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The map label for this gene is argH

Identifier: 52786810

GI number: 52786810

Start: 2969091

End: 2970470

Strand: Reverse

Name: argH

Synonym: BLi03083

Alternate gene names: 52786810

Gene position: 2970470-2969091 (Counterclockwise)

Preceding gene: 52786811

Following gene: 52786809

Centisome position: 70.35

GC content: 47.9

Gene sequence:

>1380_bases
ATGAAAAAGCTGTGGGGAGGCCGGTTCCAAAAAACACCGGAAAAATGGGTCGATGAGTTCGGCGCATCGATCCACTTTGA
CAAAACGCTTGTAAAAGAAGATATCGCAGGGTCTCTCGCTCATGCTTCCATGCTTGAAAAATGCGGCATTTTAACCGAAG
CAGAAGCGGTCAAAATAAAGGAAGGCCTGACATCCCTTTTGCACAAAGCGGAAAAAAACGAACTCGATTTTTCAGTCGAT
TGCGAAGATATTCACCTCAACCTAGAAAAAATGCTCATTGATGAAATCGGGCCGCTCGGCGGAAAGCTTCATACGGCCAG
AAGCCGAAACGACCAGGTGGCGACGGATATGCACCTGTATTTAAAAGGGCACACTGAGCATATCATCGAGCTGATTACGG
CTTTTCAACACGTTCTGATCGAAAAAGCGGAAGAACACGTGGAGACGATACTGCCGGGATACACCCATTTGCAGCGGGCC
CAGCCGATCTCGTTCGCGCATCATTTGCTGGCTTATTTCTGGATGCTGGAGCGCGATAAAGAACGCTATCATGATTCATT
CAAGCGGATTAACAAGTCTCCTCTCGGATGCGGGGCGCTCGCCGGAACGACGTTTCCGATCGACCGTGAATATTCCGCAG
ACCTGCTCGGCTTTGATTCGATATATGAAAACAGCCTGGACGGCGTCAGCGACAGGGATTTCATCCTTGAATTTTTAAGC
GCGAGCAGCATTCTGATGATGCATCTGTCAAGATTCTCCGAAGAAATCATTCTTTGGTGCTCGCAGGAATTTAAATTCAT
TGAGCTTGATGACACCTACGCGACGGGAAGCAGCATGATGCCGCAAAAGAAAAACCCGGACATGGCGGAGCTCATCCGCG
GGAAAACAGGACGCGTATACGGTGATCTGATCGGACTCCTCACGATTATGAAGGGGCTCCCGTTAGCATACAATAAAGAT
TTGCAGGAAGACAAAGAAGGTATGTTTGATACTGTGAAAACGGTCGAAGGAAGCCTTGAGATTTTTGCCGGCATGATTAA
AACGATGACAGTGAACAAAAATATGATGAAAAAAGCAACGGAGCAGGATTTTTCAAATGCAACTGAACTTGCCGACTATC
TGGCGAAAAAGGGCATGCCCTTCAGAGAAGCGCACGAAGTCGTCGGAAGACTTGTCTTCACCTGCATCGAAAAGGGCATT
TATCTCAGCGGCCTGCCGTTTGAAGAGTTCAAAAAGGCAAGCGGGCTGTTTGAAGAAGACGTCTACATCGTGCTCGACCC
GCACCATGCCGTCGAAAAAAGAATGAGCGAAGGCGGGACGGGATTCAAAAAGGTGACAGAAGCCATTCAAAAGGCGAAGC
AGTGTTTGAACAATTTTTAA

Upstream 100 bases:

>100_bases
ACGATGCATTCGATCATCACGCTGCAGTCGGCTTTATCGAGCTGTGGGGACTGCCGACGAAAGTAAACAGCATCGTCCAA
AAAAAGGAGCAGATTAAAGC

Downstream 100 bases:

>100_bases
CACTTCCCGATTCGATTCATAACTGTGCGGCTTCATACGCAAAATAAATTTGTGAATCGAAAAGGGGTGTGGCAATGAAA
CAAGATCAGTCAAAACACGG

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKKLWGGRFQKTPEKWVDEFGASIHFDKTLVKEDIAGSLAHASMLEKCGILTEAEAVKIKEGLTSLLHKAEKNELDFSVD
CEDIHLNLEKMLIDEIGPLGGKLHTARSRNDQVATDMHLYLKGHTEHIIELITAFQHVLIEKAEEHVETILPGYTHLQRA
QPISFAHHLLAYFWMLERDKERYHDSFKRINKSPLGCGALAGTTFPIDREYSADLLGFDSIYENSLDGVSDRDFILEFLS
ASSILMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVYGDLIGLLTIMKGLPLAYNKD
LQEDKEGMFDTVKTVEGSLEIFAGMIKTMTVNKNMMKKATEQDFSNATELADYLAKKGMPFREAHEVVGRLVFTCIEKGI
YLSGLPFEEFKKASGLFEEDVYIVLDPHHAVEKRMSEGGTGFKKVTEAIQKAKQCLNNF

Sequences:

>Translated_459_residues
MKKLWGGRFQKTPEKWVDEFGASIHFDKTLVKEDIAGSLAHASMLEKCGILTEAEAVKIKEGLTSLLHKAEKNELDFSVD
CEDIHLNLEKMLIDEIGPLGGKLHTARSRNDQVATDMHLYLKGHTEHIIELITAFQHVLIEKAEEHVETILPGYTHLQRA
QPISFAHHLLAYFWMLERDKERYHDSFKRINKSPLGCGALAGTTFPIDREYSADLLGFDSIYENSLDGVSDRDFILEFLS
ASSILMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVYGDLIGLLTIMKGLPLAYNKD
LQEDKEGMFDTVKTVEGSLEIFAGMIKTMTVNKNMMKKATEQDFSNATELADYLAKKGMPFREAHEVVGRLVFTCIEKGI
YLSGLPFEEFKKASGLFEEDVYIVLDPHHAVEKRMSEGGTGFKKVTEAIQKAKQCLNNF
>Mature_459_residues
MKKLWGGRFQKTPEKWVDEFGASIHFDKTLVKEDIAGSLAHASMLEKCGILTEAEAVKIKEGLTSLLHKAEKNELDFSVD
CEDIHLNLEKMLIDEIGPLGGKLHTARSRNDQVATDMHLYLKGHTEHIIELITAFQHVLIEKAEEHVETILPGYTHLQRA
QPISFAHHLLAYFWMLERDKERYHDSFKRINKSPLGCGALAGTTFPIDREYSADLLGFDSIYENSLDGVSDRDFILEFLS
ASSILMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVYGDLIGLLTIMKGLPLAYNKD
LQEDKEGMFDTVKTVEGSLEIFAGMIKTMTVNKNMMKKATEQDFSNATELADYLAKKGMPFREAHEVVGRLVFTCIEKGI
YLSGLPFEEFKKASGLFEEDVYIVLDPHHAVEKRMSEGGTGFKKVTEAIQKAKQCLNNF

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=455, Percent_Identity=44.3956043956044, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI68303542, Length=455, Percent_Identity=44.3956043956044, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI68303549, Length=455, Percent_Identity=41.7582417582418, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI68303547, Length=455, Percent_Identity=41.5384615384615, Blast_Score=359, Evalue=3e-99,
Organism=Escherichia coli, GI1790398, Length=455, Percent_Identity=49.010989010989, Blast_Score=440, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321806, Length=459, Percent_Identity=43.5729847494553, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI221473854, Length=457, Percent_Identity=38.074398249453, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI78706858, Length=457, Percent_Identity=38.074398249453, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_BACLD (Q65G68)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080226.1
- RefSeq:   YP_092639.1
- ProteinModelPortal:   Q65G68
- SMR:   Q65G68
- STRING:   Q65G68
- EnsemblBacteria:   EBBACT00000054359
- EnsemblBacteria:   EBBACT00000058854
- GeneID:   3028973
- GeneID:   3098707
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03083
- KEGG:   bli:BL00416
- NMPDR:   fig|279010.5.peg.3444
- eggNOG:   COG0165
- GeneTree:   EBGT00070000031828
- HOGENOM:   HBG539632
- OMA:   MAEDLIF
- ProtClustDB:   PRK00855
- BioCyc:   BLIC279010-1:BLI03083-MONOMER
- BioCyc:   BLIC279010:BL00416-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 51981; Mature: 51981

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLWGGRFQKTPEKWVDEFGASIHFDKTLVKEDIAGSLAHASMLEKCGILTEAEAVKIK
CCCCCCCCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EGLTSLLHKAEKNELDFSVDCEDIHLNLEKMLIDEIGPLGGKLHTARSRNDQVATDMHLY
HHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHCEEEE
LKGHTEHIIELITAFQHVLIEKAEEHVETILPGYTHLQRAQPISFAHHLLAYFWMLERDK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
ERYHDSFKRINKSPLGCGALAGTTFPIDREYSADLLGFDSIYENSLDGVSDRDFILEFLS
HHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
ASSILMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVY
HHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCHHH
GDLIGLLTIMKGLPLAYNKDLQEDKEGMFDTVKTVEGSLEIFAGMIKTMTVNKNMMKKAT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EQDFSNATELADYLAKKGMPFREAHEVVGRLVFTCIEKGIYLSGLPFEEFKKASGLFEED
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHCCCCCCC
VYIVLDPHHAVEKRMSEGGTGFKKVTEAIQKAKQCLNNF
EEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLWGGRFQKTPEKWVDEFGASIHFDKTLVKEDIAGSLAHASMLEKCGILTEAEAVKIK
CCCCCCCCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EGLTSLLHKAEKNELDFSVDCEDIHLNLEKMLIDEIGPLGGKLHTARSRNDQVATDMHLY
HHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHCEEEE
LKGHTEHIIELITAFQHVLIEKAEEHVETILPGYTHLQRAQPISFAHHLLAYFWMLERDK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
ERYHDSFKRINKSPLGCGALAGTTFPIDREYSADLLGFDSIYENSLDGVSDRDFILEFLS
HHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
ASSILMMHLSRFSEEIILWCSQEFKFIELDDTYATGSSMMPQKKNPDMAELIRGKTGRVY
HHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCHHH
GDLIGLLTIMKGLPLAYNKDLQEDKEGMFDTVKTVEGSLEIFAGMIKTMTVNKNMMKKAT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EQDFSNATELADYLAKKGMPFREAHEVVGRLVFTCIEKGIYLSGLPFEEFKKASGLFEED
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHCCCCCCC
VYIVLDPHHAVEKRMSEGGTGFKKVTEAIQKAKQCLNNF
EEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA