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The map label for this gene is bioB [H]

Identifier: 50842451

GI number: 50842451

Start: 1051546

End: 1052817

Strand: Direct

Name: bioB [H]

Synonym: PPA0967

Alternate gene names: 50842451

Gene position: 1051546-1052817 (Clockwise)

Preceding gene: 50842450

Following gene: 50842452

Centisome position: 41.07

GC content: 61.4

Gene sequence:

>1272_bases
ATGCTTGCAGTGACGGCTGCCAACGGAGTCCGGCTGCAGCTGCATGATGGGGAACACCACCACGAGGTCATCGATGCGAT
GGCCTCGTGGTGGTGCCAGATTCACGGTTACCGAAACCCGGTCCTCGACGAGGCCCTCAACCGTCAAAGCTCCCAGTTCA
GTCATGTCATGTTTGGCGGACTCACCCATAAGGCCGCGGTTGACGCCGTCATGTCCCTAGTGCGCCTGGCCCCGGGGCCC
CTCGACCGGATCTTCCTGGCTGATTCCGGGTCTGTCGGCGTCGAGGTGAGTCTCAAATTGGCTCGTCAGGTGCAAATCGC
TCGCACCGCAGCGCGCGGCGGCACTTTGACGAGGACACGCGTTGCCGCTCTGCGAGGCGCCTATCACGGTGACACTCTCG
GTGCTATGAGCGTGTGCGACCCTATCGGTGGCATGCACGCCATGTTCAGCGACTCTATTCCCCAGCAGATCTTCCTGCCG
CGCCCTCCTTCTTTCGCCGCCGACGAGGCCGACGTGGAGACGTGGTGCAGCGAGGCCGATGAAATCCTGGACACCCACCG
CGACGACCTGGCCGGGATCATTGTCGAGCCCATCTTGCAAGGAGCCGGAGGCATGTGGCCGTGGTCTCCGTCCTGTCTGA
AGCACCTGCGCCGTCGTGCTGATGAACTTGACCTAGTTCTTATCGCCGACGAGGTCGCTACTGGATTTGGGCGGACTGGC
AAACTTTTCGCATGCGAGTGGGCCGATATCGTTCCTGACATCATGGTGGTTGGGAAATCCATGACTGGCGGATACCTGAC
CCAGTCGGCCGTGCTGTGTTCCGACGAGCTGGCCAGTGAGGTCAGCCGAGGCCCAGGCGGATCCCTTATGCACGGCCCCA
CTTTCATGGGAAACCCTTTGGCCAGTGCCGTCGCTGCAGCGAGCCTAGCGATCATCTCCCAGGGACAGTGGAACGACGAC
GTCACCCGCATCGCCGATGTGATGTCCAGGGAGCTAGCGTCACTGCGCAATGTGCCCGGGGTCATCGACGTCCGCGTCTT
CGGAGCTATCGCCGCGGTCCAGGTCGACACCCCGATTGTCATGCGACGGGCGACTCGCACTGCCGTCGAGAACGGGGTGT
GGCTTCGACCCTTCCGTGATCTCGTCTACGCCATGCCGCCCTATATCTGTACCAACGATGATCTGGAGGCCATGGCCGCT
GGGATGCGTGCCGTTGTCATAGATCAACTTGATGCTGCTCAGGACAATACCGATTATCGGAAGGAATCATGA

Upstream 100 bases:

>100_bases
GCGGCTAGCATTCCTCGCTGTGAGTCCTGAACAGCAGCTTCTCGAATATGACCGACGTCATGTCTGGCACCCCTACGCAC
CGACGATCGGGGCAGACCCA

Downstream 100 bases:

>100_bases
ACATTACCGATCTCAACGGAATTGTCATCGTTACGGGAACTGACACCGACGTCGGCAAGACAGTCACTACTGCGGTCATC
GCCTCAGCACTCAAGCAATC

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MLAVTAANGVRLQLHDGEHHHEVIDAMASWWCQIHGYRNPVLDEALNRQSSQFSHVMFGGLTHKAAVDAVMSLVRLAPGP
LDRIFLADSGSVGVEVSLKLARQVQIARTAARGGTLTRTRVAALRGAYHGDTLGAMSVCDPIGGMHAMFSDSIPQQIFLP
RPPSFAADEADVETWCSEADEILDTHRDDLAGIIVEPILQGAGGMWPWSPSCLKHLRRRADELDLVLIADEVATGFGRTG
KLFACEWADIVPDIMVVGKSMTGGYLTQSAVLCSDELASEVSRGPGGSLMHGPTFMGNPLASAVAAASLAIISQGQWNDD
VTRIADVMSRELASLRNVPGVIDVRVFGAIAAVQVDTPIVMRRATRTAVENGVWLRPFRDLVYAMPPYICTNDDLEAMAA
GMRAVVIDQLDAAQDNTDYRKES

Sequences:

>Translated_423_residues
MLAVTAANGVRLQLHDGEHHHEVIDAMASWWCQIHGYRNPVLDEALNRQSSQFSHVMFGGLTHKAAVDAVMSLVRLAPGP
LDRIFLADSGSVGVEVSLKLARQVQIARTAARGGTLTRTRVAALRGAYHGDTLGAMSVCDPIGGMHAMFSDSIPQQIFLP
RPPSFAADEADVETWCSEADEILDTHRDDLAGIIVEPILQGAGGMWPWSPSCLKHLRRRADELDLVLIADEVATGFGRTG
KLFACEWADIVPDIMVVGKSMTGGYLTQSAVLCSDELASEVSRGPGGSLMHGPTFMGNPLASAVAAASLAIISQGQWNDD
VTRIADVMSRELASLRNVPGVIDVRVFGAIAAVQVDTPIVMRRATRTAVENGVWLRPFRDLVYAMPPYICTNDDLEAMAA
GMRAVVIDQLDAAQDNTDYRKES
>Mature_423_residues
MLAVTAANGVRLQLHDGEHHHEVIDAMASWWCQIHGYRNPVLDEALNRQSSQFSHVMFGGLTHKAAVDAVMSLVRLAPGP
LDRIFLADSGSVGVEVSLKLARQVQIARTAARGGTLTRTRVAALRGAYHGDTLGAMSVCDPIGGMHAMFSDSIPQQIFLP
RPPSFAADEADVETWCSEADEILDTHRDDLAGIIVEPILQGAGGMWPWSPSCLKHLRRRADELDLVLIADEVATGFGRTG
KLFACEWADIVPDIMVVGKSMTGGYLTQSAVLCSDELASEVSRGPGGSLMHGPTFMGNPLASAVAAASLAIISQGQWNDD
VTRIADVMSRELASLRNVPGVIDVRVFGAIAAVQVDTPIVMRRATRTAVENGVWLRPFRDLVYAMPPYICTNDDLEAMAA
GMRAVVIDQLDAAQDNTDYRKES

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=398, Percent_Identity=28.643216080402, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI4557809, Length=400, Percent_Identity=24.75, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI284507298, Length=210, Percent_Identity=29.5238095238095, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI226442709, Length=377, Percent_Identity=25.4641909814324, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI37574042, Length=377, Percent_Identity=25.4641909814324, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI226442705, Length=377, Percent_Identity=25.4641909814324, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786991, Length=406, Percent_Identity=46.7980295566502, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI145693181, Length=348, Percent_Identity=32.183908045977, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788044, Length=343, Percent_Identity=32.3615160349854, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1789016, Length=336, Percent_Identity=30.0595238095238, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789759, Length=292, Percent_Identity=30.1369863013699, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787560, Length=346, Percent_Identity=30.635838150289, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786349, Length=402, Percent_Identity=25.3731343283582, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144271, Length=401, Percent_Identity=25.9351620947631, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=230, Percent_Identity=30, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32564660, Length=351, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324386, Length=428, Percent_Identity=41.3551401869159, Blast_Score=316, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6324432, Length=335, Percent_Identity=27.4626865671642, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323470, Length=396, Percent_Identity=26.5151515151515, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21357415, Length=412, Percent_Identity=25.7281553398058, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161085790, Length=360, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574759, Length=360, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 45597; Mature: 45597

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAVTAANGVRLQLHDGEHHHEVIDAMASWWCQIHGYRNPVLDEALNRQSSQFSHVMFGG
CEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
LTHKAAVDAVMSLVRLAPGPLDRIFLADSGSVGVEVSLKLARQVQIARTAARGGTLTRTR
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHH
VAALRGAYHGDTLGAMSVCDPIGGMHAMFSDSIPQQIFLPRPPSFAADEADVETWCSEAD
HHHHHCCCCCCCCCHHHHHCCCCCHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHH
EILDTHRDDLAGIIVEPILQGAGGMWPWSPSCLKHLRRRADELDLVLIADEVATGFGRTG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCC
KLFACEWADIVPDIMVVGKSMTGGYLTQSAVLCSDELASEVSRGPGGSLMHGPTFMGNPL
CEEEECHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
ASAVAAASLAIISQGQWNDDVTRIADVMSRELASLRNVPGVIDVRVFGAIAAVQVDTPIV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHEEEECCHHH
MRRATRTAVENGVWLRPFRDLVYAMPPYICTNDDLEAMAAGMRAVVIDQLDAAQDNTDYR
HHHHHHHHHHCCCEECHHHHHHHHCCCCEECCCCHHHHHHCCHHHHHHHHHCCCCCCCCC
KES
CCC
>Mature Secondary Structure
MLAVTAANGVRLQLHDGEHHHEVIDAMASWWCQIHGYRNPVLDEALNRQSSQFSHVMFGG
CEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
LTHKAAVDAVMSLVRLAPGPLDRIFLADSGSVGVEVSLKLARQVQIARTAARGGTLTRTR
CHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHH
VAALRGAYHGDTLGAMSVCDPIGGMHAMFSDSIPQQIFLPRPPSFAADEADVETWCSEAD
HHHHHCCCCCCCCCHHHHHCCCCCHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHH
EILDTHRDDLAGIIVEPILQGAGGMWPWSPSCLKHLRRRADELDLVLIADEVATGFGRTG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCC
KLFACEWADIVPDIMVVGKSMTGGYLTQSAVLCSDELASEVSRGPGGSLMHGPTFMGNPL
CEEEECHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
ASAVAAASLAIISQGQWNDDVTRIADVMSRELASLRNVPGVIDVRVFGAIAAVQVDTPIV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHEEEECCHHH
MRRATRTAVENGVWLRPFRDLVYAMPPYICTNDDLEAMAAGMRAVVIDQLDAAQDNTDYR
HHHHHHHHHHCCCEECHHHHHHHHCCCCEECCCCHHHHHHCCHHHHHHHHHCCCCCCCCC
KES
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA