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The map label for this gene is priA [H]

Identifier: 50842674

GI number: 50842674

Start: 1299798

End: 1301816

Strand: Direct

Name: priA [H]

Synonym: PPA1194

Alternate gene names: 50842674

Gene position: 1299798-1301816 (Clockwise)

Preceding gene: 50842673

Following gene: 50842675

Centisome position: 50.77

GC content: 61.02

Gene sequence:

>2019_bases
ATGTGCCCCATGACCGCACCGCTATTGGAGACGACGTCATGGATCGCCCATGTCGCCGTCTCTTCCGGTTTGGCCCACCT
TGACCGTCCTTTCGACTATTTGATTCCTGACGACCTCGTCGATCGAGTCCAGGTTGGGGTACGAGTGCGGGTCAAACTCG
CCGGTCGGCTGTGTGACGGCGTCGTGGTGGCCGTCGATCATGAGCAACAGCCTGGGGTCACGTTGGCCCCGGTTGAAAAA
CTGGTGTCCGACCAGGTAGTTGCTACACCAGATCAGCTCAACTTTGCCCGTCATGTGGCTGACCACTGGGCAGGCACCCT
GGACGAGGTGCTCAGATGGGCTATCCCTACCCGGCACGCCACGACAGAGACAGCCAATCCTCCACCGTGGCCAACGCCGA
TGACGACGATGCCGTCTGGAACCTTGACGGCCTTAGACCGGGGGCGCCGCTTCCTTGCACTGCTTAAGGCCGGAGAACGC
CCTCGCGCCCACTGGCGGGTCAGTCCAGTTTGTGGTCCGGGGGATGATGATCACCTCGGAGATTGGGGCAACGGTTTACT
GCAAGCAGTCTGCGCCACGTTGACGTCAGGACGCAGTGCGGTGGTGTGTTGCCCAACGGTTGACGACGTCATCACCCTAC
ATGAGAGATTCCAAGCTGTTTTAGGAGCTGGAACCGTCGCGGTGCTCCACGCTGATCAACCAGCGTCAACGCGCTGGCGC
CACTACCTGGCAGTCATTCGTGGCACAGCGAAAGTTGTGGTAGGAACTCGCTCGGCCGTCATGGCGCCAATGACGGACCT
GGGATTGATCGCTGTCTGGGATGAAGGATCCGACCTCCATGACGAACCACGAGCTCCATACCCGAATACCCGCGATGTCG
CTGCCCTGCGAGCCCAGATTGACCACTGTGCGTTGTTATTGGCGAGCTATTCCTGTTCGGTGCGCAGCGCCTCGTGGCTA
GGGTCAGGTTGGCTCGTCGGAATCGGCGCGGACCGTCCAACGATGAGAGCAGTCTGTGCACCGGTTCGCATTCCCGGTGA
CTCCGATATCGCTATGGAACGTGACCCGTTGGCGCGATCAGTTCGGCTTCCCGATCTTGCTATGACGACCATCCGTGATG
GATTGTTGCATGGCCCCGTTCTGGTTTCCGTTCCGCGAGTTGGCGATCTCGTCACCCCATCGTGTGCACGCTGCCGAACT
CCAGTGCGATGTTCTTCGTGCGGTGGACCGGTTACCGGGCGGCGTACTGAATCTGGAGTCAACCTCCATTGCACCTGGTG
TGGCAGTCACTTACCACCGTGGCGCTGTCCCGAATGCGGCTCCACTGAAGTGAGGTCAGGCATTGTGGGAGCGACGACGA
CGGCCGCAGAATTGGGCCGGGCATTTCCAGATGTTCCCGTGGTTGATTCGTCCGGCAATCACGTTCGGGAGAGGGTCGAT
TCAACTCCCCGATTAATCGTTGCCACCCCAGGGGCCGAACCCACACCAGAATCGGGCTATGCCTGTGCTGTCATCCTGGA
TGCGGTGTCCTCCTTGTCGAGGCCTGGCCTGGCGGCGGTGGAGCAGACCGTCGATCGGTGGATGGCAATCCTGGCCTTGG
TCCGATCAGTGCGGGATGGGGGCCGGGCAGTTATCGTCGGGCCTTCGGAGGACGCCGCCTTGCAGGCCATGGTTCGAAAT
GATCCAGTCGGGTGGGCGACACGTGAACTCGCCGATCGTCGGGAGGCACATTTCCCGCCCGCGGTGCCGTGCGGAATTGT
CGACGGTGACCCGAAAGCGGTGGCTACAGCGGCACAGCGACTACGCGAGTGGTTCGGAACCGACCTTGAGATGCTTGGCC
CAGCTCCACAACCACGCCGTGCCAGCGAATCGGAACGGGATCGAATTATCGTGCGTCCTCGTGGCACGATGCCTCTCGCC
GAGCTTTCCCAAGGTCTATTTCGGCTACGTTCCAAGCACACTATGAGCCGCGAGCCAGGAAGCTTACGCGTGGTCATCGA
CCCGGCCAACCTGTTGTGA

Upstream 100 bases:

>100_bases
CCGGGGTCACCTGGGAGCTGACGGACCGCGCCGAGGCCTTGGCAGCAACCGCCCGTCTCTGAGAGAACACACCGCGACGC
CGTCGGTGGTGGGCCGTAAC

Downstream 100 bases:

>100_bases
GGTCGGTAGGCTTGCGGTGTGAGACTTCTTTTTGCTGGTACCCCGGACGTGGCCGTCCCAACGCTTACCGCCTTGGTAGC
CGATCCCCGTCACGAGGTAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MCPMTAPLLETTSWIAHVAVSSGLAHLDRPFDYLIPDDLVDRVQVGVRVRVKLAGRLCDGVVVAVDHEQQPGVTLAPVEK
LVSDQVVATPDQLNFARHVADHWAGTLDEVLRWAIPTRHATTETANPPPWPTPMTTMPSGTLTALDRGRRFLALLKAGER
PRAHWRVSPVCGPGDDDHLGDWGNGLLQAVCATLTSGRSAVVCCPTVDDVITLHERFQAVLGAGTVAVLHADQPASTRWR
HYLAVIRGTAKVVVGTRSAVMAPMTDLGLIAVWDEGSDLHDEPRAPYPNTRDVAALRAQIDHCALLLASYSCSVRSASWL
GSGWLVGIGADRPTMRAVCAPVRIPGDSDIAMERDPLARSVRLPDLAMTTIRDGLLHGPVLVSVPRVGDLVTPSCARCRT
PVRCSSCGGPVTGRRTESGVNLHCTWCGSHLPPWRCPECGSTEVRSGIVGATTTAAELGRAFPDVPVVDSSGNHVRERVD
STPRLIVATPGAEPTPESGYACAVILDAVSSLSRPGLAAVEQTVDRWMAILALVRSVRDGGRAVIVGPSEDAALQAMVRN
DPVGWATRELADRREAHFPPAVPCGIVDGDPKAVATAAQRLREWFGTDLEMLGPAPQPRRASESERDRIIVRPRGTMPLA
ELSQGLFRLRSKHTMSREPGSLRVVIDPANLL

Sequences:

>Translated_672_residues
MCPMTAPLLETTSWIAHVAVSSGLAHLDRPFDYLIPDDLVDRVQVGVRVRVKLAGRLCDGVVVAVDHEQQPGVTLAPVEK
LVSDQVVATPDQLNFARHVADHWAGTLDEVLRWAIPTRHATTETANPPPWPTPMTTMPSGTLTALDRGRRFLALLKAGER
PRAHWRVSPVCGPGDDDHLGDWGNGLLQAVCATLTSGRSAVVCCPTVDDVITLHERFQAVLGAGTVAVLHADQPASTRWR
HYLAVIRGTAKVVVGTRSAVMAPMTDLGLIAVWDEGSDLHDEPRAPYPNTRDVAALRAQIDHCALLLASYSCSVRSASWL
GSGWLVGIGADRPTMRAVCAPVRIPGDSDIAMERDPLARSVRLPDLAMTTIRDGLLHGPVLVSVPRVGDLVTPSCARCRT
PVRCSSCGGPVTGRRTESGVNLHCTWCGSHLPPWRCPECGSTEVRSGIVGATTTAAELGRAFPDVPVVDSSGNHVRERVD
STPRLIVATPGAEPTPESGYACAVILDAVSSLSRPGLAAVEQTVDRWMAILALVRSVRDGGRAVIVGPSEDAALQAMVRN
DPVGWATRELADRREAHFPPAVPCGIVDGDPKAVATAAQRLREWFGTDLEMLGPAPQPRRASESERDRIIVRPRGTMPLA
ELSQGLFRLRSKHTMSREPGSLRVVIDPANLL
>Mature_672_residues
MCPMTAPLLETTSWIAHVAVSSGLAHLDRPFDYLIPDDLVDRVQVGVRVRVKLAGRLCDGVVVAVDHEQQPGVTLAPVEK
LVSDQVVATPDQLNFARHVADHWAGTLDEVLRWAIPTRHATTETANPPPWPTPMTTMPSGTLTALDRGRRFLALLKAGER
PRAHWRVSPVCGPGDDDHLGDWGNGLLQAVCATLTSGRSAVVCCPTVDDVITLHERFQAVLGAGTVAVLHADQPASTRWR
HYLAVIRGTAKVVVGTRSAVMAPMTDLGLIAVWDEGSDLHDEPRAPYPNTRDVAALRAQIDHCALLLASYSCSVRSASWL
GSGWLVGIGADRPTMRAVCAPVRIPGDSDIAMERDPLARSVRLPDLAMTTIRDGLLHGPVLVSVPRVGDLVTPSCARCRT
PVRCSSCGGPVTGRRTESGVNLHCTWCGSHLPPWRCPECGSTEVRSGIVGATTTAAELGRAFPDVPVVDSSGNHVRERVD
STPRLIVATPGAEPTPESGYACAVILDAVSSLSRPGLAAVEQTVDRWMAILALVRSVRDGGRAVIVGPSEDAALQAMVRN
DPVGWATRELADRREAHFPPAVPCGIVDGDPKAVATAAQRLREWFGTDLEMLGPAPQPRRASESERDRIIVRPRGTMPLA
ELSQGLFRLRSKHTMSREPGSLRVVIDPANLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=488, Percent_Identity=23.155737704918, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 72118; Mature: 72118

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCPMTAPLLETTSWIAHVAVSSGLAHLDRPFDYLIPDDLVDRVQVGVRVRVKLAGRLCDG
CCCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHCCEEEEEEEECCCCCC
VVVAVDHEQQPGVTLAPVEKLVSDQVVATPDQLNFARHVADHWAGTLDEVLRWAIPTRHA
EEEEEECCCCCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTETANPPPWPTPMTTMPSGTLTALDRGRRFLALLKAGERPRAHWRVSPVCGPGDDDHLG
CCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCC
DWGNGLLQAVCATLTSGRSAVVCCPTVDDVITLHERFQAVLGAGTVAVLHADQPASTRWR
CHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
HYLAVIRGTAKVVVGTRSAVMAPMTDLGLIAVWDEGSDLHDEPRAPYPNTRDVAALRAQI
HHHHHHHCCEEEEEECCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
DHCALLLASYSCSVRSASWLGSGWLVGIGADRPTMRAVCAPVRIPGDSDIAMERDPLARS
HHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCEECCCCCCCCCCCCCHHHH
VRLPDLAMTTIRDGLLHGPVLVSVPRVGDLVTPSCARCRTPVRCSSCGGPVTGRRTESGV
CCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHCCCCCEECCCCCCCCCCCCCCCC
NLHCTWCGSHLPPWRCPECGSTEVRSGIVGATTTAAELGRAFPDVPVVDSSGNHVRERVD
EEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCEECCCCHHHHHHHC
STPRLIVATPGAEPTPESGYACAVILDAVSSLSRPGLAAVEQTVDRWMAILALVRSVRDG
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GRAVIVGPSEDAALQAMVRNDPVGWATRELADRREAHFPPAVPCGIVDGDPKAVATAAQR
CCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LREWFGTDLEMLGPAPQPRRASESERDRIIVRPRGTMPLAELSQGLFRLRSKHTMSREPG
HHHHHCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
SLRVVIDPANLL
CEEEEECCCCCC
>Mature Secondary Structure
MCPMTAPLLETTSWIAHVAVSSGLAHLDRPFDYLIPDDLVDRVQVGVRVRVKLAGRLCDG
CCCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHCCEEEEEEEECCCCCC
VVVAVDHEQQPGVTLAPVEKLVSDQVVATPDQLNFARHVADHWAGTLDEVLRWAIPTRHA
EEEEEECCCCCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TTETANPPPWPTPMTTMPSGTLTALDRGRRFLALLKAGERPRAHWRVSPVCGPGDDDHLG
CCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCC
DWGNGLLQAVCATLTSGRSAVVCCPTVDDVITLHERFQAVLGAGTVAVLHADQPASTRWR
CHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
HYLAVIRGTAKVVVGTRSAVMAPMTDLGLIAVWDEGSDLHDEPRAPYPNTRDVAALRAQI
HHHHHHHCCEEEEEECCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
DHCALLLASYSCSVRSASWLGSGWLVGIGADRPTMRAVCAPVRIPGDSDIAMERDPLARS
HHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCEECCCCCCCCCCCCCHHHH
VRLPDLAMTTIRDGLLHGPVLVSVPRVGDLVTPSCARCRTPVRCSSCGGPVTGRRTESGV
CCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHCCCCCEECCCCCCCCCCCCCCCC
NLHCTWCGSHLPPWRCPECGSTEVRSGIVGATTTAAELGRAFPDVPVVDSSGNHVRERVD
EEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCEECCCCHHHHHHHC
STPRLIVATPGAEPTPESGYACAVILDAVSSLSRPGLAAVEQTVDRWMAILALVRSVRDG
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GRAVIVGPSEDAALQAMVRNDPVGWATRELADRREAHFPPAVPCGIVDGDPKAVATAAQR
CCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
LREWFGTDLEMLGPAPQPRRASESERDRIIVRPRGTMPLAELSQGLFRLRSKHTMSREPG
HHHHHCCCHHHCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
SLRVVIDPANLL
CEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]