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The map label for this gene is tenI [H]

Identifier: 52785031

GI number: 52785031

Start: 1272068

End: 1272679

Strand: Direct

Name: tenI [H]

Synonym: BLi01260

Alternate gene names: 52785031

Gene position: 1272068-1272679 (Clockwise)

Preceding gene: 52785030

Following gene: 52785032

Centisome position: 30.12

GC content: 50.16

Gene sequence:

>612_bases
TTGCATCTGCACGCGATCACCGATGACAAGCACTCCGTGGAAGAGCTTTCAGCAAAAATCATCTCGATTCACGATGCCGT
TGACTTTATCCACATCAGGGAAAGGTCAAAAAAAGTAAGCGAAATATCAAGTTTGATCGATCGCCTGGCCGAAGAAGGCG
TTGATAAGCGAAAGCTCATCATCAACGACAGGGTGGATATTGCTTTGTTTCATCATATTCACCGGGTTCAGCTGCCTTCA
CACGGCTTTTCCGTCAAAAGTGTAAGAAGCCGTTTTCCGCATTTGAAAATCGGAAAGTCCGTACATTCACCTGAAGAAGC
GGTGCAGGCTGAAACTGAAGGTGCAGATTACGTGCTGTTCGGCCACATTTTTGAAACGGACTGTAAAAAGGGCCGGAAAG
GAAGAGGAGCTCTGAGCTTGGCAGAAGTGAAGGCGGCTGTCCGCATTCCGGTCATTGCGATCGGAGGGATTACCGAACAA
CGTCTGGCGGAAGTCAAAATGGCAGACGGCATCGCCGTGATGTCGGGGATCTTTTCCCATGACAGACCGAATGAGGCGGC
CGCGCGGCTTGCCAGTCTCGCGAAGGGAGATTCATATGAGAAAGCGCTATGA

Upstream 100 bases:

>100_bases
AAGATGAAGGAGAATTTCGTCATCTCCAGCTTCTACGAATACCGGTTCTGGGATATGGCCTATCAAAAAGAAAGCTGGTC
AAAAAGCGGGGTGGATCAGC

Downstream 100 bases:

>100_bases
TACGATCGTGATCGGCGGAGGCATCATCGGGACTTCCATCGCTTATCATCTTGCGAAAGCGGGGAAAAAAACGGCCGTGT
TTGAAAGCGGGGAAGTCGGC

Product: transcriptional regulator TenI

Products: Diphosphate; Thiamin monophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MHLHAITDDKHSVEELSAKIISIHDAVDFIHIRERSKKVSEISSLIDRLAEEGVDKRKLIINDRVDIALFHHIHRVQLPS
HGFSVKSVRSRFPHLKIGKSVHSPEEAVQAETEGADYVLFGHIFETDCKKGRKGRGALSLAEVKAAVRIPVIAIGGITEQ
RLAEVKMADGIAVMSGIFSHDRPNEAAARLASLAKGDSYEKAL

Sequences:

>Translated_203_residues
MHLHAITDDKHSVEELSAKIISIHDAVDFIHIRERSKKVSEISSLIDRLAEEGVDKRKLIINDRVDIALFHHIHRVQLPS
HGFSVKSVRSRFPHLKIGKSVHSPEEAVQAETEGADYVLFGHIFETDCKKGRKGRGALSLAEVKAAVRIPVIAIGGITEQ
RLAEVKMADGIAVMSGIFSHDRPNEAAARLASLAKGDSYEKAL
>Mature_203_residues
MHLHAITDDKHSVEELSAKIISIHDAVDFIHIRERSKKVSEISSLIDRLAEEGVDKRKLIINDRVDIALFHHIHRVQLPS
HGFSVKSVRSRFPHLKIGKSVHSPEEAVQAETEGADYVLFGHIFETDCKKGRKGRGALSLAEVKAAVRIPVIAIGGITEQ
RLAEVKMADGIAVMSGIFSHDRPNEAAARLASLAKGDSYEKAL

Specific function: Reduces the stimulatory effect of the tenA transcriptional activator on production of certain extracellular enzymes. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3 [C]

Molecular weight: Translated: 22376; Mature: 22376

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLHAITDDKHSVEELSAKIISIHDAVDFIHIRERSKKVSEISSLIDRLAEEGVDKRKLI
CEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
INDRVDIALFHHIHRVQLPSHGFSVKSVRSRFPHLKIGKSVHSPEEAVQAETEGADYVLF
ECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEE
GHIFETDCKKGRKGRGALSLAEVKAAVRIPVIAIGGITEQRLAEVKMADGIAVMSGIFSH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCHHHHHHHHCC
DRPNEAAARLASLAKGDSYEKAL
CCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHLHAITDDKHSVEELSAKIISIHDAVDFIHIRERSKKVSEISSLIDRLAEEGVDKRKLI
CEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
INDRVDIALFHHIHRVQLPSHGFSVKSVRSRFPHLKIGKSVHSPEEAVQAETEGADYVLF
ECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEE
GHIFETDCKKGRKGRGALSLAEVKAAVRIPVIAIGGITEQRLAEVKMADGIAVMSGIFSH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCHHHHHHHHCC
DRPNEAAARLASLAKGDSYEKAL
CCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0004 {thiazole} 0.00085 {hydroxymethylpyrimidine} [C]

Substrates: 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphoethyl)-thiazole; H+ [C]

Specific reaction: 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-Methyl-5-(2-phosphoethyl)-thiazole + H+ --> Diphosphate + Thiamin monophosphate [C]

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]