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The map label for this gene is argG

Identifier: 52786811

GI number: 52786811

Start: 2970467

End: 2971678

Strand: Reverse

Name: argG

Synonym: BLi03084

Alternate gene names: 52786811

Gene position: 2971678-2970467 (Counterclockwise)

Preceding gene: 52786812

Following gene: 52786810

Centisome position: 70.37

GC content: 49.75

Gene sequence:

>1212_bases
ATGACGCAAAAGAAAAAAGTGGTATTAGCATATTCAGGAGGACTTGATACTTCTGTCGCGATAAAGTGGCTTCAGGAACA
GGGATATGATGTAGTGGCCTGCTGCCTTGACGTCGGAGAGGGAAAGGATCTCGCCTTTGTTCAGCAAAAAGCGCTTCAAG
TCGGAGCCTCTAATTCTTATGTTATCGATGCAAAAGAAGAATTTGCGAAAGAGTTTGCGCTGACCGCCCTCCAGGCCCAC
ACGCTTTATGAAGGAAAGTATCCGCTCGTATCAGCCCTGTCCAGACCGCTGATCGCCAAAAAGCTCGTCGAAGTGGCTGA
GAAAGAAAACGCGCAAGCCGTGGCTCACGGCTGCACAGGCAAAGGGAATGACCAAGTGCGCTTCGAGGTGTCGATTAAAT
CGCTCAATCCGGATTTGGAGGTGCTGGCACCTGTGCGCGAATGGAAATGGTCTCGCGACGAAGAGATCGCGTATGCAGAA
AAGCATGGCATTCCAATTCCGATCAATCTTGACAGCCCGTATTCAATCGACCAGAACCTGTGGGGCAGAAGCAATGAATG
CGGAGTCCTGGAAGATCCTTGGGCCGCGCCTCCTGAAGGAGCGTATGATTTGACGAAGCCTCTTGAAAAAACACCGGATA
CTCCTGATGTGATCGAAATCGCTTTTGAAGAAGGCGTTCCCGTATCCATTGACGGGACTCACTACCCGCTGTCAGACTTG
ATTTTAAAGCTGAATGAACTGGCGGGCGCACATGGAGTGGGGCGCATCGACCATGTTGAAAACCGCCTTGTCGGCATCAA
ATCGCGCGAAGTTTACGAATGCCCGGGCGCGATGACTTTGATTAAGGCGCACAAGGAGCTTGAAGATTTGACATTGGTAA
AAGAAGTGGCGCATTTCAAACCTATAATCGAACAAAAAATGGCTGAAGTGATCTATAACGGCCTCTGGTTTTCTCCGCTT
AAAGACGCGCTTTCCGGTTTCCTGAAAGAAACGCAGAAGCATGTCACGGGCGTCGTCCGCGTCAAGCTGTTTAAAGGCCA
TGCGATCGTTGAAGGGCGTAAGTCCGAATACTCCCTGTATGATGAAAAGCTTGCGACGTATACAAAAGACGATGCATTCG
ATCATCACGCTGCAGTCGGCTTTATCGAGCTGTGGGGACTGCCGACGAAAGTAAACAGCATCGTCCAAAAAAAGGAGCAG
ATTAAAGCATGA

Upstream 100 bases:

>100_bases
CCAGACTTCCTTGTTAAAGTGTATACATATTAAATGTATTTTTATTAAATAAAACTTATTTTTATACAAAAGAGCACCGA
GATGAGAGGAGTTTTTCGAA

Downstream 100 bases:

>100_bases
AAAAGCTGTGGGGAGGCCGGTTCCAAAAAACACCGGAAAAATGGGTCGATGAGTTCGGCGCATCGATCCACTTTGACAAA
ACGCTTGTAAAAGAAGATAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTQKKKVVLAYSGGLDTSVAIKWLQEQGYDVVACCLDVGEGKDLAFVQQKALQVGASNSYVIDAKEEFAKEFALTALQAH
TLYEGKYPLVSALSRPLIAKKLVEVAEKENAQAVAHGCTGKGNDQVRFEVSIKSLNPDLEVLAPVREWKWSRDEEIAYAE
KHGIPIPINLDSPYSIDQNLWGRSNECGVLEDPWAAPPEGAYDLTKPLEKTPDTPDVIEIAFEEGVPVSIDGTHYPLSDL
ILKLNELAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFKPIIEQKMAEVIYNGLWFSPL
KDALSGFLKETQKHVTGVVRVKLFKGHAIVEGRKSEYSLYDEKLATYTKDDAFDHHAAVGFIELWGLPTKVNSIVQKKEQ
IKA

Sequences:

>Translated_403_residues
MTQKKKVVLAYSGGLDTSVAIKWLQEQGYDVVACCLDVGEGKDLAFVQQKALQVGASNSYVIDAKEEFAKEFALTALQAH
TLYEGKYPLVSALSRPLIAKKLVEVAEKENAQAVAHGCTGKGNDQVRFEVSIKSLNPDLEVLAPVREWKWSRDEEIAYAE
KHGIPIPINLDSPYSIDQNLWGRSNECGVLEDPWAAPPEGAYDLTKPLEKTPDTPDVIEIAFEEGVPVSIDGTHYPLSDL
ILKLNELAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFKPIIEQKMAEVIYNGLWFSPL
KDALSGFLKETQKHVTGVVRVKLFKGHAIVEGRKSEYSLYDEKLATYTKDDAFDHHAAVGFIELWGLPTKVNSIVQKKEQ
IKA
>Mature_402_residues
TQKKKVVLAYSGGLDTSVAIKWLQEQGYDVVACCLDVGEGKDLAFVQQKALQVGASNSYVIDAKEEFAKEFALTALQAHT
LYEGKYPLVSALSRPLIAKKLVEVAEKENAQAVAHGCTGKGNDQVRFEVSIKSLNPDLEVLAPVREWKWSRDEEIAYAEK
HGIPIPINLDSPYSIDQNLWGRSNECGVLEDPWAAPPEGAYDLTKPLEKTPDTPDVIEIAFEEGVPVSIDGTHYPLSDLI
LKLNELAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFKPIIEQKMAEVIYNGLWFSPLK
DALSGFLKETQKHVTGVVRVKLFKGHAIVEGRKSEYSLYDEKLATYTKDDAFDHHAAVGFIELWGLPTKVNSIVQKKEQI
KA

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=408, Percent_Identity=43.6274509803922, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI16950633, Length=408, Percent_Identity=43.6274509803922, Blast_Score=327, Evalue=1e-89,
Organism=Escherichia coli, GI1789563, Length=414, Percent_Identity=24.8792270531401, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324514, Length=403, Percent_Identity=39.2059553349876, Blast_Score=277, Evalue=3e-75,
Organism=Drosophila melanogaster, GI21358151, Length=396, Percent_Identity=40.1515151515151, Blast_Score=287, Evalue=1e-77,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_BACLD (Q65G67)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080227.1
- RefSeq:   YP_092640.1
- ProteinModelPortal:   Q65G67
- SMR:   Q65G67
- STRING:   Q65G67
- EnsemblBacteria:   EBBACT00000057257
- EnsemblBacteria:   EBBACT00000061828
- GeneID:   3028521
- GeneID:   3098708
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi03084
- KEGG:   bli:BL00417
- NMPDR:   fig|279010.5.peg.3445
- eggNOG:   COG0137
- GeneTree:   EBGT00050000002857
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   BLIC279010-1:BLI03084-MONOMER
- BioCyc:   BLIC279010:BL00417-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44687; Mature: 44556

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 87-87 BINDING 117-117 BINDING 119-119 BINDING 123-123 BINDING 123-123 BINDING 124-124 BINDING 127-127 BINDING 175-175 BINDING 184-184 BINDING 260-260 BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKKKVVLAYSGGLDTSVAIKWLQEQGYDVVACCLDVGEGKDLAFVQQKALQVGASNSY
CCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCE
VIDAKEEFAKEFALTALQAHTLYEGKYPLVSALSRPLIAKKLVEVAEKENAQAVAHGCTG
EEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCC
KGNDQVRFEVSIKSLNPDLEVLAPVREWKWSRDEEIAYAEKHGIPIPINLDSPYSIDQNL
CCCCEEEEEEEEECCCCCHHHHCCHHHCCCCCCCCCCHHHHCCCEEEEECCCCCCCCHHH
WGRSNECGVLEDPWAAPPEGAYDLTKPLEKTPDTPDVIEIAFEEGVPVSIDGTHYPLSDL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCHHHH
ILKLNELAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFK
HHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PIIEQKMAEVIYNGLWFSPLKDALSGFLKETQKHVTGVVRVKLFKGHAIVEGRKSEYSLY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEECCCCCCHHHH
DEKLATYTKDDAFDHHAAVGFIELWGLPTKVNSIVQKKEQIKA
HHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQKKKVVLAYSGGLDTSVAIKWLQEQGYDVVACCLDVGEGKDLAFVQQKALQVGASNSY
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCE
VIDAKEEFAKEFALTALQAHTLYEGKYPLVSALSRPLIAKKLVEVAEKENAQAVAHGCTG
EEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCC
KGNDQVRFEVSIKSLNPDLEVLAPVREWKWSRDEEIAYAEKHGIPIPINLDSPYSIDQNL
CCCCEEEEEEEEECCCCCHHHHCCHHHCCCCCCCCCCHHHHCCCEEEEECCCCCCCCHHH
WGRSNECGVLEDPWAAPPEGAYDLTKPLEKTPDTPDVIEIAFEEGVPVSIDGTHYPLSDL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCHHHH
ILKLNELAGAHGVGRIDHVENRLVGIKSREVYECPGAMTLIKAHKELEDLTLVKEVAHFK
HHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PIIEQKMAEVIYNGLWFSPLKDALSGFLKETQKHVTGVVRVKLFKGHAIVEGRKSEYSLY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEECCCCCCHHHH
DEKLATYTKDDAFDHHAAVGFIELWGLPTKVNSIVQKKEQIKA
HHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA