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The map label for this gene is bioB [H]

Identifier: 53718004

GI number: 53718004

Start: 392668

End: 393687

Strand: Reverse

Name: bioB [H]

Synonym: BPSL0364

Alternate gene names: 53718004

Gene position: 393687-392668 (Counterclockwise)

Preceding gene: 53718005

Following gene: 53717997

Centisome position: 9.66

GC content: 67.06

Gene sequence:

>1020_bases
ATGACCGACATGACTGAAGCTCAAACCGCTTGCGCGACGACCGAAACACCGGTGGCCGCGCCCGCCGCGCCGCGCTGGCG
CGTCGCCGACGTGATCGCGCTCTACGAGCTGCCGTTCAACGATCTGCTGTTCCGCGCGCAGCAGACGCACCGCGAGCACT
TCGACGCGAACGCGATCCAGCTCTCGACGCTGCTGTCGATCAAGACGGGCGGCTGCGAGGAGGACTGCGGCTACTGCTCG
CAGTCCGCGCATCACGACACCGGCCTGAAGGCGGAGAAGCTGATGGAGGTGGACGCGGTGCTCGCCGCCGCGCGCACCGC
GAAGGAAAACGGCGCGACGCGCTTCTGCATGGGCGCCGCGTGGCGCAACCCGAAGGATCGCCACATCGAGCCGATCAAGG
AAATGATCCGCGGCGTGAAGGACATGGGCCTCGAGACGTGCGTGACGCTCGGCATGCTCGAGGAACACCAGGCGAAGGCG
CTGGCGGAGGCGGGCCTCGACTATTACAACCACAACCTCGACACGTCGCCCGAATTCTATGGGCAGATCATCTCGACGCG
CACGTACCAGGATCGCCTCGACACGCTCGAGCGCGTGCGCGACGCGGGCATCAACGTATGCTGCGGCGGGATCATCGGGA
TGGGCGAATCGCGCCGCGAGCGCGCGGGCCTGATCGCGCAGCTCGCGAACATGAACCCGTATCCGGAATCGGTGCCGATC
AACAATCTCGTCGCGATCGAAGGCACGCCGCTCGAGAACGCGCAGGCGCTCGATCCGTTCGAGTTCGTGCGTACGATCGC
GGTCGCGCGCATCACGATGCCGAAGGCGATGGTGCGCTTGTCGGCGGGCCGCGAGCAGCTCGACGACGCGATGCAGGCGC
TGTGCTTCCTCGCCGGCGCGAATTCGATGTTCTACGGCGACGTGCTGCTCACGACCGGCAATCCGCGCGCGGAAGCGGAC
CGCAAGCTGCTCGCGCGGCTCGGCATGTGGGCAAGCGAGGCGTCGCAGTTGAGCGCCTGA

Upstream 100 bases:

>100_bases
CTTGCTGAACGCGCTGCGCGCCGCCGCGCCGCGCTGACGTTTTAGCCGCTCGTTTCAACCGCTCTTTGCCGATTTTTCGC
ATCGATCCCTGTTCCGAAGG

Downstream 100 bases:

>100_bases
ACGCCGGCTGCCTTCCTGCAAAACAGCCGGGCGATGTCCCGGCTTTTTCGCGCGATGCGCGGCGCGGCACTCGCCGGCAA
CCGCTTCGGCCACGCCGCGG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTDMTEAQTACATTETPVAAPAAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQLSTLLSIKTGGCEEDCGYCS
QSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAAWRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKA
LAEAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPI
NNLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDAMQALCFLAGANSMFYGDVLLTTGNPRAEAD
RKLLARLGMWASEASQLSA

Sequences:

>Translated_339_residues
MTDMTEAQTACATTETPVAAPAAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQLSTLLSIKTGGCEEDCGYCS
QSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAAWRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKA
LAEAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPI
NNLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDAMQALCFLAGANSMFYGDVLLTTGNPRAEAD
RKLLARLGMWASEASQLSA
>Mature_338_residues
TDMTEAQTACATTETPVAAPAAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQLSTLLSIKTGGCEEDCGYCSQ
SAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAAWRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKAL
AEAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPIN
NLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDAMQALCFLAGANSMFYGDVLLTTGNPRAEADR
KLLARLGMWASEASQLSA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=316, Percent_Identity=61.3924050632911, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=50.1577287066246, Blast_Score=333, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37115; Mature: 36983

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMTEAQTACATTETPVAAPAAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQ
CCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEE
LSTLLSIKTGGCEEDCGYCSQSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAA
HHHHHEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
WRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKALAEAGLDYYNHNLDTSPEFY
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPI
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
NNLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDAMQALCFLAGA
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
NSMFYGDVLLTTGNPRAEADRKLLARLGMWASEASQLSA
CCEEECCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
TDMTEAQTACATTETPVAAPAAPRWRVADVIALYELPFNDLLFRAQQTHREHFDANAIQ
CCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEE
LSTLLSIKTGGCEEDCGYCSQSAHHDTGLKAEKLMEVDAVLAAARTAKENGATRFCMGAA
HHHHHEECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
WRNPKDRHIEPIKEMIRGVKDMGLETCVTLGMLEEHQAKALAEAGLDYYNHNLDTSPEFY
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
GQIISTRTYQDRLDTLERVRDAGINVCCGGIIGMGESRRERAGLIAQLANMNPYPESVPI
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
NNLVAIEGTPLENAQALDPFEFVRTIAVARITMPKAMVRLSAGREQLDDAMQALCFLAGA
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
NSMFYGDVLLTTGNPRAEADRKLLARLGMWASEASQLSA
CCEEECCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA