCATGGCTGAACGAAGCGTATTC
GGTCTTCAGTCACGTCAACGCGCTGCGGCGCGACATGTGCCCGAGCATGAACCGGATGGAAAGCGAGATCGTCGGCATGA
CGGTTGCGATGCTGCACGGCGATGCGGTGGCCGCGCACCACCCCGGGCAGCGCGCGTGCGGCATGATCACGCTCGGCGGT
ACCGAGAGCATCCTGGGCGCGACGCTTGCGTATCGCGAGAAAGCGCGCGCCGAGCGCGGCATCGAGCGGCCGCGGATGAT
CTGGCCGGCGTCCGCGCATCCGGTGTTTCGCAAGGCCGCGCATCTGTTCGGCTTCGACGTGACCGTCGCGCCGATCGATC
CCGTCACGATGCAGGTCGATGCCGATTTCGTGCGCGACGCGGTGGACGCGAATACGGTGATGCTCGTGGGCTCGGCATGC
AATTATCCGTACGGCACGATCGATCCGATCGGCGCGCTGTCGGCGATCGCCGTCGAAAAGGACGTGTGGCTGCACGTCGA
CGGGTGCCTCGGCGGCTGGATGCTGCCGTGGGGCGAAGCGCTGGGCTATCCGGATATTCCGGCATTCGATTTTCGTCTGC
CGGGCGTCACGTCGATCTCGGCCGATACGCACAAGTTCGGCTACGGCCCGAAGGGCGGCTCGGTGCTCGCGTGGCGTGAC
GCGAGCTTTCGGCGCCATCAGTATTTTCTGATGACCGATTGGGTGGGCGGCGTCTATGGCTCGCCGGGATTGACGGGCAG
CCGCTCGGGCGGCCTGATCGCGGCGACGTGGGCGGCGCTGCGCGGCCTCGGGCGCGAAGGCTACCTCGCGCGGGCGAAGG
CGATCTTCGAGACGGCCTTCGACATGCAGGCCGCGGTGCGCGCGATTCCGGAGCTGCGCGTGCTCGGCAAGCCGACGTTC
TGCTTCGCGTTCACCTCGGACGCGTTCGACATCTATCACGTGAACGATTTCATGCGGCAGCGCGGCTGGCGTCTCAATGG
CCTGCGGCGCCCCGATGCGTTGCAGATGTGCGTGACGGGGCCGCAGACGCAGCCGGGCGTCGCCGAGCAGTTCCGGTGCG
ATCTCGGCGCGGCGGCGGATTACGCGCGCGCTCACGCGCAGGAGCGCCCGAAGACGAGCGGCGTCTATGCGAGCGATGCG
GCCGGCGTCGATCTGAGCGACGATGCGCGAACGAGGGCGTTCTTCACGCAGGTGATCGATCTGTTCACGGATTGCCCGCT
TTAA

Upstream 100 bases:

>100_bases
GCCGCGTATTCTTCAACGAATCGAATTCGCACCGAAAAATACCGGTAGCAACGAACGCCGATCAATCATCGTCTGATATA
TTTTTTCGGCTTCGACGACG

Downstream 100 bases:

>100_bases
GCGGCGGCGCGCCGGCTGTTGCGTCACGCGTCGCATTCGCGGTGCGGCGCGACGCGGCGGCCGCCGTCGGTTTCCGTCGA
AGAATGCATCGCCGATGCAT

Product: decarboxylase

Products: NA

Alternate protein names: SP-lyase; SPL; Sphingosine-1-phosphate aldolase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MRVACFVDFMQPHPGNIRTHIGVPTDVGTAKENVMDLEEGIRRLYPYAEEFGVMRGFPEQGTPRDELLAQLRSMAEREDR
NWESGHSSGAMYSGDRDHHAWLNEAYSVFSHVNALRRDMCPSMNRMESEIVGMTVAMLHGDAVAAHHPGQRACGMITLGG
TESILGATLAYREKARAERGIERPRMIWPASAHPVFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSAC
NYPYGTIDPIGALSAIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSISADTHKFGYGPKGGSVLAWRD
ASFRRHQYFLMTDWVGGVYGSPGLTGSRSGGLIAATWAALRGLGREGYLARAKAIFETAFDMQAAVRAIPELRVLGKPTF
CFAFTSDAFDIYHVNDFMRQRGWRLNGLRRPDALQMCVTGPQTQPGVAEQFRCDLGAAADYARAHAQERPKTSGVYASDA
AGVDLSDDARTRAFFTQVIDLFTDCPL

Sequences:

>Translated_507_residues
MRVACFVDFMQPHPGNIRTHIGVPTDVGTAKENVMDLEEGIRRLYPYAEEFGVMRGFPEQGTPRDELLAQLRSMAEREDR
NWESGHSSGAMYSGDRDHHAWLNEAYSVFSHVNALRRDMCPSMNRMESEIVGMTVAMLHGDAVAAHHPGQRACGMITLGG
TESILGATLAYREKARAERGIERPRMIWPASAHPVFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSAC
NYPYGTIDPIGALSAIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSISADTHKFGYGPKGGSVLAWRD
ASFRRHQYFLMTDWVGGVYGSPGLTGSRSGGLIAATWAALRGLGREGYLARAKAIFETAFDMQAAVRAIPELRVLGKPTF
CFAFTSDAFDIYHVNDFMRQRGWRLNGLRRPDALQMCVTGPQTQPGVAEQFRCDLGAAADYARAHAQERPKTSGVYASDA
AGVDLSDDARTRAFFTQVIDLFTDCPL
>Mature_507_residues
MRVACFVDFMQPHPGNIRTHIGVPTDVGTAKENVMDLEEGIRRLYPYAEEFGVMRGFPEQGTPRDELLAQLRSMAEREDR
NWESGHSSGAMYSGDRDHHAWLNEAYSVFSHVNALRRDMCPSMNRMESEIVGMTVAMLHGDAVAAHHPGQRACGMITLGG
TESILGATLAYREKARAERGIERPRMIWPASAHPVFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSAC
NYPYGTIDPIGALSAIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSISADTHKFGYGPKGGSVLAWRD
ASFRRHQYFLMTDWVGGVYGSPGLTGSRSGGLIAATWAALRGLGREGYLARAKAIFETAFDMQAAVRAIPELRVLGKPTF
CFAFTSDAFDIYHVNDFMRQRGWRLNGLRRPDALQMCVTGPQTQPGVAEQFRCDLGAAADYARAHAQERPKTSGVYASDA
AGVDLSDDARTRAFFTQVIDLFTDCPL

Specific function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Possibly implicated in influencing the macrophage autophagy pathway [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=446, Percent_Identity=38.1165919282511, Blast_Score=310, Evalue=3e-84,
Organism=Escherichia coli, GI1789934, Length=392, Percent_Identity=24.7448979591837, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787769, Length=392, Percent_Identity=24.7448979591837, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557272, Length=443, Percent_Identity=36.7945823927765, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17543922, Length=416, Percent_Identity=35.5769230769231, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI25148342, Length=403, Percent_Identity=35.4838709677419, Blast_Score=250, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6320500, Length=455, Percent_Identity=35.1648351648352, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6323905, Length=275, Percent_Identity=22.9090909090909, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355963, Length=454, Percent_Identity=39.647577092511, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24654344, Length=454, Percent_Identity=39.647577092511, Blast_Score=330, Evalue=9e-91,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.2.27 [H]

Molecular weight: Translated: 55695; Mature: 55695

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVACFVDFMQPHPGNIRTHIGVPTDVGTAKENVMDLEEGIRRLYPYAEEFGVMRGFPEQ
CEEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
GTPRDELLAQLRSMAEREDRNWESGHSSGAMYSGDRDHHAWLNEAYSVFSHVNALRRDMC
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
PSMNRMESEIVGMTVAMLHGDAVAAHHPGQRACGMITLGGTESILGATLAYREKARAERG
CCHHHHHHHHHHHEEEEEECCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHC
IERPRMIWPASAHPVFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSAC
CCCCCEECCCCCCHHHHHHHHHHCCEEEEECCCCEEEEECHHHHHHHCCCCEEEEEECCC
NYPYGTIDPIGALSAIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSIS
CCCCCCCCCHHHHHHHEEECCEEEEECCCCCCCCCCCHHHCCCCCCCCCEEECCCCCCCC
ADTHKFGYGPKGGSVLAWRDASFRRHQYFLMTDWVGGVYGSPGLTGSRSGGLIAATWAAL
CCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHH
RGLGREGYLARAKAIFETAFDMQAAVRAIPELRVLGKPTFCFAFTSDAFDIYHVNDFMRQ
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCCEEEEEHHHHHHH
RGWRLNGLRRPDALQMCVTGPQTQPGVAEQFRCDLGAAADYARAHAQERPKTSGVYASDA
CCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEECCC
AGVDLSDDARTRAFFTQVIDLFTDCPL
CCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVACFVDFMQPHPGNIRTHIGVPTDVGTAKENVMDLEEGIRRLYPYAEEFGVMRGFPEQ
CEEEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
GTPRDELLAQLRSMAEREDRNWESGHSSGAMYSGDRDHHAWLNEAYSVFSHVNALRRDMC
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
PSMNRMESEIVGMTVAMLHGDAVAAHHPGQRACGMITLGGTESILGATLAYREKARAERG
CCHHHHHHHHHHHEEEEEECCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHC
IERPRMIWPASAHPVFRKAAHLFGFDVTVAPIDPVTMQVDADFVRDAVDANTVMLVGSAC
CCCCCEECCCCCCHHHHHHHHHHCCEEEEECCCCEEEEECHHHHHHHCCCCEEEEEECCC
NYPYGTIDPIGALSAIAVEKDVWLHVDGCLGGWMLPWGEALGYPDIPAFDFRLPGVTSIS
CCCCCCCCCHHHHHHHEEECCEEEEECCCCCCCCCCCHHHCCCCCCCCCEEECCCCCCCC
ADTHKFGYGPKGGSVLAWRDASFRRHQYFLMTDWVGGVYGSPGLTGSRSGGLIAATWAAL
CCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHH
RGLGREGYLARAKAIFETAFDMQAAVRAIPELRVLGKPTFCFAFTSDAFDIYHVNDFMRQ
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEECCCEEEEEHHHHHHH
RGWRLNGLRRPDALQMCVTGPQTQPGVAEQFRCDLGAAADYARAHAQERPKTSGVYASDA
CCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEECCC
AGVDLSDDARTRAFFTQVIDLFTDCPL
CCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA