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The map label for this gene is gap [H]

Identifier: 50842303

GI number: 50842303

Start: 897632

End: 898639

Strand: Direct

Name: gap [H]

Synonym: PPA0816

Alternate gene names: 50842303

Gene position: 897632-898639 (Clockwise)

Preceding gene: 50842302

Following gene: 50842304

Centisome position: 35.06

GC content: 61.01

Gene sequence:

>1008_bases
ATGACCGTCAAGGTTGGTATCAACGGTTTTGGCCGCATTGGCCGTAACTTCTTCCGCGCCCTTGCTGAGCAGGGCGCCGA
CCTCGAGGTGGTCGCTGTCAACGACCTCACCGACACCAAGACGCTGGCCCACCTCCTGAAGTACGACTCGATCATGGGTC
GTTTCTCCGGTGAGGTCAGCTACGACGATGACTCCATCACCGTTGACGGCAAGACCATCAAGGTTCTCGCCGAGCGCAAC
CCCGCTGACCTGCCCTGGAAGGAGCTCGGCGCCGAAATCGTCGTCGAGTCCACTGGACTCTTCACTGATGGCGAGAAGGC
CAAGGCCCACTTTGAGGCCGGCGCCAAGAAGGTCATCATCTCCGCTCCCGGCAAGAACGTCGACGGCACCTTCGTCATGG
GCGTCAACGATGGCGACTACGACAACGCGAAGCACAACATCATCTCGAACGCTTCTTGCACCACCAACTGCCTCGCTCCG
CTTGCCAAAGTCCTCAATGACGCCTTCGGTATCGAGCGCGGCATCATGACTACCGTCCACGCCTACACCGGTGATCAGCG
TCTGCAGGATGCTCCTCACAAGGATCTGCGTCGTGCCCGCGCCGCTGCCCTCAACATGATCCCCACCAAGACCGGTGCCG
CTCAGGCTGTGGCCCTGGTCCTCCCGGAGCTCAAGGGCAAGTTCGACGGCCTTGCCGTCCGCGTTCCGACCCCGACCGGC
TCTCTGACTGACCTGACCTTCCAGACCTCTAAGGAGACCACCGTTGAGGAGGTTCAGGCCGCCGTTAAGAAGGCTGCCGA
GGGTCCGCTCAAGGGCATCCTGGCCTACACCGAGGACCCGATCGTGTCGAAGGACATCGAGGGCGACCCGCACTCCTCGA
TCTTCGATGCTACCGAGACCAAGGTCATCGGAAACCTTGTTAAGGTCCTCTCCTGGTACGACAACGAGTGGGGCTACTCC
AACCGTCTGGTTGACCTCACCAAGCTCGTCGCCAGCAAGCTTGCCTGA

Upstream 100 bases:

>100_bases
CTTAGTAATGGGCCCTAGGGTTTGTCCATTGCCCTTAGTCGCTATGCTTAGAGATGTCCGAGTGCTGAACGCACAAACCT
CATTTGGAGGAGATCCCTTA

Downstream 100 bases:

>100_bases
TCTCACCATCGTGAGCTGATGACGGCCCCGTGCCCATGCGGGCGCGGGGCCGTCATCTATACCCGGGCCGCGACGCTCGC
GAAACATCGTTCACGACTAC

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTVKVGINGFGRIGRNFFRALAEQGADLEVVAVNDLTDTKTLAHLLKYDSIMGRFSGEVSYDDDSITVDGKTIKVLAERN
PADLPWKELGAEIVVESTGLFTDGEKAKAHFEAGAKKVIISAPGKNVDGTFVMGVNDGDYDNAKHNIISNASCTTNCLAP
LAKVLNDAFGIERGIMTTVHAYTGDQRLQDAPHKDLRRARAAALNMIPTKTGAAQAVALVLPELKGKFDGLAVRVPTPTG
SLTDLTFQTSKETTVEEVQAAVKKAAEGPLKGILAYTEDPIVSKDIEGDPHSSIFDATETKVIGNLVKVLSWYDNEWGYS
NRLVDLTKLVASKLA

Sequences:

>Translated_335_residues
MTVKVGINGFGRIGRNFFRALAEQGADLEVVAVNDLTDTKTLAHLLKYDSIMGRFSGEVSYDDDSITVDGKTIKVLAERN
PADLPWKELGAEIVVESTGLFTDGEKAKAHFEAGAKKVIISAPGKNVDGTFVMGVNDGDYDNAKHNIISNASCTTNCLAP
LAKVLNDAFGIERGIMTTVHAYTGDQRLQDAPHKDLRRARAAALNMIPTKTGAAQAVALVLPELKGKFDGLAVRVPTPTG
SLTDLTFQTSKETTVEEVQAAVKKAAEGPLKGILAYTEDPIVSKDIEGDPHSSIFDATETKVIGNLVKVLSWYDNEWGYS
NRLVDLTKLVASKLA
>Mature_334_residues
TVKVGINGFGRIGRNFFRALAEQGADLEVVAVNDLTDTKTLAHLLKYDSIMGRFSGEVSYDDDSITVDGKTIKVLAERNP
ADLPWKELGAEIVVESTGLFTDGEKAKAHFEAGAKKVIISAPGKNVDGTFVMGVNDGDYDNAKHNIISNASCTTNCLAPL
AKVLNDAFGIERGIMTTVHAYTGDQRLQDAPHKDLRRARAAALNMIPTKTGAAQAVALVLPELKGKFDGLAVRVPTPTGS
LTDLTFQTSKETTVEEVQAAVKKAAEGPLKGILAYTEDPIVSKDIEGDPHSSIFDATETKVIGNLVKVLSWYDNEWGYSN
RLVDLTKLVASKLA

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7657116, Length=332, Percent_Identity=50.9036144578313, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI7669492, Length=334, Percent_Identity=51.7964071856287, Blast_Score=328, Evalue=4e-90,
Organism=Escherichia coli, GI1788079, Length=328, Percent_Identity=55.4878048780488, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1789295, Length=336, Percent_Identity=48.8095238095238, Blast_Score=328, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17534677, Length=337, Percent_Identity=53.4124629080119, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17534679, Length=337, Percent_Identity=53.4124629080119, Blast_Score=338, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI32566163, Length=337, Percent_Identity=53.4124629080119, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=53.4124629080119, Blast_Score=332, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6322409, Length=332, Percent_Identity=54.2168674698795, Blast_Score=348, Evalue=5e-97,
Organism=Saccharomyces cerevisiae, GI6321631, Length=332, Percent_Identity=54.8192771084337, Blast_Score=348, Evalue=6e-97,
Organism=Saccharomyces cerevisiae, GI6322468, Length=332, Percent_Identity=54.8192771084337, Blast_Score=348, Evalue=7e-97,
Organism=Drosophila melanogaster, GI85725000, Length=333, Percent_Identity=52.2522522522522, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI22023983, Length=333, Percent_Identity=52.2522522522522, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI17933600, Length=333, Percent_Identity=51.9519519519519, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI18110149, Length=333, Percent_Identity=51.9519519519519, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=47.5460122699387, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35978; Mature: 35847

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00071 GAPDH ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKVGINGFGRIGRNFFRALAEQGADLEVVAVNDLTDTKTLAHLLKYDSIMGRFSGEVS
CEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEE
YDDDSITVDGKTIKVLAERNPADLPWKELGAEIVVESTGLFTDGEKAKAHFEAGAKKVII
ECCCCEEECCCEEEEEECCCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEE
SAPGKNVDGTFVMGVNDGDYDNAKHNIISNASCTTNCLAPLAKVLNDAFGIERGIMTTVH
ECCCCCCCCEEEEECCCCCCCCCHHCEECCCCCHHHHHHHHHHHHHHHHHHHHCHHEEEE
AYTGDQRLQDAPHKDLRRARAAALNMIPTKTGAAQAVALVLPELKGKFDGLAVRVPTPTG
ECCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
SLTDLTFQTSKETTVEEVQAAVKKAAEGPLKGILAYTEDPIVSKDIEGDPHSSIFDATET
CCEEEEEECCCCHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCCHHHHHHH
KVIGNLVKVLSWYDNEWGYSNRLVDLTKLVASKLA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVKVGINGFGRIGRNFFRALAEQGADLEVVAVNDLTDTKTLAHLLKYDSIMGRFSGEVS
EEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEE
YDDDSITVDGKTIKVLAERNPADLPWKELGAEIVVESTGLFTDGEKAKAHFEAGAKKVII
ECCCCEEECCCEEEEEECCCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEE
SAPGKNVDGTFVMGVNDGDYDNAKHNIISNASCTTNCLAPLAKVLNDAFGIERGIMTTVH
ECCCCCCCCEEEEECCCCCCCCCHHCEECCCCCHHHHHHHHHHHHHHHHHHHHCHHEEEE
AYTGDQRLQDAPHKDLRRARAAALNMIPTKTGAAQAVALVLPELKGKFDGLAVRVPTPTG
ECCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCC
SLTDLTFQTSKETTVEEVQAAVKKAAEGPLKGILAYTEDPIVSKDIEGDPHSSIFDATET
CCEEEEEECCCCHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCCHHHHHHH
KVIGNLVKVLSWYDNEWGYSNRLVDLTKLVASKLA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400158; 12948626 [H]