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The map label for this gene is ptsI [H]

Identifier: 51894413

GI number: 51894413

Start: 3504181

End: 3505902

Strand: Reverse

Name: ptsI [H]

Synonym: STH3279

Alternate gene names: 51894413

Gene position: 3505902-3504181 (Counterclockwise)

Preceding gene: 51894414

Following gene: 51894403

Centisome position: 98.31

GC content: 72.88

Gene sequence:

>1722_bases
TTGAGGGTGGCCTCGGTGAGTGAAAAGGTCCTGAACGGCGTCGCGGCCGCCGCGGGCGTCGTCATCGGTCCCGCGTTCGT
CTACCGGGAGGGGCAGCCGGAGACCGCGGCACGGGCGGCTGCTGCTCCGGAGGAGGAGCTGGCCCGGTTTGAGGAGGCCC
GCCGCCGCGCCGAGAATGAGCTCAACGCCCTGGCCGAGCGGGCCGACGACCAGGGCCGGGAGATCCTGACCGCGCACCAG
CTGATGCTCCAGGATCCGGAGCTGCAGAACCTGGTGGAGGCAGCCATCCGGGACGGGCAGCCAGCCGAGGAGGCCGTGCG
CACCGCCACGGAGCAGTTCGCCGCGATGCTGGAGGCCCTGGATGACGAGTATCTGCGGGAGCGCGCGGCCGACGTGCGCG
ACGTGGGCCGGCGGCTTGTCGCGGCCCTGACCGGCCGCACGGTGGGGGTCGTGCTGCAGCGGCCGTCGGTGGTCGTAGCG
CGGGACCTGGCCCCCACGGACACCATTGGCATCGACCGCTCGCTGCTCCTGGGCATCGTGACGGAGCAGGGCGGGCCCAC
CTCGCACACCACGATCCTGGCCCGCTCCTGGGGCATCCCCGCGGTGGTGGCAACGGCCGGCGTGCTCGAGGCCGCGTCCG
ACGGCATGACGGTCGCGCTGGACGGCGACGCGGGCGAGGTCGTTCTCGATCCGGGAGCGGAGACGCGCAGCCGCTACGAG
GCCGCCATGGCGCGCGCCCGCGAGCAGGCGGAACGCGACCGGGCCGAGGCGTCGCTGCCGGCCGAGACTCCGGACGGCGT
GCGGGTGGAGCTGGCGGGCAACGCCGGCTCGCCCGGCGAGGTGGCCTTCGCGATGGAGAAGGGCGCCGAGGGCATCGGCC
TCCTCCGCTCCGAGTTCCTCTTCATGGGGCGTTCCACCGCCCCCACGGAGGAGGAGCAGTATCAGGCCTACGCCGAGGCT
CTGCGGAACGCCCGCGGGCAGCGGGTGATCATCCGCACCCTGGATATCGGCGGTGACAAGGACGTGCCGTACCTGGGCCT
GGCGAAGGAGGAGAACCCCTTCCTGGGCGTCCGGGCCCTCAGGCTGTGCTTCCGCCGCCCCGAGCTCTTCCAGACGCAGT
TGCGGGCGCTGCTTCGGGCGTCGGTCCATGGCCGGCTGGCCATCATGTTCCCCATGGTGAGCGGCCTGAGCGACCTGCGC
CGGGCGAAGGAGGCCCTGGCGGAGGCCCGCCGCTCGCTGGAGGCCGAGGGTCATCCGGTGGCCGACCGGTACGAGGTCGG
CATCATGGTGGAGATCCCGTCCGCCGCTCTCCTGGCCGATCACCTGGCCCGGGAGGTGGACTTCTTCTCCATCGGCACCA
ACGACCTGGTGCAGTACACCCTGGCGGTGGACCGGGGCAACCCGGAGCTGACGGAGATGTACCAGCCCTACCATCCGGCC
GTCCTGCGGCTGATCGACCGCATCGTGCAGGCGGCGCACGCCGCCGGCAAGTGGGTCGGCGTGTGCGGCGAGATGGGTGG
GCTGCCGGAGGGTGCCCTGCTTCTGCTCGGCCTGGGCGTGGACGAGCTCTCGATGGCCCCTGCCCTGCTGCCTCGCATCA
AGCGGCTGGTGCGGTCCACGCCGGCCGCGGAGGCGCGGGCGGTGGCCCGGGAGTGCCTGGAGTTGGGCACGCCCGAGGAA
GTGCTGGCGCTGGTTGGGCCGCTGGTCCGGCGTGTCGGGTAG

Upstream 100 bases:

>100_bases
CTCTCCCTCCTGAGCCTCGGCGTCGCCCAGGGGGAGACCATCGTCATCCGCTGCCAGGGGCCCCGGGAAGAGGAGGCCAT
GGCGGCGCTGGCCGCGCTGA

Downstream 100 bases:

>100_bases
TCCAACGTCAAGAATGGCAAGGGATCGCCTCGGTCGGGGCGGTCCCTTTCTTGCTGTTCGAGCGGCTCCGCCGGCACTGC
GGCGACCGCCTCCGTCCGGG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MRVASVSEKVLNGVAAAAGVVIGPAFVYREGQPETAARAAAAPEEELARFEEARRRAENELNALAERADDQGREILTAHQ
LMLQDPELQNLVEAAIRDGQPAEEAVRTATEQFAAMLEALDDEYLRERAADVRDVGRRLVAALTGRTVGVVLQRPSVVVA
RDLAPTDTIGIDRSLLLGIVTEQGGPTSHTTILARSWGIPAVVATAGVLEAASDGMTVALDGDAGEVVLDPGAETRSRYE
AAMARAREQAERDRAEASLPAETPDGVRVELAGNAGSPGEVAFAMEKGAEGIGLLRSEFLFMGRSTAPTEEEQYQAYAEA
LRNARGQRVIIRTLDIGGDKDVPYLGLAKEENPFLGVRALRLCFRRPELFQTQLRALLRASVHGRLAIMFPMVSGLSDLR
RAKEALAEARRSLEAEGHPVADRYEVGIMVEIPSAALLADHLAREVDFFSIGTNDLVQYTLAVDRGNPELTEMYQPYHPA
VLRLIDRIVQAAHAAGKWVGVCGEMGGLPEGALLLLGLGVDELSMAPALLPRIKRLVRSTPAAEARAVARECLELGTPEE
VLALVGPLVRRVG

Sequences:

>Translated_573_residues
MRVASVSEKVLNGVAAAAGVVIGPAFVYREGQPETAARAAAAPEEELARFEEARRRAENELNALAERADDQGREILTAHQ
LMLQDPELQNLVEAAIRDGQPAEEAVRTATEQFAAMLEALDDEYLRERAADVRDVGRRLVAALTGRTVGVVLQRPSVVVA
RDLAPTDTIGIDRSLLLGIVTEQGGPTSHTTILARSWGIPAVVATAGVLEAASDGMTVALDGDAGEVVLDPGAETRSRYE
AAMARAREQAERDRAEASLPAETPDGVRVELAGNAGSPGEVAFAMEKGAEGIGLLRSEFLFMGRSTAPTEEEQYQAYAEA
LRNARGQRVIIRTLDIGGDKDVPYLGLAKEENPFLGVRALRLCFRRPELFQTQLRALLRASVHGRLAIMFPMVSGLSDLR
RAKEALAEARRSLEAEGHPVADRYEVGIMVEIPSAALLADHLAREVDFFSIGTNDLVQYTLAVDRGNPELTEMYQPYHPA
VLRLIDRIVQAAHAAGKWVGVCGEMGGLPEGALLLLGLGVDELSMAPALLPRIKRLVRSTPAAEARAVARECLELGTPEE
VLALVGPLVRRVG
>Mature_573_residues
MRVASVSEKVLNGVAAAAGVVIGPAFVYREGQPETAARAAAAPEEELARFEEARRRAENELNALAERADDQGREILTAHQ
LMLQDPELQNLVEAAIRDGQPAEEAVRTATEQFAAMLEALDDEYLRERAADVRDVGRRLVAALTGRTVGVVLQRPSVVVA
RDLAPTDTIGIDRSLLLGIVTEQGGPTSHTTILARSWGIPAVVATAGVLEAASDGMTVALDGDAGEVVLDPGAETRSRYE
AAMARAREQAERDRAEASLPAETPDGVRVELAGNAGSPGEVAFAMEKGAEGIGLLRSEFLFMGRSTAPTEEEQYQAYAEA
LRNARGQRVIIRTLDIGGDKDVPYLGLAKEENPFLGVRALRLCFRRPELFQTQLRALLRASVHGRLAIMFPMVSGLSDLR
RAKEALAEARRSLEAEGHPVADRYEVGIMVEIPSAALLADHLAREVDFFSIGTNDLVQYTLAVDRGNPELTEMYQPYHPA
VLRLIDRIVQAAHAAGKWVGVCGEMGGLPEGALLLLGLGVDELSMAPALLPRIKRLVRSTPAAEARAVARECLELGTPEE
VLALVGPLVRRVG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=41.3612565445026, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI48994992, Length=489, Percent_Identity=41.7177914110429, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788726, Length=568, Percent_Identity=37.1478873239437, Blast_Score=355, Evalue=6e-99,
Organism=Escherichia coli, GI1789193, Length=537, Percent_Identity=35.3817504655494, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1787994, Length=395, Percent_Identity=28.1012658227848, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI226510935, Length=207, Percent_Identity=27.536231884058, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 61575; Mature: 61575

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVASVSEKVLNGVAAAAGVVIGPAFVYREGQPETAARAAAAPEEELARFEEARRRAENE
CCCCHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
LNALAERADDQGREILTAHQLMLQDPELQNLVEAAIRDGQPAEEAVRTATEQFAAMLEAL
HHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DDEYLRERAADVRDVGRRLVAALTGRTVGVVLQRPSVVVARDLAPTDTIGIDRSLLLGIV
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHEE
TEQGGPTSHTTILARSWGIPAVVATAGVLEAASDGMTVALDGDAGEVVLDPGAETRSRYE
ECCCCCCCHHEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCHHHHHHH
AAMARAREQAERDRAEASLPAETPDGVRVELAGNAGSPGEVAFAMEKGAEGIGLLRSEFL
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEHHCCCCHHHHHHHHHH
FMGRSTAPTEEEQYQAYAEALRNARGQRVIIRTLDIGGDKDVPYLGLAKEENPFLGVRAL
HHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCCCCCCHHHHHH
RLCFRRPELFQTQLRALLRASVHGRLAIMFPMVSGLSDLRRAKEALAEARRSLEAEGHPV
HHHHCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ADRYEVGIMVEIPSAALLADHLAREVDFFSIGTNDLVQYTLAVDRGNPELTEMYQPYHPA
CCCEEEEEEEECCCHHHHHHHHHHHCCEEECCCHHHEEEEEEEECCCCHHHHHHCCCCHH
VLRLIDRIVQAAHAAGKWVGVCGEMGGLPEGALLLLGLGVDELSMAPALLPRIKRLVRST
HHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
PAAEARAVARECLELGTPEEVLALVGPLVRRVG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVASVSEKVLNGVAAAAGVVIGPAFVYREGQPETAARAAAAPEEELARFEEARRRAENE
CCCCHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
LNALAERADDQGREILTAHQLMLQDPELQNLVEAAIRDGQPAEEAVRTATEQFAAMLEAL
HHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DDEYLRERAADVRDVGRRLVAALTGRTVGVVLQRPSVVVARDLAPTDTIGIDRSLLLGIV
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHEE
TEQGGPTSHTTILARSWGIPAVVATAGVLEAASDGMTVALDGDAGEVVLDPGAETRSRYE
ECCCCCCCHHEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCHHHHHHH
AAMARAREQAERDRAEASLPAETPDGVRVELAGNAGSPGEVAFAMEKGAEGIGLLRSEFL
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEHHCCCCHHHHHHHHHH
FMGRSTAPTEEEQYQAYAEALRNARGQRVIIRTLDIGGDKDVPYLGLAKEENPFLGVRAL
HHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEECCCCCCCCHHHHHH
RLCFRRPELFQTQLRALLRASVHGRLAIMFPMVSGLSDLRRAKEALAEARRSLEAEGHPV
HHHHCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ADRYEVGIMVEIPSAALLADHLAREVDFFSIGTNDLVQYTLAVDRGNPELTEMYQPYHPA
CCCEEEEEEEECCCHHHHHHHHHHHCCEEECCCHHHEEEEEEEECCCCHHHHHHCCCCHH
VLRLIDRIVQAAHAAGKWVGVCGEMGGLPEGALLLLGLGVDELSMAPALLPRIKRLVRST
HHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
PAAEARAVARECLELGTPEEVLALVGPLVRRVG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA