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The map label for this gene is dmlR [H]

Identifier: 53718318

GI number: 53718318

Start: 769122

End: 770054

Strand: Reverse

Name: dmlR [H]

Synonym: BPSL0675

Alternate gene names: 53718318

Gene position: 770054-769122 (Counterclockwise)

Preceding gene: 53718322

Following gene: 53718317

Centisome position: 18.9

GC content: 69.02

Gene sequence:

>933_bases
ATGGATCGACTGGGCGACATCCGCTTGTTCGTCGAGGCGGCGGAGCTCGGCAGCCTGTCGGCCGCCGGGCGCAAGCTGAA
CCTCACGCCCGCCGCCGCGAGCGCGCGGCTCGCGAAGCTGGAGGCGCAGGTGTCGACGCGCCTGTTCGAGCGTTCGACAC
GGCGGCTGCGGCTCACCGACGAAGGCCGCCTGTACCTGAACTGCTGCCGGCAGGCGCTGCAGGCGCTCGACGATGCGGAC
GCGATGCTGCAGGAAGGGCGCAACGTCGTGAGCGGCAAGGTGCGGCTGTCGTCGACGGCGGATTTCGGGCGCCATCAACT
GCTCGACTGGCTCGACGAATTCAATACGCGCCATCCGGAGGTGACTTTCGCCGTGACCGCGTCGGATTCGTCGTCGAATC
TCTGGCAGGACGAGATCGATCTCGCGATCCGCTTCGCCGCGCCGCCCGACGGCGCGCTGATCGCGCGGCGGCTCGCCGCG
AACCGGCGCGTGCTGTGCGCGTCGCCCGCGTTCGTCGACCGATACGGCATGCCGAAGGACCCGCTCGACCTGGCCCGCTT
TCCGTGCAACGTGATCACGGTCGCGTCGGGGCCGCTCAACGTCTGGCGCTTCACGCGCGGCGACGACACGCAGACCTATA
CGGTGCCGCTCGCGAACGCGCACGAGACGAACGACGGCGGCCTCACGCGCGAATGGACGATCCGCGGCTACGGAATCGCG
CTGAAATCGATCTGGGACATCGCGGCCGACGTCCGCGCGGGCCGGCTCAGGGTGCTGTTGCCCGACTGGCGGCACCACGA
CGCGCCGCTGCACGCGATTTATCACAGCAAGCGCTACATGGCGCCGCGCGTGCGGGTGCTGCTCGATTTTCTCGTCGAGC
GCTTCGCGCGGGAGGAAAAGGCACTGGAGGATCTGCTGAACGCGTGCCGGTGA

Upstream 100 bases:

>100_bases
CGCATCAAGAAATCTGACGAGCGCGCATTGTCCCCAATCCGAAGTTTTTTGATAATTGGCGTAGCATTTGAACCATTTTC
AAAAGTTTTCTTAAAATCGC

Downstream 100 bases:

>100_bases
CGACGGGCGCGATAGACGCCCACGGTTTCGCCGCGCATCGGCCCACGGGTTCGGTTCGTCGCGTCGCGGCCCCGCGAAAT
CCGGCTTGCGCGAATGCCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAQVSTRLFERSTRRLRLTDEGRLYLNCCRQALQALDDAD
AMLQEGRNVVSGKVRLSSTADFGRHQLLDWLDEFNTRHPEVTFAVTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCASPAFVDRYGMPKDPLDLARFPCNVITVASGPLNVWRFTRGDDTQTYTVPLANAHETNDGGLTREWTIRGYGIA
LKSIWDIAADVRAGRLRVLLPDWRHHDAPLHAIYHSKRYMAPRVRVLLDFLVERFAREEKALEDLLNACR

Sequences:

>Translated_310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAQVSTRLFERSTRRLRLTDEGRLYLNCCRQALQALDDAD
AMLQEGRNVVSGKVRLSSTADFGRHQLLDWLDEFNTRHPEVTFAVTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCASPAFVDRYGMPKDPLDLARFPCNVITVASGPLNVWRFTRGDDTQTYTVPLANAHETNDGGLTREWTIRGYGIA
LKSIWDIAADVRAGRLRVLLPDWRHHDAPLHAIYHSKRYMAPRVRVLLDFLVERFAREEKALEDLLNACR
>Mature_310_residues
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAQVSTRLFERSTRRLRLTDEGRLYLNCCRQALQALDDAD
AMLQEGRNVVSGKVRLSSTADFGRHQLLDWLDEFNTRHPEVTFAVTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAA
NRRVLCASPAFVDRYGMPKDPLDLARFPCNVITVASGPLNVWRFTRGDDTQTYTVPLANAHETNDGGLTREWTIRGYGIA
LKSIWDIAADVRAGRLRVLLPDWRHHDAPLHAIYHSKRYMAPRVRVLLDFLVERFAREEKALEDLLNACR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=32.8813559322034, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=32.1799307958478, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789639, Length=305, Percent_Identity=26.8852459016393, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=29.0657439446367, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=28.8079470198675, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=26.027397260274, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786448, Length=188, Percent_Identity=29.2553191489362, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788706, Length=184, Percent_Identity=27.7173913043478, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789173, Length=260, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=28.6713286713287, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=173, Percent_Identity=24.8554913294798, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787806, Length=136, Percent_Identity=30.1470588235294, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34805; Mature: 34805

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAQVSTRLFERSTRRLRLTD
CCCCCCEEEEEEHHHCCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECC
EGRLYLNCCRQALQALDDADAMLQEGRNVVSGKVRLSSTADFGRHQLLDWLDEFNTRHPE
CCCHHHHHHHHHHHHHCHHHHHHHCCCCEEECEEEECCCCCHHHHHHHHHHHHHCCCCCE
VTFAVTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAANRRVLCASPAFVDRYGMPKD
EEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCCCC
PLDLARFPCNVITVASGPLNVWRFTRGDDTQTYTVPLANAHETNDGGLTREWTIRGYGIA
CHHHHHCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEEHHHH
LKSIWDIAADVRAGRLRVLLPDWRHHDAPLHAIYHSKRYMAPRVRVLLDFLVERFAREEK
HHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
ALEDLLNACR
HHHHHHHHCC
>Mature Secondary Structure
MDRLGDIRLFVEAAELGSLSAAGRKLNLTPAAASARLAKLEAQVSTRLFERSTRRLRLTD
CCCCCCEEEEEEHHHCCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECC
EGRLYLNCCRQALQALDDADAMLQEGRNVVSGKVRLSSTADFGRHQLLDWLDEFNTRHPE
CCCHHHHHHHHHHHHHCHHHHHHHCCCCEEECEEEECCCCCHHHHHHHHHHHHHCCCCCE
VTFAVTASDSSSNLWQDEIDLAIRFAAPPDGALIARRLAANRRVLCASPAFVDRYGMPKD
EEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHHCCCCCC
PLDLARFPCNVITVASGPLNVWRFTRGDDTQTYTVPLANAHETNDGGLTREWTIRGYGIA
CHHHHHCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEEHHHH
LKSIWDIAADVRAGRLRVLLPDWRHHDAPLHAIYHSKRYMAPRVRVLLDFLVERFAREEK
HHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
ALEDLLNACR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]