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The map label for this gene is purB [H]

Identifier: 50364890

GI number: 50364890

Start: 96737

End: 98035

Strand: Direct

Name: purB [H]

Synonym: Mfl075

Alternate gene names: 50364890

Gene position: 96737-98035 (Clockwise)

Preceding gene: 50364889

Following gene: 50364891

Centisome position: 12.2

GC content: 25.17

Gene sequence:

>1299_bases
ATGATAAAAAGATATTCTGTAAATAAGATAGAAAAGATTTGAGACGAAAACAATAAATTAAATGTTTGACTTGATGTAGA
GAAAAAAGTAACTTTTGCATGAATGAAATTAAATGTTATTTCAAAAGAAGAATATTTGCTAATTGATAAAAATGCAAAAA
TAAACATTGGTAGAATGCTAGAAATAGAAAATGAAACACGTCATGATGTTGTTGCGTTTACTAGAGCTATTTCTGAAACA
CTAGGTAATGAAAAAAAATGAATTCATCTTGGTCTGACTTCAACTGATGTTGTTGATACAGCACAAAACAAATTAATACA
AGAATCTAATGAAGTTGTTTTAGAGTCATTAATAAACTTAAAAAATACATTAAAAGAGAAAGCATTAATCTATAAAAAAA
CTTTAACAATGGGAAGATCTCATGGTATTTATGGTGAACCAACTTCTTTAGGACTTAAATTTTTATTATGATTTGATGAA
ATAGACAGACAAATTGAAAGATTTTCATTAGCCAGAAAACAAATTGAAGTAGCGAAAATATCTGGCTCAATGGGAAACTA
CGCTAATTTAGAATTTGAAATAGAAGAAATTGTTGCAGAACAAATGGGTTTAGGGATTGATAATATATCAACTCAAGTAA
CTCAACGTGATAGACATGCATTTTTGATAAGTGTATTTGCCAACATTGCATCTACTCTTGAAAAAATTGCTACTGAAATA
AGATTATTTCAAAGAAGTGAAGTTAAAGAATGGCATGAAGGTTTTAAAGCAAATCAAAAAGGATCAAGCTCAATGCCTCA
TAAAAAAAATCCAATAAGTTCTGAAAATATAACTGGTCTTTCAAGATACTTAAGATCATTTACTAATACAGCTTTTGAAA
ATAATGTGTTATGACATGAAAGAGATATTTCACATAGTTCTAATGAAAGAATTATTTTTAGTGATGTATTCAATGTTTTA
GTTTACACATTAGATAGATTAAAAAATACATTAGACGATTTAGTAATAGATGAAAATAAAATGTTAGAACACATTAATTC
TCAGTTTAATGTTTTTTACAGTCAACCAATATTAAATTACCTTCTTATTAATTGCGATAAATCAAGAGAAGAAATATATG
ATTTTATTCAGAAATGTACTTTTGAAACAATGAATAATAAAGTAGACTTTAAACAATCTTTAATTAATAACGGTATAGAA
AAATATATTGATAATATAGAAGTATTAAATAAACTTTTTAATATTGATTACTTCATAAGAAATGTTGAGAAGATATATAA
CAGAACATTAAATAAATAA

Upstream 100 bases:

>100_bases
CCGAAATTTGTGAAGTTGAAATATCTGGATTTTCAGTAGGTCCTGATAGATTACAAACTGTTATAACTAAAGAAATAATG
TAGAAAATTTAAGGTAAAAT

Downstream 100 bases:

>100_bases
TTTGATATTATAAAAAAAAGGGGAAAAATTAAAAATGCTTTCAAAAAAAGAAAATGTACATATATTTGGTTTAACTCAAG
GGGTTGAACTAGCACAAGAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIKRYSVNKIEKIWDENNKLNVWLDVEKKVTFAWMKLNVISKEEYLLIDKNAKINIGRMLEIENETRHDVVAFTRAISET
LGNEKKWIHLGLTSTDVVDTAQNKLIQESNEVVLESLINLKNTLKEKALIYKKTLTMGRSHGIYGEPTSLGLKFLLWFDE
IDRQIERFSLARKQIEVAKISGSMGNYANLEFEIEEIVAEQMGLGIDNISTQVTQRDRHAFLISVFANIASTLEKIATEI
RLFQRSEVKEWHEGFKANQKGSSSMPHKKNPISSENITGLSRYLRSFTNTAFENNVLWHERDISHSSNERIIFSDVFNVL
VYTLDRLKNTLDDLVIDENKMLEHINSQFNVFYSQPILNYLLINCDKSREEIYDFIQKCTFETMNNKVDFKQSLINNGIE
KYIDNIEVLNKLFNIDYFIRNVEKIYNRTLNK

Sequences:

>Translated_432_residues
MIKRYSVNKIEKI*DENNKLNV*LDVEKKVTFA*MKLNVISKEEYLLIDKNAKINIGRMLEIENETRHDVVAFTRAISET
LGNEKK*IHLGLTSTDVVDTAQNKLIQESNEVVLESLINLKNTLKEKALIYKKTLTMGRSHGIYGEPTSLGLKFLL*FDE
IDRQIERFSLARKQIEVAKISGSMGNYANLEFEIEEIVAEQMGLGIDNISTQVTQRDRHAFLISVFANIASTLEKIATEI
RLFQRSEVKEWHEGFKANQKGSSSMPHKKNPISSENITGLSRYLRSFTNTAFENNVL*HERDISHSSNERIIFSDVFNVL
VYTLDRLKNTLDDLVIDENKMLEHINSQFNVFYSQPILNYLLINCDKSREEIYDFIQKCTFETMNNKVDFKQSLINNGIE
KYIDNIEVLNKLFNIDYFIRNVEKIYNRTLNK
>Mature_432_residues
MIKRYSVNKIEKI*DENNKLNV*LDVEKKVTFA*MKLNVISKEEYLLIDKNAKINIGRMLEIENETRHDVVAFTRAISET
LGNEKK*IHLGLTSTDVVDTAQNKLIQESNEVVLESLINLKNTLKEKALIYKKTLTMGRSHGIYGEPTSLGLKFLL*FDE
IDRQIERFSLARKQIEVAKISGSMGNYANLEFEIEEIVAEQMGLGIDNISTQVTQRDRHAFLISVFANIASTLEKIATEI
RLFQRSEVKEWHEGFKANQKGSSSMPHKKNPISSENITGLSRYLRSFTNTAFENNVL*HERDISHSSNERIIFSDVFNVL
VYTLDRLKNTLDDLVIDENKMLEHINSQFNVFYSQPILNYLLINCDKSREEIYDFIQKCTFETMNNKVDFKQSLINNGIE
KYIDNIEVLNKLFNIDYFIRNVEKIYNRTLNK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=329, Percent_Identity=24.6200607902736, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI183227688, Length=288, Percent_Identity=26.7361111111111, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1787376, Length=328, Percent_Identity=24.0853658536585, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=364, Percent_Identity=24.4505494505494, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32564234, Length=271, Percent_Identity=27.3062730627306, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323391, Length=371, Percent_Identity=26.4150943396226, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647570, Length=309, Percent_Identity=24.5954692556634, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49251; Mature: 49251

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRYSVNKIEKIDENNKLNVLDVEKKVTFAMKLNVISKEEYLLIDKNAKINIGRMLEIE
CCCCCCCHHHHHCCCCCCEEEEEHHHHEEEEEEEEEECCCCEEEEECCCEEEECEEEEEC
NETRHDVVAFTRAISETLGNEKKIHLGLTSTDVVDTAQNKLIQESNEVVLESLINLKNTL
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKALIYKKTLTMGRSHGIYGEPTSLGLKFLLFDEIDRQIERFSLARKQIEVAKISGSMG
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NYANLEFEIEEIVAEQMGLGIDNISTQVTQRDRHAFLISVFANIASTLEKIATEIRLFQR
CCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVKEWHEGFKANQKGSSSMPHKKNPISSENITGLSRYLRSFTNTAFENNVLHERDISHS
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SNERIIFSDVFNVLVYTLDRLKNTLDDLVIDENKMLEHINSQFNVFYSQPILNYLLINCD
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHEEEEHHHHHHHHHCCCC
KSREEIYDFIQKCTFETMNNKVDFKQSLINNGIEKYIDNIEVLNKLFNIDYFIRNVEKIY
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRTLNK
HHHCCC
>Mature Secondary Structure
MIKRYSVNKIEKIDENNKLNVLDVEKKVTFAMKLNVISKEEYLLIDKNAKINIGRMLEIE
CCCCCCCHHHHHCCCCCCEEEEEHHHHEEEEEEEEEECCCCEEEEECCCEEEECEEEEEC
NETRHDVVAFTRAISETLGNEKKIHLGLTSTDVVDTAQNKLIQESNEVVLESLINLKNTL
CCCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKALIYKKTLTMGRSHGIYGEPTSLGLKFLLFDEIDRQIERFSLARKQIEVAKISGSMG
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NYANLEFEIEEIVAEQMGLGIDNISTQVTQRDRHAFLISVFANIASTLEKIATEIRLFQR
CCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVKEWHEGFKANQKGSSSMPHKKNPISSENITGLSRYLRSFTNTAFENNVLHERDISHS
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SNERIIFSDVFNVLVYTLDRLKNTLDDLVIDENKMLEHINSQFNVFYSQPILNYLLINCD
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHEEEEHHHHHHHHHCCCC
KSREEIYDFIQKCTFETMNNKVDFKQSLINNGIEKYIDNIEVLNKLFNIDYFIRNVEKIY
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRTLNK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA