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The map label for this gene is dctA [H]

Identifier: 52141949

GI number: 52141949

Start: 3427492

End: 3428754

Strand: Reverse

Name: dctA [H]

Synonym: BCZK3294

Alternate gene names: 52141949

Gene position: 3428754-3427492 (Counterclockwise)

Preceding gene: 52141948

Following gene: 52141950

Centisome position: 64.68

GC content: 35.39

Gene sequence:

>1263_bases
ATGAGAATCGTAAAGAATTTAACATTTCAAGTTATTGTGGCCATTATTTGTGGTATTGCAGTAGGAGCAATTTGGCCGAG
TGTTGGACAAGAGATGAAGCCAATTGGTGAAACGTTCATCAATATGATTAAAATGGTTATTGCACCAATTATCTTTTTAA
CAATTGTGCTTGGTATTGCAAGTATGGGAAGTATGAAAAAGGTAGGCCGTGTTGGCGGGAAGGCACTATTATATTTTGAA
ATTGTTACAACAGCGGCTTTAATAATTGGTATCATTGTAGCGAATGTTGTGCGTCCAGGAGACGGATTAGACCCTTCAAA
ACTAAAGGGCGGAGATGTTTCACAATATGTACAAAGCGGGCAAGAAATGAAATGGATGGATTTCTTTTTACATATTGTGC
CGTCTAACATGTTTGAAGCATTTGCAAAAGGTGATATTTTACAAGTGTTATTTTTCTCTATTTTATTTGGCGCAGGGTTA
ACGATGTTAGGGAAAAATGGACAGCCGGTAATCGACTTTTTTGAAAGATTATCGAAAGTATTTTTTAACATTTTATCAAT
TGTAATGAAATTAGCACCGGTTGGAGCATTCGGTGGAATGGCATTTACAATTGGAAAATACGGTTTAAGCACGCTCATAC
CACTTGGTAAACTAATGATTTGTGTATATGCGACAATGGCATTATTTGTTTTTATTGTTTTAAATTTCATTTGTAAATTG
TACAAATTTAGTTTGTGGAAATATTTATCTCACATTAAAGAAGAATTACTTATTGTTCTTGGGACATCATCATCAGAATC
AGTATTGCCGCGAATGATGACAAAGATGGAAGACTTCGGATGTTCGAAGCCGGTAGTAGGTTTAGTCATTCCGACGGGAT
ACTCTTTTAACTTAGATGGAACAACAATTTATTTATCCATGGCTACTATATTTTTAGCACAAGTCTTTCACGTCGATCTT
TCACTAGGTCAACAATTAACTATAATAGCTATTTTATTAGTTACATCTAAAGGAGCAGCGGCAGTAACAGGCGGGGGATT
TATTGTACTAGCATCTACTCTATCGGCAATGAATGTTATTCCGTTAGAAGGGTTAGCGCTATTACTTGGTGTAGATCGTT
TCATGTCAGAAGCACGTGCTATCGTTAATTTAATTGGTAATGGTATTGCGACTGTAGTAGTTGCAAAAAGTGAAAATGAA
TTTGATAATGAGAAGTATATGAAAGTACTAGAAGAAATGAAAAGAGAGAAAATGGCCGGATAA

Upstream 100 bases:

>100_bases
AAGTTAAGGTTTAAGTTACATAACAGAGGGCCATCACATAGAATAAATGTTATTGATGAAAATAGTTTGGGAGAAATGAA
AAAATAAGGGAGTATTAAAA

Downstream 100 bases:

>100_bases
AAAGTCAGCTTTGCTGACTTTTTTATTTTTGTAGAAAAGAGGTTTGCTATAATATACATTATTTATTAACAATAATTTAC
AGGTTTTTTGTAGAGAAGGG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLSHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDNEKYMKVLEEMKREKMAG

Sequences:

>Translated_420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLSHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDNEKYMKVLEEMKREKMAG
>Mature_420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQEMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFAKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLSHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDNEKYMKVLEEMKREKMAG

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=449, Percent_Identity=24.7216035634744, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21314632, Length=431, Percent_Identity=25.9860788863109, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI40254478, Length=475, Percent_Identity=24.2105263157895, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI5032093, Length=421, Percent_Identity=26.1282660332542, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI223468564, Length=283, Percent_Identity=28.6219081272085, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI66773030, Length=421, Percent_Identity=26.1282660332542, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI223468566, Length=283, Percent_Identity=28.6219081272085, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI194239697, Length=432, Percent_Identity=24.537037037037, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4827012, Length=270, Percent_Identity=27.4074074074074, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=24.2021276595745, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI301601644, Length=174, Percent_Identity=28.735632183908, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789947, Length=410, Percent_Identity=55.8536585365854, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1790514, Length=430, Percent_Identity=40.4651162790698, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1789473, Length=319, Percent_Identity=22.884012539185, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=425, Percent_Identity=26.8235294117647, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71983099, Length=422, Percent_Identity=25.1184834123223, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983106, Length=422, Percent_Identity=25.1184834123223, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193206505, Length=406, Percent_Identity=26.8472906403941, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=413, Percent_Identity=25.90799031477, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=437, Percent_Identity=24.9427917620137, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=436, Percent_Identity=23.6238532110092, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24583025, Length=400, Percent_Identity=24.25, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17137668, Length=400, Percent_Identity=24.25, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583023, Length=400, Percent_Identity=24.25, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281360481, Length=419, Percent_Identity=23.8663484486874, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281360483, Length=419, Percent_Identity=23.8663484486874, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137666, Length=419, Percent_Identity=23.8663484486874, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45526; Mature: 45526

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure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PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]