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The map label for this gene is purA [H]

Identifier: 50364889

GI number: 50364889

Start: 95430

End: 96719

Strand: Direct

Name: purA [H]

Synonym: Mfl074

Alternate gene names: 50364889

Gene position: 95430-96719 (Clockwise)

Preceding gene: 50364888

Following gene: 50364890

Centisome position: 12.03

GC content: 29.61

Gene sequence:

>1290_bases
ATGAGAGAGATAAATTCATTAGTAGTAGTTGGTAGTCAATGAGGAGATGAAGGTAAAGGTAAAATGACAGATTACTTTGC
TCAAAAAGCAGACGTAGTTGTTAGATTTGCTGGTGGTGATAATGCTGGTCATGTTATAAACTTTAATGGCCAAAAACATA
AAGTAACGATTATTCCTTCAGGTATTTTTAATAGCGAAGTAACAAGTGTAATTGGTAATGGTTGTGCTGTAAACTTAATA
AACTTAGTAAAAGAACTTGAAACAATTAAAAACAGTGGAGTTAAGTTAGGTAAACTTTTAATTTCTGATAGAGCTCAATT
AATATTGCCATATCACATATTAATAGATGGTGCTCAAGAAGAATCAAGAGGAGCAAGAAAAATTGGTACAACAAAAAGAG
GTATTGGACCAACTTATCAAGATAAAGCTGCAAGATTAGGAATTAGAGTTGCTGATATTGAAGAAGAAGATTTTAAAGAA
ACATTTAAAGAAATTTTTGAATATCAAATGATGTTCTTGGACAGAATGTTTAATGTTGAAAGCATTGACTTTGAAGAAAC
TTATGCTAATTTAATAAATGCATATAACGTAATTAAAGATTGTGTTACAGATACAGGTATTTTTGTTGAGCAAGCTATTA
AAAATGGTAAAAAAGTTTTATTTGAAGGAGCACAAGGAGCTTTATTAGATATTGATCATGGAACTTATCCATACGTAACT
AGCTCAAATACTTCAGCAAACAATGCATCAACAGGTACAGGTATTTCTCATAAATTAATTAATAATACTTTAGGTGTTGT
AAAAGCTTATTCAACAAGAGTTGGAGCAGGAGCTTTTCCTACTGAATTACTAAATGAAGTTGGTGATGGTATAAGAGAGC
GTGGACATGAGTATGGATCAAATACTAAAAGACCAAGACGTGTTGGATGATTAGATTTAGTTGCATTAAAACATGCAATT
AGAACTTCAGGAATTGATTACTTATTTATAACTCTCTTAGATGTATTATCAGGAGTTGAAGAATTACTTATTTGTGATAA
ATATATTTTAAATGGAGAAGAAATAAATTATATTCCAGCAACAAGTTCTAAACATGAAAAGTGTAAAGCAAATTATATTT
CAATGCCAGGTTGAAAAGAAGATATAACAAAAGTTAAGCATTTCGAAGAATTACCATTAAATGCAAAAAACTATTTAAAT
AAAATTGCCGAAATTTGTGAAGTTGAAATATCTGGATTTTCAGTAGGTCCTGATAGATTACAAACTGTTATAACTAAAGA
AATAATGTAG

Upstream 100 bases:

>100_bases
ACTAAATAAAACTTTGTCAAAAATAACTCAAGTAATACAAAAATAACCTCAAAATGCCTAAAAAGCATCGAGGTTATTTT
TTATTTAGAAAAGGATTTTT

Downstream 100 bases:

>100_bases
AAAATTTAAGGTAAAATATGATAAAAAGATATTCTGTAAATAAGATAGAAAAGATTTGAGACGAAAACAATAAATTAAAT
GTTTGACTTGATGTAGAGAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MREINSLVVVGSQWGDEGKGKMTDYFAQKADVVVRFAGGDNAGHVINFNGQKHKVTIIPSGIFNSEVTSVIGNGCAVNLI
NLVKELETIKNSGVKLGKLLISDRAQLILPYHILIDGAQEESRGARKIGTTKRGIGPTYQDKAARLGIRVADIEEEDFKE
TFKEIFEYQMMFLDRMFNVESIDFEETYANLINAYNVIKDCVTDTGIFVEQAIKNGKKVLFEGAQGALLDIDHGTYPYVT
SSNTSANNASTGTGISHKLINNTLGVVKAYSTRVGAGAFPTELLNEVGDGIRERGHEYGSNTKRPRRVGWLDLVALKHAI
RTSGIDYLFITLLDVLSGVEELLICDKYILNGEEINYIPATSSKHEKCKANYISMPGWKEDITKVKHFEELPLNAKNYLN
KIAEICEVEISGFSVGPDRLQTVITKEIM

Sequences:

>Translated_429_residues
MREINSLVVVGSQ*GDEGKGKMTDYFAQKADVVVRFAGGDNAGHVINFNGQKHKVTIIPSGIFNSEVTSVIGNGCAVNLI
NLVKELETIKNSGVKLGKLLISDRAQLILPYHILIDGAQEESRGARKIGTTKRGIGPTYQDKAARLGIRVADIEEEDFKE
TFKEIFEYQMMFLDRMFNVESIDFEETYANLINAYNVIKDCVTDTGIFVEQAIKNGKKVLFEGAQGALLDIDHGTYPYVT
SSNTSANNASTGTGISHKLINNTLGVVKAYSTRVGAGAFPTELLNEVGDGIRERGHEYGSNTKRPRRVG*LDLVALKHAI
RTSGIDYLFITLLDVLSGVEELLICDKYILNGEEINYIPATSSKHEKCKANYISMPG*KEDITKVKHFEELPLNAKNYLN
KIAEICEVEISGFSVGPDRLQTVITKEIM
>Mature_429_residues
MREINSLVVVGSQ*GDEGKGKMTDYFAQKADVVVRFAGGDNAGHVINFNGQKHKVTIIPSGIFNSEVTSVIGNGCAVNLI
NLVKELETIKNSGVKLGKLLISDRAQLILPYHILIDGAQEESRGARKIGTTKRGIGPTYQDKAARLGIRVADIEEEDFKE
TFKEIFEYQMMFLDRMFNVESIDFEETYANLINAYNVIKDCVTDTGIFVEQAIKNGKKVLFEGAQGALLDIDHGTYPYVT
SSNTSANNASTGTGISHKLINNTLGVVKAYSTRVGAGAFPTELLNEVGDGIRERGHEYGSNTKRPRRVG*LDLVALKHAI
RTSGIDYLFITLLDVLSGVEELLICDKYILNGEEINYIPATSSKHEKCKANYISMPG*KEDITKVKHFEELPLNAKNYLN
KIAEICEVEISGFSVGPDRLQTVITKEIM

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=420, Percent_Identity=42.1428571428571, Blast_Score=305, Evalue=4e-83,
Organism=Homo sapiens, GI34577063, Length=420, Percent_Identity=41.9047619047619, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI40316944, Length=388, Percent_Identity=42.0103092783505, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=43.1924882629108, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25146555, Length=431, Percent_Identity=39.4431554524362, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI25146553, Length=431, Percent_Identity=39.4431554524362, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=40.7834101382488, Blast_Score=293, Evalue=4e-80,
Organism=Drosophila melanogaster, GI21356033, Length=419, Percent_Identity=41.0501193317422, Blast_Score=297, Evalue=8e-81,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46826; Mature: 46826

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00513 ADENYLOSUCCIN_SYN_2 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREINSLVVVGSQGDEGKGKMTDYFAQKADVVVRFAGGDNAGHVINFNGQKHKVTIIPSG
CCCCCCEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEEEECC
IFNSEVTSVIGNGCAVNLINLVKELETIKNSGVKLGKLLISDRAQLILPYHILIDGAQEE
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEEEECCCHH
SRGARKIGTTKRGIGPTYQDKAARLGIRVADIEEEDFKETFKEIFEYQMMFLDRMFNVES
HCCHHHCCCCCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDFEETYANLINAYNVIKDCVTDTGIFVEQAIKNGKKVLFEGAQGALLDIDHGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTSANNASTGTGISHKLINNTLGVVKAYSTRVGAGAFPTELLNEVGDGIRERGHEYGSN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
TKRPRRVGLDLVALKHAIRTSGIDYLFITLLDVLSGVEELLICDKYILNGEEINYIPATS
CCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
SKHEKCKANYISMPGKEDITKVKHFEELPLNAKNYLNKIAEICEVEISGFSVGPDRLQTV
CCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHH
ITKEIM
HHHHCC
>Mature Secondary Structure
MREINSLVVVGSQGDEGKGKMTDYFAQKADVVVRFAGGDNAGHVINFNGQKHKVTIIPSG
CCCCCCEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEEEECC
IFNSEVTSVIGNGCAVNLINLVKELETIKNSGVKLGKLLISDRAQLILPYHILIDGAQEE
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEEEECCCHH
SRGARKIGTTKRGIGPTYQDKAARLGIRVADIEEEDFKETFKEIFEYQMMFLDRMFNVES
HCCHHHCCCCCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDFEETYANLINAYNVIKDCVTDTGIFVEQAIKNGKKVLFEGAQGALLDIDHGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNTSANNASTGTGISHKLINNTLGVVKAYSTRVGAGAFPTELLNEVGDGIRERGHEYGSN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
TKRPRRVGLDLVALKHAIRTSGIDYLFITLLDVLSGVEELLICDKYILNGEEINYIPATS
CCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
SKHEKCKANYISMPGKEDITKVKHFEELPLNAKNYLNKIAEICEVEISGFSVGPDRLQTV
CCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHH
ITKEIM
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA