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The map label for this gene is tpiA

Identifier: 50842305

GI number: 50842305

Start: 899970

End: 900749

Strand: Direct

Name: tpiA

Synonym: PPA0818

Alternate gene names: 50842305

Gene position: 899970-900749 (Clockwise)

Preceding gene: 50842304

Following gene: 50842306

Centisome position: 35.15

GC content: 60.9

Gene sequence:

>780_bases
ATGTCCCGTACCCCGATCATGGCCGGCAACTGGAAGATGAACCTCGACCACGTTGCCGCTACCTCCCTCGTCCAGGATCT
CGGTGAGGCCCTCAAGGCCGCGGGATACGACTCCTCCAAGTCAGAGGCCGTCGTCATCCCGCCGTTCACTGATATCCGTC
CTGTCGCCATCATCATTGATGGTGACAAGTTGCCGATCGCCTACGGTGCCCAGGATATTTCGGCTCACGACGATGGCGCC
TACACCGGCGAGGTGTCCGGCGCCATGCTGTCGAAGCTGGGTTGCCGCTATGTCGTCGTTGGCCACTCCGAGCGTCGCGA
GTACCACAACGAGTCCGACGAACTGGTCAATGCCAAGGCTAAGAAGGTCATCGAAAATGGAATGACCCCAATCATCTGCT
GTGGTGAGGCCCTCGAGGTTCGCAAGGCTGGCAAGCACGTCGAGCATACCGTTGGCCAGATCAAAGCCGACCTTGACGGC
ATTCCTGCCGAACAGGTCGCCAAGCTTGTCATCGCCTACGAGCCCATCTGGGCCATCGGCACTGGCGAGACTGCCACCGC
TGACGATGCCCAGGAGGTCTGCCAGGCCATCCGCGAGGCCGTCAAGGAGCTTTACGACGCCCCGACCGCCGAGGCGGTGC
GCATTCAGTACGGTGGTTCGGTTAAACCGGCCAACGTCGCTGAGATCATGGCCAAGCCGGACGTCGACGGCGCTCTCGTT
GGCGGAGCCTCGCTCAAAGCTGGCGATTTCTCCAAGATCGTAACTTTCTACGAGGCCTGA

Upstream 100 bases:

>100_bases
ACGATCAGTTCGGTCATATTTCTACCGGTGGTGGCGCCTCCCTCGAGCTTATGGAGGGCAAGGAGCTACCCGGAATCGCA
GTACTCAAGGAGAACAACTG

Downstream 100 bases:

>100_bases
TCAGGCAGTTCCGAGACGGTCCCCGCACCTGATGGTGCGGGGACCGTTCCCGTTCAACCTCTGATTTACGAGCGCGATTC
ACGCATATTTCGAGGATCCA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSRTPIMAGNWKMNLDHVAATSLVQDLGEALKAAGYDSSKSEAVVIPPFTDIRPVAIIIDGDKLPIAYGAQDISAHDDGA
YTGEVSGAMLSKLGCRYVVVGHSERREYHNESDELVNAKAKKVIENGMTPIICCGEALEVRKAGKHVEHTVGQIKADLDG
IPAEQVAKLVIAYEPIWAIGTGETATADDAQEVCQAIREAVKELYDAPTAEAVRIQYGGSVKPANVAEIMAKPDVDGALV
GGASLKAGDFSKIVTFYEA

Sequences:

>Translated_259_residues
MSRTPIMAGNWKMNLDHVAATSLVQDLGEALKAAGYDSSKSEAVVIPPFTDIRPVAIIIDGDKLPIAYGAQDISAHDDGA
YTGEVSGAMLSKLGCRYVVVGHSERREYHNESDELVNAKAKKVIENGMTPIICCGEALEVRKAGKHVEHTVGQIKADLDG
IPAEQVAKLVIAYEPIWAIGTGETATADDAQEVCQAIREAVKELYDAPTAEAVRIQYGGSVKPANVAEIMAKPDVDGALV
GGASLKAGDFSKIVTFYEA
>Mature_258_residues
SRTPIMAGNWKMNLDHVAATSLVQDLGEALKAAGYDSSKSEAVVIPPFTDIRPVAIIIDGDKLPIAYGAQDISAHDDGAY
TGEVSGAMLSKLGCRYVVVGHSERREYHNESDELVNAKAKKVIENGMTPIICCGEALEVRKAGKHVEHTVGQIKADLDGI
PAEQVAKLVIAYEPIWAIGTGETATADDAQEVCQAIREAVKELYDAPTAEAVRIQYGGSVKPANVAEIMAKPDVDGALVG
GASLKAGDFSKIVTFYEA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=245, Percent_Identity=40.8163265306122, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4507645, Length=245, Percent_Identity=40.8163265306122, Blast_Score=158, Evalue=4e-39,
Organism=Escherichia coli, GI1790353, Length=257, Percent_Identity=44.3579766536965, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17536593, Length=256, Percent_Identity=42.96875, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=36.9918699186992, Blast_Score=152, Evalue=7e-38,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=41.1290322580645, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28572008, Length=257, Percent_Identity=40.4669260700389, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28572006, Length=257, Percent_Identity=40.4669260700389, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PROAC (Q6A9J2)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055532.1
- HSSP:   P00943
- ProteinModelPortal:   Q6A9J2
- SMR:   Q6A9J2
- GeneID:   2931451
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0818
- NMPDR:   fig|267747.1.peg.809
- HOGENOM:   HBG708281
- OMA:   HSERANE
- ProtClustDB:   PRK00042
- BioCyc:   PACN267747:PPA0818-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27468; Mature: 27337

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 102-102 ACT_SITE 174-174 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTPIMAGNWKMNLDHVAATSLVQDLGEALKAAGYDSSKSEAVVIPPFTDIRPVAIIID
CCCCCEEECCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEC
GDKLPIAYGAQDISAHDDGAYTGEVSGAMLSKLGCRYVVVGHSERREYHNESDELVNAKA
CCCCCEEECCCCCCCCCCCCEECCHHHHHHHHCCCEEEEECCHHHHHHCCCHHHHHHHHH
KKVIENGMTPIICCGEALEVRKAGKHVEHTVGQIKADLDGIPAEQVAKLVIAYEPIWAIG
HHHHHCCCCEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCEEEEC
TGETATADDAQEVCQAIREAVKELYDAPTAEAVRIQYGGSVKPANVAEIMAKPDVDGALV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCEEE
GGASLKAGDFSKIVTFYEA
CCCCCCCCCHHHHHEEECC
>Mature Secondary Structure 
SRTPIMAGNWKMNLDHVAATSLVQDLGEALKAAGYDSSKSEAVVIPPFTDIRPVAIIID
CCCCEEECCEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEC
GDKLPIAYGAQDISAHDDGAYTGEVSGAMLSKLGCRYVVVGHSERREYHNESDELVNAKA
CCCCCEEECCCCCCCCCCCCEECCHHHHHHHHCCCEEEEECCHHHHHHCCCHHHHHHHHH
KKVIENGMTPIICCGEALEVRKAGKHVEHTVGQIKADLDGIPAEQVAKLVIAYEPIWAIG
HHHHHCCCCEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCEEEEC
TGETATADDAQEVCQAIREAVKELYDAPTAEAVRIQYGGSVKPANVAEIMAKPDVDGALV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCEEE
GGASLKAGDFSKIVTFYEA
CCCCCCCCCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA