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The map label for this gene is oppA [H]

Identifier: 52141951

GI number: 52141951

Start: 3423422

End: 3425128

Strand: Reverse

Name: oppA [H]

Synonym: BCZK3292

Alternate gene names: 52141951

Gene position: 3425128-3423422 (Counterclockwise)

Preceding gene: 52141950

Following gene: 52141953

Centisome position: 64.61

GC content: 35.44

Gene sequence:

>1707_bases
ATGAAGAAAAAGATGAAGAAGTTCACGGCGGTTGTAGCGCCAGTTTTAGCGATGAGTATGGCGTTGACAGCTTGTTCTGG
ATCTGGTGGGGAGAAGAAATCAACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAATCTGAAATTAAATATGCAGCAA
AACAAGTGTTAAATCGTACAGAGAATCAAGAGATTCCGACGATGGATACTTCTAAATCTACTGATACGCTAGGGGCACAA
ATTTTAGGAAACACGATGGAAGGGTTATATCGTCTTGATAAAGATAATAAGCCAATTCCTGCTGCTGCAGAATCTAGTAC
GAAAAGCGAGGATGGCAAAAAATATACATTTAAATTACGTAAAGATGCAAAATGGTCGAATGGTGATCCTGTAACAGCGA
AAGATTTCGTATACGGATGGCAACGCTTACTCGATAAAAATACTGCTGCAGAATATGCGTTTATTGCTTTCTATATTAAA
AATGGAGAAGCAATTAATAAAGGTGAAAAACCTCTAACAGATTTAGGAGCAAAAGCAGTAGATGATTATACGCTAGAAGT
AGAATTAGAAAAGCCAGTACCATATTTCTTAAATTTACTAGCATTCCCGTCATACTATCCTTTAAATGAGAAATTCGTAA
AAGAAAAAGGAGATAAGTTCGGTTTAGAAGCAGATACAACTTTATATAATGGACCGTTTGTGATGGCTTCATGGAAACAT
GAACAAGGATGGCAGCTAAAGAAAAATGATAAGTACTGGGATAATAAGACTGTAAAATTAGAAGAAATTAACTATAGTGT
AGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGATTTTACATTATTATCAGGAGAATTCGTTG
ATAAATATAAATCAAACAAAGAGGAGTACGGTGAGTATTCAGAAGCGAGTACATTCTTCTTACGTTTAAATCAAAAACGT
AACGGACAAGATACACCGTTAAAGAGCAAAAAACTTCGTGAAGCGATCGCATTATCAATTGATAAAAAAGGATTAGCAAA
CGTTATTTTAAATAACGGTTCAAAAGCAACAGATCAATTAGTACCAAAAGGGCTTGCGACAGGACCAGACGGTAAAGACT
ACCAAGATACGTTTAAAAATGGTCTAAAATATGATCCAAAAAAAGGTGCAGCAGCTTGGGAAGCAGCGAAAAAAGAACTT
GGTAAAGATCAAGTGACAATTGAATTACTAAGCTATGATGATGGAACTGCGAAAAAAATTGCTGACTACTTTAAAGATCA
AATTGAGAAAAACTTAAAAGGTGTAACGGTTAACACGAAAATTCAACCGTTCAAACAAAAACTAAAATTAGAGTCAGCAC
AAGATTATGAAGTTTCGTTTGCAGGTTGGAGCCCAGATTATTCGGATCCAATGACATTTATTGATATGTTTGAATCGAAG
AGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTAGCGAAAGCAGGTAATGAATTACTGTCTGA
TCCGAAGAAGCGTTGGGAAACGTTAGGAAAAGCAGAGAAATTATTCCTTGAAGAAGATGCAGGATTAGTTCCTTTATATC
AAACAGGAAGAGCATATGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATTAGTCCAGAATATAGCTTTAAG
TGGGCGTATGTAACGGAAGGGAAATAA

Upstream 100 bases:

>100_bases
AAAATTAGTAGAATATTAATTAATAAGAATTTTCTGATAAATAAACATTAGAAAATCTCTCAAAATTAGTTTCGTACATA
TAATTGGGGAGGGTACAAAA

Downstream 100 bases:

>100_bases
GAAAAAAGAGCGTTTGAAAAATATATTATTTATTTTTTTAAAACGCTCTTTTGCTATCCAATTAAATTTAAATAGATAGA
GAAGAATAAGAAAAGTGGTA

Product: ABC transporter, substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDTSKSTDTLGAQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYGWQRLLDKNTAAEYAFIAFYIK
NGEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
EQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKR
NGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEAAKKEL
GKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESK
SPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYVMKPNVKGIVKHNISPEYSFK
WAYVTEGK

Sequences:

>Translated_568_residues
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDTSKSTDTLGAQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYGWQRLLDKNTAAEYAFIAFYIK
NGEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
EQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKR
NGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEAAKKEL
GKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESK
SPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYVMKPNVKGIVKHNISPEYSFK
WAYVTEGK
>Mature_568_residues
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDTSKSTDTLGAQ
ILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYGWQRLLDKNTAAEYAFIAFYIK
NGEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
EQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKR
NGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEAAKKEL
GKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESK
SPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYVMKPNVKGIVKHNISPEYSFK
WAYVTEGK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=511, Percent_Identity=31.5068493150685, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1789397, Length=509, Percent_Identity=29.8624754420432, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=27.8504672897196, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787762, Length=455, Percent_Identity=27.9120879120879, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1787052, Length=513, Percent_Identity=23.9766081871345, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789887, Length=335, Percent_Identity=26.865671641791, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789966, Length=477, Percent_Identity=22.6415094339623, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63766; Mature: 63766

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
ENQEIPTMDTSKSTDTLGAQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
KDAKWSNGDPVTAKDFVYGWQRLLDKNTAAEYAFIAFYIKNGEAINKGEKPLTDLGAKAV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHCCHHC
DDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
CCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEEEEECCC
EQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNK
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEEECCCEEEEEECCHHHHHHHCCH
EEYGEYSEASTFFLRLNQKRNGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQL
HHHCCHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEECCCCHHHHHH
VPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEAAKKELGKDQVTIELLSYDDGTAKKI
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHH
ADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESK
HHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCC
SPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYV
CCCCCCCCCCCCHHHHHHHHCHHHHCCHHHHHHHHCHHHHHEEECCCCEEEEEECCCEEE
MKPNVKGIVKHNISPEYSFKWAYVTEGK
ECCCCCEEEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKKKMKKFTAVVAPVLAMSMALTACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
ENQEIPTMDTSKSTDTLGAQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
KDAKWSNGDPVTAKDFVYGWQRLLDKNTAAEYAFIAFYIKNGEAINKGEKPLTDLGAKAV
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHCCHHC
DDYTLEVELEKPVPYFLNLLAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKH
CCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEECCCEEEEECCC
EQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNK
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCEEECCCEEEEEECCHHHHHHHCCH
EEYGEYSEASTFFLRLNQKRNGQDTPLKSKKLREAIALSIDKKGLANVILNNGSKATDQL
HHHCCHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEECCCCHHHHHH
VPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEAAKKELGKDQVTIELLSYDDGTAKKI
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHH
ADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESK
HHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCC
SPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYV
CCCCCCCCCCCCHHHHHHHHCHHHHCCHHHHHHHHCHHHHHEEECCCCEEEEEECCCEEE
MKPNVKGIVKHNISPEYSFKWAYVTEGK
ECCCCCEEEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]