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The map label for this gene is purR [H]

Identifier: 51894379

GI number: 51894379

Start: 3458877

End: 3459689

Strand: Reverse

Name: purR [H]

Synonym: STH3244

Alternate gene names: 51894379

Gene position: 3459689-3458877 (Counterclockwise)

Preceding gene: 51894380

Following gene: 51894378

Centisome position: 97.02

GC content: 70.48

Gene sequence:

>813_bases
ATGAATCGGAGCCAGCGACTGGTGGCCATCATGAAACTGCTCTCCGAGCGGCCGGGAGAGCTCCTGCCGCTGAGCTTCTT
CACCGAGCGGTTCGGTGCCGCCAAGTCCACCATTTCCGAGGACCTGGCCCTGGTGAAGGAGGCCCTGGAGGCCGACGGTT
CAGGCCGCCTGCGCACGCACGCCGGGGCGGCGGGCGGGGTGCAGTACTGGCCGCTGCCCTCCCGGGAGGAGGAGCAGGAA
ACCCTGCTGGAGCTCTGCCGGCTCCTGAGCGATCCGGGGCGGATCCTGCCCGGCGGGTTCGTCTACATGACCGATCTCAT
CACCCACCCGATCTGGAGCGCCCGCATCGGGGCGATCATGGCCGCGCGGTTCATCGACGCCGAACCCGACGTCGTGCTCA
CCGTAGAGACCAAGGGGATCCCCCTGGCCCTGATGGTCGCCCGCGCCCTGGGGCTGCCCATGGTGGTCGCCCGGCGCGAG
GGCCGGGTGACGGAGGGGCCCTCCGTCACGCTCCACTACATCTCCGGATCCCGCCGCATCCACACCATGACGGTCGGCCT
CCGGGCCCTGTGGCGCGGCGCCCGGGTCCTGGTGGTCGACGACTTCATGAAGGCGGGCGCGACCGCCCGCGCCATGGTGG
ATGTGGCCGGCGAGATGGGCGCCTCCGTGGCCGGCGTGGGAGTCTTTGTCTCCACCGCGGAACCGGCCAGGAAGCAGGTG
CAGCGCTACGTGTCGCTGCTCACGCTGGAGCAGGTGGACGAGGTGGCCCGCACGGTCCGGGTGGTTCCGGCCGACCGACT
GACGAAGGAGTGA

Upstream 100 bases:

>100_bases
GGGCATCGACGTGGACAAGCCGAGCGACCTGGGCCTGGTGGAGTCCCTTCTGAAATAGCGGGCCGTCCGGGGCGGACAGG
GGGTGGCAGCGATCGATCGG

Downstream 100 bases:

>100_bases
ACCGTGGGTGACGGTAACCTTTGCGGACATCGAGAAGGCGCGCATTCTGCTGAGCGGACACATCAAGCGGACACCCATGC
TTCAGAGCCCTGGCATCACC

Product: pur operon repressor

Products: L-Glutamate; Diphosphate; 5-Phospho-beta-D-ribosylamine [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNRSQRLVAIMKLLSERPGELLPLSFFTERFGAAKSTISEDLALVKEALEADGSGRLRTHAGAAGGVQYWPLPSREEEQE
TLLELCRLLSDPGRILPGGFVYMTDLITHPIWSARIGAIMAARFIDAEPDVVLTVETKGIPLALMVARALGLPMVVARRE
GRVTEGPSVTLHYISGSRRIHTMTVGLRALWRGARVLVVDDFMKAGATARAMVDVAGEMGASVAGVGVFVSTAEPARKQV
QRYVSLLTLEQVDEVARTVRVVPADRLTKE

Sequences:

>Translated_270_residues
MNRSQRLVAIMKLLSERPGELLPLSFFTERFGAAKSTISEDLALVKEALEADGSGRLRTHAGAAGGVQYWPLPSREEEQE
TLLELCRLLSDPGRILPGGFVYMTDLITHPIWSARIGAIMAARFIDAEPDVVLTVETKGIPLALMVARALGLPMVVARRE
GRVTEGPSVTLHYISGSRRIHTMTVGLRALWRGARVLVVDDFMKAGATARAMVDVAGEMGASVAGVGVFVSTAEPARKQV
QRYVSLLTLEQVDEVARTVRVVPADRLTKE
>Mature_270_residues
MNRSQRLVAIMKLLSERPGELLPLSFFTERFGAAKSTISEDLALVKEALEADGSGRLRTHAGAAGGVQYWPLPSREEEQE
TLLELCRLLSDPGRILPGGFVYMTDLITHPIWSARIGAIMAARFIDAEPDVVLTVETKGIPLALMVARALGLPMVVARRE
GRVTEGPSVTLHYISGSRRIHTMTVGLRALWRGARVLVVDDFMKAGATARAMVDVAGEMGASVAGVGVFVSTAEPARKQV
QRYVSLLTLEQVDEVARTVRVVPADRLTKE

Specific function: Controls the transcription of the pur operon for purine biosynthetic genes, binds to the control region of the operon. DNA binding is inhibited by 5-phosphoribosyl 1-pyrophosphate [H]

COG id: COG0503

COG function: function code F; Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000836
- InterPro:   IPR015265
- InterPro:   IPR010078
- InterPro:   IPR011991
- ProDom:   PD029816 [H]

Pfam domain/function: PF00156 Pribosyltran; PF09182 PuR_N [H]

EC number: 2.4.2.14 [C]

Molecular weight: Translated: 29344; Mature: 29344

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSQRLVAIMKLLSERPGELLPLSFFTERFGAAKSTISEDLALVKEALEADGSGRLRTH
CCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
AGAAGGVQYWPLPSREEEQETLLELCRLLSDPGRILPGGFVYMTDLITHPIWSARIGAIM
CCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
AARFIDAEPDVVLTVETKGIPLALMVARALGLPMVVARREGRVTEGPSVTLHYISGSRRI
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCEE
HTMTVGLRALWRGARVLVVDDFMKAGATARAMVDVAGEMGASVAGVGVFVSTAEPARKQV
EEHHHHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHH
QRYVSLLTLEQVDEVARTVRVVPADRLTKE
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MNRSQRLVAIMKLLSERPGELLPLSFFTERFGAAKSTISEDLALVKEALEADGSGRLRTH
CCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
AGAAGGVQYWPLPSREEEQETLLELCRLLSDPGRILPGGFVYMTDLITHPIWSARIGAIM
CCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
AARFIDAEPDVVLTVETKGIPLALMVARALGLPMVVARREGRVTEGPSVTLHYISGSRRI
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCEE
HTMTVGLRALWRGARVLVVDDFMKAGATARAMVDVAGEMGASVAGVGVFVSTAEPARKQV
EEHHHHHHHHHCCCEEEEEEHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHH
QRYVSLLTLEQVDEVARTVRVVPADRLTKE
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Mg-phosphoribosyldiphosphate Mg-phosphoribosyldiphosphate, [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.067 {5-phospho-alpha-D-ribose} 1.7 {glutamine}} 8.8 {NH3}} [C]

Substrates: L-Glutamine; H2O; 5-Phospho-alpha-D-ribose 1-diphosphate [C]

Specific reaction: L-Glutamine + H2O + 5-Phospho-alpha-D-ribose 1-diphosphate --> L-Glutamate + Diphosphate + 5-Phospho-beta-D-ribosylamine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2-Mercaptoethanol; 6-Diazo-5-oxo-L-norleucine analogue [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 7638212 [H]