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The map label for this gene is ispE

Identifier: 51894381

GI number: 51894381

Start: 3460585

End: 3461433

Strand: Reverse

Name: ispE

Synonym: STH3246

Alternate gene names: 51894381

Gene position: 3461433-3460585 (Counterclockwise)

Preceding gene: 51894382

Following gene: 51894380

Centisome position: 97.06

GC content: 73.14

Gene sequence:

>849_bases
ATGGAAATCGAATGCCACGCAAAGGTGAACCTCACGCTGGACGTGCTGGGTCGGCGGCCGGACGGATACCACGAGATCCG
CTCCGTCATGGTACCCGTTTCTCTCCACGACCGCCTGGTCCTCGAGCCGGCGGAGGAGGAGATCGTCCTGGAAAGCCGGC
CCCCCGCAACCGACCGGTTGGAGGAGAACCTGGCCTACCGGGCCGCGGCGCTGCTCCGGGAGGCGACGGGATGCAGCCGG
GGCGCGGTCATCCGGCTGCAGAAAACCATCCCGGTGGCTGCCGGCCTGGCCGGCGGCTCCACCGACGCGGCGGGCGTCCT
GACGGGGCTCAACCGTCTGTGGGGGACGGGGCTCTCCGACGGGGAGCTGGCGGACCTGGCCATCCGGCTCGGTTCCGACG
TGCCGTTCTTCATCTGGTCCCGCCCCGCCCGGGTGGAGGGGATCGGCGAGCGGGTCACCCCGCTCCCCGTGGCCGGGCCG
CTCTGGATGGTGGTGGCCACGCCGGACGTCCCCAAGTCCACCGGCCAGGTGTACCGGTGGTTCGATGAGCTGGCGGACGT
GGGGCCGCGCCCCGACGCCGGGGCGATGGAAGCGGCCCTGGCCCGGGGGGACGCCGCGGCGGTGGGCCGCGCCCTCTGCA
ACGTGTTCGAACAGGTCATGCTGCCGCGCCACCCGGAGATCGCCCGGCTCAAGGAGGCGATGCTGGCCGCCGGCGCGCTG
GGGGCGGTCATGTCGGGGGCGGGTCCCTCGGTGCTGGGCGTGGTTCCGGACCGGGAGGCGGGCGGTAGGCTGCTGGAGCG
CATCCGGCCGCTTTCCCGGGACGCCTGGGTGGTCCGTACCCTGGTCTGA

Upstream 100 bases:

>100_bases
GCGGCACGGAGACCAAGTTCGAGGCGCATTCCCGCTAGGCGACCCGAGACAAGAGCCAGGGAGAATCCCTGGCTTTTTCG
GTGTGTGGAGGGAAATCCGG

Downstream 100 bases:

>100_bases
GGGTTCTGGGCGACGCCGGCAGGAACTCCGTCGGCGCGCCGAGAAGCAAACTGTTTGTTTCGTCCATAAACTTCTGGGCA
TCGAGGAGAGATCGGGCATG

Product: putative 4-diphosphocytidyl-2-C-methyl-D- erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MEIECHAKVNLTLDVLGRRPDGYHEIRSVMVPVSLHDRLVLEPAEEEIVLESRPPATDRLEENLAYRAAALLREATGCSR
GAVIRLQKTIPVAAGLAGGSTDAAGVLTGLNRLWGTGLSDGELADLAIRLGSDVPFFIWSRPARVEGIGERVTPLPVAGP
LWMVVATPDVPKSTGQVYRWFDELADVGPRPDAGAMEAALARGDAAAVGRALCNVFEQVMLPRHPEIARLKEAMLAAGAL
GAVMSGAGPSVLGVVPDREAGGRLLERIRPLSRDAWVVRTLV

Sequences:

>Translated_282_residues
MEIECHAKVNLTLDVLGRRPDGYHEIRSVMVPVSLHDRLVLEPAEEEIVLESRPPATDRLEENLAYRAAALLREATGCSR
GAVIRLQKTIPVAAGLAGGSTDAAGVLTGLNRLWGTGLSDGELADLAIRLGSDVPFFIWSRPARVEGIGERVTPLPVAGP
LWMVVATPDVPKSTGQVYRWFDELADVGPRPDAGAMEAALARGDAAAVGRALCNVFEQVMLPRHPEIARLKEAMLAAGAL
GAVMSGAGPSVLGVVPDREAGGRLLERIRPLSRDAWVVRTLV
>Mature_282_residues
MEIECHAKVNLTLDVLGRRPDGYHEIRSVMVPVSLHDRLVLEPAEEEIVLESRPPATDRLEENLAYRAAALLREATGCSR
GAVIRLQKTIPVAAGLAGGSTDAAGVLTGLNRLWGTGLSDGELADLAIRLGSDVPFFIWSRPARVEGIGERVTPLPVAGP
LWMVVATPDVPKSTGQVYRWFDELADVGPRPDAGAMEAALARGDAAAVGRALCNVFEQVMLPRHPEIARLKEAMLAAGAL
GAVMSGAGPSVLGVVPDREAGGRLLERIRPLSRDAWVVRTLV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=179, Percent_Identity=36.8715083798883, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_SYMTH (Q67JC2)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_077072.1
- ProteinModelPortal:   Q67JC2
- SMR:   Q67JC2
- GeneID:   2979625
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3246
- NMPDR:   fig|292459.1.peg.3116
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- BioCyc:   STHE292459:STH3246-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30064; Mature: 30064

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIECHAKVNLTLDVLGRRPDGYHEIRSVMVPVSLHDRLVLEPAEEEIVLESRPPATDRL
CCEEEEEEEEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEECCCCCCEEECCCCCCHHHH
EENLAYRAAALLREATGCSRGAVIRLQKTIPVAAGLAGGSTDAAGVLTGLNRLWGTGLSD
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCC
GELADLAIRLGSDVPFFIWSRPARVEGIGERVTPLPVAGPLWMVVATPDVPKSTGQVYRW
CHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
FDELADVGPRPDAGAMEAALARGDAAAVGRALCNVFEQVMLPRHPEIARLKEAMLAAGAL
HHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GAVMSGAGPSVLGVVPDREAGGRLLERIRPLSRDAWVVRTLV
HHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure
MEIECHAKVNLTLDVLGRRPDGYHEIRSVMVPVSLHDRLVLEPAEEEIVLESRPPATDRL
CCEEEEEEEEEEEEEECCCCCCHHHHHHHCCCCCCCCEEEECCCCCCEEECCCCCCHHHH
EENLAYRAAALLREATGCSRGAVIRLQKTIPVAAGLAGGSTDAAGVLTGLNRLWGTGLSD
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCC
GELADLAIRLGSDVPFFIWSRPARVEGIGERVTPLPVAGPLWMVVATPDVPKSTGQVYRW
CHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
FDELADVGPRPDAGAMEAALARGDAAAVGRALCNVFEQVMLPRHPEIARLKEAMLAAGAL
HHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GAVMSGAGPSVLGVVPDREAGGRLLERIRPLSRDAWVVRTLV
HHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA