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The map label for this gene is miaB

Identifier: 50842500

GI number: 50842500

Start: 1101309

End: 1102793

Strand: Direct

Name: miaB

Synonym: PPA1017

Alternate gene names: 50842500

Gene position: 1101309-1102793 (Clockwise)

Preceding gene: 50842499

Following gene: 50842502

Centisome position: 43.02

GC content: 57.78

Gene sequence:

>1485_bases
ATGCCCAATAATACCTTGCAGCACACCGAGGCTCCGACTTATCGGGTCGTGACCTACGGGTGTCAGATGAATGTCCATGA
CTCCGAGCGCATCGCCGGGCTTCTCGAGGAGGCCGGCTACGTTCCCGATCCTAGAACTGACACCTTGGCGCCAGCTGATG
TCGTCGTCTTCAACACCTGCGCGGTGCGAGAGAACGCTGATAATCGTCTTTACGGCACCCTCGGCCACATGGCTGCAGTA
AAGTCGGCCCATCCTGGGATGCAGCTAGCTGTCGGCGGCTGCATGGCTCAGAAGGACAAGGACACCGTGGTTGCGCGAGC
TCCGTGGGTTGACGTCGTTTTTGGTACCCACAACGTTGGTTCGTTGCCGGTTCTACTTAAGAGAGCTCGCCACAATGCAA
CAGCTCAGGTGGAGATTGAGGAGTCTCTGGTCACCTTTCCATCGAATTTGCCTGCCAGGCGCGATTCCGCCTACTCAGCG
TGGGTGTCCATCTCGGTGGGTTGCAATAATACGTGTACTTTTTGCATCGTCCCGCAGTTGCGTGGCAAAGAAACTGACCG
CCGTCCCGGCGAGATCCTGTCTGAGATCCGTGCCCTCGTTAACGAGGGAGTCCAGGAGATTACGCTACTAGGCCAAAACG
TCAATTCCTATGGAGTTCAATTCGGCGATCGTGGCGCTTTTGCTAAGCTGCTTCGCGCCTGCGGAAACATCGACGGCCTA
GAGAGGGTGAGGTTCACCTCGCCGCATCCGGCCGCTTTTACCGACGACGTTATCGAGGCTATGGCTGAGACTCCTAACGT
CATGCCAAGCTTGCACATGCCGCTGCAGTCCGGTTCTGACGAGGTGCTGCGTCGGATGCGCCGTTCGTATCGATCCACGA
AGTTCCTGGGCATCCTCGACCGGGTGCGCGAGGCCATCCCTCACGCTGCTATTACTACTGATATCATTGTCGGTTTTCCG
GGTGAAACCGACAAAGATTTCGCCGAGACGATGCGAATCGTCGAGGAGTCGAGATTCTCTGCGGCTTTCACGTTCCAATA
TTCCATCCGTCCTAACACTCCGGCTGGCGTCATGAAGGATCAGGTTCCAAAACCGGTCGTCCAGGAGCGCTACGAGCAAC
TTGTCGAGCTCGTTGACGACATTGCTTGGCAAGAGAACAAGGCCCAGGTTGGACGGGCTGTCGAGGTCATGTTTGCGCAA
GGGGAGGGTCGCAAGGACGAAGCTACCCATCGCGTTACGGGCCGTGCCCGTGACAACCGCTTGGTTCACGTCAGTCTTAA
CCCCGGTGATGATGTGCCGCGTCCCGGTGATATCGGTGAGGTTGTTATTACGTCTGGTCACCCGCATCACTTGGTCGCTG
ATGGTGGCTTGGTGAATTTACGTCGTACTCGCGGGGGAGACGCGTGGCACGCCCGTCAGGGGCAGGTGTCCCAGGGTACT
CCTACGGTGCTCGGGCTTCCCACCGTGGGAGTGCCGAGACACTGA

Upstream 100 bases:

>100_bases
CTATGGCGGGATACTGCTCGTGCTTCAGACGGTGGTGTGATCGACACGTTCGTCAGCGAGTACACGTTGCGACGGGTACA
TGACGTAGAGTGTGTGCGCC

Downstream 100 bases:

>100_bases
TCGATGGGTCTTACAGGTATGGCATTTTACGGACAGTGAGGAAGATAGACGAGGGATGGCGCTCAATGGAGCACCACCCC
TCGTGTTGCAGCACTTCCGC

Product: MiaB family protein

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MPNNTLQHTEAPTYRVVTYGCQMNVHDSERIAGLLEEAGYVPDPRTDTLAPADVVVFNTCAVRENADNRLYGTLGHMAAV
KSAHPGMQLAVGGCMAQKDKDTVVARAPWVDVVFGTHNVGSLPVLLKRARHNATAQVEIEESLVTFPSNLPARRDSAYSA
WVSISVGCNNTCTFCIVPQLRGKETDRRPGEILSEIRALVNEGVQEITLLGQNVNSYGVQFGDRGAFAKLLRACGNIDGL
ERVRFTSPHPAAFTDDVIEAMAETPNVMPSLHMPLQSGSDEVLRRMRRSYRSTKFLGILDRVREAIPHAAITTDIIVGFP
GETDKDFAETMRIVEESRFSAAFTFQYSIRPNTPAGVMKDQVPKPVVQERYEQLVELVDDIAWQENKAQVGRAVEVMFAQ
GEGRKDEATHRVTGRARDNRLVHVSLNPGDDVPRPGDIGEVVITSGHPHHLVADGGLVNLRRTRGGDAWHARQGQVSQGT
PTVLGLPTVGVPRH

Sequences:

>Translated_494_residues
MPNNTLQHTEAPTYRVVTYGCQMNVHDSERIAGLLEEAGYVPDPRTDTLAPADVVVFNTCAVRENADNRLYGTLGHMAAV
KSAHPGMQLAVGGCMAQKDKDTVVARAPWVDVVFGTHNVGSLPVLLKRARHNATAQVEIEESLVTFPSNLPARRDSAYSA
WVSISVGCNNTCTFCIVPQLRGKETDRRPGEILSEIRALVNEGVQEITLLGQNVNSYGVQFGDRGAFAKLLRACGNIDGL
ERVRFTSPHPAAFTDDVIEAMAETPNVMPSLHMPLQSGSDEVLRRMRRSYRSTKFLGILDRVREAIPHAAITTDIIVGFP
GETDKDFAETMRIVEESRFSAAFTFQYSIRPNTPAGVMKDQVPKPVVQERYEQLVELVDDIAWQENKAQVGRAVEVMFAQ
GEGRKDEATHRVTGRARDNRLVHVSLNPGDDVPRPGDIGEVVITSGHPHHLVADGGLVNLRRTRGGDAWHARQGQVSQGT
PTVLGLPTVGVPRH
>Mature_493_residues
PNNTLQHTEAPTYRVVTYGCQMNVHDSERIAGLLEEAGYVPDPRTDTLAPADVVVFNTCAVRENADNRLYGTLGHMAAVK
SAHPGMQLAVGGCMAQKDKDTVVARAPWVDVVFGTHNVGSLPVLLKRARHNATAQVEIEESLVTFPSNLPARRDSAYSAW
VSISVGCNNTCTFCIVPQLRGKETDRRPGEILSEIRALVNEGVQEITLLGQNVNSYGVQFGDRGAFAKLLRACGNIDGLE
RVRFTSPHPAAFTDDVIEAMAETPNVMPSLHMPLQSGSDEVLRRMRRSYRSTKFLGILDRVREAIPHAAITTDIIVGFPG
ETDKDFAETMRIVEESRFSAAFTFQYSIRPNTPAGVMKDQVPKPVVQERYEQLVELVDDIAWQENKAQVGRAVEVMFAQG
EGRKDEATHRVTGRARDNRLVHVSLNPGDDVPRPGDIGEVVITSGHPHHLVADGGLVNLRRTRGGDAWHARQGQVSQGTP
TVLGLPTVGVPRH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=34.8837209302326, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI28872782, Length=470, Percent_Identity=34.8936170212766, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI93277076, Length=377, Percent_Identity=30.7692307692308, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1786882, Length=439, Percent_Identity=37.129840546697, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1787057, Length=406, Percent_Identity=30.2955665024631, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=32.1276595744681, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=218, Percent_Identity=34.4036697247706, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356207, Length=425, Percent_Identity=36.2352941176471, Blast_Score=275, Evalue=8e-74,
Organism=Drosophila melanogaster, GI19922432, Length=446, Percent_Identity=28.47533632287, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PROAC (Q6A8Z7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055727.1
- ProteinModelPortal:   Q6A8Z7
- SMR:   Q6A8Z7
- GeneID:   2931426
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1017
- NMPDR:   fig|267747.1.peg.1004
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   CLSK2763673
- BioCyc:   PACN267747:PPA1017-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 54112; Mature: 53981

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNTLQHTEAPTYRVVTYGCQMNVHDSERIAGLLEEAGYVPDPRTDTLAPADVVVFNTC
CCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
AVRENADNRLYGTLGHMAAVKSAHPGMQLAVGGCMAQKDKDTVVARAPWVDVVFGTHNVG
EECCCCCCCEEEHHHHHHHHHHCCCCCEEEECCHHCCCCCCCEEEECCEEEEEECCCCCC
SLPVLLKRARHNATAQVEIEESLVTFPSNLPARRDSAYSAWVSISVGCNNTCTFCIVPQL
CHHHHHHHHCCCCEEEEEEHHHHEECCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCC
RGKETDRRPGEILSEIRALVNEGVQEITLLGQNVNSYGVQFGDRGAFAKLLRACGNIDGL
CCCCCCCCHHHHHHHHHHHHHCCHHEEHHHCCCCHHHCCCCCCCHHHHHHHHHHCCCCCH
ERVRFTSPHPAAFTDDVIEAMAETPNVMPSLHMPLQSGSDEVLRRMRRSYRSTKFLGILD
HHEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RVREAIPHAAITTDIIVGFPGETDKDFAETMRIVEESRFSAAFTFQYSIRPNTPAGVMKD
HHHHHCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHH
QVPKPVVQERYEQLVELVDDIAWQENKAQVGRAVEVMFAQGEGRKDEATHRVTGRARDNR
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEECCCCCCCCCHHHCCCCCCCCE
LVHVSLNPGDDVPRPGDIGEVVITSGHPHHLVADGGLVNLRRTRGGDAWHARQGQVSQGT
EEEEEECCCCCCCCCCCCCCEEEECCCCCEEEECCCEEEEEECCCCCCEECCCCCCCCCC
PTVLGLPTVGVPRH
CCEEECCCCCCCCC
>Mature Secondary Structure 
PNNTLQHTEAPTYRVVTYGCQMNVHDSERIAGLLEEAGYVPDPRTDTLAPADVVVFNTC
CCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
AVRENADNRLYGTLGHMAAVKSAHPGMQLAVGGCMAQKDKDTVVARAPWVDVVFGTHNVG
EECCCCCCCEEEHHHHHHHHHHCCCCCEEEECCHHCCCCCCCEEEECCEEEEEECCCCCC
SLPVLLKRARHNATAQVEIEESLVTFPSNLPARRDSAYSAWVSISVGCNNTCTFCIVPQL
CHHHHHHHHCCCCEEEEEEHHHHEECCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCC
RGKETDRRPGEILSEIRALVNEGVQEITLLGQNVNSYGVQFGDRGAFAKLLRACGNIDGL
CCCCCCCCHHHHHHHHHHHHHCCHHEEHHHCCCCHHHCCCCCCCHHHHHHHHHHCCCCCH
ERVRFTSPHPAAFTDDVIEAMAETPNVMPSLHMPLQSGSDEVLRRMRRSYRSTKFLGILD
HHEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RVREAIPHAAITTDIIVGFPGETDKDFAETMRIVEESRFSAAFTFQYSIRPNTPAGVMKD
HHHHHCCCHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHH
QVPKPVVQERYEQLVELVDDIAWQENKAQVGRAVEVMFAQGEGRKDEATHRVTGRARDNR
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEEEECCCCCCCCCHHHCCCCCCCCE
LVHVSLNPGDDVPRPGDIGEVVITSGHPHHLVADGGLVNLRRTRGGDAWHARQGQVSQGT
EEEEEECCCCCCCCCCCCCCEEEECCCCCEEEECCCEEEEEECCCCCCEECCCCCCCCCC
PTVLGLPTVGVPRH
CCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA