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The map label for this gene is cls [H]

Identifier: 52142360

GI number: 52142360

Start: 3011630

End: 3012856

Strand: Reverse

Name: cls [H]

Synonym: BCZK2883

Alternate gene names: 52142360

Gene position: 3012856-3011630 (Counterclockwise)

Preceding gene: 52142358

Following gene: 52142361

Centisome position: 56.84

GC content: 33.58

Gene sequence:

>1227_bases
GTGCTGAAAAAGATAAGAAGAATTTTCCAAGTGTTCCTCTTTTTATTTATCGGTGGATGTTTAGTTCTGTTTGTTTGGGG
TGAAATTGACAGAACGATAGCCGAAAAAAATGGGGTATTTAGCCAGAATTCTAGTCAATACCCTATGCGTCAAAGTGATA
TTACTTTGTATACAGATGGGAAACAGTTAAACAATCAACTATTCTCAGATATAGAGAAAGCAAAGCATTACGTGCATGTT
AACTTTTTTTCTATTGCTGATGATAAAGTAAGCCATCAGTTTCTAGAGTTATTGCGGAAAAAAAGTCAAGAAGGTGTAGA
AGTATACTATGCTTTGGACAGGTTAGGTGGAATTTTACTAAAGAAAAAGGAAAGAGAATTATTAGTTAAAAAAGGAGTTC
ATTTTACATATTACAATAAACCTGCTTTCCCATATCTTTTTTCTTCTTTGGATCATCGGAATCATCGTCGTATTTCAGTT
ATTGATGGAAAGGTTGGTTACATTGGTGGATTCAACATCGGAAAGAAGTATTTAGGAGAAAAAGAAAAACTTGGTTATTG
GAGAGATTATCATCTTCAAATACGAGGAGAAGGAGTTCAAGATTTAGAACACCAATTTGTATTAGACTGGAAGGGAAATT
CAGGGAAACCGATTCCAATACACAATGCGGTAAAGGAAAAGGGAGCAATCTCGCAACGATTTACTGCATATTATAGTGGA
GAAAAACTTGGGCAAGATTATAAAATGCTGTTTCAACAAGCAAAAGAATCCATTATTATATTAACACCTTATTTTATCCC
AAAGGACAGAACTATTTGGAACGCATTAGTAGATGCAAGAAAGAGGGGAGTCCGTGTCAAAATTCTATTCTCACCTCATT
CGGATGCACTATTAGTTAAAGAAGCAGCATATCCATATATCCGAAAAGCATTAAAACAGGGGATGGAAGTATATGGTTAT
AAAAATGGGGTGTTACACGGTAAAGTATTTTTGATTGATGAAAAAGTATTGATGGTTGGGACCGTGAATTTTGATTCACG
CTCGTTTCATTTAAATGAAGAAATGAACTGTTATATTCATGACCCTATCTATATTTCGAGAATAAAACCAGTGATTAATG
CAGACTTACAAAATTCAAAGCAAGTAACATCCTCTGACGTCAACCAATTATCTATAAAAGAAAAAATAAAAGAAAAGGTT
GCAAAAATGTTTGAGTATTATTTGTAA

Upstream 100 bases:

>100_bases
TCAAAGAAGGATTTTTTTAGCTAATAGATGCATGGAATAGTTTTGTCCCAATAACAAAAACTAGCAGAAATAAAACGATA
ATATATAAAGGAGATTAAAT

Downstream 100 bases:

>100_bases
AGACAATGTATTTTTATACTTGGATAACAGGGGCTATATGCTTAGTGATTTTACATATTTTCCATGGAAGCAGCTGGGCA
GTTGCTACAAGTTCGATAGA

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLKKIRRIFQVFLFLFIGGCLVLFVWGEIDRTIAEKNGVFSQNSSQYPMRQSDITLYTDGKQLNNQLFSDIEKAKHYVHV
NFFSIADDKVSHQFLELLRKKSQEGVEVYYALDRLGGILLKKKERELLVKKGVHFTYYNKPAFPYLFSSLDHRNHRRISV
IDGKVGYIGGFNIGKKYLGEKEKLGYWRDYHLQIRGEGVQDLEHQFVLDWKGNSGKPIPIHNAVKEKGAISQRFTAYYSG
EKLGQDYKMLFQQAKESIIILTPYFIPKDRTIWNALVDARKRGVRVKILFSPHSDALLVKEAAYPYIRKALKQGMEVYGY
KNGVLHGKVFLIDEKVLMVGTVNFDSRSFHLNEEMNCYIHDPIYISRIKPVINADLQNSKQVTSSDVNQLSIKEKIKEKV
AKMFEYYL

Sequences:

>Translated_408_residues
MLKKIRRIFQVFLFLFIGGCLVLFVWGEIDRTIAEKNGVFSQNSSQYPMRQSDITLYTDGKQLNNQLFSDIEKAKHYVHV
NFFSIADDKVSHQFLELLRKKSQEGVEVYYALDRLGGILLKKKERELLVKKGVHFTYYNKPAFPYLFSSLDHRNHRRISV
IDGKVGYIGGFNIGKKYLGEKEKLGYWRDYHLQIRGEGVQDLEHQFVLDWKGNSGKPIPIHNAVKEKGAISQRFTAYYSG
EKLGQDYKMLFQQAKESIIILTPYFIPKDRTIWNALVDARKRGVRVKILFSPHSDALLVKEAAYPYIRKALKQGMEVYGY
KNGVLHGKVFLIDEKVLMVGTVNFDSRSFHLNEEMNCYIHDPIYISRIKPVINADLQNSKQVTSSDVNQLSIKEKIKEKV
AKMFEYYL
>Mature_408_residues
MLKKIRRIFQVFLFLFIGGCLVLFVWGEIDRTIAEKNGVFSQNSSQYPMRQSDITLYTDGKQLNNQLFSDIEKAKHYVHV
NFFSIADDKVSHQFLELLRKKSQEGVEVYYALDRLGGILLKKKERELLVKKGVHFTYYNKPAFPYLFSSLDHRNHRRISV
IDGKVGYIGGFNIGKKYLGEKEKLGYWRDYHLQIRGEGVQDLEHQFVLDWKGNSGKPIPIHNAVKEKGAISQRFTAYYSG
EKLGQDYKMLFQQAKESIIILTPYFIPKDRTIWNALVDARKRGVRVKILFSPHSDALLVKEAAYPYIRKALKQGMEVYGY
KNGVLHGKVFLIDEKVLMVGTVNFDSRSFHLNEEMNCYIHDPIYISRIKPVINADLQNSKQVTSSDVNQLSIKEKIKEKV
AKMFEYYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=366, Percent_Identity=25.9562841530055, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787007, Length=340, Percent_Identity=28.8235294117647, Blast_Score=114, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 47422; Mature: 47422

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKIRRIFQVFLFLFIGGCLVLFVWGEIDRTIAEKNGVFSQNSSQYPMRQSDITLYTDG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
KQLNNQLFSDIEKAKHYVHVNFFSIADDKVSHQFLELLRKKSQEGVEVYYALDRLGGILL
HHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCEE
KKKERELLVKKGVHFTYYNKPAFPYLFSSLDHRNHRRISVIDGKVGYIGGFNIGKKYLGE
CCHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCHHHCCC
KEKLGYWRDYHLQIRGEGVQDLEHQFVLDWKGNSGKPIPIHNAVKEKGAISQRFTAYYSG
HHHCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHEECC
EKLGQDYKMLFQQAKESIIILTPYFIPKDRTIWNALVDARKRGVRVKILFSPHSDALLVK
HHHCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEE
EAAYPYIRKALKQGMEVYGYKNGVLHGKVFLIDEKVLMVGTVNFDSRSFHLNEEMNCYIH
HHCCHHHHHHHHCCHHHCCCCCCEEEEEEEEEECCEEEEEEECCCCCEEEECCCCCEEEE
DPIYISRIKPVINADLQNSKQVTSSDVNQLSIKEKIKEKVAKMFEYYL
CCEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKKIRRIFQVFLFLFIGGCLVLFVWGEIDRTIAEKNGVFSQNSSQYPMRQSDITLYTDG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
KQLNNQLFSDIEKAKHYVHVNFFSIADDKVSHQFLELLRKKSQEGVEVYYALDRLGGILL
HHHHHHHHHHHHHCCEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCEE
KKKERELLVKKGVHFTYYNKPAFPYLFSSLDHRNHRRISVIDGKVGYIGGFNIGKKYLGE
CCHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCHHHCCC
KEKLGYWRDYHLQIRGEGVQDLEHQFVLDWKGNSGKPIPIHNAVKEKGAISQRFTAYYSG
HHHCCCEEEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHEECC
EKLGQDYKMLFQQAKESIIILTPYFIPKDRTIWNALVDARKRGVRVKILFSPHSDALLVK
HHHCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEE
EAAYPYIRKALKQGMEVYGYKNGVLHGKVFLIDEKVLMVGTVNFDSRSFHLNEEMNCYIH
HHCCHHHHHHHHCCHHHCCCCCCEEEEEEEEEECCEEEEEEECCCCCEEEECCCCCEEEE
DPIYISRIKPVINADLQNSKQVTSSDVNQLSIKEKIKEKVAKMFEYYL
CCEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]