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The map label for this gene is pyrF

Identifier: 52785534

GI number: 52785534

Start: 1734066

End: 1734785

Strand: Direct

Name: pyrF

Synonym: BLi01775

Alternate gene names: 52785534

Gene position: 1734066-1734785 (Clockwise)

Preceding gene: 52785533

Following gene: 52785535

Centisome position: 41.07

GC content: 53.61

Gene sequence:

>720_bases
ATGAACAATACGCCGCCCATCATCGCGCTTGATTTTGCATCAGCACAGGAAACATATGCGTTTTTGGACCGGTTTCAAGG
AGAAGAGCTGTTCGTTAAGGTGGGCATGGAGCTTTTTTATCAGGAAGGACCTGCCATCCTTGAAAATCTGCAGGAGCGCG
GCTGCCGCATTTTCCTCGATCTAAAGTGCCACGACATTCCAACGACCGTATACAAGGCGATGAAAAGGCTGGCGGGATTT
GGCGTCAGTCTTGTCAATGTGCACGCAGCAGGCGGAAAGCAAATGATGGAGTCGGCGCTTGAAGGCCTTGAAGCGGGGAC
TCCGGCAGGCCAAAAACGCCCTTCATTAATTGCCGTGACTCAGCTGACAAGCACGTCCTCAGAAATGCTTCAGCGTGAGC
TCCTGATTGAAACGCCGCTTCTCGATACAGTTGTCCACTACAGCCGGCTGGCTGAAGAAAGCGGTCTTGACGGCGTCGTC
TGCTCCGTTCATGAAGCCGAACACATTTACCGGGCTGTGTCTGTAGATTTTCTGACCGTGACACCCGGAATTCGCATGGC
AGATGACAAAAACAACGACCAGGTCCGGGTGGCGACGCCTGGCTACGCAAGGGAAAAAGGCGTGTCGGCGATCGTTGTCG
GCCGTTCGATTACCCAGGCGGAAGATCCTGTGTCAGCCTACAGAAGAATAGGACATGAGTGGGAGGGAACCAAGGCATGA

Upstream 100 bases:

>100_bases
AATCCGTTTGCTTGTCCCGACATCATCGACGATTTGCCGCGCGTTTTAGAAAAATACGGATACCGTTCGTTGAACGAGTG
TATCGGAAGGAGCTGGAAGG

Downstream 100 bases:

>100_bases
AAACAGACATTGCAAACCATTTATTAAAAATCAAAGCGGTTTTCCTGCGCCCGAACGAACCGTTTACATGGGCGAGCGGC
ATTCTGTCGCCCATCTACTG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNNTPPIIALDFASAQETYAFLDRFQGEELFVKVGMELFYQEGPAILENLQERGCRIFLDLKCHDIPTTVYKAMKRLAGF
GVSLVNVHAAGGKQMMESALEGLEAGTPAGQKRPSLIAVTQLTSTSSEMLQRELLIETPLLDTVVHYSRLAEESGLDGVV
CSVHEAEHIYRAVSVDFLTVTPGIRMADDKNNDQVRVATPGYAREKGVSAIVVGRSITQAEDPVSAYRRIGHEWEGTKA

Sequences:

>Translated_239_residues
MNNTPPIIALDFASAQETYAFLDRFQGEELFVKVGMELFYQEGPAILENLQERGCRIFLDLKCHDIPTTVYKAMKRLAGF
GVSLVNVHAAGGKQMMESALEGLEAGTPAGQKRPSLIAVTQLTSTSSEMLQRELLIETPLLDTVVHYSRLAEESGLDGVV
CSVHEAEHIYRAVSVDFLTVTPGIRMADDKNNDQVRVATPGYAREKGVSAIVVGRSITQAEDPVSAYRRIGHEWEGTKA
>Mature_239_residues
MNNTPPIIALDFASAQETYAFLDRFQGEELFVKVGMELFYQEGPAILENLQERGCRIFLDLKCHDIPTTVYKAMKRLAGF
GVSLVNVHAAGGKQMMESALEGLEAGTPAGQKRPSLIAVTQLTSTSSEMLQRELLIETPLLDTVVHYSRLAEESGLDGVV
CSVHEAEHIYRAVSVDFLTVTPGIRMADDKNNDQVRVATPGYAREKGVSAIVVGRSITQAEDPVSAYRRIGHEWEGTKA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=235, Percent_Identity=40, Blast_Score=161, Evalue=4e-41,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_BACLD (Q65JU4)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078948.1
- RefSeq:   YP_091363.1
- ProteinModelPortal:   Q65JU4
- SMR:   Q65JU4
- STRING:   Q65JU4
- EnsemblBacteria:   EBBACT00000058212
- EnsemblBacteria:   EBBACT00000059161
- GeneID:   3030930
- GeneID:   3101450
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01775
- KEGG:   bli:BL02279
- NMPDR:   fig|279010.5.peg.1717
- eggNOG:   COG0284
- GeneTree:   EBGT00050000000955
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   BLIC279010-1:BLI01775-MONOMER
- BioCyc:   BLIC279010:BL02279-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26213; Mature: 26213

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 11-11 BINDING 33-33 BINDING 123-123 BINDING 185-185 BINDING 194-194 BINDING 214-214 BINDING 215-215

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTPPIIALDFASAQETYAFLDRFQGEELFVKVGMELFYQEGPAILENLQERGCRIFLD
CCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEE
LKCHDIPTTVYKAMKRLAGFGVSLVNVHAAGGKQMMESALEGLEAGTPAGQKRPSLIAVT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
QLTSTSSEMLQRELLIETPLLDTVVHYSRLAEESGLDGVVCSVHEAEHIYRAVSVDFLTV
EECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHEEEEEE
TPGIRMADDKNNDQVRVATPGYAREKGVSAIVVGRSITQAEDPVSAYRRIGHEWEGTKA
CCCCEECCCCCCCEEEEECCCCHHHCCCEEEEECCCHHCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNNTPPIIALDFASAQETYAFLDRFQGEELFVKVGMELFYQEGPAILENLQERGCRIFLD
CCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEE
LKCHDIPTTVYKAMKRLAGFGVSLVNVHAAGGKQMMESALEGLEAGTPAGQKRPSLIAVT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
QLTSTSSEMLQRELLIETPLLDTVVHYSRLAEESGLDGVVCSVHEAEHIYRAVSVDFLTV
EECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHEEEEEE
TPGIRMADDKNNDQVRVATPGYAREKGVSAIVVGRSITQAEDPVSAYRRIGHEWEGTKA
CCCCEECCCCCCCEEEEECCCCHHHCCCEEEEECCCHHCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA