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The map label for this gene is ltaA [H]

Identifier: 51893541

GI number: 51893541

Start: 2587613

End: 2588653

Strand: Reverse

Name: ltaA [H]

Synonym: STH2403

Alternate gene names: 51893541

Gene position: 2588653-2587613 (Counterclockwise)

Preceding gene: 51893542

Following gene: 51893540

Centisome position: 72.59

GC content: 72.24

Gene sequence:

>1041_bases
ATGCGGGCAATCGACCTGCGAAGCGACACCGTAACCAAGCCCACGCCGGAGATGCGGCGGGCGATGTACGAGGCGGAAGT
CGGCGACGATGTGTACGGCGAGGACCCGACGATCAACCGGCTGGAGGAGCGGGCCGCGGAGCTCCTGGGGAAGGAGGCGG
CGCTGTTCGTGCCCAGCGGCACGATGGGCAACCAGGTGGCGGTGCTCACCCATACGCGCCGGGGCCAGGAGGTCATCGTC
GAGGCCGAGAGCCACGTCTACCTGTACGAGGTCGGCGGGATCGCCGCCCTGTCGAGCTGCCAGGTGAAGACCATCCCCGG
CGTGCGGGGCGCGATGGACCCGGCCGCGGTGGAGGCGGCCATCCGGGCGGACAACGTCCACTTCCCCCGCACCGGGCTGG
TCTGCGTCGAGAACACCCACAACCGCTCGGGCGGTTGTGTGCTTCCCCCCGAGAACGTGGCGGCCGTCGCCGCGGTCGCC
CACCGGCACGGCATCCCGGTGCACATGGACGGCGCCCGCATCTTCAACGCGGCCGTGGCCCTGGGGCGGCCCGCGGCCGA
GCTGGTCGCCCCGGTGGATTCGGTGATGTTCTGTCTCTCCAAGGGGCTCGCGGCCCCGGTGGGCTCGCTGCTCGTGGGCA
GCAGGGAGTTCATCGCCGAGGCCCGCCGCTACCGGAAGCTCCTGGGCGGCGGCATGCGCCAGGCCGGTGTCCTGGCGGCG
GCCGGGCTGGTCGCGCTGGAGATGATGATCGACCGGCTGGCCGACGACCACGCCAACGCCCGCCGGCTGGCCGAGGGGAT
CGCCGGGATCCCCGGCCTGCGGGTGGACCTGGAGACGGTGCAGACCAACATGGTCGTGGTGGAGATCGTCGACGAACGCT
GGACCGCGGGCGCCTTCGTGCAGGCGCTGCGGACGCAGGGCGTGCTGTGCAACGACACCGGGCCGCGCAGCGTCCGGCTG
GTCACCCACAAGGACGTGACTGCGGCTGACGTGGAGCTGGCCCTCGACGCCATCGCCCGCACGGTGAAGGCGGGGCCCTA
G

Upstream 100 bases:

>100_bases
CCGCCAAGATCGACCGGGCTCTGGCCCAGGCCTAGATGACGGAGCGGGCGACCCCTGGCGGGTCGCTCGTTTTCACACTT
GAAGGGATGGGGCGAAGAAC

Downstream 100 bases:

>100_bases
CCATGTCCGACACGCAGCAGCTGACGCTGATCGCCACCGCCGCCATGGGGCTGGAGGCGGTGGTGGCGCGCGAGCTGAAG
GGCCTGGGCTACGACCAGCA

Product: threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRAIDLRSDTVTKPTPEMRRAMYEAEVGDDVYGEDPTINRLEERAAELLGKEAALFVPSGTMGNQVAVLTHTRRGQEVIV
EAESHVYLYEVGGIAALSSCQVKTIPGVRGAMDPAAVEAAIRADNVHFPRTGLVCVENTHNRSGGCVLPPENVAAVAAVA
HRHGIPVHMDGARIFNAAVALGRPAAELVAPVDSVMFCLSKGLAAPVGSLLVGSREFIAEARRYRKLLGGGMRQAGVLAA
AGLVALEMMIDRLADDHANARRLAEGIAGIPGLRVDLETVQTNMVVVEIVDERWTAGAFVQALRTQGVLCNDTGPRSVRL
VTHKDVTAADVELALDAIARTVKAGP

Sequences:

>Translated_346_residues
MRAIDLRSDTVTKPTPEMRRAMYEAEVGDDVYGEDPTINRLEERAAELLGKEAALFVPSGTMGNQVAVLTHTRRGQEVIV
EAESHVYLYEVGGIAALSSCQVKTIPGVRGAMDPAAVEAAIRADNVHFPRTGLVCVENTHNRSGGCVLPPENVAAVAAVA
HRHGIPVHMDGARIFNAAVALGRPAAELVAPVDSVMFCLSKGLAAPVGSLLVGSREFIAEARRYRKLLGGGMRQAGVLAA
AGLVALEMMIDRLADDHANARRLAEGIAGIPGLRVDLETVQTNMVVVEIVDERWTAGAFVQALRTQGVLCNDTGPRSVRL
VTHKDVTAADVELALDAIARTVKAGP
>Mature_346_residues
MRAIDLRSDTVTKPTPEMRRAMYEAEVGDDVYGEDPTINRLEERAAELLGKEAALFVPSGTMGNQVAVLTHTRRGQEVIV
EAESHVYLYEVGGIAALSSCQVKTIPGVRGAMDPAAVEAAIRADNVHFPRTGLVCVENTHNRSGGCVLPPENVAAVAAVA
HRHGIPVHMDGARIFNAAVALGRPAAELVAPVDSVMFCLSKGLAAPVGSLLVGSREFIAEARRYRKLLGGGMRQAGVLAA
AGLVALEMMIDRLADDHANARRLAEGIAGIPGLRVDLETVQTNMVVVEIVDERWTAGAFVQALRTQGVLCNDTGPRSVRL
VTHKDVTAADVELALDAIARTVKAGP

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=327, Percent_Identity=48.3180428134557, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI193206524, Length=351, Percent_Identity=44.4444444444444, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI193206522, Length=351, Percent_Identity=44.4444444444444, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI193206520, Length=351, Percent_Identity=44.4444444444444, Blast_Score=261, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6320789, Length=298, Percent_Identity=39.261744966443, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21355439, Length=307, Percent_Identity=49.8371335504886, Blast_Score=278, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 36671; Mature: 36671

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAIDLRSDTVTKPTPEMRRAMYEAEVGDDVYGEDPTINRLEERAAELLGKEAALFVPSG
CCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCC
TMGNQVAVLTHTRRGQEVIVEAESHVYLYEVGGIAALSSCQVKTIPGVRGAMDPAAVEAA
CCCCEEEEEEECCCCCEEEEEECCCEEEEECCCEEECCCCCEEECCCCCCCCCHHHHHHH
IRADNVHFPRTGLVCVENTHNRSGGCVLPPENVAAVAAVAHRHGIPVHMDGARIFNAAVA
HHCCCCCCCCCCEEEEECCCCCCCCEECCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHH
LGRPAAELVAPVDSVMFCLSKGLAAPVGSLLVGSREFIAEARRYRKLLGGGMRQAGVLAA
CCCCHHHHHHHHHHHHHHHHCCHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHH
AGLVALEMMIDRLADDHANARRLAEGIAGIPGLRVDLETVQTNMVVVEIVDERWTAGAFV
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEHHHHHCCEEEEEEECCCCCHHHHH
QALRTQGVLCNDTGPRSVRLVTHKDVTAADVELALDAIARTVKAGP
HHHHHCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRAIDLRSDTVTKPTPEMRRAMYEAEVGDDVYGEDPTINRLEERAAELLGKEAALFVPSG
CCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCC
TMGNQVAVLTHTRRGQEVIVEAESHVYLYEVGGIAALSSCQVKTIPGVRGAMDPAAVEAA
CCCCEEEEEEECCCCCEEEEEECCCEEEEECCCEEECCCCCEEECCCCCCCCCHHHHHHH
IRADNVHFPRTGLVCVENTHNRSGGCVLPPENVAAVAAVAHRHGIPVHMDGARIFNAAVA
HHCCCCCCCCCCEEEEECCCCCCCCEECCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHH
LGRPAAELVAPVDSVMFCLSKGLAAPVGSLLVGSREFIAEARRYRKLLGGGMRQAGVLAA
CCCCHHHHHHHHHHHHHHHHCCHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHH
AGLVALEMMIDRLADDHANARRLAEGIAGIPGLRVDLETVQTNMVVVEIVDERWTAGAFV
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEHHHHHCCEEEEEEECCCCCHHHHH
QALRTQGVLCNDTGPRSVRLVTHKDVTAADVELALDAIARTVKAGP
HHHHHCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]