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The map label for this gene is nrgB [H]

Identifier: 52787567

GI number: 52787567

Start: 3710938

End: 3711288

Strand: Direct

Name: nrgB [H]

Synonym: BLi03889

Alternate gene names: 52787567

Gene position: 3710938-3711288 (Clockwise)

Preceding gene: 52787566

Following gene: 52787571

Centisome position: 87.88

GC content: 47.29

Gene sequence:

>351_bases
ATGAGCGGTCAAATGTATAAAGTGGAAATTGTAACCCGTCCTACAAACTTTGAAAAGCTGAAAACGGAGCTGGCCAAAAT
CGGGGTAACGTCCATTACGTTTTACAATGTACACGGATGCGGCCTGCAAAAAGGGCATACTGAGCTTTACCGCGGGGTCA
AACGCGAAAGCAATGTGTATGAAAGGTTAAAAGTCGAAATCGTCGTCAGCAAAGTGCCTGTCGAAAAGGTGGCCGAGACG
GCGCAAAACGTCCTGAAAACCGGGGAACCCGGTGACGGCAAGATCTTCATATACGAAATTAAAAACACCATCAACATCCG
CACAGGCGAGGAAGGTCCTGACGCTTTATAA

Upstream 100 bases:

>100_bases
TTTTAAACTCCGTGCCTCAGAGGATGAAGAATCATTGGGCCTCGATCTTACGCTGCACGGCGAAAAAGCGTATCAAGATT
AATTTGTCAGGAGTGATGGT

Downstream 100 bases:

>100_bases
AAAAGATCGAAGCTCCCGGCTGGAGTTAAGCCGGGAGCTTTTCTAATGTTACAAAACAGCATCAATAACACCGTATGCCT
TCGCTTCGTCCGCATCCATA

Product: NrgB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MSGQMYKVEIVTRPTNFEKLKTELAKIGVTSITFYNVHGCGLQKGHTELYRGVKRESNVYERLKVEIVVSKVPVEKVAET
AQNVLKTGEPGDGKIFIYEIKNTINIRTGEEGPDAL

Sequences:

>Translated_116_residues
MSGQMYKVEIVTRPTNFEKLKTELAKIGVTSITFYNVHGCGLQKGHTELYRGVKRESNVYERLKVEIVVSKVPVEKVAET
AQNVLKTGEPGDGKIFIYEIKNTINIRTGEEGPDAL
>Mature_115_residues
SGQMYKVEIVTRPTNFEKLKTELAKIGVTSITFYNVHGCGLQKGHTELYRGVKRESNVYERLKVEIVVSKVPVEKVAETA
QNVLKTGEPGDGKIFIYEIKNTINIRTGEEGPDAL

Specific function: Required for full induction of the nrgAB operon under conditions of ammonium limitation [H]

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=39.2857142857143, Blast_Score=91, Evalue=1e-20,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=35.7142857142857, Blast_Score=84, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12971; Mature: 12840

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQMYKVEIVTRPTNFEKLKTELAKIGVTSITFYNVHGCGLQKGHTELYRGVKRESNVY
CCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCHH
ERLKVEIVVSKVPVEKVAETAQNVLKTGEPGDGKIFIYEIKNTINIRTGEEGPDAL
HHEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCCCC
>Mature Secondary Structure 
SGQMYKVEIVTRPTNFEKLKTELAKIGVTSITFYNVHGCGLQKGHTELYRGVKRESNVY
CCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCHH
ERLKVEIVVSKVPVEKVAETAQNVLKTGEPGDGKIFIYEIKNTINIRTGEEGPDAL
HHEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]