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The map label for this gene is yrpB [H]

Identifier: 52786997

GI number: 52786997

Start: 3164045

End: 3165100

Strand: Reverse

Name: yrpB [H]

Synonym: BLi03299

Alternate gene names: 52786997

Gene position: 3165100-3164045 (Counterclockwise)

Preceding gene: 52786998

Following gene: 52786995

Centisome position: 74.96

GC content: 52.84

Gene sequence:

>1056_bases
ATGAATCAGTTCATGGAACGCCTCTCTTTGTCAAAGCCTGTGGTCCAGGCGCCGATGGCGGGCGGGCCGACAACACCAAG
GCTCGCCGCCGCCGTATCAGACTGCGGCGGCTTAGGCAGCCTTGCATCCGGCTATTTGACGCCGGAAGTGCTTCAACAAC
AGATTCTGGAAACGAAAAAGCTGACATCAGCCGGATTTCAAGTCAACCTCTTCATTCCGGAAAAACGGGAAACCGTCACC
CGGGAAGAATATGAAAGGTGGCAAGAGAAGATACCTTTGGCGCGGTCGGCTTCACCCGTGACAGATGAGAAACAAGATTG
GGACGATTTTTACGAGAAAATAGAAATCATTCTCAAAGAAGGAATCAGCGCCGTTTCATTTACGTTTGGCCCTCCGCCGG
CTGATGCCGTCAAGGAACTGAAAAACCGAAATTGCTGTTTAATGGGCACCGCCGTTTCAGTTGAAGAAGCCGTGCTGCTT
GAAGAACTGGGGATGGATGTCATCATTGTTCAAGGAAGTGAAGCGGGCGGCCACAGGGGAGCCTTCTTGAAAACGAAGGG
AGAGCCTGCTGTCGGCTCGATGGCTTTAATTCCCCAAGCGGCAGATCATGTGTCGGTTCCAGTTATCGCAGCGGGCGGCA
TTTTTGACAAAAGAGGTGTTGCTGCAGCTTTTGCCCTCGGCGCACAGGGCGTTCAAATCGGTACCGCTTTTTTGACGTGC
GAAGAAAGCGGAACACACCCTGCCTACAAACAAAAATTGTTCGAAGCGGTTGAAACCGACACAAGCCTGACCCGTTTGTT
CTCGGGGAAGCCGGCAAGAGGCATCGTCAATCAGTGGATGGAAGACAGACGCCAGGAAGAAGCTGAAGCGCTGCCTTATC
CTCTGCAAAACACGCTGACGCAACCGATGAGAAAACAGGCGAAGCTTGACGGGAATACGGATAACATGTCATTGTGGGCC
GGACAGAGCGTGCGGGCCACCGTCGAGCAAACGACGGTGAAGGAGCTGATGGATCAGCTTGTGCCGGCCGATGTGCTATC
TGATCATTTGAATTAA

Upstream 100 bases:

>100_bases
CCTCGGGACTTGGCAGGGCATATATTTCTGCGAGTTCGACGGCCCGAGGCACCGGACGTGCTATTTTAAAATTTTAGCCG
ATTAAAAGGAGGAAATAAAA

Downstream 100 bases:

>100_bases
TGCCGGAATATCAACCCGCGTCACTTGGGAAAGCAGATAGGCGGACGTCTGTGCATGAGGCTTTCTCAGCCCGTTCAGTT
TTTCAATGATTGTTTCGCCG

Product: YrpB

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MNQFMERLSLSKPVVQAPMAGGPTTPRLAAAVSDCGGLGSLASGYLTPEVLQQQILETKKLTSAGFQVNLFIPEKRETVT
REEYERWQEKIPLARSASPVTDEKQDWDDFYEKIEIILKEGISAVSFTFGPPPADAVKELKNRNCCLMGTAVSVEEAVLL
EELGMDVIIVQGSEAGGHRGAFLKTKGEPAVGSMALIPQAADHVSVPVIAAGGIFDKRGVAAAFALGAQGVQIGTAFLTC
EESGTHPAYKQKLFEAVETDTSLTRLFSGKPARGIVNQWMEDRRQEEAEALPYPLQNTLTQPMRKQAKLDGNTDNMSLWA
GQSVRATVEQTTVKELMDQLVPADVLSDHLN

Sequences:

>Translated_351_residues
MNQFMERLSLSKPVVQAPMAGGPTTPRLAAAVSDCGGLGSLASGYLTPEVLQQQILETKKLTSAGFQVNLFIPEKRETVT
REEYERWQEKIPLARSASPVTDEKQDWDDFYEKIEIILKEGISAVSFTFGPPPADAVKELKNRNCCLMGTAVSVEEAVLL
EELGMDVIIVQGSEAGGHRGAFLKTKGEPAVGSMALIPQAADHVSVPVIAAGGIFDKRGVAAAFALGAQGVQIGTAFLTC
EESGTHPAYKQKLFEAVETDTSLTRLFSGKPARGIVNQWMEDRRQEEAEALPYPLQNTLTQPMRKQAKLDGNTDNMSLWA
GQSVRATVEQTTVKELMDQLVPADVLSDHLN
>Mature_351_residues
MNQFMERLSLSKPVVQAPMAGGPTTPRLAAAVSDCGGLGSLASGYLTPEVLQQQILETKKLTSAGFQVNLFIPEKRETVT
REEYERWQEKIPLARSASPVTDEKQDWDDFYEKIEIILKEGISAVSFTFGPPPADAVKELKNRNCCLMGTAVSVEEAVLL
EELGMDVIIVQGSEAGGHRGAFLKTKGEPAVGSMALIPQAADHVSVPVIAAGGIFDKRGVAAAFALGAQGVQIGTAFLTC
EESGTHPAYKQKLFEAVETDTSLTRLFSGKPARGIVNQWMEDRRQEEAEALPYPLQNTLTQPMRKQAKLDGNTDNMSLWA
GQSVRATVEQTTVKELMDQLVPADVLSDHLN

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322609, Length=319, Percent_Identity=29.4670846394984, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 38061; Mature: 38061

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFMERLSLSKPVVQAPMAGGPTTPRLAAAVSDCGGLGSLASGYLTPEVLQQQILETKK
CHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHH
LTSAGFQVNLFIPEKRETVTREEYERWQEKIPLARSASPVTDEKQDWDDFYEKIEIILKE
HHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GISAVSFTFGPPPADAVKELKNRNCCLMGTAVSVEEAVLLEELGMDVIIVQGSEAGGHRG
CCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
AFLKTKGEPAVGSMALIPQAADHVSVPVIAAGGIFDKRGVAAAFALGAQGVQIGTAFLTC
CEEEECCCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEEE
EESGTHPAYKQKLFEAVETDTSLTRLFSGKPARGIVNQWMEDRRQEEAEALPYPLQNTLT
CCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QPMRKQAKLDGNTDNMSLWAGQSVRATVEQTTVKELMDQLVPADVLSDHLN
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MNQFMERLSLSKPVVQAPMAGGPTTPRLAAAVSDCGGLGSLASGYLTPEVLQQQILETKK
CHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHH
LTSAGFQVNLFIPEKRETVTREEYERWQEKIPLARSASPVTDEKQDWDDFYEKIEIILKE
HHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GISAVSFTFGPPPADAVKELKNRNCCLMGTAVSVEEAVLLEELGMDVIIVQGSEAGGHRG
CCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
AFLKTKGEPAVGSMALIPQAADHVSVPVIAAGGIFDKRGVAAAFALGAQGVQIGTAFLTC
CEEEECCCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEEE
EESGTHPAYKQKLFEAVETDTSLTRLFSGKPARGIVNQWMEDRRQEEAEALPYPLQNTLT
CCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QPMRKQAKLDGNTDNMSLWAGQSVRATVEQTTVKELMDQLVPADVLSDHLN
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]