Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 50842304

GI number: 50842304

Start: 898762

End: 899970

Strand: Direct

Name: pgk

Synonym: PPA0817

Alternate gene names: 50842304

Gene position: 898762-899970 (Clockwise)

Preceding gene: 50842303

Following gene: 50842305

Centisome position: 35.1

GC content: 60.46

Gene sequence:

>1209_bases
ATGAAATCTGTTGAGGATCTCGGAGACTTGCGCGGTAAGCGCGTCGTCATTCGCTGCGACTTCAATGTCCCGCTCGACGG
GGACAAGATCACCGACGACGGCCGTATTAAGGCTGCCCTGCCTACTTTGACCCAGCTGCATGAGGCTGGCGCTCGCACCA
CCGTGCTCGCGCACCTCGGTCGTCCCAAGGGTGCCGTCGATCCCAAGTACTCGCTGGCGCCAGTTGCCAAGCGCCTCGGT
GAGCTTATGAACCTTGACGTCAAGCTTGCTGGGGACGTTGTTGGTCCCTCCGCCACTAAAACCTCTGAGTCGCTGGCTGA
TGGCGAGATCGCCTTGGTCGAAAACGTCCGCTTCGAGAAGGGCGAGACCAGTAAGGACGAGGCCGAGCGCGCTGAGCTCG
CTCAGAAATACGCCTCGTTTGGTGAGTTTTACGTCTCCAACGGCTTCGGCGTTGTGCACCGCAAGCAGGCTTCCGTCTAC
GACGTTGCCAAGCTGCTGCCGAGCGCTGCTGGCTCCCTCGTTGCCAAGGAGGTAGAGGTCCTCGAAGGCCTGACCGCCGA
TCCGAAGCGGCCGTTCGTCGTCGTCCTCGGTGGTGCCAAGGTCGCCGACAAGCTTGCCGTCATCGATAACCTGCTCAAGG
CCGCTGACACTCTCGTTATTGGTGGCGGTATGGCGTACACCTTCCTCAAGGCGAAGGGTCTCGAGGTCGGTGACTCCCTA
CTTGACGAAGATTCGATCGACGCGTGCACGAAGTATCTCGAACAGGCCAAGGCTGCAGGCAAGCAGATCCTGCTTCCCGT
AGACGTGCGAGTGGTCTCCGACATCGATTTCGAGGCCCGCACTGTCGGTCAGATCGACGTCGTGCCCGCTGACGAGATTC
CTTCCGACAAGATGGCAGTAGACATCGGTCCGGAGACCGAGAAGGCTTACGCCAACGCGATCAAGGGTGCCAAGTCAGTG
TTCTGGAACGGACCAATGGGCGTTTTCGAGATCAAGGGCCTCGAGCTCGGCACCAAGGCCGTCGCCCAGGCTCTTACCGA
GGTTGACGGCCTTTCGGTGGTCGGCGGGGGAGACTCCGCCTCCGCCGTGCGTACCCTCGGTTTCGCCGACGATCAGTTCG
GTCATATTTCTACCGGTGGTGGCGCCTCCCTCGAGCTTATGGAGGGCAAGGAGCTACCCGGAATCGCAGTACTCAAGGAG
AACAACTGA

Upstream 100 bases:

>100_bases
CGGCCCCGTGCCCATGCGGGCGCGGGGCCGTCATCTATACCCGGGCCGCGACGCTCGCGAAACATCGTTCACGACTACCA
CTTCAAGAAAGGGCTTCCAC

Downstream 100 bases:

>100_bases
TGTCCCGTACCCCGATCATGGCCGGCAACTGGAAGATGAACCTCGACCACGTTGCCGCTACCTCCCTCGTCCAGGATCTC
GGTGAGGCCCTCAAGGCCGC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKSVEDLGDLRGKRVVIRCDFNVPLDGDKITDDGRIKAALPTLTQLHEAGARTTVLAHLGRPKGAVDPKYSLAPVAKRLG
ELMNLDVKLAGDVVGPSATKTSESLADGEIALVENVRFEKGETSKDEAERAELAQKYASFGEFYVSNGFGVVHRKQASVY
DVAKLLPSAAGSLVAKEVEVLEGLTADPKRPFVVVLGGAKVADKLAVIDNLLKAADTLVIGGGMAYTFLKAKGLEVGDSL
LDEDSIDACTKYLEQAKAAGKQILLPVDVRVVSDIDFEARTVGQIDVVPADEIPSDKMAVDIGPETEKAYANAIKGAKSV
FWNGPMGVFEIKGLELGTKAVAQALTEVDGLSVVGGGDSASAVRTLGFADDQFGHISTGGGASLELMEGKELPGIAVLKE
NN

Sequences:

>Translated_402_residues
MKSVEDLGDLRGKRVVIRCDFNVPLDGDKITDDGRIKAALPTLTQLHEAGARTTVLAHLGRPKGAVDPKYSLAPVAKRLG
ELMNLDVKLAGDVVGPSATKTSESLADGEIALVENVRFEKGETSKDEAERAELAQKYASFGEFYVSNGFGVVHRKQASVY
DVAKLLPSAAGSLVAKEVEVLEGLTADPKRPFVVVLGGAKVADKLAVIDNLLKAADTLVIGGGMAYTFLKAKGLEVGDSL
LDEDSIDACTKYLEQAKAAGKQILLPVDVRVVSDIDFEARTVGQIDVVPADEIPSDKMAVDIGPETEKAYANAIKGAKSV
FWNGPMGVFEIKGLELGTKAVAQALTEVDGLSVVGGGDSASAVRTLGFADDQFGHISTGGGASLELMEGKELPGIAVLKE
NN
>Mature_402_residues
MKSVEDLGDLRGKRVVIRCDFNVPLDGDKITDDGRIKAALPTLTQLHEAGARTTVLAHLGRPKGAVDPKYSLAPVAKRLG
ELMNLDVKLAGDVVGPSATKTSESLADGEIALVENVRFEKGETSKDEAERAELAQKYASFGEFYVSNGFGVVHRKQASVY
DVAKLLPSAAGSLVAKEVEVLEGLTADPKRPFVVVLGGAKVADKLAVIDNLLKAADTLVIGGGMAYTFLKAKGLEVGDSL
LDEDSIDACTKYLEQAKAAGKQILLPVDVRVVSDIDFEARTVGQIDVVPADEIPSDKMAVDIGPETEKAYANAIKGAKSV
FWNGPMGVFEIKGLELGTKAVAQALTEVDGLSVVGGGDSASAVRTLGFADDQFGHISTGGGASLELMEGKELPGIAVLKE
NN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=42.0924574209246, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=41.1192214111922, Blast_Score=280, Evalue=1e-75,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=40.7594936708861, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=41.2776412776413, Blast_Score=289, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6319857, Length=417, Percent_Identity=41.9664268585132, Blast_Score=302, Evalue=6e-83,
Organism=Drosophila melanogaster, GI24581203, Length=412, Percent_Identity=40.5339805825243, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=40.7766990291262, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_PROAC (Q6A9J3)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055531.1
- HSSP:   P09403
- ProteinModelPortal:   Q6A9J3
- SMR:   Q6A9J3
- GeneID:   2933008
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0817
- NMPDR:   fig|267747.1.peg.808
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   PACN267747:PPA0817-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42219; Mature: 42219

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 35-35 BINDING 117-117 BINDING 154-154 BINDING 204-204 BINDING 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVEDLGDLRGKRVVIRCDFNVPLDGDKITDDGRIKAALPTLTQLHEAGARTTVLAHLG
CCCHHHHHHCCCCEEEEEECCCCCCCCCEECCCCEEEEHHHHHHHHHHCCCCEEEHHHCC
RPKGAVDPKYSLAPVAKRLGELMNLDVKLAGDVVGPSATKTSESLADGEIALVENVRFEK
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHCCCCEEEEECCCCCC
GETSKDEAERAELAQKYASFGEFYVSNGFGVVHRKQASVYDVAKLLPSAAGSLVAKEVEV
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LEGLTADPKRPFVVVLGGAKVADKLAVIDNLLKAADTLVIGGGMAYTFLKAKGLEVGDSL
HCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEECCCCEEEEHHHCCCCCCHHH
LDEDSIDACTKYLEQAKAAGKQILLPVDVRVVSDIDFEARTVGQIDVVPADEIPSDKMAV
CCCCCHHHHHHHHHHHHHCCCEEEEEECEEEEECCCCCCEECCEEEEECCCCCCCCCEEE
DIGPETEKAYANAIKGAKSVFWNGPMGVFEIKGLELGTKAVAQALTEVDGLSVVGGGDSA
EECCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCH
SAVRTLGFADDQFGHISTGGGASLELMEGKELPGIAVLKENN
HHHHHCCCCCCCCCEEECCCCCEEEEECCCCCCCEEEEECCC
>Mature Secondary Structure
MKSVEDLGDLRGKRVVIRCDFNVPLDGDKITDDGRIKAALPTLTQLHEAGARTTVLAHLG
CCCHHHHHHCCCCEEEEEECCCCCCCCCEECCCCEEEEHHHHHHHHHHCCCCEEEHHHCC
RPKGAVDPKYSLAPVAKRLGELMNLDVKLAGDVVGPSATKTSESLADGEIALVENVRFEK
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHCCCCEEEEECCCCCC
GETSKDEAERAELAQKYASFGEFYVSNGFGVVHRKQASVYDVAKLLPSAAGSLVAKEVEV
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LEGLTADPKRPFVVVLGGAKVADKLAVIDNLLKAADTLVIGGGMAYTFLKAKGLEVGDSL
HCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCEEEECCCCEEEEHHHCCCCCCHHH
LDEDSIDACTKYLEQAKAAGKQILLPVDVRVVSDIDFEARTVGQIDVVPADEIPSDKMAV
CCCCCHHHHHHHHHHHHHCCCEEEEEECEEEEECCCCCCEECCEEEEECCCCCCCCCEEE
DIGPETEKAYANAIKGAKSVFWNGPMGVFEIKGLELGTKAVAQALTEVDGLSVVGGGDSA
EECCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCH
SAVRTLGFADDQFGHISTGGGASLELMEGKELPGIAVLKENN
HHHHHCCCCCCCCCEEECCCCCEEEEECCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA