| Definition | Burkholderia pseudomallei K96243 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_006350 |
| Length | 4,074,542 |
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The map label for this gene is metH1 [H]
Identifier: 53718025
GI number: 53718025
Start: 416706
End: 419408
Strand: Reverse
Name: metH1 [H]
Synonym: BPSL0385
Alternate gene names: 53718025
Gene position: 419408-416706 (Counterclockwise)
Preceding gene: 53718026
Following gene: 53718023
Centisome position: 10.29
GC content: 65.78
Gene sequence:
>2703_bases ATGCGCCTAGCCGGCCTCGAGCCGTTCAACGTCACGTCCGGGACGCTCTTCATCAACGTCGGCGAACGCACCAACGTCAC CGGCTCGAAGGCGTTCGCGCGCATGATCCTCAACGGCCAGTTCGACGAGGCGCTCGCGGTCGCGCGCCAGCAGGTCGAGA ACGGCGCGCAGGTGATCGACGTCAACATGGACGAGGCGATGCTCGATTCGAAGGCGGCGATGGTGCGCTTCCTGAACCTG ATCGCATCCGAGCCGGACATCGCGCGCGTGCCGATCATGATCGATTCGTCGAAGTGGGACGTGATCGAGGCGGGCCTGAA GTGCGTGCAGGGCAAGGCGATCGTCAACTCGATCTCGCTGAAGGAAGGCAAGGAAGCGTTCGTGCATCACGCGAAGCTGA TCCGCCGCTACGGCGCCGCGGCCGTCGTGATGGCGTTCGACGAAGCGGGCCAGGCCGACACGCTCGCACGCAAGACCGAG ATCTGCAAGCGCTCGTACGACGTGCTCGTGAACGAAGTCGGCTTCGCGCCCGAGGACATCGTCTTCGATCCGAACATCTT CGCGGTGGCGACGGGCATCGACGAGCACAACAACTACGCGGTCGACTTCATCGAGGCGACCCGCTGGATCAAGCGCAACC TGCCGTACGCGAAGGTGAGCGGCGGCGTGTCGAACGTGTCGTTCTCGTTTCGCGGCAACGATCCCGTGCGCGAGGCGATC CACACCGTGTTCCTCTATCACGCGATCCAGGCGGGGATGGACATGGGCATCGTCAACGCGGGCCAGCTGGGCGTGTACGC GGAGCTCGACGCCGAGCTGCGCGAACGCGTCGAGGACGTGATCCTCAACCGCCGCGACGATTCGACCGACCGGCTGCTCG AGATCGCCGACAAGTTCAAGACCGGCGCCGCGAAGAAGGAAGAGAACCTCGAATGGCGCAGCCAGCCGGTCGAGAAGCGG CTCGCGCACGCGCTCGTGCACGGCATCACGAACTTCATCGTCGAGGATACCGAAGAAGCGCGCGCGAAGATCGCCGCGGC GGGCGGGCGCCCGATCAACGTGATCGAAGGCCCGCTGATGGACGGGATGAACATCGTCGGCGACCTGTTCGGCCAGGGCA AGATGTTCCTGCCGCAGGTGGTGAAATCCGCGCGCGTGATGAAACAGGCCGTCGCGCACCTGATCCCGTTCATCGAGGAA GAGAAGCGCCTGCTCGCGCAAGCGGGCGGCGACGTGCGCGCGAAGGGCAAGATCGTCATCGCGACCGTGAAGGGCGACGT GCACGACATCGGCAAGAACATCGTGTCGGTCGTGCTTCAGTGCAACAACTTCGAAGTGGTCAACATGGGCGTGATGGTGC CGTGCAACGAGATCCTCGCGAAGGCGAAGGTCGAGGGCGCGGACATCGTCGGGCTGTCCGGCCTCATCACGCCGAGCCTC GAGGAAATGGCGTACGTCGCCTCCGAGATGCAGCGCGACGACTACTTCCGCGTGAAGAAGATTCCGCTGCTGATCGGCGG CGCGACGACCTCGCGCGTGCACACGGCCGTGAAGATCGCGCCGCACTACGAGGGCCCGGTCGTCTACGTGCCGGACGCGT CGCGCTCGGTATCGGTCGCCTCGAACCTGCTGTCCGACGAAGGCGCGACGAAATACCTCGACGAGCTGAAAGCCGACTAC GAGCGCATCCGCGCGCAGCACGCGAACAAGAAGGCGCTGCCGCTCGTCACGCTCGCCGAAGCGCGCGCGAACAAGACGAA GATCGACTGGGCGCGCTACACGAGCGTCAAGCCGAAGTTCATCGGCCGGCGCATGTTCAAGAACTACGATCTGAACGAGC TCGCGAACTACATCGACTGGGGCCCGTTCTTCCAGACCTGGGACCTCGCGGGCCCGTACCCGGCGATCCTGAACGACGAG ATCGTCGGCGAATCGGCGCGCCGCGTATTCTCCGACGCGAAATCGATGCTCGCGCGCCTCATCCAGGGCCGCTGGCTCAC CGCGAACGGCGTGATCGCGCTGTTGCCGGCGAACACGGTGGGCGACGACGACATCGAGATCTACACCGACGACACGCGCG CCGAAGTGCTGCTCACGTGGCACAACCTGCGCCAGCAGAGCGTGCGCCCGGTGGTGGACGGCGTGATGCGGCCGAACCGC TCGCTCGCCGATTTCATCGCGCCGAAGGATTCGGGCGTCGCCGACTACATCGGGATGTTCGCGGTGACGGCCGGCATCGG CGTCGACGCGAAGGAAAAACAGTTCCTCGCCGATCACGACGACTACAGCGCGATCATGCTCAAGGCGCTCGCCGACCGGC TCGCGGAGGCGTTCGCCGAGGCGATGCACGCGCGCGTGCGCCGCGAGCTGTGGGGCTACGCGGCGACCGAGCAGCTCGAC AACGACGCGCTGATCGCCGAGCAGTACGCGGGCATCCGCCCGGCGCCCGGTTACCCGGCGTGCCCGGACCACCTCGTGAA ACGCGCAATGTTCGAAGCGCTTCATACAGACGAGATCGGGATGAGCGTCACCGATTCGCTCGCGATGCTGCCGGCCGCGA GCGTCTCCGGCTTCTATCTCGCGCATCCGGACAGCACGTACTTCTCGGTCGGCAAGATCGGCCAGGATCAGCTCGAGGAT TTCGCGCGCCGCACGGCGCTGTCGAAGGCGGACGCCGAGCGCGCGCTCGCGCCGCTGCTCTGA
Upstream 100 bases:
>100_bases GCCGCGCCGCTGGCCGAGCCAGTACAGCGAAGCCGCCTGACGCGCGCGGCCCTCCCCACTCCAACCCGAATACGCACGCA CCGCCATGACCGACCACACG
Downstream 100 bases:
>100_bases CGGGCGGCACGCGCGGCGACAAGGCCGCGGCGTGCGCGGCAGGTGAAAGCGGAGCGCACCCGACATCGAGCCGGCCGAAA CGGCGCGCAACCGGGGCCGC
Product: putative 5-methyltetrahydrofolate--homocysteine methyltransferase
Products: NA
Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]
Number of amino acids: Translated: 900; Mature: 900
Protein sequence:
>900_residues MRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDVNMDEAMLDSKAAMVRFLNL IASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGKEAFVHHAKLIRRYGAAAVVMAFDEAGQADTLARKTE ICKRSYDVLVNEVGFAPEDIVFDPNIFAVATGIDEHNNYAVDFIEATRWIKRNLPYAKVSGGVSNVSFSFRGNDPVREAI HTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRDDSTDRLLEIADKFKTGAAKKEENLEWRSQPVEKR LAHALVHGITNFIVEDTEEARAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEE EKRLLAQAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGLITPSL EEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASNLLSDEGATKYLDELKADY ERIRAQHANKKALPLVTLAEARANKTKIDWARYTSVKPKFIGRRMFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDE IVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVGDDDIEIYTDDTRAEVLLTWHNLRQQSVRPVVDGVMRPNR SLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLAEAFAEAMHARVRRELWGYAATEQLD NDALIAEQYAGIRPAPGYPACPDHLVKRAMFEALHTDEIGMSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLED FARRTALSKADAERALAPLL
Sequences:
>Translated_900_residues MRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDVNMDEAMLDSKAAMVRFLNL IASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGKEAFVHHAKLIRRYGAAAVVMAFDEAGQADTLARKTE ICKRSYDVLVNEVGFAPEDIVFDPNIFAVATGIDEHNNYAVDFIEATRWIKRNLPYAKVSGGVSNVSFSFRGNDPVREAI HTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRDDSTDRLLEIADKFKTGAAKKEENLEWRSQPVEKR LAHALVHGITNFIVEDTEEARAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEE EKRLLAQAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGLITPSL EEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASNLLSDEGATKYLDELKADY ERIRAQHANKKALPLVTLAEARANKTKIDWARYTSVKPKFIGRRMFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDE IVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVGDDDIEIYTDDTRAEVLLTWHNLRQQSVRPVVDGVMRPNR SLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLAEAFAEAMHARVRRELWGYAATEQLD NDALIAEQYAGIRPAPGYPACPDHLVKRAMFEALHTDEIGMSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLED FARRTALSKADAERALAPLL >Mature_900_residues MRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDVNMDEAMLDSKAAMVRFLNL IASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGKEAFVHHAKLIRRYGAAAVVMAFDEAGQADTLARKTE ICKRSYDVLVNEVGFAPEDIVFDPNIFAVATGIDEHNNYAVDFIEATRWIKRNLPYAKVSGGVSNVSFSFRGNDPVREAI HTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRDDSTDRLLEIADKFKTGAAKKEENLEWRSQPVEKR LAHALVHGITNFIVEDTEEARAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEE EKRLLAQAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGLITPSL EEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASNLLSDEGATKYLDELKADY ERIRAQHANKKALPLVTLAEARANKTKIDWARYTSVKPKFIGRRMFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDE IVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVGDDDIEIYTDDTRAEVLLTWHNLRQQSVRPVVDGVMRPNR SLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLAEAFAEAMHARVRRELWGYAATEQLD NDALIAEQYAGIRPAPGYPACPDHLVKRAMFEALHTDEIGMSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLED FARRTALSKADAERALAPLL
Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]
COG id: COG1410
COG function: function code E; Methionine synthase I, cobalamin-binding domain
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pterin-binding domain [H]
Homologues:
Organism=Homo sapiens, GI169790923, Length=918, Percent_Identity=54.0305010893246, Blast_Score=968, Evalue=0.0, Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=62.5, Blast_Score=1110, Evalue=0.0, Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.0570175438597, Blast_Score=948, Evalue=0.0,
Paralogues:
None
Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006158 - InterPro: IPR011005 - InterPro: IPR003759 - InterPro: IPR011822 - InterPro: IPR000489 - InterPro: IPR003726 - InterPro: IPR004223 [H]
Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]
EC number: =2.1.1.13 [H]
Molecular weight: Translated: 98822; Mature: 98822
Theoretical pI: Translated: 5.51; Mature: 5.51
Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVID CCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEE VNMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISL ECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCH KEGKEAFVHHAKLIRRYGAAAVVMAFDEAGQADTLARKTEICKRSYDVLVNEVGFAPEDI HHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCE VFDPNIFAVATGIDEHNNYAVDFIEATRWIKRNLPYAKVSGGVSNVSFSFRGNDPVREAI EECCCEEEEEECCCCCCCEEEHHHHHHHHHHHCCCCCEECCCCCEEEEEECCCCHHHHHH HTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRDDSTDRLLEIADKFK HHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH TGAAKKEENLEWRSQPVEKRLAHALVHGITNFIVEDTEEARAKIAAAGGRPINVIEGPLM CCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCC DGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAQAGGDVRAKGKIVI CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEE ATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGLITPSL EEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEECCCCCCCCCH EEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVA HHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCCHHHHH SNLLSDEGATKYLDELKADYERIRAQHANKKALPLVTLAEARANKTKIDWARYTSVKPKF HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCHHHHCCCCHHH IGRRMFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKSMLARL HHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHH IQGRWLTANGVIALLPANTVGDDDIEIYTDDTRAEVLLTWHNLRQQSVRPVVDGVMRPNR HCCCEEECCCEEEEEECCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC SLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLAEAFAE CHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH AMHARVRRELWGYAATEQLDNDALIAEQYAGIRPAPGYPACPDHLVKRAMFEALHTDEIG HHHHHHHHHHHCCHHHHHCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC MSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLEDFARRTALSKADAERALAPLL CCHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC >Mature Secondary Structure MRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVID CCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEE VNMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISL ECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCH KEGKEAFVHHAKLIRRYGAAAVVMAFDEAGQADTLARKTEICKRSYDVLVNEVGFAPEDI HHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCE VFDPNIFAVATGIDEHNNYAVDFIEATRWIKRNLPYAKVSGGVSNVSFSFRGNDPVREAI EECCCEEEEEECCCCCCCEEEHHHHHHHHHHHCCCCCEECCCCCEEEEEECCCCHHHHHH HTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRDDSTDRLLEIADKFK HHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH TGAAKKEENLEWRSQPVEKRLAHALVHGITNFIVEDTEEARAKIAAAGGRPINVIEGPLM CCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEECCCCC DGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAQAGGDVRAKGKIVI CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEE ATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGLITPSL EEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEECCCCCCCCCH EEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVA HHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCCHHHHH SNLLSDEGATKYLDELKADYERIRAQHANKKALPLVTLAEARANKTKIDWARYTSVKPKF HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCHHHHCCCCHHH IGRRMFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKSMLARL HHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHH IQGRWLTANGVIALLPANTVGDDDIEIYTDDTRAEVLLTWHNLRQQSVRPVVDGVMRPNR HCCCEEECCCEEEEEECCCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC SLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLAEAFAE CHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH AMHARVRRELWGYAATEQLDNDALIAEQYAGIRPAPGYPACPDHLVKRAMFEALHTDEIG HHHHHHHHHHHCCHHHHHCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC MSVTDSLAMLPAASVSGFYLAHPDSTYFSVGKIGQDQLEDFARRTALSKADAERALAPLL CCHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]