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The map label for this gene is yieH [H]

Identifier: 53718322

GI number: 53718322

Start: 773388

End: 774074

Strand: Reverse

Name: yieH [H]

Synonym: BPSL0679

Alternate gene names: 53718322

Gene position: 774074-773388 (Counterclockwise)

Preceding gene: 53718323

Following gene: 53718318

Centisome position: 19.0

GC content: 68.41

Gene sequence:

>687_bases
ATGCTCGACCACCTCATTTGCGACTGCGACGGCGTGCTCGTCGACAGCGAAGTGATCGCCGATCGCGTGCTGTTCGACAC
GCTGTCGGCCACGTTCCCCGCCATCGATTTCACGGCCGACGCGAAAGCCGCGTTCGGCCAGCAGACCTCGCGCTTCCTCG
CGGATCTCGAAGCGCGCCACGGCATCGCGATGCCGGCGAATTTCCTCGATACGATCGAGCACAACATCGAGCTCGGGCTC
GCGCAATGGCTCGCGCCGATCGCGGGCGTGCGCGACGCGCTGACAAGAATCGCGTTGCCCGCGGCGGTCGTGTCGAACAG
CCGGCTCGCGCGCGTGCGCAACTCGCTCAAGCGCGCAGCGCTCACCGACGTGTTCGGCGAGCGCGTGTTCAGCGCCGAGC
AGGTCGCGCGGCCGAAGCCCTACCCCGATGTCTACCTGCATGCGGCGCGCACGCTCGGCGTCGAGCCGGCGCGCTGCGTG
GTCGTCGAGGACAGCGTATCGGGGCTGAACGCCGCGCGCGCGGCCGGCATGAAGACGATCGCGTTCGTCGGCGCGAGCCA
CATTCCCGGCGGCTATGAAGACGCGCTGCGCAAGATGGGCATCACGCGAATCATGCGTTCGATGGGCGAGTTGCCCGCGC
TCGTCGAGGCGGGCATGCGCGGCGAATTCGGCAGCCAGCAGTCCTAG

Upstream 100 bases:

>100_bases
TCATGGCGGCGCGTGTCGCCGCGCGCAGGCGCGGTCGCGCCCGCGGCGGGCAAAGCACGTATCATGGCGCTTCGTCGCCT
CCCCCTTTTTTGTTGTCCTT

Downstream 100 bases:

>100_bases
CCGGGCGAACTCCCCTCTCGTGCGCGGCTTGCGCGCCGCGCACGAGCCGCCGCCGGATCCGAACCGCTCAGGCGAGGTCC
GCGCAGCATGCGTCGCGTGC

Product: putative hydrolase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MLDHLICDCDGVLVDSEVIADRVLFDTLSATFPAIDFTADAKAAFGQQTSRFLADLEARHGIAMPANFLDTIEHNIELGL
AQWLAPIAGVRDALTRIALPAAVVSNSRLARVRNSLKRAALTDVFGERVFSAEQVARPKPYPDVYLHAARTLGVEPARCV
VVEDSVSGLNAARAAGMKTIAFVGASHIPGGYEDALRKMGITRIMRSMGELPALVEAGMRGEFGSQQS

Sequences:

>Translated_228_residues
MLDHLICDCDGVLVDSEVIADRVLFDTLSATFPAIDFTADAKAAFGQQTSRFLADLEARHGIAMPANFLDTIEHNIELGL
AQWLAPIAGVRDALTRIALPAAVVSNSRLARVRNSLKRAALTDVFGERVFSAEQVARPKPYPDVYLHAARTLGVEPARCV
VVEDSVSGLNAARAAGMKTIAFVGASHIPGGYEDALRKMGITRIMRSMGELPALVEAGMRGEFGSQQS
>Mature_228_residues
MLDHLICDCDGVLVDSEVIADRVLFDTLSATFPAIDFTADAKAAFGQQTSRFLADLEARHGIAMPANFLDTIEHNIELGL
AQWLAPIAGVRDALTRIALPAAVVSNSRLARVRNSLKRAALTDVFGERVFSAEQVARPKPYPDVYLHAARTLGVEPARCV
VVEDSVSGLNAARAAGMKTIAFVGASHIPGGYEDALRKMGITRIMRSMGELPALVEAGMRGEFGSQQS

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=188, Percent_Identity=29.2553191489362, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1788021, Length=181, Percent_Identity=32.0441988950276, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129151, Length=224, Percent_Identity=29.4642857142857, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24449; Mature: 24449

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDHLICDCDGVLVDSEVIADRVLFDTLSATFPAIDFTADAKAAFGQQTSRFLADLEARH
CCCCEEECCCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GIAMPANFLDTIEHNIELGLAQWLAPIAGVRDALTRIALPAAVVSNSRLARVRNSLKRAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTDVFGERVFSAEQVARPKPYPDVYLHAARTLGVEPARCVVVEDSVSGLNAARAAGMKTI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCHHHH
AFVGASHIPGGYEDALRKMGITRIMRSMGELPALVEAGMRGEFGSQQS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLDHLICDCDGVLVDSEVIADRVLFDTLSATFPAIDFTADAKAAFGQQTSRFLADLEARH
CCCCEEECCCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GIAMPANFLDTIEHNIELGLAQWLAPIAGVRDALTRIALPAAVVSNSRLARVRNSLKRAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTDVFGERVFSAEQVARPKPYPDVYLHAARTLGVEPARCVVVEDSVSGLNAARAAGMKTI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCHHHH
AFVGASHIPGGYEDALRKMGITRIMRSMGELPALVEAGMRGEFGSQQS
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]