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The map label for this gene is gcvH

Identifier: 51893059

GI number: 51893059

Start: 2065109

End: 2065495

Strand: Reverse

Name: gcvH

Synonym: STH1921

Alternate gene names: 51893059

Gene position: 2065495-2065109 (Counterclockwise)

Preceding gene: 51893060

Following gene: 51893058

Centisome position: 57.92

GC content: 61.5

Gene sequence:

>387_bases
ATGGCGAACGTACCGGCGGATCTCAAGTACTCGAAGGAGCACGAGTGGATCAGGGTCGAAGGCAACATCGGCATCGTGGG
CATCACCTGGTTCGCCCAGGATCAGTTGGGCGACGTGGTCTTCGTCGAGCTGCCTGAGGTGGGGCGTGAACTGAAGCAGA
ACGAGCAGTTCGGCGTGGTGGAGTCGGTCAAGACCGTTTCGGACCTGTACTGCCCGGCCTCCGGCAAGGTGGTGGAGGTC
AACACGAAGCTGGAGAGCTCGCCGGAGCTGATCAACCAGGACCCCTACGGCGAGGGCTGGATTCTGAAGCTGGAGCTCTC
GAACCCGGCCGAACTGGACAACCTGCTGGATGCGGCGGCTTACGACGCGTTTACCAAGGAGGGCTAA

Upstream 100 bases:

>100_bases
CGGGCGCTGAAGGCGCGGGTCGTGGAGACCCCCTTCTACCGGTCGCCGCACAGACGATAACCGGCACGGTTGAGAATTAC
GCACAGGGAGGACGCGGAAC

Downstream 100 bases:

>100_bases
ATGCGATACGTCCCGATTACGCAGGCCGACAAGGCGGCCATGCTGCGGGAGATCGGCGTCAGTTCCACCGAGGAGCTCTT
TGCTGCCAACATCCCGGCGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MANVPADLKYSKEHEWIRVEGNIGIVGITWFAQDQLGDVVFVELPEVGRELKQNEQFGVVESVKTVSDLYCPASGKVVEV
NTKLESSPELINQDPYGEGWILKLELSNPAELDNLLDAAAYDAFTKEG

Sequences:

>Translated_128_residues
MANVPADLKYSKEHEWIRVEGNIGIVGITWFAQDQLGDVVFVELPEVGRELKQNEQFGVVESVKTVSDLYCPASGKVVEV
NTKLESSPELINQDPYGEGWILKLELSNPAELDNLLDAAAYDAFTKEG
>Mature_127_residues
ANVPADLKYSKEHEWIRVEGNIGIVGITWFAQDQLGDVVFVELPEVGRELKQNEQFGVVESVKTVSDLYCPASGKVVEVN
TKLESSPELINQDPYGEGWILKLELSNPAELDNLLDAAAYDAFTKEG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=49.1525423728814, Blast_Score=131, Evalue=2e-31,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=48.3050847457627, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1789271, Length=128, Percent_Identity=53.90625, Blast_Score=142, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=50.8474576271186, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=49.1525423728814, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=44.2622950819672, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17865652, Length=117, Percent_Identity=48.7179487179487, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_SYMTH (Q67N37)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_075750.1
- HSSP:   Q9WY55
- ProteinModelPortal:   Q67N37
- SMR:   Q67N37
- GeneID:   2980569
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH1921
- NMPDR:   fig|292459.1.peg.1854
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- BioCyc:   STHE292459:STH1921-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14169; Mature: 14038

Theoretical pI: Translated: 4.01; Mature: 4.01

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVPADLKYSKEHEWIRVEGNIGIVGITWFAQDQLGDVVFVELPEVGRELKQNEQFGVV
CCCCCCCCEECCCCCEEEEECCEEEEEEEEEECCCCCCEEEEECCHHHHHHHCCCCCCHH
ESVKTVSDLYCPASGKVVEVNTKLESSPELINQDPYGEGWILKLELSNPAELDNLLDAAA
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
YDAFTKEG
HHHHCCCC
>Mature Secondary Structure 
ANVPADLKYSKEHEWIRVEGNIGIVGITWFAQDQLGDVVFVELPEVGRELKQNEQFGVV
CCCCCCCEECCCCCEEEEECCEEEEEEEEEECCCCCCEEEEECCHHHHHHHCCCCCCHH
ESVKTVSDLYCPASGKVVEVNTKLESSPELINQDPYGEGWILKLELSNPAELDNLLDAAA
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
YDAFTKEG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA