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The map label for this gene is miaA

Identifier: 50842502

GI number: 50842502

Start: 1103263

End: 1104222

Strand: Direct

Name: miaA

Synonym: PPA1019

Alternate gene names: 50842502

Gene position: 1103263-1104222 (Clockwise)

Preceding gene: 50842500

Following gene: 50842503

Centisome position: 43.09

GC content: 58.75

Gene sequence:

>960_bases
GTGCGCAAAAATGAATATGTGAATTTGCCGGTCATCTGCCTCGTCGGGCCCACTGCTAGCGGAAAATCAGGGCTAGCGGT
GCGAGTGTGCCGCCGCTTGTATGTCGATGAGCACCCCGCCGAAATTATTAATACTGACTCGATGGTGGTGTATCGCGGGA
TGGACATTGGCACTGCCACCCCTACACTTCGCGAGCAGCGCACGGTAGTGCATCACCTGGTGTCGATTCTTGATGTGACT
GTGCCCTCCTCGCTAGTACTGATGCAGACGCTGGCCCGTGATGCCGTCGAGGATTGTCTGTCGCGTGGTGTCATCCCTGT
CTTGGTGGGAGGGTCTGCGCTGTACACCAAGGCCATCATTGACGAAATGTCCATCCCGCCAACTGATCCGGAAGTGAGGG
CTCGGTGGCAGGAGAAGCTAGATGCCGAGGGGCCGCGAGTTCTGCATGACGAGCTTGCCCGTCGCGATCCCAAGGCGGCT
GAGTCAATCTTGCCCGGCAACGGCAGGCGAATCGTTCGTGCCCTCGAAGTTATTGACCTGACAGGGTCCTTTACTGCCAC
GATCCCCGACGGGACCCTCCACTGGCCTAAGACGGTGCAAATGGGCTTAGAACTGTCGCGCAAAGACATAGACCAGCGTA
TTGCCGATCGGGTTGACCAGATGTGGGCATACGGTTTCGTCGACGAAGTGCGTTCCCTAGCTAACGTCGGTCTCCGCGAA
GGGCTGACGGCGAGCCGTGCGTTGGGGTACCGCCAGGTGCTGGAATATCTGAATGGGGACTACGACGAGGATGAGGCCCG
TCGTAGGACCATCATTGGCACTCGTCGCTTCGCCCGTAAACAGCTCATGTGGTACCGCCGTGACGATCGCATTGAGTGGT
TCAATGCCCTCGCCCCAGACCTTGATGACCGGGTTGTGGCGAGGGTGTTGACCGCCCTGACGACCGACGAGGAGGATTGA

Upstream 100 bases:

>100_bases
TTTTCCCCCTCTCGGGGATGACTCTTGCAAGGCTACACATGGGATAGTCCACCCTTGCGAGGTTTTTCGCTCTACGCGTT
GTTGTAAGGATGTCGCGACG

Downstream 100 bases:

>100_bases
CGCTATGACGACCTTCTGGAAAGGACACGGTACCGGCAATGACTTCGTCATCGTCGACCAGCAGGACCCGCTGACTTCCG
CGCAGGTGCGTTGGCTGTGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRKNEYVNLPVICLVGPTASGKSGLAVRVCRRLYVDEHPAEIINTDSMVVYRGMDIGTATPTLREQRTVVHHLVSILDVT
VPSSLVLMQTLARDAVEDCLSRGVIPVLVGGSALYTKAIIDEMSIPPTDPEVRARWQEKLDAEGPRVLHDELARRDPKAA
ESILPGNGRRIVRALEVIDLTGSFTATIPDGTLHWPKTVQMGLELSRKDIDQRIADRVDQMWAYGFVDEVRSLANVGLRE
GLTASRALGYRQVLEYLNGDYDEDEARRRTIIGTRRFARKQLMWYRRDDRIEWFNALAPDLDDRVVARVLTALTTDEED

Sequences:

>Translated_319_residues
MRKNEYVNLPVICLVGPTASGKSGLAVRVCRRLYVDEHPAEIINTDSMVVYRGMDIGTATPTLREQRTVVHHLVSILDVT
VPSSLVLMQTLARDAVEDCLSRGVIPVLVGGSALYTKAIIDEMSIPPTDPEVRARWQEKLDAEGPRVLHDELARRDPKAA
ESILPGNGRRIVRALEVIDLTGSFTATIPDGTLHWPKTVQMGLELSRKDIDQRIADRVDQMWAYGFVDEVRSLANVGLRE
GLTASRALGYRQVLEYLNGDYDEDEARRRTIIGTRRFARKQLMWYRRDDRIEWFNALAPDLDDRVVARVLTALTTDEED
>Mature_319_residues
MRKNEYVNLPVICLVGPTASGKSGLAVRVCRRLYVDEHPAEIINTDSMVVYRGMDIGTATPTLREQRTVVHHLVSILDVT
VPSSLVLMQTLARDAVEDCLSRGVIPVLVGGSALYTKAIIDEMSIPPTDPEVRARWQEKLDAEGPRVLHDELARRDPKAA
ESILPGNGRRIVRALEVIDLTGSFTATIPDGTLHWPKTVQMGLELSRKDIDQRIADRVDQMWAYGFVDEVRSLANVGLRE
GLTASRALGYRQVLEYLNGDYDEDEARRRTIIGTRRFARKQLMWYRRDDRIEWFNALAPDLDDRVVARVLTALTTDEED

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=317, Percent_Identity=26.813880126183, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI1790613, Length=318, Percent_Identity=37.4213836477987, Blast_Score=177, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI25144712, Length=298, Percent_Identity=24.8322147651007, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=26.0135135135135, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24649830, Length=282, Percent_Identity=28.7234042553192, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PROAC (Q6A8Z5)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055729.1
- ProteinModelPortal:   Q6A8Z5
- SMR:   Q6A8Z5
- GeneID:   2932162
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1019
- NMPDR:   fig|267747.1.peg.1006
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   PACN267747:PPA1019-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35934; Mature: 35934

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNEYVNLPVICLVGPTASGKSGLAVRVCRRLYVDEHPAEIINTDSMVVYRGMDIGTAT
CCCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCCCC
PTLREQRTVVHHLVSILDVTVPSSLVLMQTLARDAVEDCLSRGVIPVLVGGSALYTKAII
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
DEMSIPPTDPEVRARWQEKLDAEGPRVLHDELARRDPKAAESILPGNGRRIVRALEVIDL
HHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHC
TGSFTATIPDGTLHWPKTVQMGLELSRKDIDQRIADRVDQMWAYGFVDEVRSLANVGLRE
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTASRALGYRQVLEYLNGDYDEDEARRRTIIGTRRFARKQLMWYRRDDRIEWFNALAPD
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
LDDRVVARVLTALTTDEED
CHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKNEYVNLPVICLVGPTASGKSGLAVRVCRRLYVDEHPAEIINTDSMVVYRGMDIGTAT
CCCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCCCC
PTLREQRTVVHHLVSILDVTVPSSLVLMQTLARDAVEDCLSRGVIPVLVGGSALYTKAII
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
DEMSIPPTDPEVRARWQEKLDAEGPRVLHDELARRDPKAAESILPGNGRRIVRALEVIDL
HHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHC
TGSFTATIPDGTLHWPKTVQMGLELSRKDIDQRIADRVDQMWAYGFVDEVRSLANVGLRE
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTASRALGYRQVLEYLNGDYDEDEARRRTIIGTRRFARKQLMWYRRDDRIEWFNALAPD
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
LDDRVVARVLTALTTDEED
CHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA