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The map label for this gene is tenA [H]

Identifier: 52785030

GI number: 52785030

Start: 1271392

End: 1272093

Strand: Direct

Name: tenA [H]

Synonym: BLi01259

Alternate gene names: 52785030

Gene position: 1271392-1272093 (Clockwise)

Preceding gene: 52785029

Following gene: 52785031

Centisome position: 30.11

GC content: 49.0

Gene sequence:

>702_bases
ATGAAGTTTTCAGAAGAATGCCGAAACAGCGCTGCAACTTGGTGGGAAGGGAGTTTTGTCCATCCGTTTATTCAAGGAAT
TGGAGACGGCACACTTCCGGTTGACCGATTTAAATACTATGTCATGCAGGATTCCTACTATTTGACCCACTTTGCCAAAG
TCCAATCCTTTGGAGCGGCATATGCTCACGATCTATATACGACAAGCCGGATGGCCGAACACGCGAAAGGGACATATGAA
GCCGAGATGTCGCTCCACAGGAAATTCACCGATCTATTGGGCATCACAAAGGAAGAGCTGGAGTCGTTTCAGCCGTCGCC
GACAGCGTATGCGTACACATCGCATTTGTACCGTTCTGTCGTCAGCGGCAGTTTTGCGGAAATACTCGCTGCTTTGCTTC
CTTGCTATTGGCTCTATTATGACGTCGGCGAAACGCTGAAAACGTGCAGGCCTGACAACGCGATTTATCAGGAATGGATC
GCGACATACGGAGGAGACTGGTTTAAAAAATCTGTAACGGAACAGATCAACCGCTTCGATGAGCTGGCTGAAAACGCCGA
TGAGAAAACGCGCGCGAAGATGAAGGAGAATTTCGTCATCTCCAGCTTCTACGAATACCGGTTCTGGGATATGGCCTATC
AAAAAGAAAGCTGGTCAAAAAGCGGGGTGGATCAGCTTGCATCTGCACGCGATCACCGATGA

Upstream 100 bases:

>100_bases
AGTGGCACGGTATTTGAGTATGTATATATGCAAATTCCAAACCACTTTCCTTGCGGGAAAGTGGTTTTTTTATGTTCAGA
ATCGAAGGAGGAGCCTTGAA

Downstream 100 bases:

>100_bases
CAAGCACTCCGTGGAAGAGCTTTCAGCAAAAATCATCTCGATTCACGATGCCGTTGACTTTATCCACATCAGGGAAAGGT
CAAAAAAAGTAAGCGAAATA

Product: TenA

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKFSEECRNSAATWWEGSFVHPFIQGIGDGTLPVDRFKYYVMQDSYYLTHFAKVQSFGAAYAHDLYTTSRMAEHAKGTYE
AEMSLHRKFTDLLGITKEELESFQPSPTAYAYTSHLYRSVVSGSFAEILAALLPCYWLYYDVGETLKTCRPDNAIYQEWI
ATYGGDWFKKSVTEQINRFDELAENADEKTRAKMKENFVISSFYEYRFWDMAYQKESWSKSGVDQLASARDHR

Sequences:

>Translated_233_residues
MKFSEECRNSAATWWEGSFVHPFIQGIGDGTLPVDRFKYYVMQDSYYLTHFAKVQSFGAAYAHDLYTTSRMAEHAKGTYE
AEMSLHRKFTDLLGITKEELESFQPSPTAYAYTSHLYRSVVSGSFAEILAALLPCYWLYYDVGETLKTCRPDNAIYQEWI
ATYGGDWFKKSVTEQINRFDELAENADEKTRAKMKENFVISSFYEYRFWDMAYQKESWSKSGVDQLASARDHR
>Mature_233_residues
MKFSEECRNSAATWWEGSFVHPFIQGIGDGTLPVDRFKYYVMQDSYYLTHFAKVQSFGAAYAHDLYTTSRMAEHAKGTYE
AEMSLHRKFTDLLGITKEELESFQPSPTAYAYTSHLYRSVVSGSFAEILAALLPCYWLYYDVGETLKTCRPDNAIYQEWI
ATYGGDWFKKSVTEQINRFDELAENADEKTRAKMKENFVISSFYEYRFWDMAYQKESWSKSGVDQLASARDHR

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=209, Percent_Identity=23.444976076555, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324997, Length=205, Percent_Identity=20.4878048780488, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 27116; Mature: 27116

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSEECRNSAATWWEGSFVHPFIQGIGDGTLPVDRFKYYVMQDSYYLTHFAKVQSFGAA
CCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
YAHDLYTTSRMAEHAKGTYEAEMSLHRKFTDLLGITKEELESFQPSPTAYAYTSHLYRSV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHH
VSGSFAEILAALLPCYWLYYDVGETLKTCRPDNAIYQEWIATYGGDWFKKSVTEQINRFD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ELAENADEKTRAKMKENFVISSFYEYRFWDMAYQKESWSKSGVDQLASARDHR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKFSEECRNSAATWWEGSFVHPFIQGIGDGTLPVDRFKYYVMQDSYYLTHFAKVQSFGAA
CCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
YAHDLYTTSRMAEHAKGTYEAEMSLHRKFTDLLGITKEELESFQPSPTAYAYTSHLYRSV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHH
VSGSFAEILAALLPCYWLYYDVGETLKTCRPDNAIYQEWIATYGGDWFKKSVTEQINRFD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ELAENADEKTRAKMKENFVISSFYEYRFWDMAYQKESWSKSGVDQLASARDHR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]