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The map label for this gene is glyA

Identifier: 50364921

GI number: 50364921

Start: 131651

End: 132889

Strand: Direct

Name: glyA

Synonym: Mfl106

Alternate gene names: 50364921

Gene position: 131651-132889 (Clockwise)

Preceding gene: 50364920

Following gene: 50364922

Centisome position: 16.6

GC content: 30.19

Gene sequence:

>1239_bases
ATGAGTACAATAAATAAAAATATTTTAGAATCACTTAAAGGTGAATTAAAAAGACAACAAGATCACATTGAATTAATAGC
AAGTGAAAACTATGTTAGTGATGCAGTTTTACAATTATCAGGAAGTATATTAACTAATAAATATGCAGAAGGTTACCCAG
ATAAAAGGTATTATGGTGGATGTGAATTTGTTGATCAAATTGAAAAACAAGGAATTGAATTAGCTAAAAAAATATTTAAT
GCAGGTCACGCTAATTTACAACCTCATTCAGGTAGTCAAGCTAATGAAGCTGTTTATCGTGCTTTATTACAAAACGGAGA
TAAAGTTGTTTCTATGAGTTTAGACGCTGGAGGTCACTTAACACATGGTTATCCAATTAATTTTTCAGGTAATAATTATG
ATTTTAAATTTTATGGAGTAAATAGAGAAACAGAAGAAATTGATTTTGATGAAGTTAGAAAAGTTGTTTTAGAACATCAA
CCAAAATTAATTGTCGCTGGAGCTAGTGCTTACTCAAGAATTATAGACTTTAAAAAGTTTAGAGAAATTGCAGATGAAGT
TGGAGCATTATTAATGGTTGATATGGCTCATATTGCTGGTTTAGTTGCTGGTGGTGCTCACCCAAACCCTATGGAATATG
CAGATGTTGTTACAACAACAACACACAAAACTTTAAGAGGCGCAAGAGGTGGAATGATTTTATCAAAAGCTGAAATTGGT
AAAAAAATTGATTCATCAGTTTTCCCAGGTACTCAAGGTGGACCTTTAGAAAATCAAATAGCTGGGAAAGTTCAAGCCTT
ATATGAAGCAGATACTCCTGAATTTAAAGAATATGTTCATCAAGTAGTTGCTAACTCAAAAGCATTTGCTAAAGCCTTAG
CAGATAATGGAATGAGATTAATAGCTAATGGAACAGATAATCATTTAATTAATTTAGATGTAAAAAATACTTTAAATGTT
ACTGGAAAAGATGCAGAAAAAATCCTTGAAAGTATTGGTATAGTTTCAAATAAAAACATGATTCCTTTTGATACAGAAAA
ACCTTTTGTTACAAGTGGAATTAGAGTTGGAACTGCAGCAATGACAACTAGAGGATTTAAAGAAGAACAATTTGTTGAAG
TTGCTAAAATAATTGCAAGTGCATTAAAAGATCAATCAGAAACAAATTTAAATACTCTTTCAAAAGAAGTTGCTAAATTA
TGTAAGCAATTCCCAATATATGAACATTTATCATATTAA

Upstream 100 bases:

>100_bases
GCTAGATTAATTGCTGTTGATAAAGCACTTTTATTAGTAGACACTTTCTTAAATACTGAATTTGAAGGTGGAAGACATCA
AGAAAGAGTAGGAACATTAG

Downstream 100 bases:

>100_bases
TACTTTATAATATTAATAATTTGGACAAATAGAGGGAAATAAGTTATGGCATTTACAGAATTGAAACATCCATTGATAAT
TGATAAATTATCAAGAATGC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSTINKNILESLKGELKRQQDHIELIASENYVSDAVLQLSGSILTNKYAEGYPDKRYYGGCEFVDQIEKQGIELAKKIFN
AGHANLQPHSGSQANEAVYRALLQNGDKVVSMSLDAGGHLTHGYPINFSGNNYDFKFYGVNRETEEIDFDEVRKVVLEHQ
PKLIVAGASAYSRIIDFKKFREIADEVGALLMVDMAHIAGLVAGGAHPNPMEYADVVTTTTHKTLRGARGGMILSKAEIG
KKIDSSVFPGTQGGPLENQIAGKVQALYEADTPEFKEYVHQVVANSKAFAKALADNGMRLIANGTDNHLINLDVKNTLNV
TGKDAEKILESIGIVSNKNMIPFDTEKPFVTSGIRVGTAAMTTRGFKEEQFVEVAKIIASALKDQSETNLNTLSKEVAKL
CKQFPIYEHLSY

Sequences:

>Translated_412_residues
MSTINKNILESLKGELKRQQDHIELIASENYVSDAVLQLSGSILTNKYAEGYPDKRYYGGCEFVDQIEKQGIELAKKIFN
AGHANLQPHSGSQANEAVYRALLQNGDKVVSMSLDAGGHLTHGYPINFSGNNYDFKFYGVNRETEEIDFDEVRKVVLEHQ
PKLIVAGASAYSRIIDFKKFREIADEVGALLMVDMAHIAGLVAGGAHPNPMEYADVVTTTTHKTLRGARGGMILSKAEIG
KKIDSSVFPGTQGGPLENQIAGKVQALYEADTPEFKEYVHQVVANSKAFAKALADNGMRLIANGTDNHLINLDVKNTLNV
TGKDAEKILESIGIVSNKNMIPFDTEKPFVTSGIRVGTAAMTTRGFKEEQFVEVAKIIASALKDQSETNLNTLSKEVAKL
CKQFPIYEHLSY
>Mature_411_residues
STINKNILESLKGELKRQQDHIELIASENYVSDAVLQLSGSILTNKYAEGYPDKRYYGGCEFVDQIEKQGIELAKKIFNA
GHANLQPHSGSQANEAVYRALLQNGDKVVSMSLDAGGHLTHGYPINFSGNNYDFKFYGVNRETEEIDFDEVRKVVLEHQP
KLIVAGASAYSRIIDFKKFREIADEVGALLMVDMAHIAGLVAGGAHPNPMEYADVVTTTTHKTLRGARGGMILSKAEIGK
KIDSSVFPGTQGGPLENQIAGKVQALYEADTPEFKEYVHQVVANSKAFAKALADNGMRLIANGTDNHLINLDVKNTLNVT
GKDAEKILESIGIVSNKNMIPFDTEKPFVTSGIRVGTAAMTTRGFKEEQFVEVAKIIASALKDQSETNLNTLSKEVAKLC
KQFPIYEHLSY

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=452, Percent_Identity=40.7079646017699, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI261862352, Length=452, Percent_Identity=40.7079646017699, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI261862350, Length=452, Percent_Identity=40.7079646017699, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI261862348, Length=452, Percent_Identity=40.7079646017699, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI261862346, Length=452, Percent_Identity=40.2654867256637, Blast_Score=304, Evalue=8e-83,
Organism=Homo sapiens, GI22547186, Length=413, Percent_Identity=44.5520581113801, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI22547189, Length=398, Percent_Identity=42.713567839196, Blast_Score=275, Evalue=6e-74,
Organism=Escherichia coli, GI1788902, Length=411, Percent_Identity=52.0681265206813, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25144732, Length=424, Percent_Identity=40.3301886792453, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25144729, Length=424, Percent_Identity=40.3301886792453, Blast_Score=305, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6319739, Length=432, Percent_Identity=44.212962962963, Blast_Score=338, Evalue=9e-94,
Organism=Saccharomyces cerevisiae, GI6323087, Length=442, Percent_Identity=41.6289592760181, Blast_Score=322, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24640005, Length=402, Percent_Identity=44.0298507462687, Blast_Score=333, Evalue=9e-92,
Organism=Drosophila melanogaster, GI221329721, Length=402, Percent_Identity=44.0298507462687, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_MESFL (Q6F211)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053346.1
- ProteinModelPortal:   Q6F211
- SMR:   Q6F211
- GeneID:   2898166
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl106
- HOGENOM:   HBG301263
- OMA:   ADEVGSW
- ProtClustDB:   PRK00011
- BioCyc:   MFLO265311:MFL106-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45190; Mature: 45059

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTINKNILESLKGELKRQQDHIELIASENYVSDAVLQLSGSILTNKYAEGYPDKRYYGG
CCCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
CEFVDQIEKQGIELAKKIFNAGHANLQPHSGSQANEAVYRALLQNGDKVVSMSLDAGGHL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCC
THGYPINFSGNNYDFKFYGVNRETEEIDFDEVRKVVLEHQPKLIVAGASAYSRIIDFKKF
CCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHH
REIADEVGALLMVDMAHIAGLVAGGAHPNPMEYADVVTTTTHKTLRGARGGMILSKAEIG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHH
KKIDSSVFPGTQGGPLENQIAGKVQALYEADTPEFKEYVHQVVANSKAFAKALADNGMRL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCEE
IANGTDNHLINLDVKNTLNVTGKDAEKILESIGIVSNKNMIPFDTEKPFVTSGIRVGTAA
EECCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECHHH
MTTRGFKEEQFVEVAKIIASALKDQSETNLNTLSKEVAKLCKQFPIYEHLSY
HHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
STINKNILESLKGELKRQQDHIELIASENYVSDAVLQLSGSILTNKYAEGYPDKRYYGG
CCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCC
CEFVDQIEKQGIELAKKIFNAGHANLQPHSGSQANEAVYRALLQNGDKVVSMSLDAGGHL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCC
THGYPINFSGNNYDFKFYGVNRETEEIDFDEVRKVVLEHQPKLIVAGASAYSRIIDFKKF
CCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHH
REIADEVGALLMVDMAHIAGLVAGGAHPNPMEYADVVTTTTHKTLRGARGGMILSKAEIG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHH
KKIDSSVFPGTQGGPLENQIAGKVQALYEADTPEFKEYVHQVVANSKAFAKALADNGMRL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCEE
IANGTDNHLINLDVKNTLNVTGKDAEKILESIGIVSNKNMIPFDTEKPFVTSGIRVGTAA
EECCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECHHH
MTTRGFKEEQFVEVAKIIASALKDQSETNLNTLSKEVAKLCKQFPIYEHLSY
HHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA