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The map label for this gene is rsmE [H]

Identifier: 50842401

GI number: 50842401

Start: 998318

End: 999058

Strand: Direct

Name: rsmE [H]

Synonym: PPA0917

Alternate gene names: 50842401

Gene position: 998318-999058 (Clockwise)

Preceding gene: 50842400

Following gene: 50842408

Centisome position: 38.99

GC content: 61.67

Gene sequence:

>741_bases
ATGAGTGACCCCTGCTTTCTTGGCGAGGTCGTCGCAGCTGTGCCCGGATCGGTGGTAGAAATCACCGGTGCAGAGGGCCA
CCATGCTGGCTCGGTTCGACGCATTCGGGTGGGTGAGTCTGTTCTCGTCACCGACGGGCAGGGTCATGCCGTTCGTGGCC
CTGCCATTGAGGTGACGAAAGGGTCAGTTAGCGTCGAGACCGTTGAGATCCTCCATACTCCCGCGACCACGCATCGATGG
GTCGCCGTCCAGGCATTGCCGAAGTCCGATAGAGCTGAGCTGGCGGTGGCGACCCTCACCGAGATGGGAGTTCACGAAAT
CCTCGCCTGGCAGGCTGATCGGAGCATCGTGCGATGGAAGGGCGACAAGCAAGCCAAGGGCGTCGCGAGGTGGCAAGCGG
CTGCCCGTGAGGCCACCAAACAGTCTCGACGTTTTCTTGTGCCACAGGTAGAACTAGCGCAAACCCGTGAAGTTGTTAAG
CGGATTTGCAATGCCCAGGCCGCCTACGTTTTGCACGAGTCGGCCAGTGAACCGCTGGTGCATCAGGAGCTGCCGGCATC
CGGTGAGGTTATCTTCATCGTCGGGCCAGAGGGTGGCATCACCAGCCAGGAAGTGGAGGCCTTCGTCGCGGCCGGTGCAC
GCCCTGTGACGGTGTCGGACGGGGTATTGAGAACCTCGACCGCCGGTGTGGTCGGCTTAGCCCAGTTGCGGGCTATGGCT
AACCTCACCGCTTGCGATTGA

Upstream 100 bases:

>100_bases
GTCAGCTAGCTGAGGCTCGGGAAGAAACCAATGCTTCGGCGTCGGTAGAGAAATCAGGCGGTCGCGGCATGTTTTCCCGC
ATCAAGGAAGCTTTTGGCGG

Downstream 100 bases:

>100_bases
GAACTTGGTTCGTTGGGTTCAGGGCTCGACGGTCACTTTGATGGCTTCGCCCTTGGCGACGATATCGAAGACCGTCATGA
CGTCGTCGAGTTTGACGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSDPCFLGEVVAAVPGSVVEITGAEGHHAGSVRRIRVGESVLVTDGQGHAVRGPAIEVTKGSVSVETVEILHTPATTHRW
VAVQALPKSDRAELAVATLTEMGVHEILAWQADRSIVRWKGDKQAKGVARWQAAAREATKQSRRFLVPQVELAQTREVVK
RICNAQAAYVLHESASEPLVHQELPASGEVIFIVGPEGGITSQEVEAFVAAGARPVTVSDGVLRTSTAGVVGLAQLRAMA
NLTACD

Sequences:

>Translated_246_residues
MSDPCFLGEVVAAVPGSVVEITGAEGHHAGSVRRIRVGESVLVTDGQGHAVRGPAIEVTKGSVSVETVEILHTPATTHRW
VAVQALPKSDRAELAVATLTEMGVHEILAWQADRSIVRWKGDKQAKGVARWQAAAREATKQSRRFLVPQVELAQTREVVK
RICNAQAAYVLHESASEPLVHQELPASGEVIFIVGPEGGITSQEVEAFVAAGARPVTVSDGVLRTSTAGVVGLAQLRAMA
NLTACD
>Mature_245_residues
SDPCFLGEVVAAVPGSVVEITGAEGHHAGSVRRIRVGESVLVTDGQGHAVRGPAIEVTKGSVSVETVEILHTPATTHRWV
AVQALPKSDRAELAVATLTEMGVHEILAWQADRSIVRWKGDKQAKGVARWQAAAREATKQSRRFLVPQVELAQTREVVKR
ICNAQAAYVLHESASEPLVHQELPASGEVIFIVGPEGGITSQEVEAFVAAGARPVTVSDGVLRTSTAGVVGLAQLRAMAN
LTACD

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=213, Percent_Identity=24.4131455399061, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26014; Mature: 25883

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPCFLGEVVAAVPGSVVEITGAEGHHAGSVRRIRVGESVLVTDGQGHAVRGPAIEVTK
CCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCEEEEECCCCCEEECCEEEEEC
GSVSVETVEILHTPATTHRWVAVQALPKSDRAELAVATLTEMGVHEILAWQADRSIVRWK
CCEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
GDKQAKGVARWQAAAREATKQSRRFLVPQVELAQTREVVKRICNAQAAYVLHESASEPLV
CCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
HQELPASGEVIFIVGPEGGITSQEVEAFVAAGARPVTVSDGVLRTSTAGVVGLAQLRAMA
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEECCCEEEECCCCHHHHHHHHHHH
NLTACD
HCCCCC
>Mature Secondary Structure 
SDPCFLGEVVAAVPGSVVEITGAEGHHAGSVRRIRVGESVLVTDGQGHAVRGPAIEVTK
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEECCEEEEECCCCCEEECCEEEEEC
GSVSVETVEILHTPATTHRWVAVQALPKSDRAELAVATLTEMGVHEILAWQADRSIVRWK
CCEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
GDKQAKGVARWQAAAREATKQSRRFLVPQVELAQTREVVKRICNAQAAYVLHESASEPLV
CCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCH
HQELPASGEVIFIVGPEGGITSQEVEAFVAAGARPVTVSDGVLRTSTAGVVGLAQLRAMA
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEECCCEEEECCCCHHHHHHHHHHH
NLTACD
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]