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The map label for this gene is mbtM [H]

Identifier: 53718132

GI number: 53718132

Start: 534524

End: 536290

Strand: Reverse

Name: mbtM [H]

Synonym: BPSL0493

Alternate gene names: 53718132

Gene position: 536290-534524 (Counterclockwise)

Preceding gene: 53718139

Following gene: 53718131

Centisome position: 13.16

GC content: 70.8

Gene sequence:

>1767_bases
ATGCGTGATTCCTCGCCGCTGATCGAAAGGCTGTTGGCTCCGCTCGCAGAGGGTCGGAACTGCCGCGCGCACGGCGTCGG
CACGCTCGACGAAAGCGGCATCGAGCGCGTGCTGTCCTACCGGCAACTGAGCGAAGCGGGCCTGCGCATGAGCGCCGCGC
TGAACGCGCTCGGCGCGCGCGCGGGCGATATCGCCCTCGTCGCGCTGCCCGCGACGGGCGACTATCTCGCGCTCCTCGTC
GGCTGCGTGCTGTCGGGCGTCGTGCCGTGCACGGTGCCGCCGCCGCTCAAGCGCGCGAGCGGCTCGGGCCCGCAGGTGCT
CGAAGTCGCGTGCCGGCTCTATCGCCCGCGGTTCGTGTTCACGGCGTCCGCGCACGTCGCCGAGCTCGAGGCGCAACTGC
GCGACACGGGCGCGCGCGTGATCGACGTCGGCATCCTGCATGCGGCGTCGACGGGCGCCGAGCCGCGGCTCGCGCTGCGC
GGCGCCGGCGACACGCATCACATCCAGCTGACGTCCGGCTCCACCTCGCATCCGAAGGCGGCCGTCATCAGCCATCGCAA
CGTCGCGGCCAACATCGCCGGCATCGCGAACGCGTGCGGCTACAGCAAGCACGCCGCCGACAACACCGTGATCTGGCTGC
CGCTGCATCACGACATGGGCCTCGTCAGCCTGCTGCTGCATCTGTATTACAGGACGTCGCTGCGGCTGATGCCTTCGATG
AGCTTCGTGCGCAATCCGCTCGGCTGGCTCAGGCGCATCGCGCACGCGCGCTCGACCATCGCCGTCGCGCCCACCTTCGC
GCTGCGCTACTGCGTGCGCCGCTTCAACGCGGCGACGATGGACGGCGCGGACTTCAGCCATCTGCGCACGTTCCTCGTCG
GCGCGGAGCGGGTCGATCGCGCGACGCTGTCCGATTTCGCGTCGACGTTCGCGCCGTACGGCTTTCACGCATCGGCGCTG
CAGCCGTGCTACGGAATGGCGGAGGCAACGCTCGCCGTCACGCTGCACCGCTCGGTCGACACGCCGAAAGCGCCGGCGTT
CACGCACGTGATCGCCGATCGCATCGACACCGCGACGCTCGCGCGGCACCGCCGCGCCGCCCCCGCGCCCGATGGCCACA
CGGGCGCGACCGACACGCTGCTCGCCGTCGGCGCGCCGCTCGACGACATGGCCTACGAGATCCGCGACGCGCAGGGCCGG
CTCGCCGCCGAGCGCGAAGCGGGCGAAATCTTCATTCGCGGCGCATCGGTGATGCAGGGCTATCTGCCGAGCGCCGGCGC
GCCCGCGCCGCAGCCGTTCGACGCGCACGGCTGGTTCGCGACGGGCGACATCGGCTACGTGAGCGGCGGCCAGCTCTTCA
TCCTCGGGCGCAAGAAGGAAATCATCATCATCCGCGGCACGAACTACTTTCCGCACGAGATCGAGGAGACGATCGACAGC
CATCCGGCCGTCTACAAAGGCGCCTGCATCGCGGTGGGCATTCACGACGAGACGCAGGGTACCGAGAACCTCGTCGTCCT
CGTCGAGGCGCACGCGAATCACGTGAGCCGCGATTCTCGCGCCGAGTTGCAGGCGCGCATGCAGCAGCGGCTCGGCTACT
GCGCGCAGCGCATCGCGTTCGTCGAGCCGGGCGCGCTGCCGCGCACGACGAGCGGCAAACCGCAACGGCTGAAGGCGCGC
GCGATGTTCGTGAGCGGCGAGCTGCGCGAGATCGCGCTGCCGCCGTCGCGCCGCGCCGATGCCGATCGCGTCGCGCCGTA
CCCGTGA

Upstream 100 bases:

>100_bases
ACTGTATGGAAATTGTCAGGCAAGAACGCTTATTGGACGAACAATCCTGATTAATTATTGCGGATCGAGTTTTAGCGACT
TTTTAATAAAGGGGCAGTCA

Downstream 100 bases:

>100_bases
GCGCCTCGCCGCTTCGCCCGACACCGCCCGCGCGCGTTGCGCGCACGACGCGCCTCACGCCCCCCACCGCCCGATTGCCG
ATGATGCCCGATACCGCCCT

Product: putative AMP-binding enzyme

Products: NA

Alternate protein names: Fatty acyl-[acyl-carrier-protein] synthetase; Fatty acyl-ACP synthetase; Mycobactin synthetase protein M [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MRDSSPLIERLLAPLAEGRNCRAHGVGTLDESGIERVLSYRQLSEAGLRMSAALNALGARAGDIALVALPATGDYLALLV
GCVLSGVVPCTVPPPLKRASGSGPQVLEVACRLYRPRFVFTASAHVAELEAQLRDTGARVIDVGILHAASTGAEPRLALR
GAGDTHHIQLTSGSTSHPKAAVISHRNVAANIAGIANACGYSKHAADNTVIWLPLHHDMGLVSLLLHLYYRTSLRLMPSM
SFVRNPLGWLRRIAHARSTIAVAPTFALRYCVRRFNAATMDGADFSHLRTFLVGAERVDRATLSDFASTFAPYGFHASAL
QPCYGMAEATLAVTLHRSVDTPKAPAFTHVIADRIDTATLARHRRAAPAPDGHTGATDTLLAVGAPLDDMAYEIRDAQGR
LAAEREAGEIFIRGASVMQGYLPSAGAPAPQPFDAHGWFATGDIGYVSGGQLFILGRKKEIIIIRGTNYFPHEIEETIDS
HPAVYKGACIAVGIHDETQGTENLVVLVEAHANHVSRDSRAELQARMQQRLGYCAQRIAFVEPGALPRTTSGKPQRLKAR
AMFVSGELREIALPPSRRADADRVAPYP

Sequences:

>Translated_588_residues
MRDSSPLIERLLAPLAEGRNCRAHGVGTLDESGIERVLSYRQLSEAGLRMSAALNALGARAGDIALVALPATGDYLALLV
GCVLSGVVPCTVPPPLKRASGSGPQVLEVACRLYRPRFVFTASAHVAELEAQLRDTGARVIDVGILHAASTGAEPRLALR
GAGDTHHIQLTSGSTSHPKAAVISHRNVAANIAGIANACGYSKHAADNTVIWLPLHHDMGLVSLLLHLYYRTSLRLMPSM
SFVRNPLGWLRRIAHARSTIAVAPTFALRYCVRRFNAATMDGADFSHLRTFLVGAERVDRATLSDFASTFAPYGFHASAL
QPCYGMAEATLAVTLHRSVDTPKAPAFTHVIADRIDTATLARHRRAAPAPDGHTGATDTLLAVGAPLDDMAYEIRDAQGR
LAAEREAGEIFIRGASVMQGYLPSAGAPAPQPFDAHGWFATGDIGYVSGGQLFILGRKKEIIIIRGTNYFPHEIEETIDS
HPAVYKGACIAVGIHDETQGTENLVVLVEAHANHVSRDSRAELQARMQQRLGYCAQRIAFVEPGALPRTTSGKPQRLKAR
AMFVSGELREIALPPSRRADADRVAPYP
>Mature_588_residues
MRDSSPLIERLLAPLAEGRNCRAHGVGTLDESGIERVLSYRQLSEAGLRMSAALNALGARAGDIALVALPATGDYLALLV
GCVLSGVVPCTVPPPLKRASGSGPQVLEVACRLYRPRFVFTASAHVAELEAQLRDTGARVIDVGILHAASTGAEPRLALR
GAGDTHHIQLTSGSTSHPKAAVISHRNVAANIAGIANACGYSKHAADNTVIWLPLHHDMGLVSLLLHLYYRTSLRLMPSM
SFVRNPLGWLRRIAHARSTIAVAPTFALRYCVRRFNAATMDGADFSHLRTFLVGAERVDRATLSDFASTFAPYGFHASAL
QPCYGMAEATLAVTLHRSVDTPKAPAFTHVIADRIDTATLARHRRAAPAPDGHTGATDTLLAVGAPLDDMAYEIRDAQGR
LAAEREAGEIFIRGASVMQGYLPSAGAPAPQPFDAHGWFATGDIGYVSGGQLFILGRKKEIIIIRGTNYFPHEIEETIDS
HPAVYKGACIAVGIHDETQGTENLVVLVEAHANHVSRDSRAELQARMQQRLGYCAQRIAFVEPGALPRTTSGKPQRLKAR
AMFVSGELREIALPPSRRADADRVAPYP

Specific function: Activates lipidic moieties required for mycobactin biosynthesis. Converts medium- to long-chain aliphatic fatty acids into acyl adenylate, which is further transferred on to the phosphopantetheine arm of the carrier protein MbtL [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=564, Percent_Identity=22.8723404255319, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI145693145, Length=156, Percent_Identity=32.6923076923077, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17559526, Length=592, Percent_Identity=22.1283783783784, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=423, Percent_Identity=23.1678486997636, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=611, Percent_Identity=21.9312602291326, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=407, Percent_Identity=24.0786240786241, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356947, Length=570, Percent_Identity=23.3333333333333, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24581924, Length=403, Percent_Identity=23.5732009925558, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24653035, Length=189, Percent_Identity=31.2169312169312, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648257, Length=507, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648260, Length=183, Percent_Identity=30.0546448087432, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161076582, Length=195, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.20 [H]

Molecular weight: Translated: 63105; Mature: 63105

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDSSPLIERLLAPLAEGRNCRAHGVGTLDESGIERVLSYRQLSEAGLRMSAALNALGAR
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AGDIALVALPATGDYLALLVGCVLSGVVPCTVPPPLKRASGSGPQVLEVACRLYRPRFVF
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCEEEE
TASAHVAELEAQLRDTGARVIDVGILHAASTGAEPRLALRGAGDTHHIQLTSGSTSHPKA
ECCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCCCCCE
AVISHRNVAANIAGIANACGYSKHAADNTVIWLPLHHDMGLVSLLLHLYYRTSLRLMPSM
EEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCH
SFVRNPLGWLRRIAHARSTIAVAPTFALRYCVRRFNAATMDGADFSHLRTFLVGAERVDR
HHHHCHHHHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
ATLSDFASTFAPYGFHASALQPCYGMAEATLAVTLHRSVDTPKAPAFTHVIADRIDTATL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
ARHRRAAPAPDGHTGATDTLLAVGAPLDDMAYEIRDAQGRLAAEREAGEIFIRGASVMQG
HHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCHHHCCCCCEEEEEEHHHHHC
YLPSAGAPAPQPFDAHGWFATGDIGYVSGGQLFILGRKKEIIIIRGTNYFPHEIEETIDS
CCCCCCCCCCCCCCCCCCEEECCCEEECCCEEEEEECCCEEEEEECCCCCCHHHHHHHCC
HPAVYKGACIAVGIHDETQGTENLVVLVEAHANHVSRDSRAELQARMQQRLGYCAQRIAF
CCCEECCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VEPGALPRTTSGKPQRLKARAMFVSGELREIALPPSRRADADRVAPYP
CCCCCCCCCCCCCCHHHEEEEEEECCCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRDSSPLIERLLAPLAEGRNCRAHGVGTLDESGIERVLSYRQLSEAGLRMSAALNALGAR
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AGDIALVALPATGDYLALLVGCVLSGVVPCTVPPPLKRASGSGPQVLEVACRLYRPRFVF
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCEEEE
TASAHVAELEAQLRDTGARVIDVGILHAASTGAEPRLALRGAGDTHHIQLTSGSTSHPKA
ECCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCCCCCE
AVISHRNVAANIAGIANACGYSKHAADNTVIWLPLHHDMGLVSLLLHLYYRTSLRLMPSM
EEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCH
SFVRNPLGWLRRIAHARSTIAVAPTFALRYCVRRFNAATMDGADFSHLRTFLVGAERVDR
HHHHCHHHHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
ATLSDFASTFAPYGFHASALQPCYGMAEATLAVTLHRSVDTPKAPAFTHVIADRIDTATL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
ARHRRAAPAPDGHTGATDTLLAVGAPLDDMAYEIRDAQGRLAAEREAGEIFIRGASVMQG
HHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCHHHCCCCCEEEEEEHHHHHC
YLPSAGAPAPQPFDAHGWFATGDIGYVSGGQLFILGRKKEIIIIRGTNYFPHEIEETIDS
CCCCCCCCCCCCCCCCCCEEECCCEEECCCEEEEEECCCEEEEEECCCCCCHHHHHHHCC
HPAVYKGACIAVGIHDETQGTENLVVLVEAHANHVSRDSRAELQARMQQRLGYCAQRIAF
CCCEECCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VEPGALPRTTSGKPQRLKARAMFVSGELREIALPPSRRADADRVAPYP
CCCCCCCCCCCCCCHHHEEEEEEECCCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]