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The map label for this gene is metK

Identifier: 50842673

GI number: 50842673

Start: 1298566

End: 1299759

Strand: Direct

Name: metK

Synonym: PPA1193

Alternate gene names: 50842673

Gene position: 1298566-1299759 (Clockwise)

Preceding gene: 50842672

Following gene: 50842674

Centisome position: 50.72

GC content: 59.72

Gene sequence:

>1194_bases
ATGCCTACTCGTCTGTTCACATCGGAATCAGTAACGGAAGGCCACCCGGACAAGATTGCCGACGCCATTTCCGACGCTGT
CCTTGATGCTATGTTGACCGAGGACTCCCATTCTCACGCTGCCGTGGAAACCGTCGTGACGACCGGGCAGGTAATGGTGT
GTGGCGAGGTCACCACCGAGGCCTATGTCGACATCGCCGACATCGCCCGCTCCCGCATTCTTGACATCGGTTACGACTCG
TCTTCCAAGGGTTTTGACGGAGCTTCGTGTGGCGTTTCGGTCGCAATCGATGCTCAGAGCCCTGACATTGCCCAAGGGGT
GACCGCGGCCTACGAAACTCGTCATGGTTCCACCGATCTTATTGACTCCCAAGGTGCTGGAGACCAGGGCTTGATGTTCG
GATATGCGTGCACCGAGACCCCTTCTCTCATGCCGTTACCTATTGACATGGCCCATGCGCTGTCCTTGCAGCTGACAAAG
GTCCGCAAAGAAGGTGCACTGGACTATCTGTGCCCTGACGGCAAGACCCAGGTGACAATTCGTTACAACGAGGACGATAA
ACCCGTGGCGGTGGACACCGTCATCGTGTCGAGCCAGCATCGTGCGGGAATCGACCTGGACGCGACGATGACCCCCGATC
TCCGTCGCCTTGTAATTGACCCGGTGTTGGAGCGTTACGACCTCGATCATAAGGACATGCGGGTGCTCGTTAACCCGACC
GGCAAGTTCGTTATCGGCGGCCCTATGGGTGACGCCGGGCTGACCGGCCGCAAGATCATCGTCGACACTTATGGCGGTAT
GGCACGTCATGGGGGAGGTGCCTTCTCCGGCAAAGATCCGTCCAAGGTCGACCGTTCCGGTGCCTATGCGATGCGCTGGG
TAGCCAAGAACGTCGTCGCAGCTGGACTAGCCGATCGTTGCGAGTGCCAAGTGGCTTACGCCATCGGTGCTGCCCGCCCG
GTGGGATTCCGTATTGACACCTTTGGTACCAACCACGTGCCCGAGACTGTCATCGAGCGCGCTGTCGGTGAGGTCTTTGA
TCTACGACCCGGTGCGATCATCGCGGACCTCGACCTGCTGCGACCGATCTATTCCCAGCTCGTTGCTGGTGGCCACTTCG
GTCGTGAGCTTCCCGGGGTCACCTGGGAGCTGACGGACCGCGCCGAGGCCTTGGCAGCAACCGCCCGTCTCTGA

Upstream 100 bases:

>100_bases
GAAGTCGCAGGCGGCTGTCGCCATCCTTGACGAGGTCCGCGCGCTGCTGGCTACTCAGTAACACGATGCGCGGCTTGTGG
CCGCGCGGACAGGAAACGTC

Downstream 100 bases:

>100_bases
GAGAACACACCGCGACGCCGTCGGTGGTGGGCCGTAACATGTGCCCCATGACCGCACCGCTATTGGAGACGACGTCATGG
ATCGCCCATGTCGCCGTCTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MPTRLFTSESVTEGHPDKIADAISDAVLDAMLTEDSHSHAAVETVVTTGQVMVCGEVTTEAYVDIADIARSRILDIGYDS
SSKGFDGASCGVSVAIDAQSPDIAQGVTAAYETRHGSTDLIDSQGAGDQGLMFGYACTETPSLMPLPIDMAHALSLQLTK
VRKEGALDYLCPDGKTQVTIRYNEDDKPVAVDTVIVSSQHRAGIDLDATMTPDLRRLVIDPVLERYDLDHKDMRVLVNPT
GKFVIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYAMRWVAKNVVAAGLADRCECQVAYAIGAARP
VGFRIDTFGTNHVPETVIERAVGEVFDLRPGAIIADLDLLRPIYSQLVAGGHFGRELPGVTWELTDRAEALAATARL

Sequences:

>Translated_397_residues
MPTRLFTSESVTEGHPDKIADAISDAVLDAMLTEDSHSHAAVETVVTTGQVMVCGEVTTEAYVDIADIARSRILDIGYDS
SSKGFDGASCGVSVAIDAQSPDIAQGVTAAYETRHGSTDLIDSQGAGDQGLMFGYACTETPSLMPLPIDMAHALSLQLTK
VRKEGALDYLCPDGKTQVTIRYNEDDKPVAVDTVIVSSQHRAGIDLDATMTPDLRRLVIDPVLERYDLDHKDMRVLVNPT
GKFVIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYAMRWVAKNVVAAGLADRCECQVAYAIGAARP
VGFRIDTFGTNHVPETVIERAVGEVFDLRPGAIIADLDLLRPIYSQLVAGGHFGRELPGVTWELTDRAEALAATARL
>Mature_396_residues
PTRLFTSESVTEGHPDKIADAISDAVLDAMLTEDSHSHAAVETVVTTGQVMVCGEVTTEAYVDIADIARSRILDIGYDSS
SKGFDGASCGVSVAIDAQSPDIAQGVTAAYETRHGSTDLIDSQGAGDQGLMFGYACTETPSLMPLPIDMAHALSLQLTKV
RKEGALDYLCPDGKTQVTIRYNEDDKPVAVDTVIVSSQHRAGIDLDATMTPDLRRLVIDPVLERYDLDHKDMRVLVNPTG
KFVIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYAMRWVAKNVVAAGLADRCECQVAYAIGAARPV
GFRIDTFGTNHVPETVIERAVGEVFDLRPGAIIADLDLLRPIYSQLVAGGHFGRELPGVTWELTDRAEALAATARL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=384, Percent_Identity=52.6041666666667, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI5174529, Length=388, Percent_Identity=51.5463917525773, Blast_Score=347, Evalue=9e-96,
Organism=Escherichia coli, GI1789311, Length=397, Percent_Identity=56.9269521410579, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538494, Length=387, Percent_Identity=52.4547803617571, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145633, Length=386, Percent_Identity=52.3316062176166, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17538498, Length=392, Percent_Identity=52.0408163265306, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=49.4818652849741, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=50.2941176470588, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI32565911, Length=340, Percent_Identity=50.2941176470588, Blast_Score=300, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI32565905, Length=387, Percent_Identity=47.0284237726098, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI32565903, Length=315, Percent_Identity=50.1587301587302, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=59.0361445783133, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323209, Length=388, Percent_Identity=52.319587628866, Blast_Score=361, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=49.8727735368957, Blast_Score=354, Evalue=2e-98,
Organism=Drosophila melanogaster, GI45552159, Length=384, Percent_Identity=49.21875, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580529, Length=384, Percent_Identity=49.21875, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580537, Length=384, Percent_Identity=49.21875, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580533, Length=384, Percent_Identity=49.21875, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580535, Length=384, Percent_Identity=49.21875, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580531, Length=384, Percent_Identity=49.21875, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580527, Length=384, Percent_Identity=49.21875, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24580525, Length=384, Percent_Identity=49.21875, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24580541, Length=370, Percent_Identity=47.8378378378378, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=50.3937007874016, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PROAC (Q6A8H3)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055900.1
- ProteinModelPortal:   Q6A8H3
- SMR:   Q6A8H3
- GeneID:   2933073
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1193
- NMPDR:   fig|267747.1.peg.1177
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   PACN267747:PPA1193-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42251; Mature: 42120

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTRLFTSESVTEGHPDKIADAISDAVLDAMLTEDSHSHAAVETVVTTGQVMVCGEVTTE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEECCCEEEEECCCHH
AYVDIADIARSRILDIGYDSSSKGFDGASCGVSVAIDAQSPDIAQGVTAAYETRHGSTDL
HHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCC
IDSQGAGDQGLMFGYACTETPSLMPLPIDMAHALSLQLTKVRKEGALDYLCPDGKTQVTI
CCCCCCCCCCEEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
RYNEDDKPVAVDTVIVSSQHRAGIDLDATMTPDLRRLVIDPVLERYDLDHKDMRVLVNPT
EECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCC
GKFVIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYAMRWVAKNVVA
CCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
AGLADRCECQVAYAIGAARPVGFRIDTFGTNHVPETVIERAVGEVFDLRPGAIIADLDLL
HCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECHHHH
RPIYSQLVAGGHFGRELPGVTWELTDRAEALAATARL
HHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHCCC
>Mature Secondary Structure 
PTRLFTSESVTEGHPDKIADAISDAVLDAMLTEDSHSHAAVETVVTTGQVMVCGEVTTE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEECCCEEEEECCCHH
AYVDIADIARSRILDIGYDSSSKGFDGASCGVSVAIDAQSPDIAQGVTAAYETRHGSTDL
HHHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCC
IDSQGAGDQGLMFGYACTETPSLMPLPIDMAHALSLQLTKVRKEGALDYLCPDGKTQVTI
CCCCCCCCCCEEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
RYNEDDKPVAVDTVIVSSQHRAGIDLDATMTPDLRRLVIDPVLERYDLDHKDMRVLVNPT
EECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCC
GKFVIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSGAYAMRWVAKNVVA
CCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
AGLADRCECQVAYAIGAARPVGFRIDTFGTNHVPETVIERAVGEVFDLRPGAIIADLDLL
HCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECHHHH
RPIYSQLVAGGHFGRELPGVTWELTDRAEALAATARL
HHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA