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The map label for this gene is tcyP [H]

Identifier: 50365467

GI number: 50365467

Start: 757701

End: 759458

Strand: Reverse

Name: tcyP [H]

Synonym: Mfl652

Alternate gene names: 50365467

Gene position: 759458-757701 (Counterclockwise)

Preceding gene: 50365468

Following gene: 50365466

Centisome position: 95.74

GC content: 30.94

Gene sequence:

>1758_bases
ATGTTATTAAGCAGTTTTAAAGAAACATTCCTTCAAAACTTTTTAAGCATAAGCACATGACAATCACTTTTAGCGATTGT
ATTGTTTTTTGGTTTAATGGGTGGGTTTTGATATGGATTAAGACAAATAAAAATTAAATTTGTATACAGAATATTTATAG
GTATATCTATAGGTTTAATATTTGGTATAGCAATTCAAGCAATTATAGGTTTCCCTGGAGGTTCATGATTTTCACATGAT
GGACAATCAGCTTGGGCGCTTGTTGATGGCAATTGATTAAATATTTCAGAATATTATATTGAAAAGCCAAATCTTGAAGG
AATGGCTGAAATAAAAGGTAACTATGTTCAAATAAAATTAATAATTGGATTAGACGGATACGAAGCGATAATAAACGGAT
TAACAAAACTTTCTTTATTTAAAGATGCTAAAGGAGCAAATGTAGCTTTTAATTCAGCAATTTCTTTTTCACCAGAAGTA
AAGTCTGGAATAAATTGAATAATTGAATTTAATACGTGAGCATCAATGTTAAGACAAATATTTATTAATGGTATTTTATT
AATTACAATACCAGTTGTATTTATTGCAATCTTTAGAGTTGTTGCTAAACCTGGAAGTAAAGGTTTAGGAAGAATATCTG
GAAAAGCTGTAGCTATTTTACTAATAAATGTAGCTATTGCATTTACAATTACTTTCTGAATAGGATACGTTTTAAAAATT
GGTAAAGGATTAAATTTTGATCAACAAATACCTGGTGGTTCTGGAAGAACAGATTCTAAAGCTTTACCAGAAATAATTTG
GGGATACATACCTTCTAATTTCGTTGCAACATTATCAGCAACAGCAATCATACCCGTTATTGTGCTTGCTGCATTGGTTG
GATATGCAACAACATTCGTAAGAAAAAAACATCCTGAGTCAATGGATAATTTAATGAATTTCATGGACAGAGCTTGAGAT
ATAGTTATGTCTATATTAATGACATTTATGAAAATAATGCCATTAGCAGTAATGGCCATGTTGACAACTTCAATTACAAC
AAGAGCTATAGGTGCACTTGCTTCTATAGGTTTAATATTAGGAGTTGGATATTTAGGTTTAATAATTATGTTAGGTGTTA
TGACTTTAATTTTATTCTTATCAGGAATTAATGTTAAAGCTTGATGAAAACATGCTTGAAGACCTTTTGTTCAAGCTTTC
TCAACACAGTCTTCAAATGCAACACTGCCAGTAACTATGGACACATTGTCTAATGGCATGAAAATTAAAGATAAGGTAAC
AAGCATAGTAGCTCCACTGTCTACTTCACTTGGGCTTGTTGCTTGTGCTGGGGTTCAAGCAGGATTAATTACTTCTGTTT
TATACACAGGTTCTGAAACTGTTGCATCAATGAACATATTTGCATTCTTTATATTAGGACTATTTACTACAATTGTTGCA
AGTATTGGTATTGCTGGTGTTCCCGGTACTGCAACTGTTGTAACATCTGCAGTTTTAAATGGAATTGGTTTTGGAGCTTT
CTTTGCTCCTGTTTATGCAATTTTTGGTGCTATTGATGGTTTATTTGACATGGGTAGAACAGCAACAAATGTTACTGGTG
GAATTTTTGCCGCTACGCTTGTTGCAAAAGATGAATTATTATTTGAAGAAGGTACAGAGTTAATATCAGAAAAAAAATTA
ACAGCATTAATTGAAAATAAAGAAAAAAAAGAAAACTTAAAAAAAGCGTCAAAAGATAAAAAGAAAACAAACAAATAA

Upstream 100 bases:

>100_bases
TTTGCCATACTCTCCACCGTTTTCAAGAACAACAGATATTATTCATATTGCTTTAAGTAAGTTAATATAAATTTAAGAAA
AGAAAAAAGAGGATATAAAA

Downstream 100 bases:

>100_bases
AAAAATTGATAAAATATATAAAGTTTGAAAATTACAAAGGAGAATACATGACTAAGTTCAGATTAAGATATGCACCATCG
CCAACTGGTTTCTTACACAT

Product: putative sodium-glutamate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Symporter yhcL; Transporter of cystine tcyP [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MLLSSFKETFLQNFLSISTWQSLLAIVLFFGLMGGFWYGLRQIKIKFVYRIFIGISIGLIFGIAIQAIIGFPGGSWFSHD
GQSAWALVDGNWLNISEYYIEKPNLEGMAEIKGNYVQIKLIIGLDGYEAIINGLTKLSLFKDAKGANVAFNSAISFSPEV
KSGINWIIEFNTWASMLRQIFINGILLITIPVVFIAIFRVVAKPGSKGLGRISGKAVAILLINVAIAFTITFWIGYVLKI
GKGLNFDQQIPGGSGRTDSKALPEIIWGYIPSNFVATLSATAIIPVIVLAALVGYATTFVRKKHPESMDNLMNFMDRAWD
IVMSILMTFMKIMPLAVMAMLTTSITTRAIGALASIGLILGVGYLGLIIMLGVMTLILFLSGINVKAWWKHAWRPFVQAF
STQSSNATLPVTMDTLSNGMKIKDKVTSIVAPLSTSLGLVACAGVQAGLITSVLYTGSETVASMNIFAFFILGLFTTIVA
SIGIAGVPGTATVVTSAVLNGIGFGAFFAPVYAIFGAIDGLFDMGRTATNVTGGIFAATLVAKDELLFEEGTELISEKKL
TALIENKEKKENLKKASKDKKKTNK

Sequences:

>Translated_585_residues
MLLSSFKETFLQNFLSIST*QSLLAIVLFFGLMGGF*YGLRQIKIKFVYRIFIGISIGLIFGIAIQAIIGFPGGS*FSHD
GQSAWALVDGN*LNISEYYIEKPNLEGMAEIKGNYVQIKLIIGLDGYEAIINGLTKLSLFKDAKGANVAFNSAISFSPEV
KSGIN*IIEFNT*ASMLRQIFINGILLITIPVVFIAIFRVVAKPGSKGLGRISGKAVAILLINVAIAFTITF*IGYVLKI
GKGLNFDQQIPGGSGRTDSKALPEIIWGYIPSNFVATLSATAIIPVIVLAALVGYATTFVRKKHPESMDNLMNFMDRA*D
IVMSILMTFMKIMPLAVMAMLTTSITTRAIGALASIGLILGVGYLGLIIMLGVMTLILFLSGINVKA**KHA*RPFVQAF
STQSSNATLPVTMDTLSNGMKIKDKVTSIVAPLSTSLGLVACAGVQAGLITSVLYTGSETVASMNIFAFFILGLFTTIVA
SIGIAGVPGTATVVTSAVLNGIGFGAFFAPVYAIFGAIDGLFDMGRTATNVTGGIFAATLVAKDELLFEEGTELISEKKL
TALIENKEKKENLKKASKDKKKTNK
>Mature_585_residues
MLLSSFKETFLQNFLSIST*QSLLAIVLFFGLMGGF*YGLRQIKIKFVYRIFIGISIGLIFGIAIQAIIGFPGGS*FSHD
GQSAWALVDGN*LNISEYYIEKPNLEGMAEIKGNYVQIKLIIGLDGYEAIINGLTKLSLFKDAKGANVAFNSAISFSPEV
KSGIN*IIEFNT*ASMLRQIFINGILLITIPVVFIAIFRVVAKPGSKGLGRISGKAVAILLINVAIAFTITF*IGYVLKI
GKGLNFDQQIPGGSGRTDSKALPEIIWGYIPSNFVATLSATAIIPVIVLAALVGYATTFVRKKHPESMDNLMNFMDRA*D
IVMSILMTFMKIMPLAVMAMLTTSITTRAIGALASIGLILGVGYLGLIIMLGVMTLILFLSGINVKA**KHA*RPFVQAF
STQSSNATLPVTMDTLSNGMKIKDKVTSIVAPLSTSLGLVACAGVQAGLITSVLYTGSETVASMNIFAFFILGLFTTIVA
SIGIAGVPGTATVVTSAVLNGIGFGAFFAPVYAIFGAIDGLFDMGRTATNVTGGIFAATLVAKDELLFEEGTELISEKKL
TALIENKEKKENLKKASKDKKKTNK

Specific function: Mediates uptake of L-cystine, the oxidized form of L- cysteine. Although it is more specific for L-cystine, it could also transport a much broader range of amino acids and sulfur compounds including S-methylcysteine [H]

COG id: COG1823

COG function: function code R; Predicted Na+/dicarboxylate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1790514, Length=404, Percent_Identity=26.2376237623762, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788024, Length=383, Percent_Identity=24.5430809399478, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 60960; Mature: 60960

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00267 TACHYKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSSFKETFLQNFLSISTQSLLAIVLFFGLMGGFYGLRQIKIKFVYRIFIGISIGLIFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAIQAIIGFPGGSFSHDGQSAWALVDGNLNISEYYIEKPNLEGMAEIKGNYVQIKLIIGL
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHEEEECCCCCCHHHCCCCEEEEEEEEEC
DGYEAIINGLTKLSLFKDAKGANVAFNSAISFSPEVKSGINIIEFNTASMLRQIFINGIL
CHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHCCCCEEEECHHHHHHHHHHCCHH
LITIPVVFIAIFRVVAKPGSKGLGRISGKAVAILLINVAIAFTITFIGYVLKIGKGLNFD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QQIPGGSGRTDSKALPEIIWGYIPSNFVATLSATAIIPVIVLAALVGYATTFVRKKHPES
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
MDNLMNFMDRADIVMSILMTFMKIMPLAVMAMLTTSITTRAIGALASIGLILGVGYLGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMLGVMTLILFLSGINVKAKHARPFVQAFSTQSSNATLPVTMDTLSNGMKIKDKVTSIVA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHH
PLSTSLGLVACAGVQAGLITSVLYTGSETVASMNIFAFFILGLFTTIVASIGIAGVPGTA
HHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
TVVTSAVLNGIGFGAFFAPVYAIFGAIDGLFDMGRTATNVTGGIFAATLVAKDELLFEEG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHH
TELISEKKLTALIENKEKKENLKKASKDKKKTNK
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLSSFKETFLQNFLSISTQSLLAIVLFFGLMGGFYGLRQIKIKFVYRIFIGISIGLIFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAIQAIIGFPGGSFSHDGQSAWALVDGNLNISEYYIEKPNLEGMAEIKGNYVQIKLIIGL
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHEEEECCCCCCHHHCCCCEEEEEEEEEC
DGYEAIINGLTKLSLFKDAKGANVAFNSAISFSPEVKSGINIIEFNTASMLRQIFINGIL
CHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHCCCCEEEECHHHHHHHHHHCCHH
LITIPVVFIAIFRVVAKPGSKGLGRISGKAVAILLINVAIAFTITFIGYVLKIGKGLNFD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QQIPGGSGRTDSKALPEIIWGYIPSNFVATLSATAIIPVIVLAALVGYATTFVRKKHPES
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
MDNLMNFMDRADIVMSILMTFMKIMPLAVMAMLTTSITTRAIGALASIGLILGVGYLGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMLGVMTLILFLSGINVKAKHARPFVQAFSTQSSNATLPVTMDTLSNGMKIKDKVTSIVA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHH
PLSTSLGLVACAGVQAGLITSVLYTGSETVASMNIFAFFILGLFTTIVASIGIAGVPGTA
HHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
TVVTSAVLNGIGFGAFFAPVYAIFGAIDGLFDMGRTATNVTGGIFAATLVAKDELLFEEG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHH
TELISEKKLTALIENKEKKENLKKASKDKKKTNK
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]