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The map label for this gene is amiD [H]

Identifier: 53719485

GI number: 53719485

Start: 2225273

End: 2226172

Strand: Reverse

Name: amiD [H]

Synonym: BPSL1872

Alternate gene names: 53719485

Gene position: 2226172-2225273 (Counterclockwise)

Preceding gene: 53719486

Following gene: 53719484

Centisome position: 54.64

GC content: 70.56

Gene sequence:

>900_bases
ATGAAATTTCCCCTGCGCCATACGCTTCTCGCGAGCTTCAGCATGCTCGCCGCCGCCTGTACGACGCCCACGCTCGTCAA
TCGCGGCGATTACTACGCCGACACGAGCCTTCCCGCGCGCAGCGTCGATTCGCGCATCCGCTTCCTCGTCATGCACTACA
CCGAGATCGGCGAAGCGCCGTCGCTGCGCGTGTTGACGCAGGAGAACGTCAGCGCCCATTACGTCGTGCCCGACGCGCCG
CGCACCGAGCGGGGCGCGCCCGTCGTCTATCAGCTCGTGCCGGAAAGCGAGCGCGCATGGCACGCGGGCGTGAGCGCGTG
GCAGGGCGCGACCGAGCTGAACGGCGTGTCGATCGGCATCGAGAACGTCAACCGCGGCCCGATCGATACGCCGCACGGCC
GCATGTGGGCGCCGTATCCGCCCGCGCAGGTCGACGCGATCGTTCGGCTGGCGAAGGATATCGTCGCCCGCTATCGGATT
CCGCCGACGCGCGTCGTCGGCCACAGCGACATCGCGCCGCAGCGCAAGATCGATCCGGGCCCGCTGTTTCCTTGGCGCGC
GCTCGCGAAGGCGGGCGTCGGCGCGTGGCCGGACGACGAGACGGTGGCCGCGCGCCTCGCGGGCCGCCCGCCGAAGTCGC
TCGTCGACGTGCGCGAGCTGCAGCTGAAGCTCGCGCGCTACGGTTACGACGTCGCGACGGACGGCGTGCTCGACGACCGC
ACGCGGCGCGTGTTCTCCGCGTTCCAGATGCATTTCCGGCCGACCGATTACGCGGGCAACCCGGATGCGGAGACCGACGC
GATCGCGCAGGCGCTGCTCGACAAGTACATGCCGTCGCAGCCGGCGCCCGAGCGCGATGCGGCGGCGCTCGACGCGGCGA
GCGGCGTGGCCGCCCAATGA

Upstream 100 bases:

>100_bases
CACGAAACGCGCAGGAATGCGAGCGACGAAGCGTCTAGAATGAGGCGCTCGTGCCGGGACGCCCGTCCCGGCGACCGTCG
ATTCACCGAGCGCCGTCATC

Downstream 100 bases:

>100_bases
GCGTGCCGGGTACGCGCGCGGCGGGCGGCATGTCGGCGGCATTTGCGTCGGCGTCGGAATCGCACGATCGGAAAGGCGGG
CGATAAGAAAGTCGGACGCT

Product: putative N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKFPLRHTLLASFSMLAAACTTPTLVNRGDYYADTSLPARSVDSRIRFLVMHYTEIGEAPSLRVLTQENVSAHYVVPDAP
RTERGAPVVYQLVPESERAWHAGVSAWQGATELNGVSIGIENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYRI
PPTRVVGHSDIAPQRKIDPGPLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
TRRVFSAFQMHFRPTDYAGNPDAETDAIAQALLDKYMPSQPAPERDAAALDAASGVAAQ

Sequences:

>Translated_299_residues
MKFPLRHTLLASFSMLAAACTTPTLVNRGDYYADTSLPARSVDSRIRFLVMHYTEIGEAPSLRVLTQENVSAHYVVPDAP
RTERGAPVVYQLVPESERAWHAGVSAWQGATELNGVSIGIENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYRI
PPTRVVGHSDIAPQRKIDPGPLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
TRRVFSAFQMHFRPTDYAGNPDAETDAIAQALLDKYMPSQPAPERDAAALDAASGVAAQ
>Mature_299_residues
MKFPLRHTLLASFSMLAAACTTPTLVNRGDYYADTSLPARSVDSRIRFLVMHYTEIGEAPSLRVLTQENVSAHYVVPDAP
RTERGAPVVYQLVPESERAWHAGVSAWQGATELNGVSIGIENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYRI
PPTRVVGHSDIAPQRKIDPGPLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
TRRVFSAFQMHFRPTDYAGNPDAETDAIAQALLDKYMPSQPAPERDAAALDAASGVAAQ

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=252, Percent_Identity=54.7619047619048, Blast_Score=261, Evalue=4e-71,
Organism=Escherichia coli, GI1786300, Length=126, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 32684; Mature: 32684

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPLRHTLLASFSMLAAACTTPTLVNRGDYYADTSLPARSVDSRIRFLVMHYTEIGEAP
CCCCHHHHHHHHHHHHHHHHCCCHHCCCCCEEECCCCCHHHHHHHHHHHHEEHHHHCCCC
SLRVLTQENVSAHYVVPDAPRTERGAPVVYQLVPESERAWHAGVSAWQGATELNGVSIGI
CEEEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEC
ENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYRIPPTRVVGHSDIAPQRKIDPG
CCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCCCC
PLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
CCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TRRVFSAFQMHFRPTDYAGNPDAETDAIAQALLDKYMPSQPAPERDAAALDAASGVAAQ
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFPLRHTLLASFSMLAAACTTPTLVNRGDYYADTSLPARSVDSRIRFLVMHYTEIGEAP
CCCCHHHHHHHHHHHHHHHHCCCHHCCCCCEEECCCCCHHHHHHHHHHHHEEHHHHCCCC
SLRVLTQENVSAHYVVPDAPRTERGAPVVYQLVPESERAWHAGVSAWQGATELNGVSIGI
CEEEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEC
ENVNRGPIDTPHGRMWAPYPPAQVDAIVRLAKDIVARYRIPPTRVVGHSDIAPQRKIDPG
CCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCCCC
PLFPWRALAKAGVGAWPDDETVAARLAGRPPKSLVDVRELQLKLARYGYDVATDGVLDDR
CCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TRRVFSAFQMHFRPTDYAGNPDAETDAIAQALLDKYMPSQPAPERDAAALDAASGVAAQ
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]