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The map label for this gene is pyrE

Identifier: 52785535

GI number: 52785535

Start: 1734782

End: 1735411

Strand: Direct

Name: pyrE

Synonym: BLi01776

Alternate gene names: 52785535

Gene position: 1734782-1735411 (Clockwise)

Preceding gene: 52785534

Following gene: 52785536

Centisome position: 41.08

GC content: 53.17

Gene sequence:

>630_bases
ATGAAAACAGACATTGCAAACCATTTATTAAAAATCAAAGCGGTTTTCCTGCGCCCGAACGAACCGTTTACATGGGCGAG
CGGCATTCTGTCGCCCATCTACTGCGACAACCGTTTGACGCTTTCATATCCGGAAGTAAGAGATGATATCGCTTCAGGGC
TTGCCGGACTGATTCAAGACAAATTTCCCGGAGCCGAGATGATCGCCGGTACAGCGACCGCGGGAATCCCTCATGCCGCG
CTTGCCGCTGACAGGCTGAGCCTGCCGATGTGCTACGTCAGAAGCAAACCGAAGGCGCACGGCAAAGGAAACCAAATTGA
AGGGGCCGTTGTAAAAGGACAGAAAGTCGTTGTGATCGAAGATTTAATTTCAACGGGAGGAAGCGTTCTTGAAGCCGCCG
CCGCCCTCACAGAGGCGGGATGTGAAGTGCTCGGCGTCGCCGCCATTTTCACTTACGGGCTTCCAAAAGCCGCCGCCGCC
TTCAGCGAAGCAGGCGTTGAATACCACACGCTGACTGATTACGATACGCTGACCGAGGCTGCGCTTGCCGGTGGATATAT
TACTGAACAGGATGCCGCCAAACTGAAAGAATGGAAAGCGGATCCTGAAAGCAAGTCTTGGATGAACTGA

Upstream 100 bases:

>100_bases
AAGGCGTGTCGGCGATCGTTGTCGGCCGTTCGATTACCCAGGCGGAAGATCCTGTGTCAGCCTACAGAAGAATAGGACAT
GAGTGGGAGGGAACCAAGGC

Downstream 100 bases:

>100_bases
TGATCAAAGCCGTATTCTCCAAATGGGAGAACGGCTTTTTTGCGCCTTTTTGCCGTTTTTAAAATAGTAAAATCGGATTA
TTTTTATTTTTTATTAATCC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKTDIANHLLKIKAVFLRPNEPFTWASGILSPIYCDNRLTLSYPEVRDDIASGLAGLIQDKFPGAEMIAGTATAGIPHAA
LAADRLSLPMCYVRSKPKAHGKGNQIEGAVVKGQKVVVIEDLISTGGSVLEAAAALTEAGCEVLGVAAIFTYGLPKAAAA
FSEAGVEYHTLTDYDTLTEAALAGGYITEQDAAKLKEWKADPESKSWMN

Sequences:

>Translated_209_residues
MKTDIANHLLKIKAVFLRPNEPFTWASGILSPIYCDNRLTLSYPEVRDDIASGLAGLIQDKFPGAEMIAGTATAGIPHAA
LAADRLSLPMCYVRSKPKAHGKGNQIEGAVVKGQKVVVIEDLISTGGSVLEAAAALTEAGCEVLGVAAIFTYGLPKAAAA
FSEAGVEYHTLTDYDTLTEAALAGGYITEQDAAKLKEWKADPESKSWMN
>Mature_209_residues
MKTDIANHLLKIKAVFLRPNEPFTWASGILSPIYCDNRLTLSYPEVRDDIASGLAGLIQDKFPGAEMIAGTATAGIPHAA
LAADRLSLPMCYVRSKPKAHGKGNQIEGAVVKGQKVVVIEDLISTGGSVLEAAAALTEAGCEVLGVAAIFTYGLPKAAAA
FSEAGVEYHTLTDYDTLTEAALAGGYITEQDAAKLKEWKADPESKSWMN

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=121, Percent_Identity=29.7520661157025, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508631, Length=201, Percent_Identity=25.8706467661692, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17554964, Length=191, Percent_Identity=26.7015706806283, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17933654, Length=118, Percent_Identity=30.5084745762712, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_BACLD (Q65JU3)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078949.1
- RefSeq:   YP_091364.1
- ProteinModelPortal:   Q65JU3
- SMR:   Q65JU3
- STRING:   Q65JU3
- EnsemblBacteria:   EBBACT00000057032
- EnsemblBacteria:   EBBACT00000060059
- GeneID:   3030931
- GeneID:   3098083
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01776
- KEGG:   bli:BL02280
- NMPDR:   fig|279010.5.peg.1718
- eggNOG:   COG0461
- GeneTree:   EBGT00050000002725
- HOGENOM:   HBG404341
- OMA:   LPMTYVR
- ProtClustDB:   PRK00455
- BioCyc:   BLIC279010-1:BLI01776-MONOMER
- BioCyc:   BLIC279010:BL02280-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 22109; Mature: 22109

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 94-94 BINDING 98-98 BINDING 100-100 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDIANHLLKIKAVFLRPNEPFTWASGILSPIYCDNRLTLSYPEVRDDIASGLAGLIQD
CCCHHHHHHHHHEEEEECCCCCCHHHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHHHC
KFPGAEMIAGTATAGIPHAALAADRLSLPMCYVRSKPKAHGKGNQIEGAVVKGQKVVVIE
CCCCHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEECCCEEEEEH
DLISTGGSVLEAAAALTEAGCEVLGVAAIFTYGLPKAAAAFSEAGVEYHTLTDYDTLTEA
HHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHHH
ALAGGYITEQDAAKLKEWKADPESKSWMN
HHHCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKTDIANHLLKIKAVFLRPNEPFTWASGILSPIYCDNRLTLSYPEVRDDIASGLAGLIQD
CCCHHHHHHHHHEEEEECCCCCCHHHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHHHC
KFPGAEMIAGTATAGIPHAALAADRLSLPMCYVRSKPKAHGKGNQIEGAVVKGQKVVVIE
CCCCHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEECCCEEEEEH
DLISTGGSVLEAAAALTEAGCEVLGVAAIFTYGLPKAAAAFSEAGVEYHTLTDYDTLTEA
HHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHHH
ALAGGYITEQDAAKLKEWKADPESKSWMN
HHHCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA