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The map label for this gene is nfrA [H]

Identifier: 52787699

GI number: 52787699

Start: 3847098

End: 3847847

Strand: Reverse

Name: nfrA [H]

Synonym: BLi04022

Alternate gene names: 52787699

Gene position: 3847847-3847098 (Counterclockwise)

Preceding gene: 52787701

Following gene: 52787698

Centisome position: 91.12

GC content: 47.07

Gene sequence:

>750_bases
ATGAATAAAACGATTGAAACTATTTTAAATCACCGTTCGATCAGGTCTTTTACAGATCGGCTCCTGACCGAAGAAGAGGT
TCGCCTGCTTGTGGAGAGCGCTCAAAGCGCATCGACGTCAAGCTATATTCAAGCATATTCAATCATCGGTGTCACCGATC
CGGACAAGAAGCGCAAGCTTGCGGAATTGGCCGGGAACCAGCCATATGTTGAAAAAAACGGCCATCTGTTTGTATTTTGC
GCAGATCTTTACAGGCATGACAAAATCGCTCGGAAAAAGGGTGTAGATATTACAGCTTCTCTTGAAGGGACGGAAACCTT
TATGGTCAGCGTCATCGATGCAGCGCTTGCCGCTCAAAATATGTCAATAGCGGCCGAATCAATGGGCCTCGGCATCTGCT
ATATCGGTGGGATTCGCAACAATTTGAACGAAGTCTCCGAGCTTCTTGAAACACCTGAATACGTTCTTCCGCTGTTCGGG
CTTGTCGTCGGCCAGCCGGCCAACCCTTCGGCCAAAAAACCGCGTCTGCCGCTTGATCACATTTATCATGAAAATGTCTA
TCAAGCGGATGATGCCGAGTTTGAAAAGCATTTGTCCGCATATGACGAAACGATTTCAAACTATTATCAAAAGCGGACGG
ACGGCAAACGGTCTGACAAATGGACAGACCAGATCACCGGCAGCCTCAAAAAGCCGCCGCGTGCGTATATGAATGAATTC
GTTAAAGGAAAAGGTTTGAACAAAAGGTAA

Upstream 100 bases:

>100_bases
ATTTTACCATATGAAATTGAATCCATCTGCAGTCCGCCAAACCGTTTTGCTTTAGAGAAAAGCAGATATTGGATATGATA
AAGGTTGAGGAGGTAAGAGG

Downstream 100 bases:

>100_bases
GGAACCTCCCCTCCGACTCGGAGGGGACTTATCTTATCAGCATGTATTGAAGGAAAAGATAAAACATCAGCCAAAAGGGC
AGACTGAAAAGGATTCCGAA

Product: NfrA

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNKTIETILNHRSIRSFTDRLLTEEEVRLLVESAQSASTSSYIQAYSIIGVTDPDKKRKLAELAGNQPYVEKNGHLFVFC
ADLYRHDKIARKKGVDITASLEGTETFMVSVIDAALAAQNMSIAAESMGLGICYIGGIRNNLNEVSELLETPEYVLPLFG
LVVGQPANPSAKKPRLPLDHIYHENVYQADDAEFEKHLSAYDETISNYYQKRTDGKRSDKWTDQITGSLKKPPRAYMNEF
VKGKGLNKR

Sequences:

>Translated_249_residues
MNKTIETILNHRSIRSFTDRLLTEEEVRLLVESAQSASTSSYIQAYSIIGVTDPDKKRKLAELAGNQPYVEKNGHLFVFC
ADLYRHDKIARKKGVDITASLEGTETFMVSVIDAALAAQNMSIAAESMGLGICYIGGIRNNLNEVSELLETPEYVLPLFG
LVVGQPANPSAKKPRLPLDHIYHENVYQADDAEFEKHLSAYDETISNYYQKRTDGKRSDKWTDQITGSLKKPPRAYMNEF
VKGKGLNKR
>Mature_249_residues
MNKTIETILNHRSIRSFTDRLLTEEEVRLLVESAQSASTSSYIQAYSIIGVTDPDKKRKLAELAGNQPYVEKNGHLFVFC
ADLYRHDKIARKKGVDITASLEGTETFMVSVIDAALAAQNMSIAAESMGLGICYIGGIRNNLNEVSELLETPEYVLPLFG
LVVGQPANPSAKKPRLPLDHIYHENVYQADDAEFEKHLSAYDETISNYYQKRTDGKRSDKWTDQITGSLKKPPRAYMNEF
VKGKGLNKR

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=249, Percent_Identity=41.3654618473896, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 27949; Mature: 27949

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTIETILNHRSIRSFTDRLLTEEEVRLLVESAQSASTSSYIQAYSIIGVTDPDKKRKL
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCHHHHHH
AELAGNQPYVEKNGHLFVFCADLYRHDKIARKKGVDITASLEGTETFMVSVIDAALAAQN
HHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHH
MSIAAESMGLGICYIGGIRNNLNEVSELLETPEYVLPLFGLVVGQPANPSAKKPRLPLDH
HHHHHHHCCCEEEEECCHHCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
IYHENVYQADDAEFEKHLSAYDETISNYYQKRTDGKRSDKWTDQITGSLKKPPRAYMNEF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHH
VKGKGLNKR
HHCCCCCCC
>Mature Secondary Structure
MNKTIETILNHRSIRSFTDRLLTEEEVRLLVESAQSASTSSYIQAYSIIGVTDPDKKRKL
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCHHHHHH
AELAGNQPYVEKNGHLFVFCADLYRHDKIARKKGVDITASLEGTETFMVSVIDAALAAQN
HHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHH
MSIAAESMGLGICYIGGIRNNLNEVSELLETPEYVLPLFGLVVGQPANPSAKKPRLPLDH
HHHHHHHCCCEEEEECCHHCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
IYHENVYQADDAEFEKHLSAYDETISNYYQKRTDGKRSDKWTDQITGSLKKPPRAYMNEF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHH
VKGKGLNKR
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]