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The map label for this gene is prs [H]

Identifier: 50364891

GI number: 50364891

Start: 98070

End: 99113

Strand: Direct

Name: prs [H]

Synonym: Mfl076

Alternate gene names: 50364891

Gene position: 98070-99113 (Clockwise)

Preceding gene: 50364890

Following gene: 50364892

Centisome position: 12.36

GC content: 29.98

Gene sequence:

>1044_bases
ATGCTTTCAAAAAAAGAAAATGTACATATATTTGGTTTAACTCAAGGGGTTGAACTAGCACAAGAAATTTGTGATATTTT
AGGGGTAAAAAGAAAAGAAGTTAAAACTTTAAAATTTGCTGATGGTGAAATATTAATAGAATCTTTAGACTCAGTTAGAG
GTAAAGAAATCTTTGTAATACAATCAACATCAACACCAGTAAATGAAAGTATAATGGAATTATTAATTGCTATTGATGCT
TTCAAAAGAGGAAGCGCTGAAAAAATTAATGTAGTTATTCCTTACTATGGTTATGCAAGACAAGATAGAAAAGCCAAAGG
AAGACAACCAATTACATCAAAATTAGTTGCTGACTTATTAACTAAAGCAGGTGCTGACAGAGTTATGTCAATTGATATTC
ACTCTCCACAATCAATGGGATTCTTTGATGTTCCAATGGATAATTTCTATACAGCTCAAACATTAGCAACTGAAATTATA
GATACAATTGAATTAAATAATTGAGACCCTTCTAATTGTATTTTAGTTTCTCCAGATTATGGTGGAATGACAAGGGTTCA
TAAAGTTGAATCTTACACAGGTGGTGTAACTAATGGAATTGCAGTTATTGGTAAAAGAAGGCCAGAACCTAATAAAGCTG
AAGTTGAATTTGTTCTAGGTGATATAAAAGACAAACATTGTTTCATCATTGATGACATGATAGACACAGGCGGGACAATA
ATTAACGCAGCAAAAGCATTAAAAGAACAAGGCGCTAAAGATGTACATATTTTTGCTTGTCATGGTTTATTTAATGGGCC
TGCAAAAGAAAGAATGGAAGCAGCAATTAAAGAAAAAATAGTTGAAGAAGTTGTAGTTACTAATACAATTCAAATAGCTG
ATGAAAAAAAATTCAATGGATTAAAAATTATTTCAGTGGCTCCTCTATTAGCAGAAATGATTGATTCATCAATCAGCCAT
GAATCTTTAACTGAAGTTTATAATAATAAAAAACAAATAATTTGAGATAGAGCTCAATGTATTGTTAATAAATTTAAAAA
ATAA

Upstream 100 bases:

>100_bases
CTTTTTAATATTGATTACTTCATAAGAAATGTTGAGAAGATATATAACAGAACATTAAATAAATAATTTGATATTATAAA
AAAAAGGGGAAAAATTAAAA

Downstream 100 bases:

>100_bases
TTAAGAGGAATATAAAATGAAATTAATAGTAGGATTAGGAAATTATGGTTCTCAATATGAAGCTACAAGACATAATGCTG
GTTGAATAGCATTAGATCAG

Product: phosphoribosylpyrophosphate synthetase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLSKKENVHIFGLTQGVELAQEICDILGVKRKEVKTLKFADGEILIESLDSVRGKEIFVIQSTSTPVNESIMELLIAIDA
FKRGSAEKINVVIPYYGYARQDRKAKGRQPITSKLVADLLTKAGADRVMSIDIHSPQSMGFFDVPMDNFYTAQTLATEII
DTIELNNWDPSNCILVSPDYGGMTRVHKVESYTGGVTNGIAVIGKRRPEPNKAEVEFVLGDIKDKHCFIIDDMIDTGGTI
INAAKALKEQGAKDVHIFACHGLFNGPAKERMEAAIKEKIVEEVVVTNTIQIADEKKFNGLKIISVAPLLAEMIDSSISH
ESLTEVYNNKKQIIWDRAQCIVNKFKK

Sequences:

>Translated_347_residues
MLSKKENVHIFGLTQGVELAQEICDILGVKRKEVKTLKFADGEILIESLDSVRGKEIFVIQSTSTPVNESIMELLIAIDA
FKRGSAEKINVVIPYYGYARQDRKAKGRQPITSKLVADLLTKAGADRVMSIDIHSPQSMGFFDVPMDNFYTAQTLATEII
DTIELNN*DPSNCILVSPDYGGMTRVHKVESYTGGVTNGIAVIGKRRPEPNKAEVEFVLGDIKDKHCFIIDDMIDTGGTI
INAAKALKEQGAKDVHIFACHGLFNGPAKERMEAAIKEKIVEEVVVTNTIQIADEKKFNGLKIISVAPLLAEMIDSSISH
ESLTEVYNNKKQII*DRAQCIVNKFKK
>Mature_347_residues
MLSKKENVHIFGLTQGVELAQEICDILGVKRKEVKTLKFADGEILIESLDSVRGKEIFVIQSTSTPVNESIMELLIAIDA
FKRGSAEKINVVIPYYGYARQDRKAKGRQPITSKLVADLLTKAGADRVMSIDIHSPQSMGFFDVPMDNFYTAQTLATEII
DTIELNN*DPSNCILVSPDYGGMTRVHKVESYTGGVTNGIAVIGKRRPEPNKAEVEFVLGDIKDKHCFIIDDMIDTGGTI
INAAKALKEQGAKDVHIFACHGLFNGPAKERMEAAIKEKIVEEVVVTNTIQIADEKKFNGLKIISVAPLLAEMIDSSISH
ESLTEVYNNKKQII*DRAQCIVNKFKK

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=323, Percent_Identity=39.0092879256966, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI28557709, Length=323, Percent_Identity=39.3188854489164, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4506129, Length=323, Percent_Identity=38.6996904024768, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI84875539, Length=326, Percent_Identity=38.3435582822086, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4506133, Length=344, Percent_Identity=31.3953488372093, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI194018537, Length=346, Percent_Identity=30.635838150289, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1787458, Length=322, Percent_Identity=40.3726708074534, Blast_Score=255, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI25149168, Length=323, Percent_Identity=37.1517027863777, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17554702, Length=323, Percent_Identity=37.1517027863777, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI71989924, Length=326, Percent_Identity=36.8098159509202, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17554704, Length=320, Percent_Identity=37.1875, Blast_Score=207, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=30.0884955752212, Blast_Score=151, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321776, Length=312, Percent_Identity=38.1410256410256, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319403, Length=319, Percent_Identity=35.7366771159875, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6320946, Length=312, Percent_Identity=35.2564102564103, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6322667, Length=168, Percent_Identity=41.0714285714286, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324511, Length=273, Percent_Identity=27.8388278388278, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355239, Length=327, Percent_Identity=39.7553516819572, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI45551540, Length=350, Percent_Identity=37.1428571428571, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=29.0140845070423, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=29.0140845070423, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=29.0140845070423, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=29.0140845070423, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI45552010, Length=374, Percent_Identity=28.6096256684492, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24651462, Length=374, Percent_Identity=28.6096256684492, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24651464, Length=374, Percent_Identity=28.6096256684492, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 38076; Mature: 38076

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKKENVHIFGLTQGVELAQEICDILGVKRKEVKTLKFADGEILIESLDSVRGKEIFVI
CCCCCCCEEEEEECCHHHHHHHHHHHHCCCHHHHCEEEECCCHHHHHHHHCCCCCEEEEE
QSTSTPVNESIMELLIAIDAFKRGSAEKINVVIPYYGYARQDRKAKGRQPITSKLVADLL
ECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHH
TKAGADRVMSIDIHSPQSMGFFDVPMDNFYTAQTLATEIIDTIELNNDPSNCILVSPDYG
HHCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
GMTRVHKVESYTGGVTNGIAVIGKRRPEPNKAEVEFVLGDIKDKHCFIIDDMIDTGGTII
CCHHHEEHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEECHHHCCCHHH
NAAKALKEQGAKDVHIFACHGLFNGPAKERMEAAIKEKIVEEVVVTNTIQIADEKKFNGL
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCE
KIISVAPLLAEMIDSSISHESLTEVYNNKKQIIDRAQCIVNKFKK
EEEHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSKKENVHIFGLTQGVELAQEICDILGVKRKEVKTLKFADGEILIESLDSVRGKEIFVI
CCCCCCCEEEEEECCHHHHHHHHHHHHCCCHHHHCEEEECCCHHHHHHHHCCCCCEEEEE
QSTSTPVNESIMELLIAIDAFKRGSAEKINVVIPYYGYARQDRKAKGRQPITSKLVADLL
ECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHH
TKAGADRVMSIDIHSPQSMGFFDVPMDNFYTAQTLATEIIDTIELNNDPSNCILVSPDYG
HHCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
GMTRVHKVESYTGGVTNGIAVIGKRRPEPNKAEVEFVLGDIKDKHCFIIDDMIDTGGTII
CCHHHEEHHHHCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCEEEEEECHHHCCCHHH
NAAKALKEQGAKDVHIFACHGLFNGPAKERMEAAIKEKIVEEVVVTNTIQIADEKKFNGL
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCE
KIISVAPLLAEMIDSSISHESLTEVYNNKKQIIDRAQCIVNKFKK
EEEHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]