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The map label for this gene is tgt [H]

Identifier: 50842408

GI number: 50842408

Start: 1007244

End: 1008485

Strand: Direct

Name: tgt [H]

Synonym: PPA0924

Alternate gene names: 50842408

Gene position: 1007244-1008485 (Clockwise)

Preceding gene: 50842401

Following gene: 50842409

Centisome position: 39.34

GC content: 61.27

Gene sequence:

>1242_bases
ATGACATTTGGCTTCGAGGTCACCTCTCGTCTGGACAATACTTGTGGACGTACCGGCCTCATACACACCCCGCACGGTGT
CATCCAGACGCCGGCCTTCGTCGTCGTCGGCACCAAGGCGACGGTCAAGGCCCTGTTGCCCGAGTCAGTGGTTCAACTGG
GAGCTCAGGCGGTGCTCGCCAACGCCTACCACCTTTTCCTGCAGCCCGGTCCGGAGTTGATCGACGAGGCGGGCGGCCTG
GGCCGATTCATGAACTGGACGGGTCCCACCTTCACTGATTCCGGTGGCTTCCAGGTGCTCAGCCTTGGTGCGGGTTTCAA
GAAGACCCTAGCCATGGACGTTTCCCAGCTCACCGAGGCCGACGTCATTGCCGCTGATGCCGATCGTAAGGCGATGGTCG
ACGACGACGGTGTGACTTTTCGCAGTCCCCTCAATGGAGATCTACATCGTTTCACTCCCGAGGTGTCGATGGGCATCCAG
CACCACCTCGGCGCCGACATCATGTTCTCCTTCGACGAGCTGACCACCCTTATGAACACCCGCGCTTATCAGGAAGAAGC
CTTGGAGCGCACCCGGCGGTGGGCTGAGCGCTGCCTGGCCGAACATCGTCGTCTCACCGAGGTGCGCAGCGGCAAGCCGT
ACCAAGCCCTTTTTGGTGTCATCCAGGGAGCCCAGTACGAAGATTTGCGTCGTAGGGCTTGCCGGGATTTGGCAGGCATG
GAGATCGACGGTCACTGCTTCGACGGGTTCGGCATTGGCGGCGCCATCGAGAAAGCCAATCTGGGACGAATCGTGACGTG
GTGTGCTGAGGAGCTTCCCGAGGACCGTCCCCGTCACTTGTTGGGCATCTCCGAGCCCGACGACCTCTTCGCGGCCTGTC
GGGCGGGGGCCGATACCTTCGATTGTGTTAGCCCCTCCCGGGTGGCCCGTAACGCTGCCATCTACACCGTCGACGGGCGT
TACAACGTTGATACCGCGCGGTTCCGGCGAGACTTCGGCCCGTTGGAGGACGACTGCGACTGCTACACATGCACGCACTA
TTCCCGCGCCTATATCCACCACCTGTTCAAGGCTAAGGAGCTTCTCGCGAACACTTTGGCGACGATCCACAACGAGCGTT
GGACGGTAAGACTGGTCGACCAGATTCGGGGCGCCATGTGTAGCGGGGACCTCGATGCTTTCGAGACCGAGTTCATGGGG
CGGTGGAATGCCAACGGCGGGAGGCTGGCCAAGGTTAACTGA

Upstream 100 bases:

>100_bases
ATGAACGCCACCATAACGCCGTGGTTGCTAAGCCCTCACCCGGGCAGGCCTGCGAACCTGCGTGGTCGGTGGGTTCCGGA
CGTCCTGCAAGAATAACTCC

Downstream 100 bases:

>100_bases
CAGGGCTGTTCTCCGGAAACTCATCCTTACGACGGAGGAGACTGACAGTGCCACTCGTTACGCTCGGGGGCGTGCGAGAA
TCGATCGTGTCCTTCATGGC

Product: queuine/archaeosine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTFGFEVTSRLDNTCGRTGLIHTPHGVIQTPAFVVVGTKATVKALLPESVVQLGAQAVLANAYHLFLQPGPELIDEAGGL
GRFMNWTGPTFTDSGGFQVLSLGAGFKKTLAMDVSQLTEADVIAADADRKAMVDDDGVTFRSPLNGDLHRFTPEVSMGIQ
HHLGADIMFSFDELTTLMNTRAYQEEALERTRRWAERCLAEHRRLTEVRSGKPYQALFGVIQGAQYEDLRRRACRDLAGM
EIDGHCFDGFGIGGAIEKANLGRIVTWCAEELPEDRPRHLLGISEPDDLFAACRAGADTFDCVSPSRVARNAAIYTVDGR
YNVDTARFRRDFGPLEDDCDCYTCTHYSRAYIHHLFKAKELLANTLATIHNERWTVRLVDQIRGAMCSGDLDAFETEFMG
RWNANGGRLAKVN

Sequences:

>Translated_413_residues
MTFGFEVTSRLDNTCGRTGLIHTPHGVIQTPAFVVVGTKATVKALLPESVVQLGAQAVLANAYHLFLQPGPELIDEAGGL
GRFMNWTGPTFTDSGGFQVLSLGAGFKKTLAMDVSQLTEADVIAADADRKAMVDDDGVTFRSPLNGDLHRFTPEVSMGIQ
HHLGADIMFSFDELTTLMNTRAYQEEALERTRRWAERCLAEHRRLTEVRSGKPYQALFGVIQGAQYEDLRRRACRDLAGM
EIDGHCFDGFGIGGAIEKANLGRIVTWCAEELPEDRPRHLLGISEPDDLFAACRAGADTFDCVSPSRVARNAAIYTVDGR
YNVDTARFRRDFGPLEDDCDCYTCTHYSRAYIHHLFKAKELLANTLATIHNERWTVRLVDQIRGAMCSGDLDAFETEFMG
RWNANGGRLAKVN
>Mature_412_residues
TFGFEVTSRLDNTCGRTGLIHTPHGVIQTPAFVVVGTKATVKALLPESVVQLGAQAVLANAYHLFLQPGPELIDEAGGLG
RFMNWTGPTFTDSGGFQVLSLGAGFKKTLAMDVSQLTEADVIAADADRKAMVDDDGVTFRSPLNGDLHRFTPEVSMGIQH
HLGADIMFSFDELTTLMNTRAYQEEALERTRRWAERCLAEHRRLTEVRSGKPYQALFGVIQGAQYEDLRRRACRDLAGME
IDGHCFDGFGIGGAIEKANLGRIVTWCAEELPEDRPRHLLGISEPDDLFAACRAGADTFDCVSPSRVARNAAIYTVDGRY
NVDTARFRRDFGPLEDDCDCYTCTHYSRAYIHHLFKAKELLANTLATIHNERWTVRLVDQIRGAMCSGDLDAFETEFMGR
WNANGGRLAKVN

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=385, Percent_Identity=35.5844155844156, Blast_Score=248, Evalue=1e-65,
Organism=Escherichia coli, GI1786607, Length=393, Percent_Identity=41.2213740458015, Blast_Score=299, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17544680, Length=385, Percent_Identity=34.2857142857143, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI25146502, Length=217, Percent_Identity=24.4239631336406, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19920510, Length=397, Percent_Identity=35.0125944584383, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI21355311, Length=251, Percent_Identity=27.4900398406374, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 45719; Mature: 45588

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFGFEVTSRLDNTCGRTGLIHTPHGVIQTPAFVVVGTKATVKALLPESVVQLGAQAVLA
CCCCHHHHHHHHHHCCCCCCEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
NAYHLFLQPGPELIDEAGGLGRFMNWTGPTFTDSGGFQVLSLGAGFKKTLAMDVSQLTEA
HHHHEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
DVIAADADRKAMVDDDGVTFRSPLNGDLHRFTPEVSMGIQHHLGADIMFSFDELTTLMNT
HEEEECCCCCCEECCCCCEECCCCCCCHHHCCCHHHHCHHHHCCCHHEEEHHHHHHHHHH
RAYQEEALERTRRWAERCLAEHRRLTEVRSGKPYQALFGVIQGAQYEDLRRRACRDLAGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
EIDGHCFDGFGIGGAIEKANLGRIVTWCAEELPEDRPRHLLGISEPDDLFAACRAGADTF
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHCCCCCC
DCVSPSRVARNAAIYTVDGRYNVDTARFRRDFGPLEDDCDCYTCTHYSRAYIHHLFKAKE
CCCCHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHH
LLANTLATIHNERWTVRLVDQIRGAMCSGDLDAFETEFMGRWNANGGRLAKVN
HHHHHHHHHHCCCEEEEEHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure 
TFGFEVTSRLDNTCGRTGLIHTPHGVIQTPAFVVVGTKATVKALLPESVVQLGAQAVLA
CCCHHHHHHHHHHCCCCCCEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
NAYHLFLQPGPELIDEAGGLGRFMNWTGPTFTDSGGFQVLSLGAGFKKTLAMDVSQLTEA
HHHHEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
DVIAADADRKAMVDDDGVTFRSPLNGDLHRFTPEVSMGIQHHLGADIMFSFDELTTLMNT
HEEEECCCCCCEECCCCCEECCCCCCCHHHCCCHHHHCHHHHCCCHHEEEHHHHHHHHHH
RAYQEEALERTRRWAERCLAEHRRLTEVRSGKPYQALFGVIQGAQYEDLRRRACRDLAGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
EIDGHCFDGFGIGGAIEKANLGRIVTWCAEELPEDRPRHLLGISEPDDLFAACRAGADTF
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHCCCCCC
DCVSPSRVARNAAIYTVDGRYNVDTARFRRDFGPLEDDCDCYTCTHYSRAYIHHLFKAKE
CCCCHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHH
LLANTLATIHNERWTVRLVDQIRGAMCSGDLDAFETEFMGRWNANGGRLAKVN
HHHHHHHHHHCCCEEEEEHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA