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The map label for this gene is nifZ [H]

Identifier: 52786827

GI number: 52786827

Start: 2985616

End: 2986761

Strand: Reverse

Name: nifZ [H]

Synonym: BLi03101

Alternate gene names: 52786827

Gene position: 2986761-2985616 (Counterclockwise)

Preceding gene: 52786831

Following gene: 52786826

Centisome position: 70.73

GC content: 44.94

Gene sequence:

>1146_bases
ATGATTTATCTTGATAATAGTTCAACAACAAAACCATATCCTGAATTATTACAAATTTATGAACAAGTAAATCAGCGCTT
CTTCGGCAACCCGTCGTCTCTGCACCGCCACGGCGTTGAAGCGGATCAATTGCTTTCTGAAGCGCGGAAACAGATTAAAT
CGGCGCTTTCGCTAAAAAATCATGATATCGTGTTTACGTCCGGCGCTTCAGAAGCCAATAACATGGCGCTTAAAGGCATT
GCTTTGGCACATGCGCAAAAAGGAAAGCATATCATTGCTTCCTCCATTGAACACCCGTCTGTGACGGAATCCTTGGAGCA
GCTGAAGACGGCCTTCGGCTTTGAGGTTACATATCTTCCTGTCGACGGCCATGGCTTTGTTTCTCTTGAAGACTTGAAAC
AAGCGCTCCGCAAAGACACGATTCTTGTAAGCATCATGCACGTCAACAATGAAACAGGCGTCATCCAGCCTCTGGAAGAA
ATCGGGAAAATCGTAAAAGAAAACACAAGGGCATACTTTCACGTTGATCATGTTCAGGGCATCAACAAGGTTCCGCTGGA
CATCTCCGGGGCAGCCATTGACCTTTGTACAATCTCGGGTCACAAATTTCACGGCTTAAAGGGAACAGGAGCCCTCATTC
TAAACGAACGGATCTCGCTTTTTCCTTTGATTTCCGGAGGAGAACAGCAGAACAACAGGCGTGCTGGAACTGAAAATACC
GCCGGCGCCGTTACACTTGCAAAAGCGATCAATTTGTCAAAACAGGATTATAACCATTATATAGAAGAAATAAAGGCGGC
AAAATCAGCTTTTATCAAGGAGCTAAAAGAGCTGAAAGGTGTTATATTAAATACGCCGGAAGAAAACAGCGCGCCGCATA
TCATCAATTTTTCCGTTCCGGGATTGAAGGCTGAGGTCCTTTTGCATATGCTAGAGGAGCGGGATATTTTCGTATCGACG
ACATCAGCATGCTCTTCAAAACAGCATAAGCCGAGCAAGGTGCTTTTAGAAATGGGAAAAGGTGAAGGTGCGGCCTCAAG
CAGCATCAGAATCAGCATGACGTACCGGGATAATCATGAGATTGTCGGCCCGTTTATGACGGCCTTAAAAGAAAGTTTAC
ACAAACTAAAAGGAATTATGAGGTAG

Upstream 100 bases:

>100_bases
AATCAGAGAAGTCAAACATTTATTTTTAATTTTCAATTACAAGTTGATATGATAACATATACAGTCATGAAAAAGAACGT
TCATTTTAAAGGAGTAGATC

Downstream 100 bases:

>100_bases
AGGCACAATGAATGAGGACTACATTTTAATCAGATTTGGCGAAATTTCAACAAAAGGAAAGAATAGAAAGCTTTTTGTCG
ATCGCCTGAAAAGAAATATC

Product: NifZ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MIYLDNSSTTKPYPELLQIYEQVNQRFFGNPSSLHRHGVEADQLLSEARKQIKSALSLKNHDIVFTSGASEANNMALKGI
ALAHAQKGKHIIASSIEHPSVTESLEQLKTAFGFEVTYLPVDGHGFVSLEDLKQALRKDTILVSIMHVNNETGVIQPLEE
IGKIVKENTRAYFHVDHVQGINKVPLDISGAAIDLCTISGHKFHGLKGTGALILNERISLFPLISGGEQQNNRRAGTENT
AGAVTLAKAINLSKQDYNHYIEEIKAAKSAFIKELKELKGVILNTPEENSAPHIINFSVPGLKAEVLLHMLEERDIFVST
TSACSSKQHKPSKVLLEMGKGEGAASSSIRISMTYRDNHEIVGPFMTALKESLHKLKGIMR

Sequences:

>Translated_381_residues
MIYLDNSSTTKPYPELLQIYEQVNQRFFGNPSSLHRHGVEADQLLSEARKQIKSALSLKNHDIVFTSGASEANNMALKGI
ALAHAQKGKHIIASSIEHPSVTESLEQLKTAFGFEVTYLPVDGHGFVSLEDLKQALRKDTILVSIMHVNNETGVIQPLEE
IGKIVKENTRAYFHVDHVQGINKVPLDISGAAIDLCTISGHKFHGLKGTGALILNERISLFPLISGGEQQNNRRAGTENT
AGAVTLAKAINLSKQDYNHYIEEIKAAKSAFIKELKELKGVILNTPEENSAPHIINFSVPGLKAEVLLHMLEERDIFVST
TSACSSKQHKPSKVLLEMGKGEGAASSSIRISMTYRDNHEIVGPFMTALKESLHKLKGIMR
>Mature_381_residues
MIYLDNSSTTKPYPELLQIYEQVNQRFFGNPSSLHRHGVEADQLLSEARKQIKSALSLKNHDIVFTSGASEANNMALKGI
ALAHAQKGKHIIASSIEHPSVTESLEQLKTAFGFEVTYLPVDGHGFVSLEDLKQALRKDTILVSIMHVNNETGVIQPLEE
IGKIVKENTRAYFHVDHVQGINKVPLDISGAAIDLCTISGHKFHGLKGTGALILNERISLFPLISGGEQQNNRRAGTENT
AGAVTLAKAINLSKQDYNHYIEEIKAAKSAFIKELKELKGVILNTPEENSAPHIINFSVPGLKAEVLLHMLEERDIFVST
TSACSSKQHKPSKVLLEMGKGEGAASSSIRISMTYRDNHEIVGPFMTALKESLHKLKGIMR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=356, Percent_Identity=38.2022471910112, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=31.0679611650485, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=37.0757180156658, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1787970, Length=398, Percent_Identity=23.1155778894472, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=22.6700251889169, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=357, Percent_Identity=36.6946778711485, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17533177, Length=313, Percent_Identity=33.5463258785943, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=379, Percent_Identity=36.9393139841689, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI20129463, Length=356, Percent_Identity=37.9213483146067, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41858; Mature: 41858

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNSSTTKPYPELLQIYEQVNQRFFGNPSSLHRHGVEADQLLSEARKQIKSALSLKN
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
HDIVFTSGASEANNMALKGIALAHAQKGKHIIASSIEHPSVTESLEQLKTAFGFEVTYLP
CCEEEECCCCCCCCEEHHHHHHHHHCCCCEEEHHCCCCCHHHHHHHHHHHHHCCEEEEEE
VDGHGFVSLEDLKQALRKDTILVSIMHVNNETGVIQPLEEIGKIVKENTRAYFHVDHVQG
ECCCCCEEHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEHHHCC
INKVPLDISGAAIDLCTISGHKFHGLKGTGALILNERISLFPLISGGEQQNNRRAGTENT
CCCCCEEECCCEEEEEEECCCEECCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCC
AGAVTLAKAINLSKQDYNHYIEEIKAAKSAFIKELKELKGVILNTPEENSAPHIINFSVP
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCC
GLKAEVLLHMLEERDIFVSTTSACSSKQHKPSKVLLEMGKGEGAASSSIRISMTYRDNHE
CHHHHHHHHHHHCCCEEEEECHHHCCCCCCCHHHHHEECCCCCCCCCCEEEEEEECCCCC
IVGPFMTALKESLHKLKGIMR
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIYLDNSSTTKPYPELLQIYEQVNQRFFGNPSSLHRHGVEADQLLSEARKQIKSALSLKN
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
HDIVFTSGASEANNMALKGIALAHAQKGKHIIASSIEHPSVTESLEQLKTAFGFEVTYLP
CCEEEECCCCCCCCEEHHHHHHHHHCCCCEEEHHCCCCCHHHHHHHHHHHHHCCEEEEEE
VDGHGFVSLEDLKQALRKDTILVSIMHVNNETGVIQPLEEIGKIVKENTRAYFHVDHVQG
ECCCCCEEHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEHHHCC
INKVPLDISGAAIDLCTISGHKFHGLKGTGALILNERISLFPLISGGEQQNNRRAGTENT
CCCCCEEECCCEEEEEEECCCEECCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCC
AGAVTLAKAINLSKQDYNHYIEEIKAAKSAFIKELKELKGVILNTPEENSAPHIINFSVP
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCC
GLKAEVLLHMLEERDIFVSTTSACSSKQHKPSKVLLEMGKGEGAASSSIRISMTYRDNHE
CHHHHHHHHHHHCCCEEEEECHHHCCCCCCCHHHHHEECCCCCCCCCCEEEEEEECCCCC
IVGPFMTALKESLHKLKGIMR
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]