Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 53722275

GI number: 53722275

Start: 1696611

End: 1697945

Strand: Reverse

Name: shiA [H]

Synonym: BPSS1252

Alternate gene names: 53722275

Gene position: 1697945-1696611 (Counterclockwise)

Preceding gene: 53722277

Following gene: 53722274

Centisome position: 53.51

GC content: 69.44

Gene sequence:

>1335_bases
GTGAGGAGAAACGCATTGACCCGGGGCATCCCACCGAGCCCGCCGGCGCGCCAGCCGCGCCGCGCGGCCGGCGCCGCGTT
CGTCGGCACGATGATCGAGTGGTACGACTTCTACATCTACGCGAACGCGGCCGCGCTCGTCTTCGGCGAGCTGTTCTTCC
CGTCGCACGATCCGTTCACGAGCACGATGGCGTCGTTCGCGACGTTCGCGGTCGGCTTCTTCGCGCGCCCGCTCGGCGGG
CTCTTCTTCGGCCATCTCGGCGACCGGATCGGCCGCAAGAAGGCGCTGATGGCGACGCTCGCGCTGATGGGCGCGGCGAC
GGTCTGCGTCGGTCTGCTGCCGACCTACGAACGCGTCGGCCTGCTCGCGCCCGTGCTGCTCGTGCTGCTGCGCATCGTTC
AGGGCATCGCGGTGGGCGGCGAGTGGGGCGGCGCGGTGCTGATGGCGGGCGAGCATGCGCCGTATGGGCGGCGCACGTTC
TTCGCTTCGTTCGCGCAGTTGGGCAGCCCGGCCGGGCTGATCCTGTCGCTCGTCGCGTTTCGCGCGGTCGCGTCGATGGA
GCACGGCGCGTTCATGTCCTGGGGCTGGCGGCTGCCGTTTCTCGCGAGCATCGCGCTGCTCGCGGTCGGCGTGTTCGTGC
GGCTGAAGGTTGACGAATCGCCCGAGTTCGCGCATGAGAAGGCGATGTCGCGCACCGTCGCGCGGCCGCTCGCCGAGGTG
CTGCGCACGTCGCGCGCGATGCTGCTGTTCTGCCTGTGCGCGAACACGATCGGCATCGCGGGGCTGTATTTCACGAACAC
GTTCATGATCGCGTTCACGACGCAGCATGTGGGCGTGACGAAATCGCTGATCCTCGATTGCCTGTTCATCGTCGCGATCA
TCCAGTTCGTGACGCAGCCGATCGCCGCGTGGCTCGGCGGCAAGCTCGGCGACGCACGCTTTCTCAAGCTCGCCGCGCTG
TTCGCGATGGCCTCGCCCTATCCGATGTTCGCGCTCGTGCAGACGGGGCGGCTCGCGCCGATGGTGATCGGCATCGCGAT
CGCGACCGTGTTTCTCGCCGGTTTCTACTCGGTGATCGCGGGCTTCGTGAGCGGCGCGTTCCCGACGCGCGTGCGCTATT
CGGCGATCTCGATCTCGTACCAGATGTGCGGCGCGATCGCGGGCGGGCTGACGCCGCTCGTCGGCACGTGGCTCGCGCAC
CGGTTCGCCGGGCAGTGGTGGCCGCTCGCGGTGTTCTATACGTGCCTCGCGGCGCTCTCGCTCGGCGGCGTGATCGCGCT
CGACGCGCGCCGCCATCGGCGGGCCGACGTCGCCGACGCCGTGCTCACGCGCTGA

Upstream 100 bases:

>100_bases
ATTCTTAAGATAATAACTTAGAATTTGACCGATTAATCCCGGTGCGCGATAAAACGCCCATTTGCCGAATCCGGACGATT
CGGCCGGGGCGGACATTCGA

Downstream 100 bases:

>100_bases
TTCAAGAAAAGGAGGGAGATCTCGGTGTGGGAAATTGACGGTAGGCGGCTCTGGAACAGCCTGACGGAACTCGCGCGAAT
CGGCGGCACCGCGCGCGGCG

Product: inner membrane transport protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRRNALTRGIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALVFGELFFPSHDPFTSTMASFATFAVGFFARPLGG
LFFGHLGDRIGRKKALMATLALMGAATVCVGLLPTYERVGLLAPVLLVLLRIVQGIAVGGEWGGAVLMAGEHAPYGRRTF
FASFAQLGSPAGLILSLVAFRAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEKAMSRTVARPLAEV
LRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDCLFIVAIIQFVTQPIAAWLGGKLGDARFLKLAAL
FAMASPYPMFALVQTGRLAPMVIGIAIATVFLAGFYSVIAGFVSGAFPTRVRYSAISISYQMCGAIAGGLTPLVGTWLAH
RFAGQWWPLAVFYTCLAALSLGGVIALDARRHRRADVADAVLTR

Sequences:

>Translated_444_residues
MRRNALTRGIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALVFGELFFPSHDPFTSTMASFATFAVGFFARPLGG
LFFGHLGDRIGRKKALMATLALMGAATVCVGLLPTYERVGLLAPVLLVLLRIVQGIAVGGEWGGAVLMAGEHAPYGRRTF
FASFAQLGSPAGLILSLVAFRAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEKAMSRTVARPLAEV
LRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDCLFIVAIIQFVTQPIAAWLGGKLGDARFLKLAAL
FAMASPYPMFALVQTGRLAPMVIGIAIATVFLAGFYSVIAGFVSGAFPTRVRYSAISISYQMCGAIAGGLTPLVGTWLAH
RFAGQWWPLAVFYTCLAALSLGGVIALDARRHRRADVADAVLTR
>Mature_444_residues
MRRNALTRGIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALVFGELFFPSHDPFTSTMASFATFAVGFFARPLGG
LFFGHLGDRIGRKKALMATLALMGAATVCVGLLPTYERVGLLAPVLLVLLRIVQGIAVGGEWGGAVLMAGEHAPYGRRTF
FASFAQLGSPAGLILSLVAFRAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEKAMSRTVARPLAEV
LRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDCLFIVAIIQFVTQPIAAWLGGKLGDARFLKLAAL
FAMASPYPMFALVQTGRLAPMVIGIAIATVFLAGFYSVIAGFVSGAFPTRVRYSAISISYQMCGAIAGGLTPLVGTWLAH
RFAGQWWPLAVFYTCLAALSLGGVIALDARRHRRADVADAVLTR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=36.1702127659575, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1790550, Length=393, Percent_Identity=31.8066157760814, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=33.7995337995338, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1788942, Length=388, Percent_Identity=30.1546391752577, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI87082231, Length=439, Percent_Identity=22.0956719817768, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082404, Length=392, Percent_Identity=20.9183673469388, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47663; Mature: 47663

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNALTRGIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALVFGELFFPSHDPFT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
STMASFATFAVGFFARPLGGLFFGHLGDRIGRKKALMATLALMGAATVCVGLLPTYERVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LLAPVLLVLLRIVQGIAVGGEWGGAVLMAGEHAPYGRRTFFASFAQLGSPAGLILSLVAF
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
RAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEKAMSRTVARPLAEV
HHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
LRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDCLFIVAIIQFVTQP
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHCHHHHHHHHHHHHHHHHHHHHHH
IAAWLGGKLGDARFLKLAALFAMASPYPMFALVQTGRLAPMVIGIAIATVFLAGFYSVIA
HHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GFVSGAFPTRVRYSAISISYQMCGAIAGGLTPLVGTWLAHRFAGQWWPLAVFYTCLAALS
HHHCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LGGVIALDARRHRRADVADAVLTR
HCCEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRNALTRGIPPSPPARQPRRAAGAAFVGTMIEWYDFYIYANAAALVFGELFFPSHDPFT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
STMASFATFAVGFFARPLGGLFFGHLGDRIGRKKALMATLALMGAATVCVGLLPTYERVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LLAPVLLVLLRIVQGIAVGGEWGGAVLMAGEHAPYGRRTFFASFAQLGSPAGLILSLVAF
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
RAVASMEHGAFMSWGWRLPFLASIALLAVGVFVRLKVDESPEFAHEKAMSRTVARPLAEV
HHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
LRTSRAMLLFCLCANTIGIAGLYFTNTFMIAFTTQHVGVTKSLILDCLFIVAIIQFVTQP
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHCHHHHHHHHHHHHHHHHHHHHHH
IAAWLGGKLGDARFLKLAALFAMASPYPMFALVQTGRLAPMVIGIAIATVFLAGFYSVIA
HHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GFVSGAFPTRVRYSAISISYQMCGAIAGGLTPLVGTWLAHRFAGQWWPLAVFYTCLAALS
HHHCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LGGVIALDARRHRRADVADAVLTR
HCCEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]