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The map label for this gene is nrgA [H]

Identifier: 52787566

GI number: 52787566

Start: 3709708

End: 3710919

Strand: Direct

Name: nrgA [H]

Synonym: BLi03888

Alternate gene names: 52787566

Gene position: 3709708-3710919 (Clockwise)

Preceding gene: 52787565

Following gene: 52787567

Centisome position: 87.85

GC content: 51.82

Gene sequence:

>1212_bases
ATGCAGATGGGGGATACAGTATTTATGTTCTTTTGCGCTTTGCTCGTGTGGCTGATGACGCCGGGCATCGCTTTATTTTA
CGGAGGCCTTGTAAGGAGCAAAAATGTGCTCAGCACCGTTATGCACAGTTTATCTTCAATTGCCATCGTTTCTATCATAT
GGATTTTGTTCGGCTATTCACTCGCTTTCGCTCCCGGAAACCCATTGATCGGGGGTTTGGATTGGATCGGACTGCACGGT
GTCGGTTTTGAACCCAATGCCGATTATTCGGAGACGATCCCGCATACGCTTTATATGATGTTCCAATTGACATTTGCCGT
CCTGACGACTGCGATTATTTCCGGAAGCTTTGCGGAGCGCATGCGCTTTCCGGCCTTTATTCTTTTCTCGGTGCTCTGGG
CGGTTTTCGTTTATTCGCCTGTCGCCCACTGGGTATGGGGCGGAGGCTGGCTTGACGAGCTGGGAGCTATTGATTTTGCC
GGCGGAAACGTCGTTCACATTTCTTCCGGGGTAGCCGGTCTGGTCGTTGCGATTGTTCTCGGCAAGCGGAAAAATATGAG
CGACACCGCCCCTCACAACCTCCTTCTGACCCTGCTCGGGGCGACTTTGATCTGGTTCGGATGGTTCGGTTTCAATGTCG
GAAGCGCGCTGACGATCGATGAAGTCGCCATGACCGCTTTTATCAACACGAATACGGCTGCGGCCGCGGGGCTGATCGGC
TGGATTTTGGCAGAATGGCTGATTAATAAGAAGCCGACGATGCTCGGCGCCGTCTCGGGAGCGATTGCGGGACTCGTCGC
GATTACGCCTGGCGCAGGTTTTGTCACGCCATTCTCATCTGTATGGATCGGGCTTATCGGAGGAATCGTCTGTTTCTGGG
GGGTGTTTTCGCTCAAGAAAAAATTCGGGTACGATGATGCGCTCGATGCCTTCGGACTTCACGGGCTCGGCGGGACATGG
GGCGGAATCGCGACAGGTTTGTTCGCGACGACATCTGTCAACGCAGACGGTGCCAACGGGCTTTTTTACGGAGACATCAG
TCTCCTTTGGAAACAGCTGATTGCGATTGCTGCAACATATTTGTTTGTCGCAATCGTCACATACGCTATTATTAAGATAG
TCAGTATCTTTTTTAAACTCCGTGCCTCAGAGGATGAAGAATCATTGGGCCTCGATCTTACGCTGCACGGCGAAAAAGCG
TATCAAGATTAA

Upstream 100 bases:

>100_bases
AAGGAAATCTGACATGTTCATCATGATATCTCCCTTTTGTCACTATAATGAAAGAAACAGAGATCTGCTCTCAATCTAAA
TGACTACGGAGGAATGAAAC

Downstream 100 bases:

>100_bases
TTTGTCAGGAGTGATGGTATGAGCGGTCAAATGTATAAAGTGGAAATTGTAACCCGTCCTACAAACTTTGAAAAGCTGAA
AACGGAGCTGGCCAAAATCG

Product: NrgA

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MQMGDTVFMFFCALLVWLMTPGIALFYGGLVRSKNVLSTVMHSLSSIAIVSIIWILFGYSLAFAPGNPLIGGLDWIGLHG
VGFEPNADYSETIPHTLYMMFQLTFAVLTTAIISGSFAERMRFPAFILFSVLWAVFVYSPVAHWVWGGGWLDELGAIDFA
GGNVVHISSGVAGLVVAIVLGKRKNMSDTAPHNLLLTLLGATLIWFGWFGFNVGSALTIDEVAMTAFINTNTAAAAGLIG
WILAEWLINKKPTMLGAVSGAIAGLVAITPGAGFVTPFSSVWIGLIGGIVCFWGVFSLKKKFGYDDALDAFGLHGLGGTW
GGIATGLFATTSVNADGANGLFYGDISLLWKQLIAIAATYLFVAIVTYAIIKIVSIFFKLRASEDEESLGLDLTLHGEKA
YQD

Sequences:

>Translated_403_residues
MQMGDTVFMFFCALLVWLMTPGIALFYGGLVRSKNVLSTVMHSLSSIAIVSIIWILFGYSLAFAPGNPLIGGLDWIGLHG
VGFEPNADYSETIPHTLYMMFQLTFAVLTTAIISGSFAERMRFPAFILFSVLWAVFVYSPVAHWVWGGGWLDELGAIDFA
GGNVVHISSGVAGLVVAIVLGKRKNMSDTAPHNLLLTLLGATLIWFGWFGFNVGSALTIDEVAMTAFINTNTAAAAGLIG
WILAEWLINKKPTMLGAVSGAIAGLVAITPGAGFVTPFSSVWIGLIGGIVCFWGVFSLKKKFGYDDALDAFGLHGLGGTW
GGIATGLFATTSVNADGANGLFYGDISLLWKQLIAIAATYLFVAIVTYAIIKIVSIFFKLRASEDEESLGLDLTLHGEKA
YQD
>Mature_403_residues
MQMGDTVFMFFCALLVWLMTPGIALFYGGLVRSKNVLSTVMHSLSSIAIVSIIWILFGYSLAFAPGNPLIGGLDWIGLHG
VGFEPNADYSETIPHTLYMMFQLTFAVLTTAIISGSFAERMRFPAFILFSVLWAVFVYSPVAHWVWGGGWLDELGAIDFA
GGNVVHISSGVAGLVVAIVLGKRKNMSDTAPHNLLLTLLGATLIWFGWFGFNVGSALTIDEVAMTAFINTNTAAAAGLIG
WILAEWLINKKPTMLGAVSGAIAGLVAITPGAGFVTPFSSVWIGLIGGIVCFWGVFSLKKKFGYDDALDAFGLHGLGGTW
GGIATGLFATTSVNADGANGLFYGDISLLWKQLIAIAATYLFVAIVTYAIIKIVSIFFKLRASEDEESLGLDLTLHGEKA
YQD

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=402, Percent_Identity=44.7761194029851, Blast_Score=324, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI17550182, Length=444, Percent_Identity=29.7297297297297, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI133931100, Length=430, Percent_Identity=27.6744186046512, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=432, Percent_Identity=27.3148148148148, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564042, Length=420, Percent_Identity=26.9047619047619, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6324187, Length=408, Percent_Identity=39.4607843137255, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6325396, Length=410, Percent_Identity=35.8536585365854, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=416, Percent_Identity=36.2980769230769, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI161078314, Length=420, Percent_Identity=29.5238095238095, Blast_Score=141, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43098; Mature: 43098

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMGDTVFMFFCALLVWLMTPGIALFYGGLVRSKNVLSTVMHSLSSIAIVSIIWILFGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFAPGNPLIGGLDWIGLHGVGFEPNADYSETIPHTLYMMFQLTFAVLTTAIISGSFAER
HEECCCCCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MRFPAFILFSVLWAVFVYSPVAHWVWGGGWLDELGAIDFAGGNVVHISSGVAGLVVAIVL
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEECCCCEEEECCCHHHHHHHHHH
GKRKNMSDTAPHNLLLTLLGATLIWFGWFGFNVGSALTIDEVAMTAFINTNTAAAAGLIG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
WILAEWLINKKPTMLGAVSGAIAGLVAITPGAGFVTPFSSVWIGLIGGIVCFWGVFSLKK
HHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFGYDDALDAFGLHGLGGTWGGIATGLFATTSVNADGANGLFYGDISLLWKQLIAIAATY
HCCCCHHHHHHCCCCCCCCHHHHHHHHHHEEECCCCCCCCEEEECHHHHHHHHHHHHHHH
LFVAIVTYAIIKIVSIFFKLRASEDEESLGLDLTLHGEKAYQD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCC
>Mature Secondary Structure
MQMGDTVFMFFCALLVWLMTPGIALFYGGLVRSKNVLSTVMHSLSSIAIVSIIWILFGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFAPGNPLIGGLDWIGLHGVGFEPNADYSETIPHTLYMMFQLTFAVLTTAIISGSFAER
HEECCCCCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MRFPAFILFSVLWAVFVYSPVAHWVWGGGWLDELGAIDFAGGNVVHISSGVAGLVVAIVL
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEECCCCEEEECCCHHHHHHHHHH
GKRKNMSDTAPHNLLLTLLGATLIWFGWFGFNVGSALTIDEVAMTAFINTNTAAAAGLIG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
WILAEWLINKKPTMLGAVSGAIAGLVAITPGAGFVTPFSSVWIGLIGGIVCFWGVFSLKK
HHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFGYDDALDAFGLHGLGGTWGGIATGLFATTSVNADGANGLFYGDISLLWKQLIAIAATY
HCCCCHHHHHHCCCCCCCCHHHHHHHHHHEEECCCCCCCCEEEECHHHHHHHHHHHHHHH
LFVAIVTYAIIKIVSIFFKLRASEDEESLGLDLTLHGEKAYQD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]