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The map label for this gene is rlmB [H]

Identifier: 53719486

GI number: 53719486

Start: 2226546

End: 2227289

Strand: Reverse

Name: rlmB [H]

Synonym: BPSL1873

Alternate gene names: 53719486

Gene position: 2227289-2226546 (Counterclockwise)

Preceding gene: 53719487

Following gene: 53719485

Centisome position: 54.66

GC content: 70.16

Gene sequence:

>744_bases
ATGTCACGTCTGAAGGTTCTCTACGGTTTTCATGCGGTGACCGCACGCATGCGGCACGATGCGTCGACGGTCGCGGAGGT
GTTCTACGATGCGACGCGCCGCGATCGCCGGATGCAGGATTTCCTGCAGGCGGCGAAGGAGGCGGGGGTGCGCCTCATCG
CCGCCGACGAGACGCGGCTCTGGGGGCTCGCGCATACCGAGCGCCACCAGGGCGTCGTCGCGCGCGTCGAGGATCTGCCG
CTCGCGCAGAACCTCGCCGAGCTGCTCGACGGGATCGCCGGGCCCGCGCTCTTGCTCGTGCTCGACGGCGTGACCGATCC
GCACAACCTCGGCGCATGCCTGCGCGTGGCCGACGCGGCGGGCGCGCACGCGATCATCGCGCCGCGCGACCGCGCGGCCG
GCCTGAACGCGACCGCGGCGAAGGTCGCGAGCGGCGCGGCCGATACGGTGCCGTACATCACGGTGACGAACCTCGCGCGT
GCGCTGCGCGAGCTGAAGGACGCGGGCGTATGGGTGATCGGCACGTCGGACGACGCGACGGCGAGCGTGTACGACACCAA
GCTCGACGGCCCCGTCGCGCTCGTGATGGGTGCGGAAGGCGAGGGAATGCGGCGCCTCACGCGTGATACGTGCGACGAAG
TGATGTTCATTCCGATGGCGGGCAGCGTCGAGAGCCTGAACGTGTCGGTCGCGAGCGGCGTGTGCCTGTACGAGGCGGTG
CGCCAGCGAGGCGCGAAGAAGTAA

Upstream 100 bases:

>100_bases
CGAAACGCCGCGCGCCCCGCCAGGGCGCGCGGCGCTTTCCGTTTTCCATGCGGCGCGTGCGATGCGCGGCCGCGCGTCTT
GTTCTAAGGTTGTTCCAGCC

Downstream 100 bases:

>100_bases
GCGCGCGGCGCATGCGCTCGTTCGCCGCGGGCTTTGCGGGGGCGGTGCGCGGGGCGGCGTTCGCGATGCCCGGCGGCCGC
TCGGCGGCGGCGTTCGCGGT

Product: putative tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVIGTSDDATASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAV
RQRGAKK

Sequences:

>Translated_247_residues
MSRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLP
LAQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLAR
ALRELKDAGVWVIGTSDDATASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAV
RQRGAKK
>Mature_246_residues
SRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDLPL
AQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAAGAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARA
LRELKDAGVWVIGTSDDATASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAVR
QRGAKK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=265, Percent_Identity=27.1698113207547, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=52.6970954356847, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=34.8684210526316, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788935, Length=241, Percent_Identity=29.045643153527, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17555908, Length=254, Percent_Identity=25.5905511811024, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=162, Percent_Identity=31.4814814814815, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26289; Mature: 26158

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVIGTSDDAT
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCE
ASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAV
EEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEHHHCCHHHHHHH
RQRGAKK
HHHCCCC
>Mature Secondary Structure 
SRLKVLYGFHAVTARMRHDASTVAEVFYDATRRDRRMQDFLQAAKEAGVRLIAADETRL
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDLPLAQNLAELLDGIAGPALLLVLDGVTDPHNLGACLRVADAA
EECCHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GAHAIIAPRDRAAGLNATAAKVASGAADTVPYITVTNLARALRELKDAGVWVIGTSDDAT
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCE
ASVYDTKLDGPVALVMGAEGEGMRRLTRDTCDEVMFIPMAGSVESLNVSVASGVCLYEAV
EEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEHHHCCHHHHHHH
RQRGAKK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]