Click here to switch to the map view.

The map label for this gene is metH2 [H]

Identifier: 53718026

GI number: 53718026

Start: 419469

End: 420548

Strand: Reverse

Name: metH2 [H]

Synonym: BPSL0386

Alternate gene names: 53718026

Gene position: 420548-419469 (Counterclockwise)

Preceding gene: 53718027

Following gene: 53718025

Centisome position: 10.32

GC content: 68.89

Gene sequence:

>1080_bases
ATGTCCGAGCCCACGCCGATCGCGCCTTTCGCCTCATCCGCCGCCCCCGCCGCGCCGTACACGCGCGGCGCGGCGCTGCC
GCAACTGCTCCGGCAGCGCATCCTGATCCTCGACGGCGCGATGGGCACGATGATCCAGCGCTACAAGCTCGACGAGGCCG
CGTATCGCGGCGAGCGCTTCAAGGATTTCCCGCGTGACGTGAAGGGCAACAACGAGCTGCTGTCGATCACGCAGCCGCGG
ATCATCCGCGAGATTCACGATCAGTACTTCGCCGCGGGCGCGGACATCGTCGAGACGAACACGTTCGGCGCGACGGCCGT
CGCGCAGGCCGATTACGGGATGGAAGCGCTCGTCGTCGAGATGAACGTTGCGTCGGCGGCGCTCGCGCGGGAATCGGCCG
CCAAATACGCGACGCCGGAAAAACCGCGCTTCGTCGCGGGCGCGATCGGGCCGACGCCGAAGACGGCGAGCATCTCGCCC
GACGTGAACGACCCCGGCGCGCGCAACGTCACGTTCGACGAGCTGCGCGATGCGTATTACCAGCAGGCGAAGGCGCTGCT
CGACGGCGGCGTCGACCTGTTCCTCGTCGAGACGATCTTCGACACGCTGAACGCGAAGGCCGCGCTCTTCGCGCTCGACC
AGCTGTTCGACGACACCGGCGAGCGACTGCCGATCATGATCTCGGGCACCGTCACCGACGCGTCCGGCCGCATCCTGTCC
GGCCAGACGGTCGAGGCGTTCTGGAATTCGCTGCGCCACGCGAAGCCGCTCACGTTCGGCCTGAACTGCGCGCTCGGCGC
GGCGCTGATGCGCCCGTACATCGCCGAGCTCGCGAAGCTGTGCGACACCTACGTGTCGTGCTACCCGAACGCGGGCCTGC
CGAACCCGATGAGCGACACCGGCTTCGACGAGACGCCCGACGTCACGTCCGGCCTCCTGAAGGAATTCGCGCAGGCGGGC
CTCGTCAACCTCGCGGGCGGCTGCTGCGGCACGACGCCCGAGCACATCGCGGCGATCGCGAAGGCGCTCGCCGAAGTGAA
GCCGCGCCGCTGGCCGAGCCAGTACAGCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
CCCGGCCCGCCGCGCGGGGGCTTTGTTTGTCCGGCGGCCGCCCACGCACGGCGGAAGCTGCGGCTACAATGACGGCTTTC
CGATTCGCCGGTAATCCGCC

Downstream 100 bases:

>100_bases
CGCGCGCGGCCCTCCCCACTCCAACCCGAATACGCACGCACCGCCATGACCGACCACACGATGCGCCTAGCCGGCCTCGA
GCCGTTCAACGTCACGTCCG

Product: putative 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSEPTPIAPFASSAAPAAPYTRGAALPQLLRQRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDVKGNNELLSITQPR
IIREIHDQYFAAGADIVETNTFGATAVAQADYGMEALVVEMNVASAALARESAAKYATPEKPRFVAGAIGPTPKTASISP
DVNDPGARNVTFDELRDAYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFDDTGERLPIMISGTVTDASGRILS
GQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAG
LVNLAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA

Sequences:

>Translated_359_residues
MSEPTPIAPFASSAAPAAPYTRGAALPQLLRQRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDVKGNNELLSITQPR
IIREIHDQYFAAGADIVETNTFGATAVAQADYGMEALVVEMNVASAALARESAAKYATPEKPRFVAGAIGPTPKTASISP
DVNDPGARNVTFDELRDAYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFDDTGERLPIMISGTVTDASGRILS
GQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAG
LVNLAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA
>Mature_358_residues
SEPTPIAPFASSAAPAAPYTRGAALPQLLRQRILILDGAMGTMIQRYKLDEAAYRGERFKDFPRDVKGNNELLSITQPRI
IREIHDQYFAAGADIVETNTFGATAVAQADYGMEALVVEMNVASAALARESAAKYATPEKPRFVAGAIGPTPKTASISPD
VNDPGARNVTFDELRDAYYQQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFDDTGERLPIMISGTVTDASGRILSG
QTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAGL
VNLAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=326, Percent_Identity=58.2822085889571, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1790450, Length=326, Percent_Identity=58.8957055214724, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1786456, Length=323, Percent_Identity=26.625386996904, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535405, Length=330, Percent_Identity=54.2424242424242, Blast_Score=359, Evalue=1e-99,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38459; Mature: 38328

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPTPIAPFASSAAPAAPYTRGAALPQLLRQRILILDGAMGTMIQRYKLDEAAYRGERF
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
KDFPRDVKGNNELLSITQPRIIREIHDQYFAAGADIVETNTFGATAVAQADYGMEALVVE
HHCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEEEE
MNVASAALARESAAKYATPEKPRFVAGAIGPTPKTASISPDVNDPGARNVTFDELRDAYY
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
QQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFDDTGERLPIMISGTVTDASGRILS
HHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEC
GQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDT
CCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GFDETPDVTSGLLKEFAQAGLVNLAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA
CCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
SEPTPIAPFASSAAPAAPYTRGAALPQLLRQRILILDGAMGTMIQRYKLDEAAYRGERF
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHH
KDFPRDVKGNNELLSITQPRIIREIHDQYFAAGADIVETNTFGATAVAQADYGMEALVVE
HHCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEEEE
MNVASAALARESAAKYATPEKPRFVAGAIGPTPKTASISPDVNDPGARNVTFDELRDAYY
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
QQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFDDTGERLPIMISGTVTDASGRILS
HHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEC
GQTVEAFWNSLRHAKPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDT
CCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GFDETPDVTSGLLKEFAQAGLVNLAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA
CCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]