Click here to switch to the map view.

The map label for this gene is dapF

Identifier: 50842503

GI number: 50842503

Start: 1104227

End: 1105048

Strand: Direct

Name: dapF

Synonym: PPA1020

Alternate gene names: 50842503

Gene position: 1104227-1105048 (Clockwise)

Preceding gene: 50842502

Following gene: 50842504

Centisome position: 43.13

GC content: 60.1

Gene sequence:

>822_bases
ATGACGACCTTCTGGAAAGGACACGGTACCGGCAATGACTTCGTCATCGTCGACCAGCAGGACCCGCTGACTTCCGCGCA
GGTGCGTTGGCTGTGCCATCGCCGATTCGGGATCGGGGCCGACGGTACCTTGCAAGCTCTGCGAGCCGGAGATGTTGAGG
ACTGGAATGGCGATCCTGACCTGTGGTTCATGGATTATCGCAACGCTGATGGCACGATCGCTGAAATGTGTGGCAACGGT
TTGCGTGTCTTCGCACGCTATCTCGTCCAGACCGGGCGCATGACGACTACTGAGGCTGACGTCGCGACGCGCGCTGGGGT
GCGCCATGTCAGACTGCATGACGGTGGCGATGTGTCCGTGACCATGGGAACAGTCCGGGTTGAGTCCGGCAAGGTGACCA
TCGAGCATCAAGATATGTGGTGGCCGGCGTGCAAAGTTGACGTGGGCAACCCGCATGCTGTTGTTGTGACCGACCACACC
ACAGTCTGTGGTATTAACCTTAATGACTCACCGACTTGGCGGCCACGATCTGCATTCCCTGATGGCGTCAACGTCGAATT
TGTCGAGCAGATAGACCAGCACACCGTGGCGATGCGAGTCCATGAGAGGGGCTCGGGGGAGACGATGTCTTGCGGTACGG
GGACGGTGGCAGTTGCTGCAACCATGGCCACTCGCACCGGACATCGAGGACCGTGGACCGTCCAAGTTCCAGGTGGGCGA
GTCGTCGTCACGCTGAGCCCTTCCGATGACGGCTACCGGGCTATCCTTACCGGTCCTGCCGTGATCCTGGCCGCTGGCAA
GGTGGATCTGCCGAATGGATGA

Upstream 100 bases:

>100_bases
GATCGCATTGAGTGGTTCAATGCCCTCGCCCCAGACCTTGATGACCGGGTTGTGGCGAGGGTGTTGACCGCCCTGACGAC
CGACGAGGAGGATTGACGCT

Downstream 100 bases:

>100_bases
GAGGGCGAACACGCTCTTGTCAACCTGTCGGAACGTCCTCCTACACTTAGCGCGATGGCATTGGGGCGTGAGGATGGGTA
CCGGGTGAGCGAGATCGAAC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTTFWKGHGTGNDFVIVDQQDPLTSAQVRWLCHRRFGIGADGTLQALRAGDVEDWNGDPDLWFMDYRNADGTIAEMCGNG
LRVFARYLVQTGRMTTTEADVATRAGVRHVRLHDGGDVSVTMGTVRVESGKVTIEHQDMWWPACKVDVGNPHAVVVTDHT
TVCGINLNDSPTWRPRSAFPDGVNVEFVEQIDQHTVAMRVHERGSGETMSCGTGTVAVAATMATRTGHRGPWTVQVPGGR
VVVTLSPSDDGYRAILTGPAVILAAGKVDLPNG

Sequences:

>Translated_273_residues
MTTFWKGHGTGNDFVIVDQQDPLTSAQVRWLCHRRFGIGADGTLQALRAGDVEDWNGDPDLWFMDYRNADGTIAEMCGNG
LRVFARYLVQTGRMTTTEADVATRAGVRHVRLHDGGDVSVTMGTVRVESGKVTIEHQDMWWPACKVDVGNPHAVVVTDHT
TVCGINLNDSPTWRPRSAFPDGVNVEFVEQIDQHTVAMRVHERGSGETMSCGTGTVAVAATMATRTGHRGPWTVQVPGGR
VVVTLSPSDDGYRAILTGPAVILAAGKVDLPNG
>Mature_272_residues
TTFWKGHGTGNDFVIVDQQDPLTSAQVRWLCHRRFGIGADGTLQALRAGDVEDWNGDPDLWFMDYRNADGTIAEMCGNGL
RVFARYLVQTGRMTTTEADVATRAGVRHVRLHDGGDVSVTMGTVRVESGKVTIEHQDMWWPACKVDVGNPHAVVVTDHTT
VCGINLNDSPTWRPRSAFPDGVNVEFVEQIDQHTVAMRVHERGSGETMSCGTGTVAVAATMATRTGHRGPWTVQVPGGRV
VVTLSPSDDGYRAILTGPAVILAAGKVDLPNG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=283, Percent_Identity=31.095406360424, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29522; Mature: 29391

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFWKGHGTGNDFVIVDQQDPLTSAQVRWLCHRRFGIGADGTLQALRAGDVEDWNGDPD
CCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCC
LWFMDYRNADGTIAEMCGNGLRVFARYLVQTGRMTTTEADVATRAGVRHVRLHDGGDVSV
EEEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCCEECHHHHHHHCCEEEEEEECCCCEEE
TMGTVRVESGKVTIEHQDMWWPACKVDVGNPHAVVVTDHTTVCGINLNDSPTWRPRSAFP
EEEEEEEECCEEEEEECCCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCC
DGVNVEFVEQIDQHTVAMRVHERGSGETMSCGTGTVAVAATMATRTGHRGPWTVQVPGGR
CCCCHHHHHHHHHCEEEEEEEECCCCCEEECCCCCEEEEEEEHHCCCCCCCEEEEECCCE
VVVTLSPSDDGYRAILTGPAVILAAGKVDLPNG
EEEEECCCCCCCEEEEECCEEEEEECEEECCCC
>Mature Secondary Structure 
TTFWKGHGTGNDFVIVDQQDPLTSAQVRWLCHRRFGIGADGTLQALRAGDVEDWNGDPD
CEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCC
LWFMDYRNADGTIAEMCGNGLRVFARYLVQTGRMTTTEADVATRAGVRHVRLHDGGDVSV
EEEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCCEECHHHHHHHCCEEEEEEECCCCEEE
TMGTVRVESGKVTIEHQDMWWPACKVDVGNPHAVVVTDHTTVCGINLNDSPTWRPRSAFP
EEEEEEEECCEEEEEECCCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCC
DGVNVEFVEQIDQHTVAMRVHERGSGETMSCGTGTVAVAATMATRTGHRGPWTVQVPGGR
CCCCHHHHHHHHHCEEEEEEEECCCCCEEECCCCCEEEEEEEHHCCCCCCCEEEEECCCE
VVVTLSPSDDGYRAILTGPAVILAAGKVDLPNG
EEEEECCCCCCCEEEEECCEEEEEECEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]