Click here to switch to the map view.

The map label for this gene is ytbJ

Identifier: 52786826

GI number: 52786826

Start: 2984403

End: 2985608

Strand: Reverse

Name: ytbJ

Synonym: BLi03100

Alternate gene names: 52786826

Gene position: 2985608-2984403 (Counterclockwise)

Preceding gene: 52786827

Following gene: 52786825

Centisome position: 70.7

GC content: 47.68

Gene sequence:

>1206_bases
ATGAATGAGGACTACATTTTAATCAGATTTGGCGAAATTTCAACAAAAGGAAAGAATAGAAAGCTTTTTGTCGATCGCCT
GAAAAGAAATATCAAAATGGTCTTGCGCGATTTCCGCAACGTCCGTTATGAATCAACACGCGACAGAATGACTCTCGTAT
TAAACGGAGAAGACGCTGAAGCTGTAATGGCTCGTCTGAAACACGTTTTCGGCATCCAATCGTTCAGTCTTGCCGTGAAG
TGCCGGACAGATCTGGAAAGCATTAAAGAGACGGCGCTCGCTTCTGTTCAAGAACAATACAAGCCCGGGGATACCTTTAA
AGTATCGACGAAAAGGGCTTATAAGCAGTTTGAATTAAATACAAATGAGATGAATGCCGAGATCGGAGGACACATTTTGC
GCAACACCGACGATCTTACCGTTGACGTCCACTCCCCGGACATCCATTTGCGTATCGAAATTCGGGAAGATGCCACGTAT
TTGACATTCCGGGATGAAAAAGGTGCGGGCGGACTGCCGGTCGGAAGCGGAGGAAAAGCGATGCTTATGATATCCGGCGG
AATCGACAGCCCTGTTGCCGGGTTCTATGCCATGAAGCGCGGACTTGAGATCGAAGCCGTTCACTTTTTCAGCCCGCCAT
ATACAAGCGAGCGGGCCAAGCAAAAAGTGATCGATCTTACTAAGCGGCTTACGGCTTTTGGCGGAGACATCAAGCTTCAT
ATCGTTCCGTTTACAAAAACTCAAGAGCTCATTCAAAAGCAGATTCCCGAGAACTATTCCATGACGGCGACCAGAAGGCT
GATGCTCCAGATCGCCGACAAACTGCGCGAACGCCATAACGCGCTTGCGATCTTTACAGGGGAAAGCCTCGGCCAGGTGG
CAAGCCAGACGCTTGAAAGCATGTATGCCATCAACGCGGTCACGAGCACGCCGGTTTTGCGCCCTTTAATCGGCATGGAT
AAGACGGAAATCATCGAAAAAGCGAAGGAAATCGATACGTACGATATCAGCATACGTCCGTACGAAGACTGCTGCACGAT
CTTTACGCCTTCTGCGCCGAAAACGCGTCCGAAAAAAGAGAAAATCGAACACTTTGAAAGCTACACAGATTTCGAACCGC
TTATCAACGAAGCTGTGGAAAACACGGAAACGATTGTTTTGAGCAGCAAAGCGGAAACGAAAGATCAATTTGCGGATTTT
TTCTAA

Upstream 100 bases:

>100_bases
AGCATGACGTACCGGGATAATCATGAGATTGTCGGCCCGTTTATGACGGCCTTAAAAGAAAGTTTACACAAACTAAAAGG
AATTATGAGGTAGAGGCACA

Downstream 100 bases:

>100_bases
AGGAATATTCAATCAAACATCTTTGTCTGTTTTTGCATACAATTACCAAACATTCTTTGTATGAAGTCATGTGTTTTTAC
ACAATCTATACTCACAAGGA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNEDYILIRFGEISTKGKNRKLFVDRLKRNIKMVLRDFRNVRYESTRDRMTLVLNGEDAEAVMARLKHVFGIQSFSLAVK
CRTDLESIKETALASVQEQYKPGDTFKVSTKRAYKQFELNTNEMNAEIGGHILRNTDDLTVDVHSPDIHLRIEIREDATY
LTFRDEKGAGGLPVGSGGKAMLMISGGIDSPVAGFYAMKRGLEIEAVHFFSPPYTSERAKQKVIDLTKRLTAFGGDIKLH
IVPFTKTQELIQKQIPENYSMTATRRLMLQIADKLRERHNALAIFTGESLGQVASQTLESMYAINAVTSTPVLRPLIGMD
KTEIIEKAKEIDTYDISIRPYEDCCTIFTPSAPKTRPKKEKIEHFESYTDFEPLINEAVENTETIVLSSKAETKDQFADF
F

Sequences:

>Translated_401_residues
MNEDYILIRFGEISTKGKNRKLFVDRLKRNIKMVLRDFRNVRYESTRDRMTLVLNGEDAEAVMARLKHVFGIQSFSLAVK
CRTDLESIKETALASVQEQYKPGDTFKVSTKRAYKQFELNTNEMNAEIGGHILRNTDDLTVDVHSPDIHLRIEIREDATY
LTFRDEKGAGGLPVGSGGKAMLMISGGIDSPVAGFYAMKRGLEIEAVHFFSPPYTSERAKQKVIDLTKRLTAFGGDIKLH
IVPFTKTQELIQKQIPENYSMTATRRLMLQIADKLRERHNALAIFTGESLGQVASQTLESMYAINAVTSTPVLRPLIGMD
KTEIIEKAKEIDTYDISIRPYEDCCTIFTPSAPKTRPKKEKIEHFESYTDFEPLINEAVENTETIVLSSKAETKDQFADF
F
>Mature_401_residues
MNEDYILIRFGEISTKGKNRKLFVDRLKRNIKMVLRDFRNVRYESTRDRMTLVLNGEDAEAVMARLKHVFGIQSFSLAVK
CRTDLESIKETALASVQEQYKPGDTFKVSTKRAYKQFELNTNEMNAEIGGHILRNTDDLTVDVHSPDIHLRIEIREDATY
LTFRDEKGAGGLPVGSGGKAMLMISGGIDSPVAGFYAMKRGLEIEAVHFFSPPYTSERAKQKVIDLTKRLTAFGGDIKLH
IVPFTKTQELIQKQIPENYSMTATRRLMLQIADKLRERHNALAIFTGESLGQVASQTLESMYAINAVTSTPVLRPLIGMD
KTEIIEKAKEIDTYDISIRPYEDCCTIFTPSAPKTRPKKEKIEHFESYTDFEPLINEAVENTETIVLSSKAETKDQFADF
F

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=392, Percent_Identity=27.5510204081633, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_BACLD (Q65G52)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080242.1
- RefSeq:   YP_092655.1
- HSSP:   Q81KU0
- ProteinModelPortal:   Q65G52
- SMR:   Q65G52
- STRING:   Q65G52
- EnsemblBacteria:   EBBACT00000056433
- EnsemblBacteria:   EBBACT00000060936
- GeneID:   3029008
- GeneID:   3098754
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03100
- KEGG:   bli:BL00431
- NMPDR:   fig|279010.5.peg.73
- eggNOG:   COG0301
- GeneTree:   EBGT00050000001800
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   BLIC279010-1:BLI03100-MONOMER
- BioCyc:   BLIC279010:BL00431-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 45487; Mature: 45487

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS51165 THUMP

Important sites: BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEDYILIRFGEISTKGKNRKLFVDRLKRNIKMVLRDFRNVRYESTRDRMTLVLNGEDAE
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHH
AVMARLKHVFGIQSFSLAVKCRTDLESIKETALASVQEQYKPGDTFKVSTKRAYKQFELN
HHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCC
TNEMNAEIGGHILRNTDDLTVDVHSPDIHLRIEIREDATYLTFRDEKGAGGLPVGSGGKA
CCCCCHHHCCEEEECCCCEEEEEECCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCE
MLMISGGIDSPVAGFYAMKRGLEIEAVHFFSPPYTSERAKQKVIDLTKRLTAFGGDIKLH
EEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTKTQELIQKQIPENYSMTATRRLMLQIADKLRERHNALAIFTGESLGQVASQTLES
EEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHH
MYAINAVTSTPVLRPLIGMDKTEIIEKAKEIDTYDISIRPYEDCCTIFTPSAPKTRPKKE
HHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCEEEEEECCHHHHHEEECCCCCCCCCHHH
KIEHFESYTDFEPLINEAVENTETIVLSSKAETKDQFADFF
HHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHCCC
>Mature Secondary Structure
MNEDYILIRFGEISTKGKNRKLFVDRLKRNIKMVLRDFRNVRYESTRDRMTLVLNGEDAE
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHH
AVMARLKHVFGIQSFSLAVKCRTDLESIKETALASVQEQYKPGDTFKVSTKRAYKQFELN
HHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCC
TNEMNAEIGGHILRNTDDLTVDVHSPDIHLRIEIREDATYLTFRDEKGAGGLPVGSGGKA
CCCCCHHHCCEEEECCCCEEEEEECCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCE
MLMISGGIDSPVAGFYAMKRGLEIEAVHFFSPPYTSERAKQKVIDLTKRLTAFGGDIKLH
EEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTKTQELIQKQIPENYSMTATRRLMLQIADKLRERHNALAIFTGESLGQVASQTLES
EEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHH
MYAINAVTSTPVLRPLIGMDKTEIIEKAKEIDTYDISIRPYEDCCTIFTPSAPKTRPKKE
HHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCEEEEEECCHHHHHEEECCCCCCCCCHHH
KIEHFESYTDFEPLINEAVENTETIVLSSKAETKDQFADFF
HHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA