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The map label for this gene is pdxT

Identifier: 50842450

GI number: 50842450

Start: 1050789

End: 1051394

Strand: Direct

Name: pdxT

Synonym: PPA0966

Alternate gene names: 50842450

Gene position: 1050789-1051394 (Clockwise)

Preceding gene: 50842449

Following gene: 50842451

Centisome position: 41.04

GC content: 62.38

Gene sequence:

>606_bases
ATGGCCCCGCTTGTCGGGGTGGTGGCCCTGCAGGGCGGGTTCGCCGAGCACATTGAGGTTCTCGAGTCGCTGGGGGCCAA
TACTCGTCGGGTTCGTCGGAGCGCTGATCTTCAGGGTCTCGACGGGATTGTCTTACCAGGCGGAGAGTCGACCGTTATTG
ACAAGCTCATGAGGTCGTTCAGCTTGGCGGAACCGCTTAAAGATGCCGTGCGCAGAGGCCTTCCGGTGCTGGCCACCTGT
GCTGGGCTCGTCGTGCTAGCTACTGATCTGGAGGACGCGGCAAAAGGCCAACACACCCTCAGCTTGCTCGACGTTACTGT
GCGTCGCAACGCCTTCGGGTCCCAGTTGGATTCCTTTGAGGGGACCCTCGACATCGACGGGGTTGGGGATGGGGTCTCGG
CTACCTTCATCCGGGCTCCCGTCATTACCCGGGTGGGACCCGGTGTCGAGGTCATTGCTCAGCTGCCTGACGAGGCAGGC
AACGTAAGCGGGGCCATCGTCGGCGTGAGGCAGAGGAACGTCCTCGCCCTGTCATTCCACCCTGAAGAGACTGACGACGA
CCGGGTACACCGCACCTGGTTGCGCCAGGTGTCTGAGGAAGTCTGA

Upstream 100 bases:

>100_bases
ACAATTGCCGAGGTTTCTCGTGGTTTGGGCGAGGCCATGGTCGGCATCAATGTCGCTGATGTACCAGCCCCGCACCGTCT
GGCTGAGCGAGGTTGGTGAG

Downstream 100 bases:

>100_bases
CAGTTACCGCAAGGGCTCATGACGACCCCTCCTGAACACTGTTCAAGGACGGCGGCTAGCATTCCTCGCTGTGAGTCCTG
AACAGCAGCTTCTCGAATAT

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MAPLVGVVALQGGFAEHIEVLESLGANTRRVRRSADLQGLDGIVLPGGESTVIDKLMRSFSLAEPLKDAVRRGLPVLATC
AGLVVLATDLEDAAKGQHTLSLLDVTVRRNAFGSQLDSFEGTLDIDGVGDGVSATFIRAPVITRVGPGVEVIAQLPDEAG
NVSGAIVGVRQRNVLALSFHPEETDDDRVHRTWLRQVSEEV

Sequences:

>Translated_201_residues
MAPLVGVVALQGGFAEHIEVLESLGANTRRVRRSADLQGLDGIVLPGGESTVIDKLMRSFSLAEPLKDAVRRGLPVLATC
AGLVVLATDLEDAAKGQHTLSLLDVTVRRNAFGSQLDSFEGTLDIDGVGDGVSATFIRAPVITRVGPGVEVIAQLPDEAG
NVSGAIVGVRQRNVLALSFHPEETDDDRVHRTWLRQVSEEV
>Mature_200_residues
APLVGVVALQGGFAEHIEVLESLGANTRRVRRSADLQGLDGIVLPGGESTVIDKLMRSFSLAEPLKDAVRRGLPVLATCA
GLVVLATDLEDAAKGQHTLSLLDVTVRRNAFGSQLDSFEGTLDIDGVGDGVSATFIRAPVITRVGPGVEVIAQLPDEAGN
VSGAIVGVRQRNVLALSFHPEETDDDRVHRTWLRQVSEEV

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=208, Percent_Identity=37.0192307692308, Blast_Score=115, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321048, Length=214, Percent_Identity=34.5794392523364, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323995, Length=214, Percent_Identity=33.6448598130841, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_PROAC (Q6A947)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055677.1
- ProteinModelPortal:   Q6A947
- SMR:   Q6A947
- GeneID:   2932790
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0966
- NMPDR:   fig|267747.1.peg.954
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- BioCyc:   PACN267747:PPA0966-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21325; Mature: 21193

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 80-80 ACT_SITE 180-180 ACT_SITE 182-182 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPLVGVVALQGGFAEHIEVLESLGANTRRVRRSADLQGLDGIVLPGGESTVIDKLMRSF
CCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHH
SLAEPLKDAVRRGLPVLATCAGLVVLATDLEDAAKGQHTLSLLDVTVRRNAFGSQLDSFE
HHHHHHHHHHHCCCCHHHHHHHHHHEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
GTLDIDGVGDGVSATFIRAPVITRVGPGVEVIAQLPDEAGNVSGAIVGVRQRNVLALSFH
CEEEECCCCCCCCHHHHHCCHHHHCCCCHHHHHHCCCCCCCCCEEEEEEECCCEEEEEEC
PEETDDDRVHRTWLRQVSEEV
CCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APLVGVVALQGGFAEHIEVLESLGANTRRVRRSADLQGLDGIVLPGGESTVIDKLMRSF
CCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHH
SLAEPLKDAVRRGLPVLATCAGLVVLATDLEDAAKGQHTLSLLDVTVRRNAFGSQLDSFE
HHHHHHHHHHHCCCCHHHHHHHHHHEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
GTLDIDGVGDGVSATFIRAPVITRVGPGVEVIAQLPDEAGNVSGAIVGVRQRNVLALSFH
CEEEECCCCCCCCHHHHHCCHHHHCCCCHHHHHHCCCCCCCCCEEEEEEECCCEEEEEEC
PEETDDDRVHRTWLRQVSEEV
CCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA