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The map label for this gene is oppA [H]

Identifier: 52141950

GI number: 52141950

Start: 3425441

End: 3427144

Strand: Reverse

Name: oppA [H]

Synonym: BCZK3293

Alternate gene names: 52141950

Gene position: 3427144-3425441 (Counterclockwise)

Preceding gene: 52141949

Following gene: 52141951

Centisome position: 64.65

GC content: 34.57

Gene sequence:

>1704_bases
GTGAAAAAGAAAAAAATGAAAAAACTAACAGCAGTTGTAGCACCAGTTTTAGCAATGAGTATGGCATTAACAGCATGTTC
TACATCAGGCGGAGATAAGAAGACAAGTACAAATTCTAGCTCTGGCAGAGATAGCAAATCAGAAGAAAAATTAGCAGCGA
AACAAGTGTTCAATAAAACAGAAAATCAGGAAATTCCAACAATGGATACGTCGAAGAGTACAGATACGTTGGGCTCTCAA
ATTTTAGGGAACACAATGGAAGGCTTATACCGTTTAGATAAAGAGAATAAACCAATCCCAGCTGCTGCAGAATCAAGCAC
GAAGAGTGAGGATGGTAAAAAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCAA
AAGATTTCGTATATGCATGGCAACGTCTATTAGATCCAAAAACAGCTGCTGAGTATGCATTTATCGCATTCCCAATTAAA
AATGCAGAAGCGGTTAATAAAGGTGAAAAACCTGTAGCAGAATTAGGAGTAAAGGCAGTAGACGACCTTACTTTAGAAGT
TGAATTAGAACAAGCAGTACCATACTTCTTAAACTTAGTAGCATTCCCATCATACTATCCGTTAAATGAGAAATTCGTAA
AAGAAAAAGGGGATAAATACGGTTTAGAATCTGATACAACTGTATATAACGGACCGTTCGTTCTTACTGATTGGAAGCAT
GAGCAAGGTTGGAAACTGAAGAAGAATGATCAATATTGGGATAAAAAGACTGTAAAATTAGATGAAATTAACTATAGTGT
TGTAAAAGAGCCAGCAACTAATGTGAATTTATATGATAGTGGTCAAATTGATTTCTCCTTATTAACAGGTGAATTTGTTG
ACAAATACAGAAATAATAAAGAGGAATATGGGGTGTATAACGAACCAAGTACGTTCTTTATACGTTTAAACCAAAAACGT
GGTGGACAAGATACACCATTAAAGAGTAAAAAATTACGTGAAGCAATTGCGCTATCAATTGATAAAAAGAATTTAACAAA
TGTTATTTTAAATGATGGGTCAAAACCTGCAGATTATTTAGTGCCGAAAGGCTTAGCAGCTGGACCTGATGGGAAAGACT
TCCAAGAAACGTTTAAAAACGGTGTTAAACCAGATGCGAAAAAAGCAGCTGCTGCATGGGAAGAAGCGAAAAAAGAACTT
GGAAAAGATCAAGTTACAATTGAGTTTTTAAACTATGATACTGGTAATGCGAAAAAAGTTGGGGAATATGTAAAAGATCA
AATTGAGAAAAACTTAAAAGGTGTAACAGTAAATATTAAACTGCAGCCGTTTAAACAAAAACTTAAATTAGAATCAGATC
AAGATTATGATATTTCATATGGTGGATGGAGCCCAGATTATGCGGATCCAATGACATATTTAGATATGTTTGAAAGTAAG
CACTCTCATAACCAAATGAGTTTCTCTGATCAGAAATATGATGAAATAATTAAAAAAGCTGGTGGGGAATTAATGAGTGA
TGCGAAGAAACGCTGGGAGGAACTAGGAAAAGCAGAGAAATTACTTCTTGAAGAAGATGTAGCCCTTGTACCTTTATATC
AAAGTGCTAGATCTTATGTTATGAAACCACATGTAAAAGGTGTTGTTAAGCATAATATTAGCCCAGAATACAGCTATAAG
TGGGCATATGTTACTGAAAAATAA

Upstream 100 bases:

>100_bases
ATAATAGTTAATAAGAATCTTCTGATAAATATATAAAGAAAAACAGAATTATCAGAAAATTCTTAAATGAGAGCGTTAAG
AATTAGGGAGGGTACAGAAA

Downstream 100 bases:

>100_bases
TAGAGTTGAAAAAGTACTTCAAAGCAGTCTACATGCCTTGAAGTACTTTTTTTGTTGTACAATTGAACGTGAAAATGAAA
GAGAAGAGAAGAGAAGAAAT

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MKKKKMKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGRDSKSEEKLAAKQVFNKTENQEIPTMDTSKSTDTLGSQ
ILGNTMEGLYRLDKENKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIK
NAEAVNKGEKPVAELGVKAVDDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVKEKGDKYGLESDTTVYNGPFVLTDWKH
EQGWKLKKNDQYWDKKTVKLDEINYSVVKEPATNVNLYDSGQIDFSLLTGEFVDKYRNNKEEYGVYNEPSTFFIRLNQKR
GGQDTPLKSKKLREAIALSIDKKNLTNVILNDGSKPADYLVPKGLAAGPDGKDFQETFKNGVKPDAKKAAAAWEEAKKEL
GKDQVTIEFLNYDTGNAKKVGEYVKDQIEKNLKGVTVNIKLQPFKQKLKLESDQDYDISYGGWSPDYADPMTYLDMFESK
HSHNQMSFSDQKYDEIIKKAGGELMSDAKKRWEELGKAEKLLLEEDVALVPLYQSARSYVMKPHVKGVVKHNISPEYSYK
WAYVTEK

Sequences:

>Translated_567_residues
MKKKKMKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGRDSKSEEKLAAKQVFNKTENQEIPTMDTSKSTDTLGSQ
ILGNTMEGLYRLDKENKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIK
NAEAVNKGEKPVAELGVKAVDDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVKEKGDKYGLESDTTVYNGPFVLTDWKH
EQGWKLKKNDQYWDKKTVKLDEINYSVVKEPATNVNLYDSGQIDFSLLTGEFVDKYRNNKEEYGVYNEPSTFFIRLNQKR
GGQDTPLKSKKLREAIALSIDKKNLTNVILNDGSKPADYLVPKGLAAGPDGKDFQETFKNGVKPDAKKAAAAWEEAKKEL
GKDQVTIEFLNYDTGNAKKVGEYVKDQIEKNLKGVTVNIKLQPFKQKLKLESDQDYDISYGGWSPDYADPMTYLDMFESK
HSHNQMSFSDQKYDEIIKKAGGELMSDAKKRWEELGKAEKLLLEEDVALVPLYQSARSYVMKPHVKGVVKHNISPEYSYK
WAYVTEK
>Mature_567_residues
MKKKKMKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGRDSKSEEKLAAKQVFNKTENQEIPTMDTSKSTDTLGSQ
ILGNTMEGLYRLDKENKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIK
NAEAVNKGEKPVAELGVKAVDDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVKEKGDKYGLESDTTVYNGPFVLTDWKH
EQGWKLKKNDQYWDKKTVKLDEINYSVVKEPATNVNLYDSGQIDFSLLTGEFVDKYRNNKEEYGVYNEPSTFFIRLNQKR
GGQDTPLKSKKLREAIALSIDKKNLTNVILNDGSKPADYLVPKGLAAGPDGKDFQETFKNGVKPDAKKAAAAWEEAKKEL
GKDQVTIEFLNYDTGNAKKVGEYVKDQIEKNLKGVTVNIKLQPFKQKLKLESDQDYDISYGGWSPDYADPMTYLDMFESK
HSHNQMSFSDQKYDEIIKKAGGELMSDAKKRWEELGKAEKLLLEEDVALVPLYQSARSYVMKPHVKGVVKHNISPEYSYK
WAYVTEK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=553, Percent_Identity=30.5605786618445, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87081878, Length=542, Percent_Identity=28.9667896678967, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789397, Length=518, Percent_Identity=28.7644787644788, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1787762, Length=458, Percent_Identity=26.2008733624454, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1787052, Length=514, Percent_Identity=23.1517509727626, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789887, Length=518, Percent_Identity=22.972972972973, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63926; Mature: 63926

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKKMKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGRDSKSEEKLAAKQVFNKT
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
ENQEIPTMDTSKSTDTLGSQILGNTMEGLYRLDKENKPIPAAAESSTKSEDGKKYTFKLR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEE
KDAKWSNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIKNAEAVNKGEKPVAELGVKAV
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCEEEEEEECCCCHHHCCCCCCHHHHCCHHH
DDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVKEKGDKYGLESDTTVYNGPFVLTDWKH
CCEEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEEEECCCC
EQGWKLKKNDQYWDKKTVKLDEINYSVVKEPATNVNLYDSGQIDFSLLTGEFVDKYRNNK
CCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCCEEEECCCEEEEEHHHHHHHHHHCCH
EEYGVYNEPSTFFIRLNQKRGGQDTPLKSKKLREAIALSIDKKNLTNVILNDGSKPADYL
HHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHH
VPKGLAAGPDGKDFQETFKNGVKPDAKKAAAAWEEAKKELGKDQVTIEFLNYDTGNAKKV
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHH
GEYVKDQIEKNLKGVTVNIKLQPFKQKLKLESDQDYDISYGGWSPDYADPMTYLDMFESK
HHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHH
HSHNQMSFSDQKYDEIIKKAGGELMSDAKKRWEELGKAEKLLLEEDVALVPLYQSARSYV
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEHHHHHHHH
MKPHVKGVVKHNISPEYSYKWAYVTEK
CCCHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKKKKMKKLTAVVAPVLAMSMALTACSTSGGDKKTSTNSSSGRDSKSEEKLAAKQVFNKT
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
ENQEIPTMDTSKSTDTLGSQILGNTMEGLYRLDKENKPIPAAAESSTKSEDGKKYTFKLR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEE
KDAKWSNGDPVTAKDFVYAWQRLLDPKTAAEYAFIAFPIKNAEAVNKGEKPVAELGVKAV
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCEEEEEEECCCCHHHCCCCCCHHHHCCHHH
DDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVKEKGDKYGLESDTTVYNGPFVLTDWKH
CCEEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEEEECCCC
EQGWKLKKNDQYWDKKTVKLDEINYSVVKEPATNVNLYDSGQIDFSLLTGEFVDKYRNNK
CCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCCEEEECCCEEEEEHHHHHHHHHHCCH
EEYGVYNEPSTFFIRLNQKRGGQDTPLKSKKLREAIALSIDKKNLTNVILNDGSKPADYL
HHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHH
VPKGLAAGPDGKDFQETFKNGVKPDAKKAAAAWEEAKKELGKDQVTIEFLNYDTGNAKKV
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHH
GEYVKDQIEKNLKGVTVNIKLQPFKQKLKLESDQDYDISYGGWSPDYADPMTYLDMFESK
HHHHHHHHHCCCCEEEEEEEECCHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHH
HSHNQMSFSDQKYDEIIKKAGGELMSDAKKRWEELGKAEKLLLEEDVALVPLYQSARSYV
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEHHHHHHHH
MKPHVKGVVKHNISPEYSYKWAYVTEK
CCCHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]